Improved Methods for

Forensic Toxicology Analysis

Specialized Products for Forensic Toxicology T he

screening and confirmation for drugs of abuse and their metabolites present many challenges in toxicological analysis. Searching for illegal drugs in complex matrices, such as urine and blood, can prove to be difficult because if present, they may be found at very low concentration levels. Direct analysis of these samples is often hampered by endogenous compounds in the biomatrix. These interferences are often in higher concentrations than that of the target analytes and thus may mask their presence.

W

e at Phenomenex understand your needs and have therefore developed products that allow you to get your work done with confidence. Phenomenex SPE, GC/MS, HPLC, and LC/MS products have the phases and formats you need in order to detect drugs of abuse from various matrices. We use the latest technology to ensure our products will give you the highest sensitivity and fastest analysis time every time.

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Table of Contents Specialized Products for Forensic Toxicology-----pp. 2-6 Sample Preparation/SPE------------------------------------------- 2-3 GC/MS--------------------------------------------------------------- 4-5 LC/MS-------------------------------------------------------------------6

Applications---------------------------------------- pp. 8-25 PCP----------------------------------------------------------------------8 Methadone--------------------------------------------------------------9 THC----------------------------------------------------------------- 10-11 Cocaine------------------------------------------------------------ 12-13 Amphetamines ---------------------------------------------------- 14-15 Opiates-------------------------------------------------------------16-17 Steroids-------------------------------------------------------------18-19 Benzodiazepines------------------------------------------------- 20-21 Barbiturates------------------------------------------------------- 22-23 6-MAM---------------------------------------------------------------- 24 Blood Alcohols-------------------------------------------------------- 25

Ordering Information------------------------------ pp. 28-33 Sample Preparation/SPE Sorbents-------------------------------- 28 GC Columns---------------------------------------------------------- 29 General GC Accessories---------------------------------------- 30-31 HPLC Columns--------------------------------------------------- 32-33 Key Premier Solutions: Products providing the optimal results for the analysis Standard Solutions: Products for general routine work Low Cost Solutions: Alternative products for labs interested in cost savings

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products

Specialized

p. 2

Sample Preparation/SPE

Effective Extraction and High Recovery Low Cost Solutions

Strata® Silica-Based SPE Sorbents • Consistent manufacturing for accurate and reproducible results • Inert material provides clean extracts • Standing orders available

Strata Screen-C

Strata Screen-C GF

Mixed-mode Cation Exchange

Improved Gravity Flow

• Extraction of hydrophobic and basic drugs from biological matrices such as blood, serum, and urine

• Large particle for clog-free, superior flow in absence of a vacuum

• Removes anionic and neutral interferences without seriously affecting the recovery of the basic analyte

• Good for viscous samples such as horse urine and plasma, or gravity flow applications • High extraction efficiency compared to traditional mixed-mode sorbents of smaller particle size

Alternative for:

Alternative for:

Varian Bond Elut Certify® I, UCT Clean Screen® DAU, JT BAKER Bakerbond Narc™-2, IST ISOLUTE® HCX, Macherey-Nagel Chromabond Drug

Varian Bond Elut Certify® HF, UCT XtrackT® DAU

Contact Phenomenex or your local Phenomenex distributor for Strata Screen-C GF ordering information.

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Specialized

Sample Preparation/SPE

products

p. 3

Premier Solutions Strata™-X Polymer-Based SPE Sorbents • Lot-to-lot reproducibility provides consistent recoveries from extraction-to-extraction

Strata-X is de-condition resistant for higher recovery, precision, & ruggedness % Recovery

120

• pH stability from 1-14 for safe and stable basic elution of many drug compounds • High analyte capacity for loading up to 66 % more in comparison to silica-based sorbents per gram

100 80 60

Strata-X

40

C18

20 0 0

1

2

4

Drying Time (min)

Strata-X-C

Strata-XL-C

Strong Cation Exchange and Reversed Phase

Improved Gravity Flow

• Withstands high organic washes to more completely remove matrix contaminants

• Large particle size provides excellent flow for viscous biological fluids such as horse urine and plasma

• Attain better LODs/LOQs for increased sensitivity, accuracy, and precision of data

• High recovery of drugs using the same selectivity as Strata-X-C

• pH limit 1-14 offers more controlled selectivity and higher recoveries of analytes

• Consistent tube-to-tube flow

• Eliminate the need for harsh halogenated solvents

An alternative for: Waters Oasis® MCX, UCT’s StyreScreen® DBX

Strata-X

Strata-XL

• Controlled selectivity for target analytes for removal of contaminants and better elimination of matrix effects

• Large particle size provides excellent flow for viscous biological fluids such as horse urine and plasma

• High analyte capacity for loading up to 66 % more in comparison to silica-based sorbents per gram

• High recovery of drugs using the same selectivity as Strata-X

• Withstands aggressive organic washes ( 57 - 60 % organic)* without breakthrough of analyte

• Consistent tube-to-tube flow

Reversed Phase

Improved Gravity Flow

• Reproducible recoveries from extraction-to-extraction and superior lot-to-lot reproducibility

An alternative for: Waters Oasis® HLB, Varian NEXUS, FOCUS and PLEXA, UCT’s StyreScreen® DVB, JT Baker H20-pilic DVB, IST ENV+, Macherey-Nagel’s Easy and HR-X

For Strata-X on-line extraction cartridges, see p. 28.

*Please see TN-0017, "Testosterone on Strata-X vs. C18-E"

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GC/MS

NEW

GC Column Dimensions for Fast Drug Analysis

Standard Solution NEW Zebron™ GC Columns Developed for Forensic Testing Phenomenex now offers new optimized column dimensions (10 meter x 0.18 mm x 0.18 µm) in our award winning Zebron GC column phases. This special dimension allows for a major improvement in sample throughput, while still providing great peak shapes and resolution. In addition, the extended temperature stability range and the low column bleed levels allow for high temperature removal of matrix contamination and fast elution of target analytes. • Decrease run time by at least 30 % • Symmetrical peak shapes and great resolution for drugs of abuse • Better limits of detection (LOD) and quantitation (LOQ)

New Optimized Column Dimensions Available in These Popular Phases: Zebron ZB-5MSi - Specially deactivated for analysis of ZB-5ms - Provides improved resolution for aromatic

derivatized and un-derivatized drug compounds.

compounds

Alternative for:

Alternative for:

ZB-1ms -

ZB-XLB -

DB-5, HP-5msi, Rtx-5ms, Rtx-5Amine, HP-5ms

DB-5ms, DB-5ms EVDX, VF-5ms, CP-Sil 8 CB MS, DB-5.625

Good low polarity phase for general drug screening.

unique phase that can be used to improve resolution of structurally similar compounds

Alternative for:

Alternative for:

DB-1ms, HP-1ms, Rtx-1ms, VF-1ms, MDN-1, Equity-1, AT-1ms

DB-XLB, Rtx-XLB, MDN-12, VF-Xms

Reduce analysis time! Optimized Dimension

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detector: Sample:

17902 1, 2

Zebron ZB-5MSi 15 meter x 0.25 mm x 0.25 μm 7EG-G018-11 Split 10:1 @ 260 °C, 1 μL Helium @ 1.5 mL/min (constant flow­) 150 °C to 320 °C @ 25 °C/min MSD @ 320 °C Analytes @ 1.2 ppm BSTFA 1. Carboxy-THC-d3 2. Carboxy-THC

App ID 17791

Traditional Dimension App ID 17902

products

Specialized

p. 4

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detector: Sample:

17791 1,2

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 μm 7CD-G018-08 Split 10:1 @ 280 °C, 1 μL Helium @ 0.7 mL/min (constant flow­) 200 °C to 300 °C @ 20 °C/min MSD @ 320 °C Analytes @ 1.2 ppm BSTFA 1. Carboxy-THC-d3 2. Carboxy-THC

30 % Faster Run Time 4.7

4.8

5.0

5.2

5.4

5.6

5.8

6.0

6.2

6.4

6.6

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6.7 min

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3.0

3.1

3.2

3.3

3.4

3.5

3.6

3.7

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3.8

3.9

4.0

4.1

4.2

4.3

4.4

4.5

4.6 min

GC/MS

Specialized

NEW GC Columns Designed for Drugs of Abuse

Premier Solution NEW Zebron™ ZB-Drug-1 is a Specialized Solution Developed for Forensic Testing Zebron ZB-Drug-1 is a new mid polarity phase designed to provide fast analysis for compounds in drugs of abuse. The unique stationary phase improves separation of target analytes from common matrix interferences present in biological samples. The special deactivation procedure improves peak shape for acidic and basic drugs, allowing for more accurate quantitation. MS certified bleed levels allow for low level detection. • Specially deactivated to improve quantitation for drug compounds • New 10 meter x 0.18 mm x 0.18 µm dimensions significantly reduces run time • Temperature Limits (-60 to 320/340 °C)

Zebron ZB-Drug-1: Faster Results, Better Peak Shape, Great Separation

Opiates on a ZB-Drug-1

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detector: 3,4 Sample:

16733

1,2

Zebron ZB-5ms 10 meter x 0.18 mm x 0.18 μm 7CD-G018-08-C Split 10:1 @ 240 °C, 1 μL Helium @ 1.2 mL/min (constant flow­) 140 °C to 320 °C @ 20 °C/min for 1 min MSD @ 230 °C Analytes @ 10 ppm BSTFA/Pyridine 1. Codeine-d6 2. Codeine 3. Morphine-d3 4. Morphine

App ID 17806

App ID 16733

Opiates on a ZB-5ms

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detector: Sample:

17806

3,4

4.7

4.8

4.9

5

5.1

5.2

5.3

5.4

5.5

Web:

5.6

5.7

5.8

5.9

6 min

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4.0

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 μm 7CD-G023-08 Split 10:1 @ 280 °C, 1 μL Helium @ 55 cm/sec (constant flow­) 180 °C to 340 °C @ 20 °C/min MSD @ 230 °C Analytes @ 10 ppm BSTFA/Pyridine 1. Codeine-d6 2. Codeine 3. Morphine-d3 4. Morphine

2

Unique Selectivity for Drugs

1

4.1

4.2

4.3

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4.4

4.5

4.6

products

p. 5

4.7

4.8

min

LC/MS

HPLC Columns for LC/MS Analysis

Premier Solutions

Phenomenex HPLC Columns Phenomenex’s LC/MS columns are designed to give high sensitivity, optimal retention, and a full range of selectivities for drugs of abuse. Our columns have low MS bleed, which results in less background noise. This is important when quantitating samples in low concentration.

Gemini®

• Achieve high sensitivity, optimal retention, and great separation of basic drugs • Positively impact laboratory productivity with longer column lifetime • Extended pH stability 1-12

Luna®

• High quality phases good for all types of forensic samples • Low MS bleed improves sensitivity for LC/MS/MS methods • Extensive batch traceability and reproducibility data supplied with every column

Synergi ™

• Unique phases that provide enhanced selectivity across the polarity scale • Often resolves compounds that co-elute on traditional column chemistries • Synergi Hydro-RP (C18 with Polar Endcapping) has maximum hydrophobic selectivity with added polar retention. Also compatible with 100 % aqueous conditions

MercuryMS™

• Ultra-fast, low-cost analysis for high-throughput LC/MS laboratories • Packed with Luna, Synergi, and Gemini material • Short 10 and 20 mm cartridge formats use a new proprietary slurry packing process

Cocaine and Metabolites App ID 17914

products

Specialized

p. 6

Column: Dimensions: Part No.: Mobile Phase:

17914

4

Gradient:

3

Flow Rate: Detection: Sample:

2

1 5

0

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2

3

4

5

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6

Luna 3 µm HILIC 100 x 2.0 mm 00D-4449-B0 A: Acetonitrile/ 100 mM Ammonium formate, pH 3.2 (50:45:5) B: Acetonitrile/ Water/ 100 mM Ammonium formate pH 3.2 (50:45:5) A/B (100:0) for 1.7 min, to A/B (45:55) in 3.1 min, hold for 1.7 min, then to A/B (100:0) for 6 min 0.4 mL/min Mass Spectrometer (MS) 1. Cocaine 2. Norcocaine 3. Ecgonin Methyl Ester 4. Benzoylecgonine 5. Ecgonine

7

8

9

10

11

12 min

Applications

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PCP

PCP Extraction of Phencyclidine from Urine and Analysis by GC/MS Phencyclidine (PCP) induces hallucinogenic and neurotoxic effects. Since a vast majority of the parent drug and its metabolite are excreted in urine, abuse is determined by detecting the parent drug.

