home of tissue culture
®
Tissue Culture and Laboratory Technology Sales Brochure 2014/2015
www.tpp.ch
Contents
Welcome
2–3
Tissue Culture Flask
4–5
Tissue Culture Flask with Peel-Off Foil Tissue Culture Flask with Re-Closable Lid
6–7
Tissue Culture Flat Tube 10 Tissue Culture Tube 20
8–9
Cell Scraper Cell Spatula
10–11
TubeSpin® Bioreactor
12–13
Adapter Plate for Shaker TubeSpin® Bioreactor with Septum
14–15
PCV Packed Cell Volume Tube “easy read” Measuring Device
16–17
Serological Pipette Pipettor Turbo-Fix
18–19
Tissue Culture Test Plate
20–21
Tissue Culture Dish
22–23
Vacuum Filtration “rapid“-Filtermax Syringe Filter
24–25
Centrifuge Tube
26–27
Cryo Tube
28–29
Rack for Tubes Cryo Box
30–31
3-B Product
32–33
General Information Quality Standards Transportation and Storage
34–37
Quick Review
38–40
TPP Techno Plastic Products AG
2
1
3
4
5
TPP products are developed and manufactured conforming ISO 9001 norms. They are available worldwide through the TPP dealer net.
7
6
8
9
10 1
Entrance
2
Storage house of finished goods
3
Silos for plastic granulate
4-5
Design engineering
6-10
Production
11-13
Quality control
14-15 Automated guided vehicle system
2–3
Dear TPP customer For more than 50 years – and with me in the 2nd generation – TPP has been a successful manufacturer for disposable plastic products for tissue culture the highest quality. I am aware, that all our esteemed customers judge the TPP product not only by its product quality but also by the customer service accompanying it. In order to fulfill all these demands and requests to the full extent, us – together with a worldwide net of local TPP dealers – commit ourselves with pleasure and enthusiasm. 100 % customer satisfaction regarding quality and customer service is my personal target. So my staff, the TPP dealers and I are engaged with utmost motivation and top state of the art machinery, engine plants, infrastructure and personal assignment to be able to serve you with the worldwide best quality and service on each level possible.
Worldwide a growing number of content TPP product users.
The TPP team and I are happy to work for and with you. Rolf Tanner
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14
15
Tissue Culture Flask 25– 300 cm2
Features of the Tissue Culture Flask: • Surface activated, uniform flat growth surface • Geometry of the neck reduces risk of media slop over • Crystal clear transparency • Stable, slip-free stacking of multiple flasks (picture 2, following page) • Highly visible bilateral marking areas • White volume graduations for optic control of the filling volume But also: • Due to geometry of the neck no dead corners = 100 % cell retrieval • Two cap styles available: Filter screw cap or «VENT» screw cap
The hydrophobic PTFE membrane with pore size 0.22 µm of the Filter screw cap offers protection to contamination and optimal sterile gas exchange at the same time. The TPP click • A click and the «VENT» screw cap is in the aeration-position. s Visual control: the slightly raised rectangle is at 12 o’clock. • Close the flask gas-tight by turning the «VENT» screw cap clockwise 90 (picture 2). s Visual control: the slightly raised rectangle is at 3 o’clock.
The opto-mechanical surface activation from TPP enhances even and consistent cell adhesion and cell growth.
1
2
1 Aeration position of «VENT» screw cap, 12 o’clock. 2 Closed gas-tight, 3 o’clock.
4-5
1
2
3
1 100 % access of pipettes, tissue scrapers and the tissue spatula from TPP. 2 Stable and slip-free stacking of multiple flasks. 3 White marking area with lines to support your identification.
Type
Type
4
Product-No.
