Fourth European Congress of Virology Cernobbio, Italy April 7 – 11, 2010

Imprint Editors Prof. Gabriella Campadelli-Fiume, Bologna, Italy Prof. Carlo De Giuli Morghen, Milano, Italy Prof. Giorgio Palù, Padova, Italy Abstract Management, Layout Dr. Arianna Calistri, Padova, Italy Dr. Cristiano Salata, Padova, Italy MZ Congressi, Milano, Italy Cover Layout MZ Congressi, Milano, Italy Printed by Associazione Padre Monti, Saronno (VA), Italy

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Table of Contents

Table of Contents Committees and Welcome

pages 5 – 8

Awards

pages 9 – 11

Partecipating Organizations and Patronages

page 12

Business Meetings

page 13

Sponsor and Exhibitors

pages 14 – 17

General Information

pages 18 – 20

Programme at a glance

page 21

Special Workshops

pages 22 – 25

Schedule of poster sessions

page 26

Scientific Programme

pages 27 – 63

Keynote Speakers’ Abstracts

pages 64 – 77

Oral Presentations’ Abstracts

pages 78 – 127

Poster Presentations’ Abstracts

pages 128 – 305

Author index

pages 306 – 323

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Committees

Convenors Gabriella Campadelli-Fiume (Bologna) Carlo De Giuli Morghen (Milano) Giorgio Palù (Padova)

Local Executive Committee Arianna Calistri (Padova) Francesco Cancellotti (Padova) Antonina Dolei (Sassari) Laura Menotti (Bologna) Cristina Parolin (Padova) Antonia Radaelli (Milano) Cristiano Salata (Padova)

Scientific Committee Mathias Ackermann (Switzerland)

Jeremy Luban (Switzerland)

Ralf Bartenschlager (Germany)

Åke Lundkvist (Sweden)

Ben Berkhout (The Netherlands)

Penelope Mavromara (Greece)

William F. Carman (United Kingdom)

Thomas Mertens (Germany)

Karl-Klaus Conzelmann (Germany)

Janos Minarovits (Hungary)

Manfred P. Dierich (Austria)

Darius Moradpour (Switzerland)

Antonina Dolei (Italy)

Claude P. Muller (Luxemburg)

Esteban Domingo (Spain)

Nikolaus Mueller-Lantzsch (Germany)

Richard M. Elliott (United Kingdom)

Albert D.M.E. Osterhaus (The Nederlands)

Mariano Esteban (Spain)

Ernst Peterhans (Switzerland)

Bernhard Fleckenstein (Germany)

Elisabeth Puchhammer-Stöckl (Austria)

Giuseppe Gerna (Italy)

Bracha Rager (Israel)

Rob. W. Goldbach (The Netherlands)

Félix A. Rey (France)

Vladimir Gurtsevitch (Russia)

Kalle Saksela (Finland)

Otto Haller (Germany)

Thomas Schulz (Germany)

Franz X. Heinz (Austria)

Stuart Siddel (United Kingdom)

Ari Helenius (Switzerland)

Geoffrey L. Smith (United Kingdom)

William L. Irving (United Kingdom)

Jan Svoboda (Czech Republic)

Marion Koopmans (The Nederlands)

Noël Tordo (France)

Ulrich Koszinowski (Germany)

Goran Wadell (Sweden)

Hans-Georg Kräusslich (Germany)

Luc Willems (Belgium)

Jacomine Krijnse Locker (Germany)

Hans Wolf (Germany)

Bruno Lina (France)

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List of Symposia and Keynote Speakers