Solutions SPE

Premier Solution: Strata -X-C Low Cost Solution: Strata® Screen-C

GC/MS

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

™

PCP is a weakly basic compound with a pKa of about 8.5, but a relatively high LogP of 5.32. The pKa and high LogP allow it to be effectively retained through hydrophobic and ionic interactions on mixed-mode cation-exchange sorbent such as Screen-C or Strata-X-C. The high hydrophobic retention of PCP can reduce recoveries if strong organic elution is not used.

Derivatization of this compound is not necessary as the amine functionality does not display adverse secondary interactions in the GC system. The single peak is easily analyzed with good sensitivity.

GC/MS Cut off 25 ng/mL Derivatization n/a Mass Ion(s)

PCP

Quant Ion

Secondary Ion

Tertiary Ion

PCP

200

243

186

PCP - d5 (IS)

205

248

App ID 17800

Phencyclidine on a Zebron™ ZB-Drug-1 Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17800

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 280 °C, 0.5 µL Helium @ 55 cm/sec (constant flow) 160 °C to 340 °C @ 20 °C/min MSD @ 230 °C Analytes at 2 ppm in Ethyl acetate 1. Phencyclidine-d5 2. Phencyclidine

1,2

Recovery of PCP by SPE 110 100

103

90 80

2.0

2.2

2.4

2.6

2.8

3.0

3.2

3.4

3.6

3.8

4.0

4.2

4.4

4.6

% Recovery

70

min

17787 1,2

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

60 50 40 30

Phencyclidine on a Zebron™ ZB-5MSi App ID 17787

Applications

p. 8

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1 @ 260 °C, 1 µL Helium @ 0.6 mL/min (constant flow) 150 °C to 320 °C @ 20 °C/min MSD @ 230 °C Analytes at 2 ppm in Ethyl acetate 1. Phencyclidine-d5 2. Phencyclidine

20 10 0 Strata-X-C

SPE Method Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Dry: Elute: Evaporate: Reconstitute:

1.4

1.6

1.8

2.0

2.2

2.4

2.6

2.8

3.0

3.2

3.4

Web:

3.6

3.8

4.0

4.2

4.4

4.6

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Strata-X-C, 60 mg/3 mL 8B-S029-UBJ 2 mL methanol followed by 2 mL water 4 mL (1 mL of analyte and IS spiked human urine acidified with 2 % H3PO4; diluted with 3 mL 100 mM KH2PO4, pH 6.0) sample 2 mL 0.1 N HCI 2 mL methanol 1 min under 10 in. Hg 2 mL 5 % NH4OH/Methanol (2 x 1 mL) Under nitrogen 300 µL Ethyl acetate

min

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O

Methadone

Methadone

Extraction of Methadone from Urine and Analysis by GC/MS

Applications

p. 9

Methadone binds with opiate receptors and displays many of the same physiological affects as heroin. However, it is not structurally related to heroin or other opiate compounds. To determine abuse, a separate screening method must be performed.

Solutions SPE

Premier Solution: Strata -X-C Low Cost Solution: Strata® Screen-C

GC/MS

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

™

Methadone is a non-polar basic compound that is well retained on cation-exchangers, such as Strata® Screen-C and Strata X-C, or on neutral sorbents such as Strata-X.

Methadone can be analyzed by GC/MS, similar to other drugs. Derivatization improves peak shape by decreasing reactivity with system components.

Methadone

GC/MS Cut off 300 ng/mL Derivatization n/a Mass Ion(s)

O

Quant Ion

Secondary Ion

Methadone-d9 (IS)

78

303

Tertiary Ion

 

Methadone

72

294

223

N

App ID 17804

Methadone on a Zebron™ ZB-Drug-1 Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17804 2

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 260 °C, 1 µL Helium @ 55 cm/sec (constant flow) 200 °C to 300 °C @ 25 °C/min MSD @ 230 °C 24 ppm in Ethyl acetate 1. Methadone-d9 2. Methadone

1

Recovery of Methadone by SPE 100 90

95

80

0.8

1.0

1.2

1.4

1.6

1.8

2.0

2.2

2.4

2.6

2.8

3.0

3.2

3.4

3.4 min

17790 2

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

1

60 50 40 30

Methadone on a Zebron™ ZB-5MSi App ID 17790

0.6

% Recovery

70

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1@ 280 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 200 °C to 300 °C @ 20 °C/min MSD @ 320 °C 24 ppm in Ethyl acetate 1. Methadone-d9 2. Methadone

20 10 0 Strata-X-C

SPE Method Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Elute: Evaporate: Reconstitute:

.80

1.0

1.2

1.4

1.6

1.8

2.0

2.2

Web:

2.4

2.6

2.8

Strata-X-C, 60 mg/3 mL 8B-S029-UBJ 2 mL methanol followed by 2 mL water 3 mL urine spiked with analytes and IS (0.5 µg/mL); dilute 1:1 with 100 mM KH2PO4 (pH 6); acidified with 2 % phosphoric acid 2 mL 0.1 N HCI 2 mL methanol 2 mL 5 % NH4OH/Methanol (2 x 1 mL) Under nitrogen 200 µL Ethyl acetate

Please inquire about other applications.

3.0 min

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N

THC

THC Extraction of ∆9–Carboxy-THC from Urine and Analysis by LC/MS or GC/MS Tetrahydrocannabinol (THC), the main active chemical in marijuana, is mainly metabolized to 9-carboxy-11-Nor-∆9-carboxy-THC (THC-COOH). This compound is then conjugated with glucuronic acid and excreted in urine.

Solutions Premier Solution: Strata™-X-C Low Cost Solution: Strata® Screen-A or -C SPE

Though THC is best extracted using mixed-mode anion-exchange sorbents, such as Strata Screen-A, for convenience many labs will use a mixed-mode cation-exchange sorbent since they are already using this type of sorbent for the majority of their other methods. THC-COOH and THC are effectively extracted from Strata-X-C and Screen-C through disruption of hydrophobicity and hydrogen bonding mechanisms. Removal of more polar matrix interferences can still be effectively done by washing with an organic solution acidified at or below pH 3.

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi GC/MS

In many countries, THC is the most commonly screened drug. Due to the high volume of samples, system stability and fast run time are critical. Starting the GC oven at >200 oC did not affect peak shape or quantitation of THC-COOH, but allowed for much faster cycle times significantly improving sample throughput.

Premier Solution: Gemini® C18 LC/MS

Acidic compounds, such as THC-COOH are often run under mildly basic conditions in negative ion mode. Gemini allows the user an extended pH range to increase basic conditions much further, and create more favorable mass spec ionization for acids and neutrals in a mixture together. In this application, at pH 10, both the THC neutral parent and the THC-COOH metabolite are successfully de-protonated for optimum MS sensitivity in negative ion mode..

THC-COOH

OH OH

H

O O H

Recovery of THC by SPE 100 90

99

93

SPE Method Sorbent: Part No.: Condition: Load:

80 70 % Recovery

Applications

p. 10

Wash 1: Wash 2: Dry:

60 50

Elute: Dry: Reconstitute:

40 30 20 10 0 Strata-X-C

Strata Screen-C

SPE Method Sorbent: Part No.: Condition: Load:

Wash 1: Wash 2: Dry: Elute: Evaporate: Reconstitute:

Web:

Strata-X-C, 60 mg/3 mL 8B-S029-UBJ 2 mL methanol followed by 2 mL 0.1 N HCl Hydrolyzed urine sample Base hydrolysis: 2 mL urine spiked with analyte and IS, add 100 µL 10 N KOH. Mix, vortex and hydrolyze for 20 min at 60 °C. Cool and adjust pH to 3.5-4.0 (by adding 200 µL glacial acetic acid) 2 mL 0.1 N HCl 2 mL 0.1 N HCl/acetonitrile (70:30) 4 min under vacuum. To further remove water: add 200 µL glacial acetic acid, letting it percolate under gravity for 30-40 sec. Dry sorbent for 1 min under vacuum. 2 mL 2 % glacial acetic acid/acetonitrile (2 x 1 mL) Blow down to dryness under N2 @ 40 °C Add 30 µL 10 mM imidazole/ethylacetate + 30 µL BSTFA (1 % TMCS). React at 70 °C for 20 min.

OH

O

Strata Screen-C, 150 mg/3 mL 8B-S016-SBJ 2 mL methanol followed by 2 mL 50 mM phosphoric acid Hydrolyzed urine sample Base hydrolysis: 2 mL urine spiked with analyte and IS, add 300 µL 10 N KOH. Mix, vortex and hydrolyze for 20 min at 60 ºC. Cool and adjust pH to 4-5 (by adding 165 µL glacial acetic acid followed by addition of 2 mL 0.1 M sodium acetate (pH 7)/methanol (95:5) 3 mL 50 mM phosphoric acid 3 mL 50 mM phosphoric acid/methanol (80:20, v:v) 3 min under vacuum; add 0.5 mL hexane followed by additional drying for 1 min 2 mL hexane/ethyl acetate (80:20, v:v) Nitrogen @ 40 ºC Add 25 µL ethyl acetate and 25 µL BSTFA (1 % TMCS). React @ 70 ºC for 20 min.

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THC

THC and Metabolite on Gemini® C18 App ID 17901

Column: Dimensions: Part No.: Mobile Phase: Gradient: Flow Rate: Detection: Sample: 17901

Gemini 3 µm C18 50 x 3.0 mm 00B-4439-Y0 A: 10 mM NH4HCO3 (pH 10) B: Acetonitrile A/B (85:15) to A/B (5:95) in 2.9 min, hold 30 sec, then to A/B (85:15) hold for 30 sec 0.8 mL/min MS @ 450 °C 1. 11-nor-9-Carboxy-THC (THC-COOH) 2. ∆-9-tetrahydrocannabinol (THC)-d3 3. ∆-9-tetrahydrocannabinol (THC)

GC/MS Cut off Derivatization

2

Mass Ion(s)

Quant Ion

Secondary Ion

Carboxy-∆ -THC

371

473

474 

D3-Carboxy-∆9-THC (IS)

374

476

477

3

1

15 ng/mL BSTFA (with 1 % TMCS)

9

Tertiary Ion

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

min

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample: 17801 1,2

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 280 °C, 0.1 µL Helium @ 55 cm/sec (constant flow) 220 °C to 340 °C @ 20 °C/min MSD @ 320 °C Analytes at 1.5 ppm in BSTFA 1. Carboxy-THC-d3 2. Carboxy-THC

OH 2.5

H

2.6

2.7

2.8

2.9

3.0

3.2

3.3

3.40

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: 1,2 Sample:

17791

O H

3.1

3.5

3.6

3.7

3.8

3.9

min

Carboxy-THC on a Zebron™ ZB-5MSi App ID 17791

0

App ID 17801

Carboxy-THC on a Zebron™ ZB-Drug-1

3.0

3.1

3.2

3.3

3.4

3.5

3.6

3.7

3.8

3.9

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1 @ 280 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 200 °C to 300 °C @ 20 °C/min MSD @ 320 °C Analytes at 1.2 ppm in BSTFA 1. Carboxy-THC-d3 2. Carboxy-THC

4.0

4.1

4.2

4.3

4.4

4.5

4.6 min

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Applications

p. 11

Cocaine

Cocaine Extraction of Benzoylecgonine from Urine and Analysis by LC or GC/MS Cocaine is extensively metabolized by the liver, with only 1 % being excreted in the urine unchanged. Abuse is thus detected by monitoring its main metabolite, benzoylecgonine.

Solutions Premier Solution: Strata™-X or Strata-X-C Low Cost Solution: Strata® or Strata Screen-C

Benzoylecgonine contains both an amine and carboxylic acid functionality allowing it to be retained through multiple mechanisms. Typically, benzoylecgonine is extracted using a mixed-mode cation-exchanger such as Strata Screen-C or Strata X-C. However, the ammonium hydroxide used for elution on cation-exchangers causes hydrolysis of the methyl ester in cocaine and cocaethylene converting them into carboxylic acid containing benzoylecgonine metabolite. Using a neutral sorbent such as Strata-X does not require high pH elution, allowing for independent analysis of all three cocaine compounds.

SPE

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

N

GC/MS

The single compound of benzoylecgonine eluted in just over 3 minutes by using a fast temperature ramp. To ensure all matrix contamination is removed, a final oven temperature of 320 oC was used. Overall cycle time is kept to a minimum due to the higher starting oven conditions, which reduce the need for GC cool down time.