Growth surface cm2
Version
Volume ml
Dimensions lxwxh mm
Material
Qty / Bag
Qty / Case
90025
25
«VENT»
recom.= 3–5, max = 15
92 x 51 x 29
PS
10
360
90026
25
Filter
recom.= 3–5, max = 15
92 x 51 x 29
PS
10
360
90075
75
«VENT»
recom.= 8–15, max = 65
155 x 87 x 40
PS
5
100
90076
75
Filter
recom.= 8–15, max = 65
155 x 87 x 40
PS
5
100
90150
150
«VENT»
recom.=15–30, max = 165
210 x 122 x 50
PS
3
36
90151
150
Filter
recom.=15–30, max = 165
210 x 122 x 50
PS
3
36
90300
300
«VENT»
recom.=30–40, max = 410
275 x 170 x 50
PS
3
18
90301
300
Filter
recom.=30–40, max = 410
275 x 170 x 50
PS
3
18
Dimensions Øxh mm
Material
Qty / Bag
Qty / Case
Version
Product-No.
90825
«VENT» for T-25
24 x 16
PE
10
40
90256
Filter for T-25
24 x 16
PE
10
40
90875
«VENT» for T-75
33 x 21
PE
10
40
90876
Filter for T-75
33 x 21
PE
10
40
90850
«VENT» for T-150/300
39 x 24
PE
10
40
90856
Filter for T-150/300
39 x 24
PE
10
40
5
4 “VENT“ and Filter screw caps. 5 Filter screw cap with a hydrophobic PTFE membrane, pore size 0.22 µm welded in.
Tissue Culture Flask with Re-Closable Lid or Peel-Off Foil
Features of the unique Tissue Culture Flask with multi-use Re-Closable Lid: • A multi-use re-closable liquid-tight lid • Complete access from above to flask chamber (picture 3) • Stable, slip-free stacking of multiple flasks (picture 1) also in combination with the standard TPP tissue culture flasks • Optionally available with barrier which creates a growth area of 115 cm2
Features of the Tissue Culture Flask with Peel-Off Foil: • Foil is peeled off before access to the cells • Multilayer foil material is bonded on the polystyrene carrier • Sterility is maintained until cells or tissue are harvested • Optionally available with barrier which creates a growth area of 115 cm2 • Re-closure of this flask is not possible
Both flasks are available with Filter screw cap only. If a «VENT» screw cap is used, the build up of pressure could press open the flask.
1 Secure stacking of multiple flasks. 2. A unique click-in system allow the lid to be fixed into any desired position. 3. Complete access to cell chamber from above.
1
2
3
6-7
Opening and re-closing mechanism.
1
1 The TPP tissue culture flasks with convenient re-closable lid.
2
2 The foil can be gripped and peeled off at the given flap with ease.
Type Product-No.
3
Growth surface cm2
Version
Lid Opening mm
Volume ml
Dimensions lxwxh mm
Material
Qty / Bag
Qty/ Case
90551
150
peel-off foil
100 x 110
recom.=15–30, max = 100
210 x 122 x 50
PS
3
18
90552
150
lid
105 x 105
recom.=15–30, max = 100
210 x 122 x 50
PS
3
18
90651
115
peel-off foil, with barrier 100 x 110
recom.=15–30, max = 100
210 x 122 x 50
PS
3
18
90652
115
105 x 105
recom.=15–30, max = 100
210 x 122 x 50
PS
3
18
lid, with barrier
3 Both TPP flasks – with re-closable lid or with peel-off foil – are leak tight up to 100 ml filling volume.
Tissue Culture Flat Tube 10 and Tissue Culture Tube 20
The tissue culture tubes are an extension to the tissue culture flask product range. Features of the Tissue Culture Flat Tube 10: • 3-in-1 product: cultivate, examine and spin cells in the same vessel! • Cultivation: 10 cm2 surface activated growth area • Large opening for complete access of pipettes and scrappers (picture 1) • Examination: great optical clarity for visual control with inverse microscopes (picture 2) • Sloped and flattened upper side for reduced refraction • Filter screw cap with hydrophobic PTFE membrane pore size 0.22 µm • Centrifugation: fits any 50 ml standard tube adapter, can be spun up to 1’200 xg • Conical form eases removal of pellet
Features of the Tissue Culture Tube 20: • 20 cm2 surface activated growth area • Growth area for a length of 50 mm • Volume graduations up to the tip • specially formed 10-sided comb from «VENT» screw cap with “Click” avoids inadvertent rolling and enables regaining previous positions • Fits a 15 ml standard tube adapter, can be spun up to 1’200 xg • Convenient re-closable zipper bag with laser perforation (picture 5, following page)
The TPP tissue culture rack fits 8 x tissue culture flat tubes 10 in a horizontal position or 8 x tissue culture tubes 20 for storage in a CO2 incubator. 1 Unique tissue culture flat tube 10 with Filter screw cap. 2 The firm horizontal stand simplifies the readjusting of the microscope.