Symposia and Keynote Speakers Sergio Abrignani (Italy) Neil Almond (United Kingdom) Charles R.M. Bangham (United Kingdom) Ralf Bartenschlager (Germany) Roberto Cattaneo (USA) Yuan Chang (USA) Karl-Klaus Conzelmann (Germany) Peter V. Coyle (United Kingdom) Ennio De Gregorio (Italy) Esteban Domingo (Spain) Lynn W. Enquist (USA) Bernhard Fleckenstein (Germany) Ricardo Flores (Spain) Peter Ghazal (United Kingdom) Paul Griffiths (United Kingdom) Otto Haller (Germany) Mark Harris (United Kingdom) Franz X. Heinz (Austria) Gunilla Karlsson Hedestam (Sweden) Yoshihiro Kawaoka (Japan) Frank Kirchhoff (Germany) Marion Koopmans (The Nederlands) Ulrich Koszinowski (Germany) Antonio Lanzavecchia (Switzerland) Carlo Liverani (Italy) Jeremy Luban (Switzerland) Thomas C. Mettenleiter (Germany) Luigi Naldini (Italy) Hubert G.M. Niesters (The Nederlands) Hiroyuki Ogata (France) Juan Ortin (Spain) Albert D.M.E. Osterhaus (The Nederlands) Peter Palese (USA) Massimo Palmarini (United Kingdom) Lucas Pelkmans (Switzerland) Sébastien Pfeffer (France) Herbert Pfister (Germany) Daniel D. Pinschewer (Switzerland) Maria G. Revello (Italy) Félix A. Rey (France) Cristina Risco (Spain) Bernard Roizman (USA) Rob W. H. Ruigrok (France) Rozanne M. Sandri-Goldin (USA) Tino F. Schwarz (Germany) John J. Skehel (United Kingdom) Geoffrey L. Smith (United Kingdom) Margaret A. Stanley (United Kingdom) Michael Taliansky (Russia) Nöel Tordo (France) Peter Vogt (USA) Richard J. Whitley (USA) Ian Wilson (USA) Alessandro Zanetti (Italy) Jessica Zucman-Rossi (France)

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List of Chairpersons

Chairpersons Geoffrey L. Smith (United Kingdom) Thomas Stamminger (Germany) Lennart Svensson (Sweden) Nöel Tordo (France) Richard J. Whitley (USA) Dana Wolf (Israel) Hans Wolf (Germany) Alessandro Zanetti (Italy)

Sergio Abrignani (Italy) Antonio Alcami (Spain) Jan Balzarini (Belgium) Charles R. M. Bangham (United Kingdom) Ben Berkhout (the Netherlands) Wolfram Brune (Germany) Franco Buonaguro (Italy) Gabriella Campadelli-Fiume (Italy) Francesco Cancellotti (Italy) Maria R. Capobianchi (Italy) Roberto Cattaneo (USA) Karl-Klaus Conzelmann (Germany) François-Loïc Cosset (France) Peter V. Coyle (United Kingdom) Carlo De Giuli Morghen (Italy) Antonina Dolei (Italy) Esteban Domingo (Spain) Isabella Donatelli (Italy) Luis Enjuanes (Spain) Lynn W. Enquist (USA) Bernhard Fleckenstein (Germany) Ricardo Flores (Spain) Giuseppe Gerna (Italy) Peter Ghazal (United Kingdom) Urs F. Greber (Switzerland) Paul Griffiths (United Kingdom) Otto Haller (Germany) Mark Harris (United Kingdom) Franz X. Heinz (Austria) Hartmut Hengel (Germany) Gunilla Karlsson Hedestam (Sweden) Frank Kirchhoff (Germany) Marion Koopmans (The Nederlands) Santo Landolfo (Italy) Bruno Lina (France) Jeremy Luban (Switzerland) Roberto Manservigi (Italy) Giovanni P. Martelli (Italy) Penelope Mavromara (Greece) Thomas Mertens (Germany) Thomas C. Mettenleiter (Germany) Nikolaus Mueller-Lantzsch (Germany) Hubert G.M. Niesters (The Nederlands) Juan Ortin (Spain) Albert D.M.E. Osterhaus (The Nederlands) Massimo Palmarini (United Kingdom) Giorgio Palù (Italy) Cristina Parolin (Italy) Sébastien Pfeffer (France) Mauro Pistello (Italy) Elisabeth Puchhammer-Stöckl (Austria) Maria G. Revello (Italy) Giovanni Rezza (Italy) Cristina Risco (Spain) Peter J.M. Rottier (The Nederlands) Franco M. Ruggeri (Italy) Kalle Saksela (Finland) Rozanne M. Sandri-Goldin (USA) Thomas Schultz (Germany) Tino F. Schwarz (Germany)