O

Premier Solution: Luna® HILIC

LC/MS

Using a HILIC (Hydrophilic-Interaction-Liquid-Chromatography) LC/MS column, cocaine and its metabolites will elute in less than 7 minutes. Adequate separation is achieved for the key metabolites for further detection by MS.

Recovery of Cocaine by SPE 100 90

97

101 99 92

80

91

94

88 89 82

60 50

0

Cocaine and Metabolites Column: Dimensions: Part No.: Mobile Phase: 17914

Gradient:

4

Flow Rate: Detection: Sample:

3 2

1

Luna 3 µm HILIC 100 x 2.0 mm 00D-4449-B0 A: Acetonitrile/ 100 mM Ammonium formate, pH 3.2 (50:45:5) B: Acetonitrile/ Water/ 100 mM Ammonium formate pH 3.2 (50:45:5) A/B (100:0) for 1.7 min, to A/B (45:55) in 3.1 min, hold for 1.7 min, then to A/B (100:0) for 6 min 0.4 mL/min Mass Spectrometer (MS) 1. Cocaine 2. Norcocaine 3. Ecgonin Methyl Ester 4. Benzoylecgonine 5. Ecgonine

5

0

1

2

3

4

5

6

7

Web:

8

9

10

11

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12 min

I Email:

Strata-X

SPE Method Sorbent: Part No.: Condition: Load: Wash: Dry: Elute: Evaporate: Reconstitute: SPE Method Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Dry: Elution: Evaporate: Reconstitute:

Strata-X-C

Cocaethylene

Cocaine

Benzoylecgonine

Cocaine

Cocaethylene

10

Benzoylecgonine

20

Cocaine

30

Cocaethylene

40 Benzoylecgonine

% Recovery

70

App ID 17914

Applications

p. 12

O

Strata Screen-C

Strata-X, 30 mg/1 mL 8B-S100-TAK 1 mL methanol / 1 mL water 1 mL spiked Porcine plasma acidified with 2 % H3PO4 (Diluted 1:2 with water) 1 mL 20 % methanol in water 1 min at 10 in. of Hg 1 mL methanol Under nitrogen @ 40 oC Depends on analysis technique

Strata Screen-C, 150 mg/3 mL 8B-S016-SBJ 2 mL methanol followed by 2 mL water 2 mL porcine plasma spiked with analytes diluted 1:2 with water 2 mL 0.1 N HCl 2 mL methanol 1 min under vacuum 5 % ammonium hydroxide in methanol Under nitrogen @ 40 ºC Depends on analysis technique

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O

Benzoylecgonine

O N

OH

O GC/MS Cut off Derivatization

150 ng/mL BSTFA (with 1 % TMCS)

Mass Ion(s)

Quant Ion

Secondary Ion

Tertiary Ion

D3-Benzoylecgonine (IS)

243

259

364 

Benzoylecgonine

240

256

O

361

Cocaethylene

O

Benzoylecgonine on a Zebron ZB-Drug-1 App ID 17805

™

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17805

1,2

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 260 °C, 1 µL Helium @ 55 cm/sec (constant flow) 235 °C to 320 °C @ 25 °C/min MSD @ 230 °C Analytes at 24 ppm in BSTFA 1. Benzoylecgonine-d3 2. Benzoylecgonine

O

N

O

O

N

Cocaine

O

O O 2.5

2.6

2.7

2.8

2.9

3.0

3.1

3.2

3.3

3.4

3.5

3.6

3.7

3.8

3.9

4.0

min

Benzoylecgonine on a Zebron™ ZB-5MSi Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample: 1,2

17792

2.8

3.0

3.2

3.4

3.6

3.8

4.0

4.2

O

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1 @ 250 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 160 °C to 320 °C @ 20 °C/min MSD @ 320 °C Analytes at 24 ppm in BSTFA 1. Benzoylecgonine-d3 2. Benzoylecgonine

4.4

4.6

4.8

5.0

5.2

5.4 min

Please inquire about other applications.

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Applications

Cocaine

App ID 17792

O

p. 13

Amphetamines

Amphetamines

Extraction of Amphetamine from Urine and Analysis by LC/MS or GC/MS Amphetamines are very addictive stimulant drugs that affect the central nervous system. Methamphetamines are metabolized to amphetamines so abuse is typically detected by monitoring both compounds initially by immunoassay. A positive result is then confirmed by LC/MS or GC/MS.

Solutions Premier Solution: Strata -X-C Low Cost Solution: Strata® Screen-C ™

SPE

Amphetamines are polar aromatic compounds that are basic in nature. This polarity makes them difficult to retain through hydrophobic interactions, but they are well-suited for cation-exchange sorbents such as Strata Screen-C or Strata-X-C. High organic washes remove a majority of hydrophobic matrix interferences that can build up on the GC columns.

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

GC/MS

When compared to other drug classes, these compounds are more volatile and require a lower starting oven condition to achieve proper peak shape. However, by ramping quickly up to 320 oC matrix interferences can still be efficiently removed preventing system stability problems. It is also worth noting that separation is achieved between the analyte and its deuterated analog. This separation does not affect quantitation in any way.

Premier Solution: Gemini® C18

LC/MS

At high pH, basic compounds, such as amphetamines and methamphetamines, are neutralized. Gemini is pH stable from 1-12, providing longer column lifetime when running at high pH.

Absolute Recovery of Amphetamines by SPE Amphetamine

100

80

83

86

76

70 60

NH2

70

68 66

65

50

0 SPE Method Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Wash 3: Elution: Evaporate: Reconstitute: Note:

Sample

Methamphetamines

MDA-DS

MDA

MDMA

HMMA

20

MDMA-DS

30

Amphetamines

40

10

Methamphetamine

NH

90

% Recovery

Applications

p. 14

Preparation Tip

Strata-X-C

Before

Strata-X-C, 30 mg/1 mL 8B-S029-TAK 1 mL methanol followed by 1 mL water 2 mL porcine plasma spiked with analytes diluted 1:2 with water 1 mL water 1 mL 0.1 M acetic acid 1 mL methanol 1 mL 2 % of ammonium hydroxide (28-30 %)* in methanol (2 x 500 µL) To dryness under gentle nitrogen flow Add 200 µL mobile phase To prevent the loss of amphetamine and methamphetamine during solvent evaporation, add two drops of concencentrated HCl before the evaporation step

blowing down samples, be sure to make them into the salt form by acidifying your solution.Failure to do so can result in sample loss due to evaporation and resulting problems with sample

*Most commercially available NH4OH is 28-30 % stock concentration

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Amphetamines GC/MS Cut off 500 ng/mL Derivatization HFBA Mass Ion(s)

Quant Ion

Secondary Ion

Tertiary Ion

D11-Amphetamine (IS)

244

128

 

Amphetamine

240

118

117

D14-Methamphetamine (IS)

261

213

Methamphetamine

254

210

118

D5-MDA (IS)

167

380

244

MDA

162

375

240

D5-MDMA (IS)

258

213

164

MDMA

254

210

389

D5-MDEA (IS)

273

241

408

MDEA

268

240

403

Amphetamines in Urine on a Gemini C18 Column: Dimensions: Part No.: Mobile Phase: Flow Rate: Detection:

17900 1

Gemini 5 µm C18 150 x 3.0 mm 00F-4435-Y0 10 mM Ammonium Formate, 0.1 % formic acid / Acetonitrile (90:10) 0.6 mL/min Sample: MS @ 450 °C 1. 4-Hydroxy-3-methoxymethamphetmine (HMMA) 2. Amphetamine (AMPH) 3. Methamphetamine (M-AMPH) 4. 3,4-Methylenedioxy-amphetamine (MDA)-d5 5. 3,4-Methylenedioxy-amphetamine (MDA) 6. 3,4-Methylendiox-methamphetamine (MDMA)-d5 7. 3,4-Methylendiox-methamphetamine (MDMA)

Amphetamines on a Zebron™ ZB-Drug-1 Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

App ID 17807

App ID 17900

®

3 6 2

17807 5 7

4 0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

7.0

7.5

min

4 3

Expanded Amphetamines on a Zebron™ ZB-Drug-1 App ID 17809

0.0

1 2

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 260 °C, 1 µL Helium @ 55 cm/sec (constant flow) 110 °C to 320 °C @ 25 °C/min MSD @ 320 °C Analytes are 10 ppm derivatized with HFBA 1. Amphetamine-d11 2. Amphetamine 3. Methamphetamine-d14 4. Methamphetamine

17809

2

4 3

1

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 280 °C, 0.1 µL Helium @ 55 cm/sec (constant flow) 70 °C to 130 °C @ 20 °C/min to 270 ºC @ 30 ºC MSD @ 230 °C Analytes are 40 ppm derivatized with HFBA 1. Amphetamine-d11 2. Amphetamine 3. Methamphetamine-d14 8 4. Methamphetamine 5. MDA-d5 7 6. MDA 7. MDMA-d5 8. MDMA 9. MDEA-d5 6 10. MDEA

NH 2

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

2.6

2.8

3.0

3.2

3.4

3.6

3.8

4.0

4.2

4.4

4.6 min

5

9

10

3

4

5

6

min

Please inquire about other applications.

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Applications

p. 15

Opiates

Opiates Extraction of Opiates from Biological Matrices with High Recovery and Analysis by LC/MS or GC/MS There are a number of opiate related compounds known to have addictive properties. The standard structure contains five ring systems and is substituted at various positions with hydroxyls, ketones, or other groups that contribute to both its pain reducing and its addictive properties.

Solutions Premier Solution: Strata -X-C Low Cost Solution: Strata® or Strata Screen-C ™

SPE

GC/MS

The nitrogen containing ring system give opiates their basic property and allows them to be extracted using mixed-mode cation exchange sorbents. The ion-exchange retention mechanism allows for much more aggressive wash conditions (up to 100 % organic) for more effective removal of other co-extracted urine components.

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

If a person has been using other opiate related compounds such as heroin or norcodeine, these compounds will also be co-extracted and can interfere with the analysis. In such cases, alternative GC/MS conditions should be developed to allow for accurate quantitation of these compounds.

O

Premier Solution: Luna® HILIC

LC/MS

A typical SPE method elutes opiates in a high concentration of organic solvent. This concentration is stronger than what is typically used to begin a reversed phase LC method, thus requiring an additional dry-down step followed by reconstitution in the proper mobile phase concentrations before injection. Employing HILIC (Hydrophilic-Interaction-Liquid-Chromatography) is an excellent complement to this extraction process, since this technique uses a starting mobile phase which employs a high concentration of organic solvent. Luna HILIC columns are ideally suited for the direct injection of eluted samples following SPE.

Standard Solution: Synergi™  Hydro-RP

Synergi Hyrdro-RP (C18) has an enhanced hydrophobic selectivity that provides extra retention for polar drugs. This results in baseline separation for all opiates. The column is also compatible with 100 % aqueous conditions. GC/MS Cut off Derivatization

Recovery of Opiates by SPE 100 90

300 ng/mL BSTFA (with 1 % TMCS)

Mass Ion(s)

101

Quant Ion

Secondary Ion

Tertiary Ion

D6 Codeine (IS)

377

349

 

80

Codeine

371

343

234

70

D3 Morphine (IS)

432

417

60

Morphine

429

414

50 40 30 20 10

401

Opiates on a Zebron™ ZB-Drug-1

O

App ID 17806

% Recovery

H

17806

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

3,4

2

0 Strata-X-C

SPE Method Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Elute: Evaporate: Reconstitute:

1

Strata-X-C, 30 mg/1mL 8B-S029-TAK 1 mL methanol followed by 1 mL water 1 mL urine spiked with analytes and IS (0.8 µg/mL) Dilute 1:1 with phosphate buffer solution (pH 7) Acidified with 2 % phosphoric acid 1 mL 0.1 N HCI 1 mL methanol 1 mL 5 % NH4OH/Methanol (2 x 500 µL) Dry down under nitrogen Add 200 µL water

4.0

4.1

4.2

4.3

4.4

4.5

4.6

4.7

Sample: 1. Morphine 2. Normorphine 3. Morphine-3-ß-glucuronide

Column: Dimensions: Part No.: Mobile Phase:

3

1

Looking

to run opiates on a standard C18 column? Please inquire with your local Phenomenex representative.

