FROM A MICROFLUIDIC TOOLBOX TO A LAB ON A CHIP

Contact : Claude Vauchier [email protected]

MICROFLUIDIC TOOLBOX: A COMPLETE DESIGN KIT Important criteria Type of fluids (air, aqueous or organic liquid) Sample & fluid volume flow rate Simulation tools Comsol, MathLab, Evolver y x z

A complete design kit and a set of µfluidic methods to cover the full range of volumes & flow rates for air & liquids

Depending on the protocol to integrate, different kind of fluidics will be used: Monophasic Microfluidics Diphasic Microfluidics

Flow Actuators

100 µL/min

Silicon & PDMS µpumps and µvalves

Plasma separation of large blood samples for diagnostics

Flox focussing: Langerhaans ilet encapsulation

Pillar reactor: sample preparation for point of care systems

EWOD Electrowetting on Dielectrical, digital microfluidic

Smart Capilarity for textiles & bandages

LAB ON A CHIP: A COMPLEX MANUFACTURING PROCESS NEEDING A MULTIDISCIPLINARY TEAM Chemistry Microtechnology Biopackaging Biology

Silicon & glass manufacturing in clean room microtechnology facilities, PDMS and polymer molding, ….

Biocompatible assembly of Chemical grafting of several types of material surfaces for fluidic (hard and flexible, properties as well as electrical, fluidic and biological probes (DNA, thermal connections…) Antibodies, ..) for species recognition

A multidiciplinary team of 80 persons dedicated to Labs on a Chip Commissariat à l’énergie atomique et aux énergies alternatives MINATEC Campus | 17 rue des martyrs | F-38054 Grenoble Cedex Établissement public à caractère industriel et commercial | RCS Paris B 775 685 019

Biological specification and validation

Contact : Claude Vauchier

MICROFLUIDIC FOR SAMPLE PREPARATION

Critical functions of a point of care system

SAMPLE PREPARATION Consist of a set of sample treatment (concentration, purification, lysis) to enhance sensitivity and specificity of the detection steps Why use microsystems? • When scale reduction is important • Sample is small: less dilution • Small objects need to be manipulated, cells • Surface interaction needs to be improved • A real need for integration • POC analysis, environment monitoring • Quick answer with low technical setting

[email protected]

Air sampling

Immunology

Water sampling

Genomics

Human sampling

Spectroscopy (MS, Raman)

Sampling “How to collect sample and maintain its integrity“

Pre Analytics “How to transfer the analyte as fast, as concentrate and as “pure” as possible on a sensor“

Sensing “How to detect, identify and/or quantify analyses“

Signal processing / Communication / User Interface “ How to extract data and deliver the answer at the right level (number, curve, color) and the right place (on site or far away) “

Scaling down, surface forces become predominant, new mechanisms appear enabling new ways, opening new possibilities

PLASMA SEPARATION ON LARGE BLOOD SAMPLE Passive continuous flow microfluidic is used to separate blood cells from plasma, replacing ultracentrifugation Advantages: • Continuous mode: independent of volume sample • Passive system: no valves, no electrodes, no energy • Simple and robust system for delocalized application

• Plasma quality comparable to centrifugation • Extraction yield ≈ 25% of flow rate (50-200 µL/min) Plasma

100 µL/min

Blood cells

Air

Plasma Blood separation

Check valve

PDMS Lab on a chip for plasma extraction from whole blood

Pressurized air

PURIFICATION AND CONCENTRATION ON PILLAR CHIPS Perfectly ordered pillars are used as generic Sample prep for host response follow up in ICU microreactors. Molecules and polymers can be covalently grafted to their surfaces in order to have 20 min a reactor with specific molecular recognition. ARNm Blood 100µL

20 minutes

Pillars are used for filtering extracting purifying and concentrating

Photos of silicon pillar chips

Commissariat à l’énergie atomique et aux énergies alternatives MINATEC Campus | 17 rue des martyrs | F-38054 Grenoble Cedex Établissement public à caractère industriel et commercial | RCS Paris B 775 685 019

10 to 50µL

Fully integrated and automated protocol

Sample prep for drug analysis in blood

Whole blood is injected on the pillar chip, blood cells filter trough while the drug is retained and further eluted to an HPLC detector

Microfluidics opens up new ways for sample preparation

LENSFREE IMAGING OF TISSUE, CELLS, BACTERIA AND VIRUSES

Contact : Claude Vauchier [email protected]

Research is continuously developing new imaging methods to better understand the structure and function of biological systems at the microscopic scale. Despite our ability to peer through the cells using increasingly powerful optical instruments, fundamental biology questions remain unanswered at larger scales. Hence we are developing lensfree imaging as an alternative method bringing new perspectives, i.e. our system aims at • Multiscale observation capability across several orders of magnitude • Large field of view >20 mm2 • Live capture inside the incubator over several days (acquisition every 5 minutes) • Simplicity of use, small form factor (