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Welcome

Welcome to the Fourth European Congress of Virology Dear Colleagues, It is our great pleasure to welcome you to the 4th European Congress of Virology (ECV) in Cernobbio, lake of Como, IT, April 7-11, 2010 (www.eurovirology2010.org). This congress will represent the annual meeting of different Scientific Societies, namely the European Society for Clinical Virology, the German/Swiss/Austrian Society for Virology (Gesellschaft fϋr Virologie) and the Italian Society for Virology. The Eurovirology Congresses, renamed European Virology Congresses, were initiated in 2000 to bring together young and senior investigators from all Countries of Europe and from all branches of Virology. The aim was to strengthen Virology in Europe, by providing a platform for basic, medical, clinical, veterinary, and plant Virology. Congresses were held sequentially in UK, Spain and Germany. Importantly, they have fostered the foundation of the European Society for Virology (www.eusv.eu), the inaugural meeting of which was held in Rome, 24th of April, 2009. The venue is now the beautiful “Spazio Congressi Villa Erba”, in Cernobbio, Lake of Como, a worldwide famous international resort. Cernobbio is one of the most enchanting Italian corners. Very close to Como and around 50 Km away from the Malpensa Airport, the main airport in Northern Italy, Cernobbio dominates the west side of the lake, offering breathtaking views of it and a charming atmosphere. Together, with advice from the Scientific Advisory Committee, we have endeavored to put together an exciting and informative programme that covers the many facets of Virology. We sincerely thank you for your participation and we hope that you will find the 4th European Virology Congress and the location stimulating and enjoyable.

Gabriella Campadelli-Fiume (Convenor)

Carlo De Giuli Morghen (Convenor)

Giorgio Palù (Convenor)

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Awards

European Virology Award (EVA) Sunday, April 11 2010, 10.05-10.50, Room A Awardee: P. Palese, Mount Sinai School of Medicine, USA Award Lecture: “Pandemic Influenza viruses: Past and Future”

Gardner Lectureship awarded by European Society for Clinical Virology Wednesday, April 7 2010, 18.00-18.30, Room A Awardee: Y. Kawaoka, University of Wisconsin School of Veterinary Medicine, USA Award Lecture: “2009 Pandemic Influenza”

Loeffler-Frosch Medal donated by Gesellschaft für Virologie (GfV) Saturday, April 10 2010, 12.00-12.30, Room A Awardee: P. Vogt, The Scripps Research Institute, USA Award Lecture: “From Rous sarcoma to cancer genomics: the emergence of therapeutic targets”

Loeffler-Frosch Lecture and Prize awarded by Gesellschaft für Virologie (GfV) Sunday, April 11 2010, 8.30-9.00, Room A Awardee: D. Pinschewer, University of Geneva, Switzerland Award Lecture: “Arenaviruses: immunity, immunopathology and vaccines”

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Awards

SIV Pioneer in Virology Lecture and Prize awarded by Italian Society for Virology (SIV) Saturday, April 10 2010, 12.30-13.00, Room A Awardee: U. Koszinowski, Ludwig-Maximilians Universität, Germany Award Lecture: Pygmalion dreams while moulding CMV

Abbott Award by European Society for Clinical Virology (ESCV) Friday, April 9 2010, 14.00-16.30, Room D Awardee: K. Van Laethem, Katholieke Universiteit Leuven, Belgium Award Lecture: “HIV-1 gp41 mutations 43T and 50V elevate resistance levels of common enfuvirtide mutants while all mutants remained highly susceptible to sifuvirtide”

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Young Scientists Travel Awards

Young scientists travel awards 4th European Congress of Virology Ana Vázquez, Juan Francisco Garcia Arriaza, Jura Inga Henke, Florian Amelingmeier, Anna K. Överby, Nina Wolfrum, Julia Bitzegeio

European Society for Virology (ESV) Christine Goffinet, Geoffrey Chase, Krzysztof Pyrc, Sanela Svraka

European Society for Clinical Virology (ESCV) Nicola Page, Joana Rocha-Pereira, Thijs van der Laar, Sabine van der Sanden, Xinling Xu, Alison Watt, Rui Qi Xue, Ivana La Zarevic, Nektaria Goulidaki, Anupam Mukherjee

Federation of European Microbiological Societies (FEMS) Joana Abrantes, Benedikt Asbach, Cecilia Bender, Alessandra de Leo, Adriaan de Wilde, Valentina Gatta, Janine Kimpel, Andrea Karin Kress, Kevin Maringer, Awad Ali Shehata, Niki Vassilaki, Hila Winer

Gesellschaft für Virologie (GfV) Carina Banning, Katharina Böckl, Daniel Bourquain, Patricia Fliß, Silke Germann, Henrik Groß, Eva-Maria Haendel, Herwig Koppensteiner, Andreas Muschaweckh, Corinna Pietsch, Martina Rieder, Bastian Thaa, Peter Witkowski, Kerstin Wunderlich

Italian Society for Virology (SIV) Licia Bordi, Cinzia Borgogna, Angela Chiereghin, Roberto Delogu, Emanuela Noris, Annacarmen Petrizzo, Elena Sartori, Marco Turci, Luigi Bertolotti, Alberto Alberti, Massimiliano Pagani, Sabrina Benedetti