Gradient: Flow Rate: Detection: Note:

2

OH

0

Web:

4.8

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 280 °C, 1 µL Helium @ 55 cm/sec (constant flow) 180 °C to 340 °C @ 20 °C/min MSD @ 230 °C Analytes are 24 ppm derivatized with BSTFA 1. Codeine-d6 2. Codeine 3. Morphine-d3 4. Morphine min

Morphine and Metabolites on a Luna HILIC 16445

App ID 16445

Applications

p. 16

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1

2

I Email:

3

4

5

6

international@phenomenex .com

N

Luna 3 µm HILIC 100 x 2.0 mm 00D-4449-B0 A: Acetonitrile / 100 mM Ammonium formate, pH 3.2 (90:10) B: Acetonitrile / 100 mM Ammonium formate, pH 3.2 (50:50) A/B (100:0) for 1 min to A/B (0:100) in 2 min, hold for 1.5 min 0.4 mL/min MS Direct injection following Acetonitrile/ Water (5:1) protein precipitation (PPT) 7

8

9

min

Healthcare Opiates

Healthcare Opiates Extraction of Various Prescribed Opiates from Urine and Analysis by GC/MS Even though opiate related compounds pose the risk of addiction, their therapeutic benefit is important for pain management. Opiates such as hydrocodone (Vicodin), oxycodone (OxyContin), and meperidine (Demerol) are routinely prescribed for pain control, but due to their addictive qualities, they often become the subject of abuse.

Solutions Premier Solution: Strata -X-CW Low Cost Solution: Strata® Screen-C ™

SPE

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

To improve peak shape, opiates are often derivatized using BSTFA with 1 % TMCS. When analyzing one or two opiates, this approach works well. However, some opiate structures form multiple products when single step derivatization is done. To ensure a single reaction product for hydromorphone or oxycodone, an oxime derivative is formed prior to reaction with BSTFA. The oxime reaction eliminates the multiple points of interaction resulting in a single derivative product. Once derivatization has been completed, GC/MS analysis can be performed. Separation of all closely related isomers can be somewhat challenging, requiring special attention to those compounds that share the same mass ions such as Norcodeine and Morphine.

GC/MS

GC/MS Cut off Derivatization

O

300 ng/mL BSTFA (with 1 % TMCS)

Mass Ion(s)

Quant Ion

Secondary Ion

Tertiary Ion

D3-Morphine (IS)

432

417

Morphine

429

414

D6-Codeine (IS)

377

349

Codeine

371

343

234

Oxymorphone

532

517

287

D6-Hydromorphone (IS)

361

450

Hydromorphone

355

444

D6-Oxycodone (IS)

480

465

Oxycodone

474

459

460

Hydrocodone

386

297

371

401 Codeine

O

429

O

O

H

H

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

Underivatized 16555

4

Zebron ZB-5MSi 30 meter x 0.25 mm x 0.25 µm 7HG-G018-11 Splitless 20:1 @ 250 °C, 1 µL Helium @ 1.5 mL/min (constant flow) 170 °C for 0.5 min to 360 °C @ 18 °C/min MSD @ 180 °C Analytes at 1 ppm in Methanol 1. Codeine 2. Norcodeine 3. Morphine 4. Hydrocodone 5. Hydromorphone 6. Oxycodone 7 7. Oxymorphone

OH

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17899

1

7 10

6

2 5 3

9

1,2

7.0

7.2

7.4

7.6

7.8

8.0

N

Expanded Opiates on a Zebron™ ZB-Drug-1 App ID 17899

App ID 16555

Healthcare Opiates on a Zebron™ ZB-5MSi

min 6

4 3 5

OH

3.9

4.0

4.1

4.2

4.3

4.4

4.5

8

4.6

4.7

4.8

4.9

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 260 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 180 °C to 340 °C @ 20 °C/min MSD @ 320 °C Analytes are 24 ppm derivatized with 1. Hydroxylamine 2. BSTFA 1. Morphine-d3 2. Morphine 3. Codeine-d6 4. Codeine 5. Oxymorphone 6. Hydromorphone-d6 7. Hydromorphone 8. Oxycodone-d6 9. Oxycodone 10. Hydrocodone

5.0

5.1

5.2

5.3

5.4

min

Please inquire about other applications.

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Applications

p. 17

Applications

p. 18

Steroids

Steroids

Extraction Steroids from Urine and/or Plasma and Analysis by LC, LC/MS or GC/MS Corticosteroids are an important class of compounds that have uses as therapeutic agents as well as some illicit uses. However, testing for them has become more challenging due to the use of drug-masking agents by athletes in an attempt to cover their steroid use. Such interferences make chemical and ELISA-based tests sometimes impractical for

accurate detection. The combination of mass spectroscopy, improved chromatography separations and efficient sample preparation by SPE allows for a specific, sensitive and accurate method for detection of steroid usage.

Solutions Premier Solution: Strata -X ™

SPE

While standards can be directly analyzed on HPLC without any difficulty, interferences due to proteins, lipids, and salts require cleanup by SPE prior to injection for plasma and urine samples. SPE using Strata-X removes most matrix contaminants from the sample that would interfere with UV and MS detection, as well as allows for concentration of dilute samples. When compared to the commonly used neutral phase SPE sorbents, such as C18, Strata-X retention allows for stronger organic washes for more optimal cleanliness and longer column lifetime.

Premier Solution: Gemini® C18

LC/MS

Most steroids are very similar in structure, as well as in molecular weight. Therefore, the need for an HPLC column that can achieve baseline resolution of steroids is critical for accurate quantitation by HPLC-UV. Gemini C18 columns can provide the hydrophobic selectivity needed for most major corticosteroids down to levels around 50 ng/mL with good linearity (r2 > 0.995) and accuracy (98 % - 105 %). While UV detection may be sensitive enough to detect current steroid usage and monitor steroid levels for therapeutic treatments, detection of past steroid usage may require the more sensitive detection offered by a tandem mass spectrometry. The sensitivity of using MS/MS detection allows quantification of background endogenous steroids (cortisone and hydrocortisone) found in blank urine. With MS detection, such low level endogenous amounts must be taken into account with any testing method for illicit steroid use. Linear quantification data using MS detection with Gemini columns deliver excellent linearity and reproducibility for such methods, and are powerful tools for quantitation of steroid levels in plasma and urine. Gemini C18 columns excel in volatile MS-friendly buffers making them the ideal choice for these methods.

HO

SPE Method Sorbent: Part No.: Condition: Load: Wash:

Strata-X, 30 mg/1 mL 8B-S100-TAK 1 mL methanol followed by 1 mL water 1 mL spiked human serum or urine (diluted 1:1 with water) 1 mL water followed by 1 mL 40 % methanol in water (up to 50-60 %)* Elute: 1 mL methanol (2 x 500 µL) Evaporate: Under nitrogen stream (ambient) Reconstitute: Add 100 μL acetonitrile/water 40:60

O

*Please see TN-0017, "Testosterone on Strata-X vs. Strata-X C18-E"

H

Prednisolone

OH

HO

O

OH H

H H

H

H

H

O

O

O Web:

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O

O

OH

HO

Cortisone

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H

H

Steroids

Steroids in Plasma-LC-UV

Applications

p. 19

Steroids in Plasma - LC-UV 1000

Conditions for All LC Steroid Methods: Column: Gemini® 5 µm C18 Dimensions: 150 x 3.0 mm Part No.: 00F-4435-Y0 Mobile Phase: A: 0.1 % Formic Acid in water B: 0.1 % Formic Acid in 95 % Acetonitrile / water Gradient: A/B (60:40) for 4 min to A/B (25:75) in 11 min Flow Rate: 0.6 mL/min Detection: LC-UV @ 254 nm or MS/MS Temperature: Ambient

Triamcinolone R2 = 0.9994

900

Prednisolone R2 = 0.9988

800

Corticosterone R2 = 0.9992 Triamcinolone acetonide R2 = 0.9991

Intensity

700

11α-Hydroxyprogesterone R2 = 0.9991

600 500 400

LC/MS/MS Chromatogram of Steroid Standard in Urine

300

App ID 17971

200 100 0 0

200

400

600

800

1000

1200

Conc.(ng/mL)

Steroids in Urine - Standard Calibration Curves LC\MS\MS

O

2.9e4

Steroids in Urine - Standard Calibration Curves LC\MS\MS (1) Intensity . cps

2.0e4

11α-Hydroxyprogesterone R2 = 0.9999 Deoxycorticosterone R2 = 1.0000 11-Ketoprogesterone R2 = 0.9999 Corticosterone R2 = 0.9999 Hydrocortisone R2 = 0.9999 Prednisone R2 = 0.9993

9 8 7

0.0

6

Intensity

OH

5

10

min

15

5 4 3

LC/MS/MS Chromatogram of a Blank Urine Sample

2

App ID 17972

1 0 0

50

100

150

200

250

300

350

400

450

Conc.(ng/mL)

Extracted ion chromatogram of different MS/MS steroids from urine blank

1.8e4

Steroids in Urine - Standard Calibration Curves LC\MS\MS Prednisolone R = 0.9998 Cortisone R2 = 0.9999 Cortisone acetate R2 = 0.9999 Triamcinolone acetate R2 = 0.9993 Betamethasone-17-valerate R2 = 0.9997 Triamcinolone R2 = 0.9997 2

16

Intensity

14

1.4e4 Intensity . cps

Steroids in Urine - Standard Calibration Curves LC\MS\MS

1.6e4

1.2e4

Cortisone (endogenous)

1.0e4 8000.0 6000.0 4000.0

12

Hydrocortisone

2000.0

10

0.0

8

2

4

6

8

10

12

14

16

18 min

6 4 2 0 0

50

100

150

200

250

300

350

400

450

Please inquire about other applications.

Conc.(ng/mL)

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Benzodiazepines

O

Benzodiazepines Extraction of Benzodiazepines from Urine or Plasma and Analysis by LC/MS or GC/MS

Benzodiazepines (benzo’s) are psychoactive drugs that are prescribed for anti-anxiety. Due to their addictive nature, benzo’s are highly regulated in most countries. Their possession can be a criminal offense if proper prescriptive information is not demonstrated.

N

Solutions SPE

Premier Solution: Strata™-X and Strata™-X-CW Low Cost Solution: Strata® Screen-C

There are a number of benzodiazepine related compounds and extraction efficiency depends on the set of compounds being analyzed. General methods can rely primarily on hydrophobic interactions using polymeric sorbents such as Strata-X. This sorbent is suitable for several different classes of compounds with recoveries between 70-130 %. Mixed-mode weak ion-exchangers such as Strata-X-CW can also be used to achieve high recoveries for certain benzo’s with high pKa values.   Improved Solution: Luna® C8(2) and Synergi™ Fusion-RP

LC/MS

Benzo’s are well-suited to analysis by reversed phase HPLC analysis without derivatization. Eliminating this time consuming step can significantly improve sample throughput. High speed LC/MS analysis can be done using the Luna C8(2) or Synergi Fusion cartridges. These 2.5 µm materials provide high column efficiency allowing for complete analysis of eight benzo’s in under 2 minutes.

GC/MS

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

SPE Method: Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Dry: Elute: Evaporate: Reconstitute:

Benzodiazepines on a Synergi Fusion-RP App ID 16042

Recovery of Benzodiazepines by SPE Strata-X-C, 60 mg/3 mL 8B-S029-UBJ 2 mL methanol followed by 2 mL water 2 mL spiked with analytes and IS (0.8 µg/mL); dilute 1:1 with 100 mM KH2PO4 (pH 6); sample acidified with 2 % H3PO4 2 mL 0.1 N HCl 2 mL methanol 10 min under vacuum 2 mL 5 % NH4OH/methanol (2 x 1 mL) Dry down under slow stream of nitrogen Add 200 µL mobile phase

Column: Dimensions: Part No.: Mobile Phase:

16042 6 1.00e6 9.00e5

Gradient:

8.00e5

Flow Rate: Detection: Injection:

3

7.00e5 6.00e5

Sample:

5.00e5

Wash 1: Wash 2: Dry: Elute: Evaporate: Reconstitute:

4.00e5

Strata-X-CW, 30 mg/1 mL 8B-S035-TAK 1 mL methanol followed by 1 mL D.I. water 1 mL analyte spiked human urine (dilute 500 µL urine with 25 mM sodium acetate, pH 4.5 (1:1) 1 mL 25 mM sodium acetate, pH 4.5 1 mL methanol/acetonitrile/ 2 % acetic acid (1:1:8) 2 min at 10 inches of Hg 1 mL Ethylacetate. Add 50 µL of Flurazepam (20.0 μg/mL) (2 x 500 µL) Under slow stream of nitrogen to dryness (no heat) Add 100 µL 15 % acetonitrile in water

3.00e5

CI

0.00

96

100

102 105

98

93

2 0

2 min

1

Benzodiazepines on a Luna® C8(2)

96

16040 6 1.10e6 1.00e6

Column: Dimensions: Part No.: Mobile Phase: Gradient:

9.00e5

Flow Rate: Detection: Sample:

Flunitrazepam

Diazapam

Flurazepam

Bromazepam

Temazepam

Lorazepam

Oxazepam

8.00e5

Diazepam

20 10 0

97

2 3

7.00e5 6.00e5 5.00e5

7 4.00e5 1 3.00e5

Strata-X-CW

Synergi 2.5 µm Fusion-RP MercuryMS™ Cartridge 10 x 2.0 mm 00N-4423-B0-CE A: 0.1 % Formic Acid in Water B: 0.1 % Formic Acid in Acetonitrile 85:15 (A/B) to 5:95 in 1 min, re-equilibrate to 85:15 in 1 min 0.6 mL/min MS 10 µL of 100 ng/mL Benzodiazepines in urine after SPE cleanup 1. 7-Aminoflunitrazepam 2. Chlordiazepoxide 3. Oxazepam 4. Nordazepam 5. Clonazepam (IS) 6. Temazepam 7. Flunitrazepam 8. Diazepam

Note: Holder required. See page 32 for ordering information.

