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IBSC Facilities Manual
The purpose of this document is to outline the operational aspects of the IBSC Shared FACS and TC cores. This information includes inventory summary, space, and equipment maintenance and repair, recharge process and Facilities contacts. Additional training material is attached as appendices. The IBSC shared FACS and TC laboratory space is physically located in two building on Science Hill here at UCSC. Biomed Spaces and equipment: 415A Beckman Coulter Avanti Ultracentrifuge 415B Glassware washing facility 415D Teaching dissecting scope, Inverted fluorescent scope 415E Agilent Bioanalyzer, ABI Real Time PCR 435A Biorad Imaging doc 435B Murine Electrophysiology Rig setup 435C Cold Room 445A CBA Liq. N2 cryo storage, CO2 manifold, Shaker 445B Warm Room, shaker 445C BD FACS Aria cell sorter 445D Diagneode ChIP, Diagneode Bioruptor II 461 BD FACS LSRII analysis cytometer, Zeiss liveimaging microscope 463 Viral Tissue Culture, Ultracentrifuge, hypoxia incubator 464A Shared Stem Cell TC, IVF-‐workstation, hypoxia incubator 365 Leica Cryostat, Leica CryoJane, Microtome
072520164 BN Sinsheimer Spaces: 106 SSCF Human esc Tissue Culture 109 SSCF main lab core 109A SSCF general use, CO2 manifold, BD FACS Calibur cytometer 109B Mouse esc TC and Teaching lab Vivarium Spaces: 15D CIRM procedure room Vivarium 15E CIRM Animal Holding area Vivarium 55C Mixed use equipment
Note: Janitorial support is not used for any of the Tissue Culture Facilities. IBSC Manager mops floors in TC labs 3 times weekly. Floors are stripped and waxed every 3 years, but you must contact Custodial Services to make arrangements. Carpet cleaning is never provided so you need to put in a Facilities Work Order at: http://physicalplant.ucsc.edu/request-‐services/request-‐workorder.html Shared Conference room 460, and Post-‐doc space is monitored by IBSC Manager.
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Shared Equipment Repair Contacts (for equipment not listed or covered by vendor service agreements, please contact building repair technician, Cheri Everlove at: Instrument Serial # Vendor ABI Real time PCR
278881015
ThermoFisher
PO#
Exp. Date
PM Due
Phone
NA
June 2017
June
800-‐762-‐4001
FACS Aria sorter P46900093
BD Biosystems P0497362
Oct. 11, 2018
Jan/June
877-‐232-‐8995
FACS LSRII analyzer Cryostat 3050
H55100016
Cytek
P0570164
March 1, 2020
Jan/June
510-‐657-‐0102
127000259
Leica
NA
May 31, 2017
August
May 2017
May
800 248 0123 510-‐845-‐5591
Biosafety Multiple TSS Yearly PO Cabinets SSCF/IBSC Agilent Bioanalyzer 2100 (DEW0186873): 1-‐800-‐227-‐9776 Diagneode Bioruptor II (BRU20172): 1-‐862 209-‐4680 Diagenode ChIP (G8GCF006): 1-‐862 209-‐4680 Origio IVF hood and scope(12JWSM1001): 1-‐800-‐444-‐8456 Millipore Water Purification Systems: 1-‐800-‐645-‐5476 Kitchen water filter system: Jeff Duncan 831-‐459-‐5710
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General Facilities Contacts: IBSC Administrative Director: Ann Pace IBSC Oversight Committee: Lindsay Hinck Camilla Forsberg Sofie Salama Nadine Gassner Ben Abrahms UCSC Environmental Health and Safety Contacts: 1. Biosafety: Karianne Terry, 459-‐3542,
[email protected] 2. General Lab Safety: Lisa Wisser, 459-‐5772,
[email protected] 3. Radiation: Tristan White, 459-‐3911,
[email protected] 3. PSB Safety Coordinator: Donna Riggs,
[email protected] 4. Online Training Link: http://learningcenter.ucsc.edu/ Plumbing, electrical, HVAC: Campus work order desk: 9-‐4444 Equipment moves, Alarm setup, general lab needs: SOE Facilities 9-‐5710 PBSci Card Key Access (Biomed): Pat Kent 9-‐1992 SOE Brass Key Access (SSCF): Al McGuire 9-‐4878 SOE Space: Bob Vitale 9-‐3794
072520164 BN PSB Facilities Manager: Hal Kuhns 9-‐2810 Compressed Gases: AirGas delivery and pickup: Salinas Store 1-‐831-‐422-‐4743 Tissue Culture Training Procedures New Users 1. See Facilities Manager to discuss the project and needs. 2. Submit the IBSC Safety Form: https://stemcell.soe.ucsc.edu/facilities/safety_questionnaire 3. Submit a new or amended BUA for any BSL2 related projects. Contact Biosafety officer for more information. 4. Once approved, schedule a time to meet with Facilities Manager to go through an individualized TC orientation/training. FACS Training Procedures New Users 1. Contact IBSC Facs Manager to schedule a time to meet and discuss your project goals. 2. Submit the IBSC Safety Form: https://stemcell.soe.ucsc.edu/facilities/safety_questionnaire 3. Submit a new or amended BUA for any BSL2 related projects. Contact Biosafety officer for more information. 4. Once approved, schedule a time to meet with Facilities Manager to go through an individualized FACS orientation/training.