Society for General Microbiology (SGM) Mhairi Ferguson, Eleanor Gaunt

Spanish Society of Virology (SEV) Selma Gago, Alberto López-Bueno, Adrian Valli, Sonia Zúñiga

Swedish Society for Virology Marie Hagbom, Rickard Nordén

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Participating Organizations and Patronages

The Following Organizations Take Part in the Congress European Society for Virology (ESV) European Society for Clinical Virology (ESCV) Federation of European Microbiological Societies (FEMS) Gesellschaft für Virologie (GfV) Italian Society for Virology (SIV) Society for General Microbiology American Society for Microbiology

Association Journées Francophones de Virologie Danish Society for Virology European Society for Veterinary Virology Hellenic Society of Virology International Society for Neurovirology Netherlands Society for Microbiology Norwegian Society for Virology Society for General Microbiology Spanish Society of Virology Swedish Society for Microbiology Swedish Molecular Virology Network Swiss Society for Microbiology

Patronages DIPARTIMENTO DI ISTOLOGIA, MICROBIOLOGIA E BIOTECNOLOGIE MEDICHE

PROVINCIA DI COMO Assessorato Servizi Sociali, Sanità e Pari Opportunità

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Business Meetings of the European Organizations

Business Meetings of the European Organizations Thursday, April 8, 2010 19.00-20.00, GfV General Membership Assembly, Room A 19.00-20.00, ESCV Council Meeting, Room B 19.00-20.00, SIV General Assembly, Room C Friday, April 9, 2010 18.30-20.30, ESV General Assembly, Room A

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Exhibitors

The industrial support has been essential for the organization of the 4th European Congress of Virology (ECV). We wish to acknowledge the following Companies for their participation in the exhibition and other means of sponsoring and support.

Gold Sponsors

Silver Sponsors

Sponsors

ARGENE BD BIOSCIENCES BIOSITE INTERNATIONAL COPAN ITALIA DIESSE DIAGNOSTICA SENESE EUROCLONE EUROGENTEC GILEAD SCIENCES CULLIGAN

INVITEK MILTENYI BIOTEC MP BIOMEDICALS NOVARTIS PHARMA PROMEGA QIAGEN ROCHE PHARMA VETERINARY RESEARCH

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Facilities for the 4th European Congress of Virology

“Spazio Villa Erba” Facilities for the 4th European Congress of Virology

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Exhibitors

Exhibitors List (see the map in the next page) Exhibitor

Booth number

ABBOTT ARGENE COPAN ITALIA DIASORIN DIESSE DIAGNOSTICA SENESE EUROGENTEC INVITEK LUMINEX B.V. MERCK SHARP & DOHME MP BIOMEDICALS NANOGEN PROMEGA ITALIA QIAGEN ROCHE DIAGNOSTICS SIEMENS HEALTHCARE DIAGNOSTICS

1 14 9 16 10 11 4 13 8 12 2 3 6 15 5

American Society for Microbiology (ASM) European Society for Virology (ESV) European Society of Clinical Virology (ESCV) Federation of European Microbiological Socieies (FEMS) Gesellschaft für Virologie (GfV) Italian Society for Virology (SIV)

A B C D E F

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Exhibitor Area, Cernobbio Wing

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General Information

General Information Onsite registrations will be accepted and must be paid by credit card or cash only.

Organizing Secretariat MZ Congressi s.r.l. Via Carlo Farini 81 - 20159 Milano (Italy) Phone: +39 02-66802323 – Fax: +39 02-6686699 E-mail: [email protected]

Lunch tickets are available: 1-day ticket: 14 euro 3-days ticket: 40 euro

MIZAR Conventions (Official accommodation agent for 4th European Congress of Virology) Via 5 Giornate 2/R 22012 Cernobbio (Como) – Italy Phone: +39 031-342025 - Fax: +39 31-342015 E-mail: [email protected]

The registration fees include: Entrance to plenary symposia, workshops, poster sessions, exhibition and industry-sponsored workshops Programme/abstract book congress documents Ferryboat (Como - Congress Centre - Como) in the morning and at the end of the sessions Coffee/tea during morning and afternoon breaks Get together, wine and cheese, Wednesday, April 7, 19.00-20.30

Venue Spazio Villa Erba Largo Luchino Visconti, 422012 Cernobbio (CO) Italy http://www.villaerba.it/VillaErba_en.html

