GHRC - Global HIV Vaccine Research Cryorepository
Global HIV Vaccine Research Cryorepository - GHRC
Global HIV Vaccine Research Cryorepository – GHRC
CAVD – Collaboration for AIDS Vaccine Discovery •
> 165 investigators in 22 countries from 89 institutions – 16 Principal Investigators: 11 in US, 5 in Europe – Australia, Austria, Belgium, Brazil, Cameroon, Canada, China, Denmark, France, Germany, India, Japan, Netherlands, South Africa, Spain, Sweden, Switzerland, Thailand, Uganda, UK, US, and Zambia
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11 Vaccine Discovery Consortia (VDCs) – 5 focused on neutralizing antibodies – 6 focused on cell mediated immunity
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5 Central Service Facilities (CSFs) – 2 Vaccine immune Monitoring Centers – 1 Mouse Immunology Lab – 1 Specimen repository – 1 Statistical Center
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Alliance Management Contract
Global HIV Vaccine Research Cryorepository – GHRC
Vaccine Discovery Consortia Antibody Stamatatos
Letvin
Haynes
Weiss
Lehner
Patterson
Cellular immunity Zamb
Pantaleo
Zolla-Pazner McElrath Self
Von Briesen
Koup
Montefiori Greenberg
Central Service Facilities Global HIV Vaccine Research Cryorepository – GHRC
Ho
GHRC – Major Aims
The “CAVD Consortium on Global HIV Vaccine Research Cryorepository (GHRC)“ implies the establishment of a new and unique cryorepository. ! The establishment of a CAVD specific global HIV specimen collection ! Clinical samples: plasma, PBMC, virus isolates ! Key reagents from CAVD consortia: vectors, candidate vaccines, peptides, antibodies, rec. proteins
! New strategies of sample collection (e.g. “early infections”). ! New quality of preservation technology and sample processing. ! Future-proof technology for collecting, preparing, conserving and distributing reagents for extended networks. ! Technology upgrade of primary sites and transfer of operating procedures to and exchange of specimens with other VIMCs and VDCs. Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Objectives Coordination & Management Networking, Training and Logistics
Research on HIV Specific Cryoprocedures
GHRC Development of a HIV Cryotechnology Platform
Fully Operational HIV Cryobank Evaluation of Dedicated Cryotechnologies, Tools & Procedures Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Research on HIV Specific Cryoprocedures
Primary sites
Whole blood (~40ml)
serum / plasma
PBMC
Viral RNA
virus isolates
Primary sites and/or Cryobank
Cryobank
Cryopreservation
Neutralization assays
Full length cloning
Fulllength sequencing
Full-length cloning
T-cell assays
Partner
! Optimized sample processing protocols ! Optimized cryoprotectants ! New strategies (e.g. cells on surfaces) Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Development of a HIV Cryotechnology Platform
Rack access without white frost and fog
!HIV cryotank with access tower !HIV-specific cryosubstrates !Cryogenic memory cards !Pipetting robot
Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Development of temperature and ice protection hood system "Warm" protection space 0 % humidity
Isolated cryogenic work space with temperature controlled nitrogen atmosphere
Isolated cryogenic storage space
Basis vessel Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Prototypes of HIV cryosubstrates
250 !l 500 !l
100 !l
Substrate with coupled memory device Well-Plate with electrical contacts Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Fully Operational HIV Cryobank (P3) Co-funding by Government of Saarland and FhG
Specimen processing •Cryoprocedures •Quality control •Shipment
Technology •Operation of cryotanks •Cryogenic electronic infrastructure •Shipment
Data management •Webinterface •Administration (e.g. backup) •Connection to VISC, VIMC, VDC
Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Cryo Collections
! clinical specimen collections e.g. stem cells ! backup-collections ! biological and material research specimens
Global HIV Vaccine Research Cryorepository – GHRC
GHRC
Cryo Control Room
Cryo-Security-Area
! over 14,000 sqft floorspace for cryovessel installation ! over 75,000 gallons of cryospace (at -250 F) ! today about 5,000 gallons (at –250 F) installed Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Networking, Training and Logistics
! Selection of Primary Sites (PS) ! Transfer of cryoequipment & procedures to PS ! Training workshops ! Technology transfer to VIMCs and VDCs ! Collaboration with VISC ! Reagent distribution (NIBSC, NIH)
Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Overall Milestones Selection of Primary Sites with access to early infections completed
M12
P3 laboratory ready for operation including certification
M15
Specimen-specific cryosubstrates and manual tools developed and evaluated
M24
SOPs for optimized cryopreservation of PBMCs using validated assays for measuring immune reactive T cells completed
M24
50 to 100 full-length viral clones & sequences acquired
M36
Evaluation of operational cryobank and P3 laboratory by the GHAVE Network
M36
Global HIV Vaccine Research Cryorepository – GHRC
GHRC – Coordination & Management Centralized Facility FhG-IBMT
Coordination
Evaluation
Research CryoProcedure s
Cryo Devices
Optimized Specimen Processing
UdS DIBIT
2 VIMCs MIL
11 VDCs 11 VDCs
Data Management & Central Database
Technology Platform Substrates
Low Temp Electronics
Specimens VISC
Cryobank Network and Logistics & Special Equipment Training and Capacity Building + SOPs
ULund UdS FhGIBMT
Primary Sites Cohorts: early infections (< 6 months)
Global HIV Vaccine Research Cryorepository – GHRC
NIBSC NIH WHO
GHRC – Mobile Laboratory Unit for Sample Collection, Diagnostic and Sample Processing Co-funding by FhG
Ausziehbarer
Ggf auch noch ausziehbar
P3
Teil
Erholungsraum
1m
Durchreicheschleuse mit zwei druckdicht verriegelbaren Türen
Stickstoflager/ Tank/Zapfsystem
Luftdusche/ Schleuse
Behandlungsraum 2 teilig Erholungsraum
Blutaufarbeitung/Diagnostik P3 TB-Raumteil
HIVRaumteil Kryolabor S3**
Eingang Personal
Autoklav
Erste Hilfe Tisch + Liege Sauerstoffversorgung von oben, ansonsten wie in Erste Hilfe Wagen
Möglichkeit, Vorhänge dazwischen zu ziehen Eingang/Ausgang für die Patienten Geschlossene Zeltüberdachung mit ausrollbarem Bodenteil
Global HIV Vaccine Research Cryorepository – GHRC
Notausgang