GHRC - Global HIV Vaccine Research Cryorepository. Global HIV Vaccine Research Cryorepository - GHRC. Global HIV Vaccine Research Cryorepository GHRC

GHRC - Global HIV Vaccine Research Cryorepository Global HIV Vaccine Research Cryorepository - GHRC Global HIV Vaccine Research Cryorepository – GHR...
Author: Irene Logan
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GHRC - Global HIV Vaccine Research Cryorepository

Global HIV Vaccine Research Cryorepository - GHRC

Global HIV Vaccine Research Cryorepository – GHRC

CAVD – Collaboration for AIDS Vaccine Discovery •

> 165 investigators in 22 countries from 89 institutions – 16 Principal Investigators: 11 in US, 5 in Europe – Australia, Austria, Belgium, Brazil, Cameroon, Canada, China, Denmark, France, Germany, India, Japan, Netherlands, South Africa, Spain, Sweden, Switzerland, Thailand, Uganda, UK, US, and Zambia



11 Vaccine Discovery Consortia (VDCs) – 5 focused on neutralizing antibodies – 6 focused on cell mediated immunity



5 Central Service Facilities (CSFs) – 2 Vaccine immune Monitoring Centers – 1 Mouse Immunology Lab – 1 Specimen repository – 1 Statistical Center



Alliance Management Contract

Global HIV Vaccine Research Cryorepository – GHRC

Vaccine Discovery Consortia Antibody Stamatatos

Letvin

Haynes

Weiss

Lehner

Patterson

Cellular immunity Zamb

Pantaleo

Zolla-Pazner McElrath Self

Von Briesen

Koup

Montefiori Greenberg

Central Service Facilities Global HIV Vaccine Research Cryorepository – GHRC

Ho

GHRC – Major Aims

The “CAVD Consortium on Global HIV Vaccine Research Cryorepository (GHRC)“ implies the establishment of a new and unique cryorepository. ! The establishment of a CAVD specific global HIV specimen collection ! Clinical samples: plasma, PBMC, virus isolates ! Key reagents from CAVD consortia: vectors, candidate vaccines, peptides, antibodies, rec. proteins

! New strategies of sample collection (e.g. “early infections”). ! New quality of preservation technology and sample processing. ! Future-proof technology for collecting, preparing, conserving and distributing reagents for extended networks. ! Technology upgrade of primary sites and transfer of operating procedures to and exchange of specimens with other VIMCs and VDCs. Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Objectives Coordination & Management Networking, Training and Logistics

Research on HIV Specific Cryoprocedures

GHRC Development of a HIV Cryotechnology Platform

Fully Operational HIV Cryobank Evaluation of Dedicated Cryotechnologies, Tools & Procedures Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Research on HIV Specific Cryoprocedures

Primary sites

Whole blood (~40ml)

serum / plasma

PBMC

Viral RNA

virus isolates

Primary sites and/or Cryobank

Cryobank

Cryopreservation

Neutralization assays

Full length cloning

Fulllength sequencing

Full-length cloning

T-cell assays

Partner

! Optimized sample processing protocols ! Optimized cryoprotectants ! New strategies (e.g. cells on surfaces) Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Development of a HIV Cryotechnology Platform

Rack access without white frost and fog

!HIV cryotank with access tower !HIV-specific cryosubstrates !Cryogenic memory cards !Pipetting robot

Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Development of temperature and ice protection hood system "Warm" protection space 0 % humidity

Isolated cryogenic work space with temperature controlled nitrogen atmosphere

Isolated cryogenic storage space

Basis vessel Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Prototypes of HIV cryosubstrates

250 !l 500 !l

100 !l

Substrate with coupled memory device Well-Plate with electrical contacts Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Fully Operational HIV Cryobank (P3) Co-funding by Government of Saarland and FhG

Specimen processing •Cryoprocedures •Quality control •Shipment

Technology •Operation of cryotanks •Cryogenic electronic infrastructure •Shipment

Data management •Webinterface •Administration (e.g. backup) •Connection to VISC, VIMC, VDC

Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Cryo Collections

! clinical specimen collections e.g. stem cells ! backup-collections ! biological and material research specimens

Global HIV Vaccine Research Cryorepository – GHRC

GHRC

Cryo Control Room

Cryo-Security-Area

! over 14,000 sqft floorspace for cryovessel installation ! over 75,000 gallons of cryospace (at -250 F) ! today about 5,000 gallons (at –250 F) installed Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Networking, Training and Logistics

! Selection of Primary Sites (PS) ! Transfer of cryoequipment & procedures to PS ! Training workshops ! Technology transfer to VIMCs and VDCs ! Collaboration with VISC ! Reagent distribution (NIBSC, NIH)

Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Overall Milestones Selection of Primary Sites with access to early infections completed

M12

P3 laboratory ready for operation including certification

M15

Specimen-specific cryosubstrates and manual tools developed and evaluated

M24

SOPs for optimized cryopreservation of PBMCs using validated assays for measuring immune reactive T cells completed

M24

50 to 100 full-length viral clones & sequences acquired

M36

Evaluation of operational cryobank and P3 laboratory by the GHAVE Network

M36

Global HIV Vaccine Research Cryorepository – GHRC

GHRC – Coordination & Management Centralized Facility FhG-IBMT

Coordination

Evaluation

Research CryoProcedure s

Cryo Devices

Optimized Specimen Processing

UdS DIBIT

2 VIMCs MIL

11 VDCs 11 VDCs

Data Management & Central Database

Technology Platform Substrates

Low Temp Electronics

Specimens VISC

Cryobank Network and Logistics & Special Equipment Training and Capacity Building + SOPs

ULund UdS FhGIBMT

Primary Sites Cohorts: early infections (< 6 months)

Global HIV Vaccine Research Cryorepository – GHRC

NIBSC NIH WHO

GHRC – Mobile Laboratory Unit for Sample Collection, Diagnostic and Sample Processing Co-funding by FhG

Ausziehbarer

Ggf auch noch ausziehbar

P3

Teil

Erholungsraum

1m

Durchreicheschleuse mit zwei druckdicht verriegelbaren Türen

Stickstoflager/ Tank/Zapfsystem

Luftdusche/ Schleuse

Behandlungsraum 2 teilig Erholungsraum

Blutaufarbeitung/Diagnostik P3 TB-Raumteil

HIVRaumteil Kryolabor S3**

Eingang Personal

Autoklav

Erste Hilfe Tisch + Liege Sauerstoffversorgung von oben, ansonsten wie in Erste Hilfe Wagen

Möglichkeit, Vorhänge dazwischen zu ziehen Eingang/Ausgang für die Patienten Geschlossene Zeltüberdachung mit ausrollbarem Bodenteil

Global HIV Vaccine Research Cryorepository – GHRC

Notausgang

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