Oral presentations The congress language is English. The symposia and workshops will take place in the lecture halls “A”, “B”, “C” and “D”. All the rooms are equipped with projectors for Power Point presentations and laptops. All presentations must be submitted as Windows compatible Power Point files on PC readable Cds, DVDs, external disk drivers, USB sticks, memory sticks or ZIP. Personal laptops cannot be accepted. If you need a Mac, please contact us in advance. You are requested to hand your presentation at the media check the day before your session, and, for the first day, at least 60 minutes prior to the start of the session. You will find the media-check in the “Slide Center”, located near Room D (see “Spazio Villa Erba Facilities for the 4th European Congress of Virology” paragraph). Time allowed for oral presentation is limited to 11 minutes, plus 2 minutes for discussion

Congress homepage www.eurovirology2010.org

Registration Counter (during the meeting) Phone: +39 031-349376 Fax: +39 031-349375 Wednesday, April 7, 2010 Thursday, April 8, 2010 Friday, April 9, 2010 Saturday, April 10, 2010 Sunday, April 11, 2010

14.00-19.00 8.00-18.30 8.30-18.30 8.30-18.30 8.30-13.00

Registration and Name Badge Please check in the registration counter (at the entrance of the congress area, see “Spazio Villa Erba Facilities for the 4th European Congress of Virology” paragraph) on arrival. There you will receive your congress documents and your name badge. Your personal name badge must be worn at all time, because only registered participants will be allowed to enter the congress area. Moreover, the badge is necessary for shuttle service (with ferryboat).

Posters The poster exhibition is situated in the Congress Center, near the registration counter (see “Spazio Villa Erba Facilities for the 4th European Congress of Virology” paragraph). Please place your poster at the board reserved for you, referring to your assigned Poster Code. The boards are 80 cm wide by 100 cm high. Poster numbers are indicated on the top of each board. It is possible to hand out written material related to your poster, but this material should not be left overnight. Posters must be on display from April 8 to April 11. Authors are required to attend the poster area during the appropriate Poster Session. You must withdraw your poster before 1.00 pm on April 11. Posters not retrieved by this time will be discarded. Information presented at the 4th ECV or contained in the Scientific Programme and Abstract book should not be cited as a publication. This material can be

Before December 31, 2009 Full Registration € 500,00 (VAT included) Young Registration* € 150,00 (VAT included) Day Registration € 180,00 (VAT included) After December 31, 2009 Full Registration € 650,00 (VAT included) Young Registration* € 220,00 (VAT included) Day Registration € 180,00 (VAT included) * Reduced fee for Undergraduate/PhD students (proof of student status is required)

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General Information cited as a personal communication only after the consent of the senior author has been obtained. Photographing, audiotaping, or videotaping of presentations by audience members is not permitted.

Internet - WiFi Several computers with internet connection will be available in the “Internet room” (see “Spazio Villa Erba Facilities for the 4th European Congress of Virology” paragraph). Free Wi-Fi connection will be also available in Cernobbio Wing.

Continuous Medical Education A request of accreditation for CME points has been submitted to the European Accreditation Council. The credit points will be announced on the Congress website as soon as they are available. You will receive your certificate upon request at the Registration Desk. The 4th ECV will be accredited also for Italian participants according to Ministry of Health rules.

Opening Hours of the Industrial Exhibition The Exhibition Area will be open as follows: Wednesday, April 7, 2010 15.00-19.00 Thursday, April 8, 2010 08.30-18.30 Friday, April 9, 2010 08.30-18.30 Saturday, April 10, 2010 08.30-18.30 Sunday, April 11, 2010 08.30-13.00

How to reach the Congress Centre By car From Milan take the A9 Milan-Chiasso highway; take the COMO Nord exit (the last exit for Italy); then follow the signs for Villa Erba. Our multi-storey car park is 200metres from the Conference Centre. From Lugano take the Lugano-Milan highway; take the CERNOBBIO Lago exit 200 metres after Customs.

By train Ferrovie Nord Milano (www.ferrovienord.it) Departures from Piazza Cadorna, Milan, train for COMO Lago; alight at the last stop, COMO Lago. Outside the station is the bus stop for line CC6, COMOMaslianico; alight at Villa Erba (www.sptlinea.it, phone: 031-247111). The trip is about 4 km. Ferrovie dello Stato (www.trenitalia.com) Departures from Milano Centrale or from Milano Porta Garibaldi, destination Chiasso, alight at COMO San Giovanni. Outside the station is the bus stop for line CC6, COMO-Maslianico; alight at Villa Erba (www.sptlinea.it, phone: 031-247111). The trip is about 5 km.