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2
8-9
1
2
1 Cell scraper handling in tissue culture flat tube 10. 2 Tissue growth area for a length of 50 mm.
Laser perforation: The laser perforation eases the first opening of the bag, no auxiliary opening aids are required. In addition an intact laser perforation functions as security seal. Zipper: The re-closable mechanism of the zipper enables an easy, multiple opening and closing of the bag. This makes sense with bags with large contents (picture 5).
Type
Product-No.
Packaging
Growth surface cm2
Version
Volume ml
g-Force max. xg
Dimensions Øxl mm
Material
Qty / Bag
Qty / Case
91106
Laser perforation, Zipper
20
«VENT»
5
1’200
16 x 120
PS
20
800
91243
Laser perforation
10
Filter
10
1’200
30 x 105
PS
4
216
99018 Product-No.
Version
Qty / Bag
28 / Qty Case
99018
Rack
1
28
Rack Capacity
8 x 91243 oder 8 x 91106
Dimensions lxwxh mm 200 x 88 x 72
8 x 91243 oder 8 x 91106
86 x 197 Material x 71 PP
PP
3 Tube rack for tissue culture tubes. 4 Segmental packaging allows scratch-free transportation and handling. 5 Laser perforation.
3
4
5
Cell Scraper and Cell Spatula
A slight pressure on the handle and simultaneous minimal twist of the hand suffices to pivot the scraper head to the desired direction (picture 1). Pivoting head negates need to remove scraper head to re-position and lowers contamination risk. By this you receive thorough cell retrieval from vessels of many shapes and sizes. Features of the Cell Scraper: • Scraper heads made from a flexible polyethylene material • Pivoting scraper head for easy maneuvering • Different blade width • Small raised knobs on handle for secure grip • Lint free plastic/paper blister (picture 2) • User friendly dispenser case
The cell spatula features a specially formed blade with a sharp edge. It is commonly used with the TPP tissue culture flask with re-closable lid or peel-off foil or with large tissue culture dishes. Features of the Cell Spatula: • Special formed blade with sharp edges • Small raised knobs on handle for secure grip • Lint free plastic/paper blister (picture 2) • User friendly dispenser case
1 Pivot the scraper head by slight pressure on the handle with the forefinger thus pushing the handle downwards. Slight move of the handle will twist the head to the required position. 2 Small raised knobs on the handles for a secure grip. 3 Different blade widths.
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2
3
10-11
2
1
1 Usage of cell scraper in a large TPP tissue culture dish. 2 Specially formed blade of cell spatula.
Type
Product-No.
3
Product-No.
Length mm
Version
Width of Blade mm
Material
Qty / Bag
Qty / Case
99002
240
rotating
13
PP/PE
1
150
99003
300
rotating
20
PP/PE
1
150
99004
380
rotating
25
PP/PE
1
100
99010
195
fix
14
PP
1
150
Field of Application
99002
tissue culture flask: 25/75 cm2, tissue culture flat tube 10, tissue culture dish: Ø 40/60 mm