94

Nordazepam

80 70 60 50 40 30

94

2

1.00e5

110 100 90

1

2.00e5

App ID 16040

SPE Method: Sorbent: Part No.: Condition: Load:

% Recovery

Applications

p. 20

Strata-X-C

Luna 3 µm C8(2) MercuryMS™ Cartridge 20 x 2.0 mm 00M-4248-B0-CE A: 0.1 % Formic Acid in Water B: 0.1 % Formic Acid in Acetonitrile A/B (85:15) to A/B (5:95) in 1 min to (85:15) hold for 1 min 0.6 mL/min MS 1. 7-Aminoflunitrazepam 2. Chlordiazepoxide 3. Oxazepam 4. Nordazepam 5. Clonazepam (IS) 6. Temazepam 7. Flunitrazepam 8. Diazepam

2.00e5 1.00e5 0.00

0.5

1.0

1.5

2.0 min

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CI

N

Benzodiazepines

O

Bromazepam

O

O N

NH

N

N

Flunitrazepam

Flurazepam

Br

O

CI N

N

O

N N

N O

N

N+

F

CI

F

O

Mass Ion(s)

Quant Ion

Secondary Ion

D5-Nordiazepam-TBDMS (IS)

332

334

 

Nordiazepam-TBDMS

327

328

383

462

519

457

513

459

362

288

390

357

359

385

491

513

493 326

D5-Oxazepam-TBDMS (IS) Oxazepam-TBDMS Serax

®

D5-Temazepam-TBDMS (IS) Temazepam-TBDMS Restoril® Lorazepam-TBDMS Ativan®

O

Benzodiazepines on a Zebron™ ZB-Drug-1

100 ng/mL MTBSTFA* (with 1 % MTBDMCS)

N

Tertiary Ion

App ID 17808

GC/MS Cut off Derivatization

Sample: Analytes are 24 ppm derivatized with MTBSTFA 1. Diazepam 2. Nordiazepam-d5 3. Nordiazepam 4. Oxazepam-d5 5. Oxazepam 6. Temazepam-d5 7. Temazepam 8. Clonazepam 9. Lorazepam 10. Alprazolam 11. α-Hydroxyalprazolam-d5 12. α-Hydroxyalprazolam

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program:

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 20:1 @ 280 °C, 0.1 µL Helium @ 55 cm/sec (constant flow) 200 °C to 210 °C @ 20 °C/min to 320 ºC @ 30 ºC for 1 min Detection: MSD @ 320 °C

17808

Clonazepam Clonopin®

372

374

Diazepam Valium®

256

283

221

Alprazolam Xanax®

279

308

204

D5-α-Hydroxyalprazolam-TBDMS (IS)

386

388

α-Hydroxyalprazolam-TBDMS

381

383

2,3

1

4,5

6,7

9

11,12 8 2.2

384

2.4

2.6

2.8

3

3.2

3.4

10 3.6

3.8

4

4.2

4.4

4.6

4.8

5 min

Benzodiazepines on a Zebron™ ZB-5MSi App ID 17894

N

Diazepam

Column: Dimensions: Part No.: Injection: Carrier Gas:

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1 @ 260 °C, 1 µL Helium @ 1 mL/min (constant flow) Oven Program: 200 °C to 340 °C @ 25 °C/min for 1 min Detection: MSD @ 320 °C 17894 2

3

N

Sample: Analytes are 24 ppm derivatized with MBSTFA 1. Diazepam 7. Temazepam 2. Nordiazepam-d5 8. Clonazepam 3. Nordiazepam 9. Lorazepam 4. Oxazepam-d5 10. Alprazolam 5. Oxazepam 11. α-Hydroxyalprazolam-d5 6. Temazepam-d5 12. α-Hydroxyalprazolam 11

12

6, 7 4, 5 9 8

1

2.0

2.2

2.4

2.6

2.8

3.0

3.2

3.4

10

3.6

3.8

4.0

4.2

4.4

4.6 min

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p. 21

Barbiturates

Barbiturates

Extraction of Barbiturates from Urine or Plasma and Analysis by LC, LC/MS or GC/MS Barbiturates are depressants that are typically classified into ultrashort-, short-, intermediate-, and long-acting varieties. When abused, they cause feelings similar to intoxication and induce a “mellow” or relaxed state.

Solutions Premier Solution: Strata™-X-C Low Cost Solution: Strata® Screen-C

SPE

Barbiturates are weakly acidic in nature but can be retained through hydrophobic and hydrogen bonding interactions in SPE. Washing with acidified organic allows for removal of matrix contamination.   Premier Solution: Synergi™ Max-RP

HPLC/MS

GC/MS

Barbiturates can be analyzed by HPLC without derivatization using reversed phase LC, such as with the Synergi Max-RP C12 column. The Synergi Max-RP C12 column is also available in the 2.5 μm HST (High Speed Technology) format. These highly efficient columns allow for rapid LC/MS analysis.

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi

Analysis by GC/MS requires derivatization to improve quantitation. Derivatization with trimethylanilinium hydroxide (TMPAH) allowed five controlled barbiturates to be separated in less than 5 minutes.

Recovery of Barbiturates by SPE Butalbital

120

O

NH

O

110 100

100 107 102

90

NH

101 103 100 O

80 70 60

Amobarbital

50 O

Amobarbital

Butalbital

10

Phenobarbital

20

Amobarbital

30

Butalbital

40 Phenobarbital

% Recovery

Applications

p. 22

NH

O

NH

O

0 Strata Screen-C

Strata-X-C

SPE Method: Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Dry: Elute: Evaporate: Reconstitute:

Strata-X-C, 60 mg/1 mL 8B-S029-UBJ 2 mL methanol followed by 2 mL water 2 mL analyte and IS spiked human urine (dilute 1:1 with water) 2 mL 5 % methanol in water 2 mL 10 % methanol / 2 % acetic acid 1 min @ 10 in. of Hg 2 mL 35 % methanol / 2 % ammonium hydroxide At 40 ºC under slow stream of N2 Add 300 µL mobile phase

SPE Method Sorbent: Part No.: Condition: Load: Wash 1: Wash 2: Elute: Evaporate: Reconstitute:

Strata Screen-C, 150 mg/6 mL 8B-S016-SCH 2 mL methanol followed by 2 mL water 2 mL human urine spiked with analytes and IS (0.5 µg/mL); dilute 1:1 with water 2 mL 5 % methanol in water 2 mL 10 % methanol / 2 % acetic acid, Dry 1 min under vacuum 2 mL methanol Dry down @ 40 ºC under slow stream of nitrogen Add 300 µL mobile phase buffer

Phenobarbital

O

NH

O

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O

NH

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Barbiturates

O

GC/MS Cut off Derivatization

200 ng/mL TMPAH (optional) Quant Ion

Secondary Ion

D5-Butalbital (IS)

Mass Ion(s)

201

184

214

Butalbital

196

181

138

Amobarbital

169

184

225

Pentobarbital

169

184

225

Secobarbital

196

181

138

D5-Phenobarbital (IS)

122

151

233

Phenobarbital

232

146

117

NH

Tertiary Ion

App ID 17738

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17738

4 3

2

Barbiturates on a Zebron™ ZB-Drug-1

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1 @ 260 °C, 1 µL Helium @ 0.6 mL/min (constant flow) 100 °C to 320 °C @ 20 °C/min for 5 min MSD @ 230 °C Analytes are 12 ppm derivatized with TMPAH 1. Butalbital-d5 2. Butalbital 3. Amobarbital 7 4. Pentobarbital 5. Secobarbital 6. Phenobarbital-d5 6 7. Phenobarbital

App ID 17810

Barbiturates on a Zebron™ ZB-5MSi

17810 4

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

3

5

1, 2

5

1

3.2

3.0

3.6

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 260 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 145 °C to 300 °C @ 25 °C/min MSD @ 230 °C Analytes are 12 ppm derivatized with TMPAH 1. Butalbital-d5 2. Butalbital 3. Amobarbital 4. Pentobarbital 5. Secobarbital 7 6. Phenobarbital-d5 7. Phenobarbital

NH 6

3.8

4.0

4.2

4.4

4.6

4.8

5.0

min

1.4

1.6

1.8

2.0

2.2

2.4

2.6

2.8

3.0

3.2

min

App ID 14622

™ Barbiturates on a Synergi  Max-RP

Column: Dimensions: Part No.: Mobile Phase:

14622

Gradient: Flow Rate: Detection: Sample:

1

2

4

3

Synergi 4 µm Max-RP 150 x 4.6 mm 00F-4337-E0 A: 20 mM KH2PO4, pH 7.0 B: Acetonitrile A/B (80:20) hold 3 min to A/B (50:50) in 12 min to A/B (80:20) in 1 min 1 mL/min UV @ 210 nm 1. Phenobarbital 2. Butabarbital 3. Butalbital 4. Amobarbital 5. Secobarbital

O

5

2

4

6

8

10

12

14 min

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p. 23

6-MAM

6-MAM

Extraction of 6-Monoacetylmorphine (6-MAM) from Urine and Analysis by GC/MS 6-Monoacetylmorphine is a unique metabolite of heroin and can be used to differentiate between users of heroin and other legal and illegal opiate drugs.

Solutions Premier Solution: Strata™ -X or X-CW Low Cost Solution: Strata® Screen-C

Extraction of 6-MAM is similar to other opiate related species. A mixed-mode cation-exchange sorbent such as Screen-C or Strata-X-C ionically retains 6-MAM, and wash solutions with up to 100 % methanol are used to remove weakly bound matrix interferences. However, the use of ammonium hydroxide for elution can make the acetate group in 6-MAM unstable, converting it into morphine. To avoid any potential problems, use a weak cation exchanger such as Strata-X-CW or a neutral sorbent such as Strata-X. Strata-X-CW sorbent can be neutralized by eluting with 2 % formic acid in methanol or any other oganic solvent.