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Laboratory Maintenance and Support
The Facilities Manager and one part-‐time student Lab Assistant maintain the IBSC Shared Tissue Culture and FACS Facilities. The operational support includes general janitorial maintenance of shared spaces, biological waste disposal, glassware washing and autoclaving, supply stocking (see consumables below) and inventory (including reagent prep for FACS instrument cleaning), incubator calibration and cleaning, general equipment maintenance, ensure safety compliance, and equipment inventory tracking. Tissue Culture maintenance is done daily. Users are expected to help during extended building shutdowns, and when Facilities personnel are unavailable. The shared Tissue Cultures and Flow Cytometry spaces are Biosafety Level 2. All applicable regulations apply. Yearly Biosafety cabinet certification and EH&S Laboratory Inspections are done for compliance. General Supplies provided by the IBSC Shared Facility Tissue Culture Compressed Gases: (purchased through Air Gas, Yearly Blanket PO) 1. Liquid Nitrogen NI 265LT22 200 265 liter, 22 PSI 2. CO2 CD BD200, 200 size Bone Dry 3. Nitrogen NI UHP200, 200 size Ultra High Purity Consumables: 1. Ethanol 70% 2. Bleach 100% and 10%
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FACS
3. Paper towels 4. Vacuum Line Filters 5. Gloves 6. Kim wipes 7. Biosafety collection products ( Sharps, autoclave bags) 8. General janitorial supplies (waste bags, cleaning solutions) 9. Anti-‐microbial soap and hand sanitizer 10. Assorted waters: Distilled, DI, Ultrapure 11. Assorted autoclaved glassware 12. Safety glasses/goggles 13. Autoclave supplies (tape, wraps) 14. Sharps containers 15. Glassware wash solutions: detergent and acid 16. Microscope bulbs
1. BD Falcon 12x 75 tubes (polystyrene and polypropylene) 2. Nitex Filtering nylon 3. Pipetman and tips (P-‐200 and P-‐1000) 4. Transfer pipettes 5. 15 and 50 ml conical tubes 6. Kimwipes 7. Paper towels 8. Ethanol 9. Bleach 100% and 10% 10. Coulter Clenz 11. Contrad 12. Sample Line filters 13. Filtered ultrapure water
072520164 BN 14. Autoclaved Sheath (PBS) 15. Sorting nozzles (70, 85, 100, 130um) 16. Cotton tipped applicators 17. Mini nozzle scope and batteries 18. Tank connector and lid o-‐rings 19. Gloves 20. Safety goggles/masks 21. Sort collection and acquisition devices (eppendorf, 12x75, 215 ml) 22. Sheath and Waste sensors 23. Aerosol containment filters 24. First Aid kits 25. Tube racks 26. Calibration beads 27. Data transfer flashes 28. FloJo Software analysis dongles 29. Mod-‐Fit DNA analysis software 30. Printer Cartridges 31. Printer paper 32. Printer misc. supplies
FACS Recharge
1. Cytometer generated user login csv. files sorted by 7th month. 2. Sorted files are tested using SOE online testing site: https://recharge-‐test.soe.ucsc.edu/user/login?destination=recharge 3. Once they are confirmed for correctness, the files are uploaded to: https://recharge.soe.ucsc.edu/user/login?destination=recharge
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4. PI’s receive invoices, including user, dates details, and assign a FOAPAL. 5. After PI approval, invoices automatically get distributed to Research Accountants for final approval processing.
Sinsheimer SSCF TC Recharge 1. This is a manual process but does upload to the same Recharge Online System, although uploads as Bulk file. 2. Use this excel format to upload. Save as Windows We have 3 codes.
TCLN01 TCWEEK01 NUT01
Liq. N2 ($25/box/month) TC use per week ($45.00) New user ($200)
Cruzid Approver
short recharge code
foapal org1
foapal fund1
foapal activity code1
bnazario
TCWEEK01
8 TC use SSCF
Apr-‐10-‐15
bnazario
TCWEEK01
2 TC use SSCF
Apr-‐10-‐15
bnazario
TCWEEK01
1 TC use SSCF
Apr-‐10-‐15
TCWEEK01
5 TC use SSCF
Apr-‐10-‐15
ssalama
ssalama
TCWEEK01
2 TC use SSCF
Apr-‐10-‐15
ssalama
TCWEEK01
1 TC use SSCF
Apr-‐10-‐15
number units
description
servicedate
percent1
foapal org2
foapal fund2
f a c