99003
tissue culture flask: 75/150 cm2, tissue culture dish: Ø 100/150 mm
99004
tissue culture flask: 300 cm2
99010
tissue culture dish 150, many other applications
4
3 The cell scraper head is made of soft polyethylene to protect the cells. 4 Convenient user friendly TPP-dispenser.
TubeSpin® Bioreactor 15–600
TubeSpin® bioreactors are ideal for large scale screenings and optimization of suspension cell system process developments. The important parameters for the production up-scaling can be evaluated with small amounts. The cultivation is performed in shakers at a temperature of 37 °C. With a high cell density the supply of oxygen through the openings above the gas permeable, sterile PTFE filter is sufficient and optimal results can be expected. Features of TubeSpin® Bioreactor 15 • Working volume 1 – 15 ml • 2 versions: conical or round bottom • Filter screw cap with 5 different openings (A-B-C-D-E) • Fits standard 15 ml centrifuge apparatus • Spin: 15’500 xg
Features of the TubeSpin® Bioreactor 50: • Working volume 1 – 35 ml • Conical bottom • Filter screw cap with 5 different openings (A-B-C-D-E) • Fits any standard 50 ml centrifuge apparatus • Spin: 15’500 xg Features of the TubeSpin® Bioreactor 600: • Working volume up to approx. 400 ml • Conical bottom • Filter screw cap with 10 openings • Fits many 1’000 ml rotors • Spin: 3’500 xg
1 Openings can be individually sealed. 2 Racks with TubeSpin® Bioreactors. 3 TPP rack for large vessels, such as the TubeSpin® Bioreactor 600.
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2
3
12–13
1 Due to the conical bottom form, no transfer of the cells is necessary. Cells can be sediment directly in the TubeSpin® Bioreactor 600.
1
2 Openings in the filter screw cap for sterile gas exchange through the PTFE-membrane.
2
Type
Type
Product-No.
Version
g-Force max. xg
Volume ml
Dimensions Øxl mm
Material
Qty / Bag
Qty / Case
87015
15 ml, conical, filter, 5 openings
15’500
15
17.1 x 120
PP
40
800
87017
15 ml, round, filter, 5 openings
15’500
15
17.1 x 120
PP
40
800
87050
50 ml, conical, filter, 5 openings
15’500
50
30.0 x 115
PP
20
180
87600
50 ml, conical, filter, 10 openings
3’500
600
98.0 x 181
PP
1
26
Product-No.
Version
Capacity ml
Dimensions lxwxh mm
Material
Qty / Bag
Qty / Case
99013
rack for 87600
3 x 87600
366 x 146 x 70
PP
1
6
99017
rack for 87017 and 87050
18 x 15 ml tube 10 x 15 ml tube
209 x 98 x 62
PP
1
45
A
B
C
D
E
87015 Bioreactor 15
0.4 mm
0.6 mm
1.0 mm
1.25 mm
1.5 mm
87017 Bioreactor 15
0.4 mm
0.6 mm
1.0 mm
1.25 mm
1.5 mm
87050 Bioreactor 50
0.4 mm
0.6 mm
1.0 mm
1.5 mm
2.0 mm
87600 Bioreactor 600
10 openings with size 4 mm
Troubleshooting for shaken bioreactor users is offered by “Dr. Shaker“, i.e. specialists from sciences and industry.
The “Forum Shaking Technology“ is a cooperation of competent partner companies in the field of laboratory technology and biotechnology. The free publication database offers a broad selection of scientific papers on shaken cultures in micro titre platforms containing up to 1’000 l reactors. Developers and users of shaken bioreactors find on these internet pages comprehensive information. www.shakingtechnology.com
Adapter Plate for Shaker
For the usage of TubeSpin® Bioreactor 600 in shakers, TPP offers special adapter plates for shakers from Kühner AG and Infors AG.
Type
Features of adapter plates • Fits the relevant shakers • Guarantees largest possible volume per adapter plate • Secure fixation of the tubes with holding bars
Product-No.
Version
Volume ml
Dimensions lxwxh mm
Material
Qty / Bag
Qty / Case
87611
adapter plate 32 Kühner
8x4
800 x 420 x 139
Alu
1
1
87612
adapter plate 20 Kühner
5x4
500 x 420 x 139
Alu
1
1
87613
adapter plate 16 Kühner
4x4
420 x 420 x 139
Alu
1
1
87631
adapter plate 32 Infors
8x4
850 x 470 x 139
Alu
1
1
1 Adapter plate 16. 2 Adapter plate 20. 3 secure fixation with holding bars.
1
2
3
TubeSpin® Bioreactor 50 with Septum
14–15
The TubeSpin® Bioreactor 50 with Septum is intended for use during laboratory automations or robotics use. Features of the TubeSpin® Bioreactor 50 with Septum • Re-closable cross slit for multiple entries • Compatible for injection needles up to 2.1 mm diameter • Working volume