SPE

Premier Solution: Zebron™ ZB-Drug-1 Standard Solution: Zebron™ ZB-5MSi GC/MS

Heroin can be metabolized into 6-Monoacetylmorphine (6-MAM) and 3-Monoacetylmorphine. 6-MAM can be further metabolized into morphine, so is often found in samples as well. By scanning simply for the 6-MAM ions, high sensitivity confirmation of heroin abuse can be determined. If you wish to detect other opiates as well, simply adjust the SIM ion program to include ions for those analytes. Analysis of all three analytes is possible using the same GC/MS column. GC/MS Cut off Derivatization

10 ng/mL BSTFA (with 1 % TMCS)

6-MAM

Quant Ion

Secondary Ion

Tertiary Ion

6-Monoacetylmorphine (6-MAM)

399

400

340

D5-6-Monoacetylmorphine (6-MAM d5) (IS)

402

403

Mass Ion(s)

OH Morphine

HO

O

N

6-MAM on a Zebron™ ZB-5MSi

O

O

App ID 17789

O

H

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17789

N

1,2

HO

Zebron ZB-5MSi 10 meter x 0.18 mm x 0.18 µm 7CD-G018-08 Split 10:1 @ 280 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 170 °C to 300 °C @ 20 °C/min MSD @ 320 °C Analytes are 1 ppm derivatized with BSTFA 1. 6-MAM-d3 2. 6-MAM

Recovery of 6-MAM by SPE 120

100

99

102

3.6

80

3.8

4.0

4.2

4.4

4.6

60

40

17802

1,2

20

0

4.8

5.0

5.2

5.4

min

6-MAM on Zebron™ ZB-Drug-1 App ID 17802

% Recovery

Applications

p. 24

Strata-X-CW

SPE Method: Sorbent: Part No.: Condition: Load: Wash: Elute: Dry: Evaporate: Reconstitute:

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

Zebron ZB-Drug-1 10 meter x 0.18 mm x 0.18 µm 7CD-G023-08 Split 10:1 @ 260 °C, 1 µL Helium @ 0.7 mL/min (constant flow) 245 °C to 300 °C @ 25 °C/min MSD @ 300 °C Analytes are 1 ppm derivatized with BSTFA 1. 6-MAM-d3 2. 6-MAM

Strata-X

Strata-X, 60 mg/ 3 mL 8B-S100-UBJ 2 mL methanol followed by 2 mL water 2 mL spiked human urine (diluted 1:2 with water) 2 mL water followed by 2 mL 10 % methanol/water 2 mL methanol 2 min at 10 in. Hg Blow down to dryness under nitrogen at 40 °C Add 0.50 µL ethylacetate and 50 µL BSTFA (1 % TMCS). React at 70 °C for 20 min.

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2.2

2.4

2.6

2.8

3.0

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3.2

3.4

3.6

3.8

4.0

4.2

min

Blood Alcohols

Blood Alcohols

Fast and Accurate Blood Alcohol Analysis by GC/FID Blood alcohol analysis is typically used by law enforcement to determine if a driver was unlawfully operating a vehicle. The results will be used in a court of law, therefore it is important to minimize systematic and operator errors.

Solutions Premier Solution: Zebron ZB-BAC1 and 2 ™

GC/MS

The new Zebron ZB-BAC1 and 2 column pair is optimized for fast ethanol separation. These columns resolve both t-Butanol and n-Propanol from other volatile solvents, enabling the use of either compound as the internal standard. This is advantageous because n-Propanol, which may be present in blood samples as a result of bacterial growth, is not a reliable standard in some cases. Zebron ZB-BAC1 and 2 are ideal for law enforcement applications where breathalyzer tests are not feasible as well as with post mortem samples.

Confirmation of Blood Alcohols on a Zebron™ ZB-BAC2

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17897

5

4

2

3

6

Zebron ZB-BAC1 30 meter x 0.32 mm x 1.80 µm 7HM-G021-31 Split 5:1 @ 140 °C, 0.5 mL Helium @ 80 cm/sec (constant flow) 40 °C (Isothermal) FID @ 220 °C 1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. t-Butanol (IS) 7. 2-Butanol (IS)

App ID 17898

App ID 17897

Blood Alcohols on a Zebron™ ZB-BAC1

Column: Dimensions: Part No.: Injection: Carrier Gas: Oven Program: Detection: Sample:

17898

5

2 4 3 6 1

Zebron ZB-BAC2 30 meter x 0.32 mm x 1.20 µm 7HM-G022-25 Split 5:1 @ 140 °C, 0.5 mL Helium @ 80 cm/sec (constant flow) 40 °C (Isothermal) FID @ 220 °C 1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. t-Butanol (IS) 7. 2-Butanol (IS)

7

1

7

0

1

2

3

min

0

1

2

3

min

GC Tip When

working with post mortem samples, it is often safer to use t-Butanol as your internal standard. N-Propanol, a common internal standard, might be present as a result of bacterial growth and can give you misleading results. Since the Zebron ZB-BAC1 and 2 GC columns completely resolve t-Butanol, you can use it as an internal standard and attain more accurate results.

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Complete Support • Technical specialists available to help with your troubleshooting and method optimization • Full range of method development guides and reference tools • Complete technical solutions for sample preparation and HPLC/GC analysis

Global Assistance Phenomenex technical specialists are available to provide technical and product support worldwide.

Method Development FREE application and troubleshooting services are available to help you create optimized SPE methods for your analysis.

Reference Tools Comprehensive guides and technical notes are available to meet all your SPE, GC or LC needs.

Please ask your Phenomenex technical consultant for complimentary methods on • Extraction of Amphetamines from Urine using Strata® Screen-C (ER-0001) • Simplified Method Development for the Extraction of Acidic, Basic, and Neutral Drugs with a Single SPE Sorbent Strata™-X (TN-0004) • Sample Preparation of Tricyclic Antidepressants for Drug Screens: The Advantages of Strata-X over Mixed-Mode Sorbents (TN-0006) • LC/MS/MS Analysis of Basic Drugs with Positive Ion Electrospray in High pH Mobile Phase (TN-1031) Please inquire about other applications from our Application and Technote Library.

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Ordering Information

Ordering

information

p. 28

Sample Prep./SPE

Strata® and Strata™-X SPE Sorbents Strata™-X Format Tube

Strata® Screen-A

Sorbent Mass

Part. No.

30 mg 30 mg 60 mg 200 mg 100 mg 200 mg 500 mg

8B-S100-TAK 8B-S100-TBJ 8B-S100-UBJ 8B-S100-FBJ 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH

Unit

Format Tube

Price

1 mL (100/box)* 3 mL (50/box) 3 mL (50/box)** 3 mL (50/box) 6 mL (30/box) 6 mL (30/box) 6 mL (30/box)

Format Tube

10 mg 8E-S100-AGB 2 Plates/Box 30 mg 8E-S100-TGB 2 Plates/Box 60 mg 8E-S100-UGB 2 Plates/Box *ASPEC capped tubes available in 500/bag (8B-S100-TAM-A) **Tab-less tubes available (8L-S100-UBJ)

Part. No.

Unit

30 mg 30 mg 60 mg 200 mg 100 mg 200 mg 500 mg

8B-S029-TAK 8B-S029-TBJ 8B-S029-UBJ 8B-S029-FBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH

1 mL (100/box) 3 mL (50/box) 3 mL (50/box) 3 mL (50/box) 6 mL (30/box) 6 mL (30/box) 6 mL (30/box)

10 mg 30 mg 60 mg

8E-S029-AGB 8E-S029-TGB 8E-S029-UGB

2 Plates/Box 2 Plates/Box 2 Plates/Box

Format Tube

Price

Part. No.

Unit

60 mg 200 mg 100 mg 200 mg 500 mg

8B-S043-UBJ 8B-S043-FBJ 8B-S043-EBJ 8B-S043-FCH 8B-S043-HCH

3 mL (50/box) 3 mL (50/box) 3 mL (50/box) 6 mL (30/box) 6 mL (30/box)

30 mg

8E-S043-TGB

2 Plates/Box

Price

96-Well Plate

Strata™-XL-C Format Tube

Sorbent Mass

Part. No.

Unit

30 mg 60 mg 200 mg 100 mg 200 mg 500 mg

8B-S044-TAK 8B-S044-UBJ 8B-S044-FBJ 8B-S044-ECH 8B-S044-FCH 8B-S044-HCH

1 mL (100/box) 3 mL (50/box) 3 mL (50/box) 6 mL (30/box) 6 mL (30/box) 6 mL (30/box)

30 mg

8E-S044-TGB

2 Plates/Box

8B-S019-FBJ 8B-S019-HCH

3 mL (50/box) 6 mL (30/box)

Price

Sorbent Mass

Part. No.

Unit

200 mg 200 mg 500 mg 500 mg 1000 mg

8B-S026-FBJ 8B-S026-FCH 8B-S026-HBJ 8B-S026-HCH 8B-S026-JCH

3 mL (50/box) 6 mL (30/box) 3 mL (50/box) 6 mL (30/box) 6 mL (30/box)

Sorbent Mass

Part Number

Unit

30 mg 60 mg 200 mg 200 mg

8B-S035-TAK 8B-S035-UBJ 8B-S035-FBJ 8B-S035-FCH

1 mL (100/box) 3 mL (50/box) 3 mL (50/box) 6 mL (30/box)

Part No. Description 24 – Position Vacuum Manifold AH0-6024 SPE 24-Position Vacuum Manifold Set, complete assembly 24 – Position Vacuum Manifold Replacement Parts AH0-6026 SPE Glass Chamber AH0-6028 SPE Cover, Gasket and 24 Stopcocks AH0-6030 SPE Gaskets AH0-6038 SPE Collection Rack, including shelves, legs, clips and posts AH0-6049 SPE Luer Stopcocks 12 – Position Vacuum Manifold AH0-6023 SPE 12-Position Vacuum Manifold Set, complete assembly 12 – Position Vacuum Manifold Replacement Parts AH0-6025 SPE 12-Position Glass Chamber AH0-6027 SPE Cover, Gasket and 12 Stopcocks AH0-6029 SPE Gaskets AH0-6037 SPE Collection Rack, including shelves, legs, clips and posts AH0-6052 SPE 12-Position Vacuum Waste Container, polypropylene AH0-6049 SPE Luer Stopcocks

Strata™-XL Format Tube

200 mg 500 mg

SPE Vacuum Manifolds

96-Well Plate

Sorbent Mass

Unit

Strata™-X-CW

Strata™-X-C Format Tube

Part. No.

Strata® Screen-C GF

96-Well Plate

Sorbent Mass

Sorbent Mass

Price

Price

Unit

Price

ea

ea ea 2/pk ea 24/pk ea

ea ea 2/pk ea 10/pk 24/pk

Strata On-Line Extraction Cartridges

Price

Part No. Description Unit/Box 00M-S033-B0-CB Strata-X on-line extraction cartridge, ea 20 x 2.0 mm 00M-S036-B0-CB Strata-X-CW on-line extraction cartridge, ea 20 x 2.0 mm 00M-S039-B0-CB Strata C18 on-line extraction cartridge, ea 20 x 2.0 mm 00M-S101-B0-CB Strata C8 on-line extraction cartridge, ea 20 x 2.0 mm CH0-5845 Cartridge holder, 20 mm ea

96-Well Plate

Price

Strata® Screen-C Format Tube

Sorbent Mass

100 mg 100 mg 150 mg 200 mg 300 mg 150 mg *200/box available (8B-S016-5BL)

Part. No.

Unit

8B-S016-EAK 8B-S016-EBJ 8B-S016-SBJ 8B-S016-FBJ 8B-S016-RBJ 8B-S016-SCH

1 mL (100/box) 3 mL (50/box) 3 mL (50/box) 3 mL (50/box) 3 mL (50/box) 6 mL (30/box)

Web:

Price

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Zebron™-High Performance GC Columns ZB-Drug-1 ID(mm) 10-Meter 0.18 15-Meter 0.25 30-Meter 0.25

Temp. Limits °C

Part No.

0.18

-60 to 320/340 °C

7CD-G023-08

0.25

-60 to 320/340 °C

7EG-G023-11

0.25

-60 to 320/340 °C

7HG-G023-11

ZB-BAC1 ID(mm) 30-Meter 0.32 0.53

df(µm)

Temp. Limits °C

Part No.

1.80 3.00

-20 to 260/280 °C -20 to 260/280 °C

7HM-G021-31 7HK-G021-36

ZB-BAC2 ID(mm) 30-Meter 0.32 0.53

df(µm)

Temp. Limits °C

Part No.

1.20 2.00

-20 to 260/280 °C -20 to 260/280 °C

7HM-G022-25 7HK-G022-32

ZB-5MSi ID(mm) 10-Meter 0.18 15-Meter 0.25 30-Meter 0.25 0.25 0.32 0.32 60-Meter 0.25

df(µm)

Temp. Limits °C

Part No.

0.18

-60 to 360/370

7CD-G018-08

0.25

-60 to 360/370

7EG-G018-11

0.25 0.50 0.25 0.50

-60 to 360/370 -60 to 360/370 -60 to 360/370 -60 to 360/370

7HG-G018-11 7HG-G018-17 7HM-G018-11 7HM-G018-17

0.25

-60 to 360/370

7KG-G018-11

df(µm)

Temp. Limits °C

Part No.

0.10 0.18

-60 to 325/350 -60 to 325/350

7CB-G010-02 7CD-G010-08

0.25

-60 to 325/350

7EG-G010-11

0.18 0.32 0.36

-60 to 325/350 -60 to 325/350 -60 to 325/350

7FD-G010-08 7FD-G010-51 7FD-G010-53

0.25 0.50 1.00 0.25 0.50 1.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

7HG-G010-11 7HG-G010-17 7HG-G010-22 7HM-G010-11 7HM-G010-17 7HM-G010-22

0.25 0.25

-60 to 325/350 -60 to 325/350

7KG-G010-11 7KM-G010-11

ZB-5ms ID(mm) 10-Meter 0.10 0.18 15-Meter 0.25 20-Meter 0.18 0.18 0.18 30-Meter 0.25 0.25 0.25 0.32 0.32 0.32 60-Meter 0.25 0.32

ZB-1ms

df(µm)

ID(mm) 10-Meter 0.10 0.18 12-Meter 0.20 15-Meter 0.25 0.32 20-Meter 0.18 25-Meter 0.20 30-Meter 0.25 0.25 0.25 0.25 0.32 0.32 60-Meter 0.25 0.25 0.32

Price

Price

Price

df(µm)

Temp. Limits °C

Part No.

0.10 0.18

-60 to 360/370 -60 to 360/370

7CB-G011-02 7CD-G011-08

0.33

-60 to 360/370

7DE-G011-14

0.25 0.25

-60 to 360/370 -60 to 360/370

7EG-G011-11 7EM-G011-11

0.18

-60 to 360/370

7FD-G011-08

0.33

-60 to 360/370

7GE-G011-14

0.10 0.25 0.50 1.00 0.25 1.00

-60 to 360/370 -60 to 360/370 -60 to 360/370 -60 to 360/370 -60 to 360/370 -60 to 360/370

7HG-G011-02 7HG-G011-11 7HG-G011-17 7HG-G011-22 7HM-G011-11 7HM-G011-22

0.25 1.00 1.00

-60 to 360/370 -60 to 360/370 -60 to 360/370

7KG-G011-11 7KG-G011-22 7KM-G011-22

Price

ZB-XLB

Price

ID(mm) 10-Meter 0.18 15-Meter 0.25 20-Meter 0.18 30-Meter 0.25 0.25 0.32 0.32 0.53 60-Meter 0.25

Price

df(µm)

Temp. Limits °C

Part No.

0.18

30 to 340/360

7CD-G019-08

0.25

30 to 340/360

7EG-G019-11

0.18

30 to 340/360

7FD-G019-08

0.25 0.50 0.25 0.50 1.50

30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 320/340

7HG-G019-11 7HG-G019-17 7HM-G019-11 7HM-G019-17 7HK-G019-28

0.25

30 to 340/360

7KG-G019-11

Price

If Zebron columns do not provide you with equivalent separations as compared to any other GC column of the same phase, and comparable dimensions, send in your comparative data within 45 days and keep the Zebron column for FREE.

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GC Columns

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p. 30

General GC Accessories

General GC Accessories Septa

Silicone Rubber Inlet Septa

• Advanced silicone formulations reduce coring and increase septum lifetime • Enhanced durability and re-sealing capabilities PhenoGrey™-250 This general purpose silicone rubber septum is designed for use up to 250 °C for extended periods of time. With a durometer reading of 40-45, septum puncture is easy (fewer bent needles) for up to 100 injections. PhenoBlue™-300 This low-bleed septum is designed from a recent technology advance in the silicone field. The high durometer rating of 50-60 guarantees over 100 injections at 300 °C, but is actually heat-stable to 350 °C to give you even greater confidence when the oven heats up! PhenoRed™-400 Temperature-rated to 400 °C, even continuous injection port temperatures of 350 °C rarely change this septum's physical properties. Typical injection life is 150 punctures. Available with or without needle GuideRight™ Hole. PhenoGreen™-400 Long life, high-temperature green septum, rated to 400 °C. GuideRight™ through-hole to guide the needle during injection.

Ferrules

Graphite Ferrules

• High-purity graphite significantly reduces ferrule bleed • Special construction minimizes "flaking"

Graphite Ferrules, Rated to 450 °C Part No. Description 0.4 mm Ferrule ID AG0-4698 Graphite Ferrule AG0-4699 Graphite Ferrule 0.5 mm Ferrule ID AG0-4701 Graphite Ferrule AG0-4702 Graphite Ferrule 0.8 mm Ferrule ID AG0-4704 Graphite Ferrule AG0-4705 Graphite Ferrule

Unit 1/16 in. to 0.4 mm 1/16 in. to 0.4 mm

10/pk 50/pk

1/16 in. to 0.5 mm 1/16 in. to 0.5 mm

10/pk 50/pk

1/16 in. to 0.8 mm 1/16 in. to 0.8 mm

10/pk 50/pk

Price

Short Ferrules

Septa Sizes by GC Instrument Manufacturer

Instrument Model

Septa Diameter

Agilent (HP)

5850, 5880, 5890, 6850, 6890 5700 series, 5880A

7/16 in. (11 mm) 3/8 in. (9.5 mm)

Silicone Rubber Septa Part No. Description PhenoGrey 3/8 in. (9.5 mm) Diameter AG0-4686 PhenoGrey-250 Injector Septa AG0-4687 PhenoGrey-250 Injector Septa PhenoBlue AG0-4688 PhenoBlue-300 Injector Septa AG0-4689 PhenoBlue-300 Injector Septa PhenoRed AG0-4690 PhenoRed-400 Injector Septa AG0-4691 PhenoRed-400 Injector Septa PhenoGrey 7/16 in. (11 mm) Diameter AG0-4692 PhenoGrey-250 Injector Septa AG0-4693 PhenoGrey-250 Injector Septa PhenoBlue AG0-4694 PhenoBlue-300 Injector Septa AG0-4695 PhenoBlue-300 Injector Septa PhenoRed AG0-4696 PhenoRed-400 Injector Septa AG0-4697 PhenoRed-400 Injector Septa

Unit

Price

50/pk 100/pk 50/pk 100/pk 50/pk 100/pk 50/pk 100/pk 50/pk 100/pk 50/pk 100/pk

Vespel/Graphite (85/15 %) Short Ferrules (not for GC/MS), Rated to 350 °C Part No. Description Similar to Mfr. No.* Unit 5181-3323 10/pk AG0-7318 Vespel/Graphite (85/15 %) Short Ferrules, 1/16 in. to 0.4 mm (for 0.10 to 0.25 mm ID columns) 5062-3514 10/pk AG0-7319 Vespel/Graphite (85/15 %) Short Ferrules, 1/16 in. to 0.5 mm (for 0.32 ID columns) 5062-3512 10/pk AG0-7320 Vespel/Graphite (85/15 %) Short Ferrules, 1/16 in. to 0.8 mm (for 0.45 to 0.53 mm ID columns) Graphite (100 %) Short Ferrules (not for GC/MS), Rated to 450 °C Part No. Description Similar to Mfr. No.* Unit AG0-7513 Graphite (100 %) 072635 10/pk Short Ferrules, 1/16 in. to 0.5 mm Vespel/Graphite (85/15 %) Short Ferrules GC/MS Compatible, Rated to 350 °C Part No. Description Similar to Mfr. No.* Unit 5181-3322 AG0-7321 Vespel/Graphite (85/15 %) GC/MS 10/pk Short Ferrules, 1/16 in. to 0.4 mm (for 0.10 to 0.25 mm ID columns) 5062-3513 10/pk AG0-7322 Vespel/Graphite (85/15 %) GC/MS Short Ferrules, 1/16 in. to 0.5 mm (for 0.32 ID columns) 5062-3511 10/pk AG0-7323 Vespel/Graphite (85/15 %) GC/MS Short Ferrules, 1/16 in. to 0.8 mm (for 0.45 to 0.53 mm ID columns)

Vespel/Graphite Ferrules (60/40 %), Rated to 400 °C Part No. Description 0.4 mm Ferrule ID AG0-4707 Vespel/Graphite Ferrule AG0-4708 Vespel/Graphite Ferrule 0.5 mm Ferrule ID AG0-4710 Vespel/Graphite Ferrule AG0-4711 Vespel/Graphite Ferrule 0.8 mm Ferrule ID AG0-4713 Vespel/Graphite Ferrule AG0-4714 Vespel/Graphite Ferrule

Unit 1/16 in. to 0.4 mm 1/16 in. to 0.4 mm

10/pk 50/pk

1/16 in. to 0.5 mm 1/16 in. to 0.5 mm

10/pk 50/pk

1/16 in. to 0.8 mm 1/16 in. to 0.8 mm

10/pk 50/pk

*Similar to but not always an exact equivalent to the original manufacturer's product.

www.phenomenex .com

Price

Price

Vespel / Graphite Ferrules

All Vespel containing ferrules should be pre-shrunk in an oven at 250 ˚C for at least 4 hours prior to use.

Web:

Price

I Email:

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Price

General GC Accessories

Ordering

General GC Accessories

Cool-Lock™ for Agilent HP GC System Part No. AG0-8319 AG0-8320 AG0-8349

Description Cool-Lock GC Capillary Nut For Use With ShortStyle Ferrules Cool-Lock GC Capillary Nut For Use With LongStyle Ferrules Cool-Lock Nut Installation Gauge

Unit ea

Price

ea

Gold Inlet Base Seals

ea

Part No. Description Similar to Mfr. No.* Unit Standard, single groove for splitless applications, 0.8 mm dia. inlet hole AG0-7518 2/pk Gold Inlet Base Seal, splitless (single 18740-20885 groove), 0.8 mm AG0-7519 10/pk Gold Inlet Base Seal, splitless (single 18740-20885 groove), 0.8 mm High Split Flow, cross groove for split applications, 0.8 mm dia. inlet hole AG0-7520 2/pk Gold Inlet Base Seal, split (double 5182-9652 groove/cross), 0.8 mm AG0-7521 10/pk Gold Inlet Base Seal, split (double 5182-9652 groove/cross), 0.8 mm Replacement Inlet Seal Washers AG0-7522 – 12/pk Inlet Seal Washers, for Agilent GC injection port

Cool-Lock™ for Shimadzu GC System Part No. AG0-8419 AG0-8420

Description Cool-Lock GC Capillary Nut For Use With ShortStyle Ferrules Cool-Lock Nut Installation Gauge

Unit ea

Price

ea

Price

Sealing O-Rings

Sealing Rings for Agilent Inlet Liners Part No. Description AG0-7326 Sealing ring, Viton, rated to < 300 °C AG0-7327 Sealing ring, graphite, rated to > 250 °C (450 °C max.)

Similar to Mfr. No.* 5180-4182

Unit 10/pk

5180-4168

10/pk

Price

Injection Port Inlet Liners Agilent Technologies (HP)

Liners Description

Column Installs This End

Splitless Straight Liner

Split / Splitless with Single Taper

Splitless, Double Taper/Gooseneck Liner

GC Model No. 5880/5890/6890

Dimensions ID x L x OD (mm) 4 x 78.5 x 6.45

Material* (deactivated) B (n)

Quartz Wool (Y / N) N

5880/5890/6890

4 x 78.5 x 6.45

B (y)

N

5880/5890/6890

4 x 78.5 x 6.45

B (y)

N

Split / Splitless with wool

5880/5890/6890 4 x 78.5 x 6.3

B (y)

Y

Split / Splitless, Single Taper with wool

5880/5980/6890 4 x 78.5 x 6.3

B (y)

Y

Notes ea 5/pk 25/pk ea 5/pk 25/pk ea 5/pk 25/pk ea

Similar to Mfr. No.** 210-3003 – 210-3003-5 20773 20774 5181-3316 – 5183-4695 20799 5183-4696 20800 5181-3315 – 5183-4705 20785 5183-4706 20786 210-4004 –

Part No. – AG0-4651 AG0-4652 – AG0-7516 AG0-7640 – AG0-8173 – AG0-8174

ea 5/pk 25/pk

5183-4711 210-4022 5183-4712 5183-4713

AG0-8175

– 22406 22407

Unit

Price

5/pk 25/pk 5/pk 25/pk 5/pk 5/pk 5/pk

* B= Borosilicate; Deactivated = Yes (y) or No (n). ** Similar to but not always an exact equivalent to the original manufacturer’s product.

Contact Phenomenex or your local Phenomenex distributor for additional GC accessories. Other manufacturer GC accessories are available.

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HPLC Columns

HPLC Luna® High Speed Technology (HST) Columns 2.5 μm High Speed Technology (HST) Columns (mm) Phase 30 x 2.0 50 x 2.0 Luna 2.5 µm C18(2)-HST

00A-4446-B0

00B-4446-B0

3 μm Microbore and Minibore Columns (mm) Phases 30 x 1.0 50 x 1.0 C8(2) C18(2) HILIC PFP(2)

00A-4248-A0 00A-4251-A0 — —

00B-4248-A0 00B-4251-A0 — —

100 x 2.0

50 x 3.0

100 x 3.0

00D-4446-B0

00B-4446-Y0

00D-4446-Y0

150 x 1.0

30 x 2.0

50 x 2.0

100 x 2.0

00F-4248-A0 00F-4251-A0 — —

00A-4248-B0 00A-4251-B0 — 00A-4447-B0

00B-4248-B0 00B-4251-B0 00B-4449-B0 00B-4447-B0

00D-4248-B0 00D-4251-B0 00D-4449-B0 00D-4447-B0

SecurityGuard™ Cartridges (mm) 150 x 2.0 4 x 2.0 /10pk 00F-4248-B0 AJ0-4289 00F-4251-B0 AJ0-4286 00F-4449-B0 AJ0-8328 00F-4447-B0 AJ0-8326 for ID: 2.0-3.0 mm

3 μm Analytical Columns (mm) Phases 50 x 3.0 C8(2) C18(2) HILIC PFP(2)

00B-4248-Y0 00B-4251-Y0 00B-4449-Y0 00B-4447-Y0

150 x 3.0

30 x 4.6

50 x 4.6

100 x 4.6

150 x 4.6

00F-4248-Y0 00F-4251-Y0 00F-4449-Y0 00F-4447-Y0

00A-4248-E0 00A-4251-E0 — —

00B-4248-E0 00B-4251-E0 — 00B-4447-E0

00D-4248-E0 00D-4251-E0 00D-4449-E0 00D-4447-E0

00F-4248-E0 00F-4251-E0 00F-4449-E0 00F-4447-E0

SecurityGuard™ Cartridges (mm) 4 x 2.0 4 x 3.0 /10 pk /10 pk AJ0-4289 AJ0-4290 AJ0-4286 AJ0-4287 AJ0-8328 AJ0-8329 AJ0-8326 AJ0-8327 for ID: 2.0-3.0 mm

5 μm Microbore and Minibore Columns (mm) Phases 30 x 2.0 50 x 2.0 C8 (2) C18 (2) PFP(2)

00A-4249-B0 00A-4252-B0 00A-4448-B0

3.2-8.0 mm

SecurityGuard Cartridges (mm) 150 x 2.0 4 x 2.0 /10 pk 00F-4249-B0 AJ0-4289 00F-4252-B0 AJ0-4286 00F-4448-B0 AJ0-8326 ™

00B-4249-B0 00B-4252-B0 00B-4448-B0

for ID: 2.0-3.0 mm

5 μm Analytical Columns (mm) Phases 50 x 3.0 C8(2) C18(2) PFP(2)

00B-4249-Y0 00B-4252-Y0 00B-4448-Y0

150 x 3.0

30 x 4.6

50 x 4.6

75 x 4.6

00F-4249-Y0 00F-4252-Y0 00F-4448-Y0

00A-4249-E0 00A-4252-E0 00A-4448-E0

00B-4249-E0 00B-4252-E0 00B-4448-E0

00C-4249-E0 00C-4252-E0 —

SecurityGuard™ Cartridges (mm) 4 x 2.0 4 x 3.0 /10 pk /10 pk AJ0-4289 AJ0-4290 AJ0-4286 AJ0-4287 AJ0-8326 AJ0-8327 for ID: 2.0-3.0 mm

3.2-8.0 mm

5 μm Analytical Columns (mm) continued SecurityGuard Cartridges (mm) Phases 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0 /10 pk C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 AJ0-4290 C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 AJ0-4287 HILIC 00D-4450-E0 00F-4450-E0 00G-4450-E0 AJ0-8329 PFP(2) 00D-4448-E0 00F-4448-E0 00G-4448-E0 AJ0-8327 ™

for ID: 3.2-8.0 mm

Synergi HPLC Columns  ™

4 µm Microbore and Minibore Columns (mm) Phases 50 x 1.0 150 x 1.0 Max-RP Hydro-RP Fusion-RP

00B-4337-A0 00B-4375-A0 00B-4424-A0

00F-4337-A0 00F-4375-A0 00F-4424-A0

250 x 1.0

30 x 2.0

50 x 2.0

100 x 2.0

– 00G-4375-A0 –

00A-4337-B0 00A-4375-B0 00A-4424-B0

00B-4337-B0 00B-4375-B0 00B-4424-B0

00D-4337-B0 00D-4375-B0 00D-4424-B0

SecurityGuard™ Cartridges (mm) 250 x 2.0 4 x 2.0 /10pk 00F-4337-B0 00G-4337-B0 AJ0-6073 00F-4375-B0 00G-4375-B0 AJ0-7510 00F-4424-B0 00G-4424-B0 AJ0-7556 150 x 2.0

for ID: 2.0-3.0 mm

4 µm Narrow Bore Columns (mm) Phases 50 x 3.0 Max-RP Hydro-RP Fusion-RP

00B-4337-Y0 00B-4375-Y0 00B-4424-Y0

4 µm Analytical Columns (mm) Phases 30 x 4.6 Max-RP Hydro-RP Fusion-RP

00A-4337-E0 00A-4375-E0 00A-4424-E0

SecurityGuard Cartridges (mm) 250 x 3.0 4 x 2.0 /10pk 00G-4337-Y0 AJ0-6073 00G-4375-Y0 AJ0-7510 00G-4424-Y0 AJ0-7556 for ID: 2.0-3.0 mm ™

75 x 3.0

100 x 3.0

150 x 3.0

00C-4337-Y0 00C-4375-Y0 00C-4424-Y0

00D-4337-Y0 00D-4375-Y0 00D-4424-Y0

00F-4337-Y0 00F-4375-Y0 00F-4424-Y0

50 x 4.6

100 x 4.6

150 x 4.6

00B-4337-E0 00B-4375-E0 00B-4424-E0

00D-4337-E0 00D-4375-E0 00D-4424-E0

00F-4337-E0 00F-4375-E0 00F-4424-E0

SecurityGuard™ Cartridges (mm) 250 x 4.6 4 x 3.0 /10pk 00G-4337-E0 AJ0-6074 00G-4375-E0 AJ0-7511 00G-4424-E0 AJ0-7557 for ID: 3.2-8.0 mm SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

Web:

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I Email:

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HPLC Columns

Ordering

HPLC Gemini® 3 μm Microbore, Minibore and Narrow Bore Columns (mm) Phases 50 x 1.0 30 x 2.0 50 x 2.0 C18

SecurityGuard™ Cartridges (mm) 150 x 3.0 4 x 2.0* /10pk 00F-4439-Y0 AJ0-7596 for ID: 2.0-3.0 mm

100 x 3.0

00A-4439-E0

00A-4435-B0

00A-4435-E0

SecurityGuard™ Cartridges (mm) 250 x 4.6 4 x 3.0* /10pk 00G-4439-E0 AJ0-7597 for ID: 3.2-8.0 mm

100 x 4.6

150 x 4.6

00B-4439-E0

00D-4439-E0

00F-4439-E0

150 x 2.0

250 x 2.0

50 x 3.0

100 x 3.0

150 x 3.0

00B-4435-B0

00F-4435-B0

00G-4435-B0

00B-4435-Y0

00D-4435-Y0

00F-4435-Y0

50 x 4.6

100 x 4.6

150 x 4.6

00B-4435-E0

00D-4435-E0

00F-4435-E0

5 μm Analytical Columns (mm) Phases 30 x 4.6 C18

50 x 3.0

50 x 4.6

5 μm Minibore and Narrow Bore Columns (mm) Phases 30 x 2.0 50 x 2.0 C18

150 x 2.0

00B-4439-A0 00A-4439-B0 00B-4439-B0 00D-4439-B0 00F-4439-B0 00B-4439-Y0 00D-4439-Y0

3 μm Analytical Columns (mm) Phases 30 x 4.6 C18

100 x 2.0

SecurityGuard™ Cartridges (mm) 250 x 3.0 4 x 2.0* /10pk 00G-4435-Y0 AJ0-7596 for ID: 2.0-3.0 mm

SecurityGuard™ Cartridges (mm) 250 x 4.6 4 x 3.0* /10pk 00G-4435-E0 AJ0-7597 for ID: 3.2-8.0 mm

* SecurityGuard Analytical Cartridges Require Holder, Part No.: KJ0-4282

MercuryMS® Cartridges and Columns Cartridges (mm) Qty Pricing 2.5 μm Synergi Synergi Synergi 3 μm Luna Luna Gemini 5 μm Luna Luna Gemini

10 x 2.0 1 @ /ea 3 @ /ea 5 @ /ea 10 @ /ea



10 x 4.0 1 @ /ea 3 @ /ea 5 @ /ea 10 @ /ea



20 x 2.0 1 @ /ea 3 @ /ea 5 @ /ea 10 @ /ea



20 x 4.0 1 @ /ea 3 @ /ea 5 @ /ea 10 @ /ea

Columns (mm) 20 x 2.0

20 x 4.0

— — —

— — —

Phase Hydro-RP Max-RP Fusion-RP

00N-4387-B0-CE 00N-4372-B0-CE 00N-4423-B0-CE

00N-4387-D0-CE 00N-4372-D0-CE 00N-4423-D0-CE

00M-4387-B0-CE 00M-4372-B0-CE 00M-4423-B0-CE

00M-4387-D0-CE 00M-4372-D0-CE 00M-4423-D0-CE

00M-4387-B0 00M-4372-B0 00M-4423-B0

00M-4387-D0 00M-4372-D0 00M-4423-D0

C18(2) C8(2) C18

00N-4251-B0-CE 00N-4248-B0-CE Inquire

00N-4251-D0-CE 00N-4248-D0-CE Inquire

00M-4251-B0-CE 00M-4248-B0-CE 00M-4439-B0-CE

00M-4251-D0-CE 00M-4248-D0-CE 00M-4439-D0-CE

00M-4251-B0 00M-4248-B0 00M-4439-B0

00M-4251-D0 00M-4248-D0 00M-4439-D0

C18(2) C8(2) C18

00N-4252-B0-CE 00N-4249-B0-CE ­—

00N-4252-D0-CE 00N-4249-D0-CE —

00M-4252-B0-CE 00M-4249-B0-CE 00M-4435-B0-CE

00M-4252-D0-CE 00M-4249-D0-CE 00M-4435-D0-CE

— — —

— — —

MercuryMS™ Cartridge Holders Standard Cartridge Holders Part No. Description CH0-5846 10 mm standard holder CH0-5845 20 mm standard holder Direct-Connect Cartridge Holders Part No. Description CH0-7187 10 mm direct-connect holder CH0-7188 20 mm direct-connect holder

Price

Price

Trademarks

Gemini, Luna, and Strata are registered trademarks of Phenomenex, Inc. Cool-Lock, MercuryMS, PhenoBlue, PhenoGreen, PhenoGrey, PhenoRed, Strata-X, Synergi, and Zebron are trademarks of Phenomenex, Inc. Strata-X is patented by Phenomenex, Inc. Bond Elute Certify is a registered trademark of Varian, Inc. Clean Screen and XtrackT are registered trademarks of United Chemical Technologies, Inc. Narc-2 is a trademark of Mallinckrodt Baker, Inc. ISOLUTE is a registered trademark of Biotage. Oasis is a registered trademark of Waters Corp.

If Luna, Gemini, or Synergi analytical columns do not perform as well or better than your current HPLC column of similar phase, mass and size, send in your comparative data within 45 days and keep the Gemini, Luna or Synergi analytical column for FREE!

Disclaimer

Phenomenex is not affiliated with Agilent, Varian, Inc., United Chemical Technologies, Inc., Mallinckrodt Baker, Inc., or Biotage. Subject to Phenomenex Standard Terms and

Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

© 2009 Phenomenex, Inc. All rights reserved.

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Australia

t: 02-9428-6444 f: 02-9428-6445

[email protected]

Austria

t: 01-319-1301 f: 01-319-1300

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Belgium

t: +31 (0)30-2418700 f: +31 (0)30-2383749

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Canada

t: (800) 543-3681 f: (310) 328-7768

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t: 4824 8048 f: 4810 6265

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France

t: 01 30 09 21 10 f: 01 30 09 21 11

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Germany

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t: 01 247 5405 f: +44 1625-501796

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United Kingdom

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All other countries: Corporate Office USA

t: (310) 212-0555 f:

www.phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]

BR63970808_I

(310) 328-7768 [email protected]