Airport Connections

Social Dinner The Social Dinner will take place on Saturday, April 10, 2010 at 8:00 pm, at the Grand Hotel of Como (website www.grandhoteldicomo.it), located at walking distance from the Congress Venue. The menu is based on the typical and renowned Italian gastronomy, and it will be accompanied by a selection of local wines. A vegetarian menu will be also available. Shuttle service will not be provided.

Interesting links about Como and its Lake http://www.comune.como.it/como/eng/ http://www.lakecomo.org/eng/index_eng.asp http://www.comolake.com/index.php?lang=en_EN

Aeroporto di Milano Linate (www.sea-aeroportimilano.it), around 50 km away. Aeroporto di Milano Malpensa (www.sea-aeroportimilano.it), around 35 km away. From Malpensa it is possible to take the Malpensa Express train to Saronno; change here for the Ferrovie Nord Milano train for COMO Lago. Outside the station is the bus stop for line CC6, COMO-Maslianico; alight at Villa Erba (www.sptlinea.it, phone: 031-247111). The journey is around 4 km. Alternatively a bus service connects the airport to COMO Lago, run by SPT (www.sptlinea.it, phone: 031-247111). Timetable for departures to COMO: 9.20 - 11.00 - 14.15 - 16.45 19.25, daily service.

Taxi Taxi COMO, Stazione S. Giovanni, Piazzale S. Gottardo – phone: 031-271000 - Toll-free phone 800.012380 Radio Taxi Lario 2000, Via Anzani 52 – phone: 0312772 Taxi-Radio, v.le Innocenzo XI 70 – phone: 031-261515

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General Information Ferryboat departure from Como

Congress Parking facility A car parking facility with fee (cost 10 euro per day) will be available close to the congress centre.

Airport shuttle service and transportation A shuttle service from/to Milan (Malpensa, Linate) and Bergamo (Orio al Serio) Airports will be provided on April 7 and April 11, 2010.

Ferryboat transportation Como-CernobbioComo A free shuttle service between “Como Hotels / Congress Centre / Como Hotels" will be provided during the congress, in the morning and in the evening, concomitant with beginning and end of sessions. Hereafter the schedule of the service: FROM COMO TO CERNOBBIO Wednesday, 7 April Shuttles at: 14:30 – 14:45 – 15:00 – 15:15 – 15:30 – 15:45 – 16:00 – 16:15 – 16:30 From Thursday 8 to Sunday 11 April Shuttles at: 8:00 – 8:15 – 8:30 – 8:45 – 9:00 – 9:15 FROM CERNOBBIO TO COMO Wednesday, 7 April Shuttles at: 19:00 – 19:15 – 19:30 – 19:45 – 20:00 – 20:15 – 20:30 – 20:45 – 21:00 From Thursday 8 to Saturday 10 April Shuttles at: 18:00 – 18:15 – 18:30 – 18:45 – 19:00 – 19:15 – 19:30 – 19:45 – 20:00 Sunday, 11 April Shuttles at: 11:30 – 11:45 – 12:00 – 12:15 – 12:30 – 12:45 – 13:00 – 13:15 – 13:30 The final schedule and pick up point will be posted in the hotel lobbies and at the congress centre as well. In addition please find hereafter the schedule for the public ferryboats: FROM COMO TO CERNOBBIO Wednesday, 7 April 14:45 – 15:15 – 15:45 – 16:15 – 16:30 From Thursday 8 to Saturday 10 April 7:35 – 7:50 – 8:15 – 8:45 Sunday, 11 April 8:45 – 9:15 – 9:20 FROM CERNOBBIO TO COMO Wednesday, 7 April 20:10 – 20:35 From Thursday 8 to Saturday 10 April 17:30 – 18:23 – 18:25 – 18:46 – 18:55 – 19:31 Sunday, 11 April 12:56 – 13:14 – 13:44

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Further details for the public ferryboat and bus service at the following link: http://www.navigazionelaghi.it/eng/com/nlc.html http://www.eurovirology2010.org/pdf/busLine6.pdf

Programme at a Glance

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Special Workshops

Special Workshops Thursday, April 8, 2010 16.45-18.45

Novartis Vaccines and Diagnostics Workshop Emerging Viral Infections

Chair:

C. W. Mandl (USA)

Keynote Lectures:

N. Nowotny (Austria) Emergence and dispersal of West Nile and Usutu virus infections in Europe G. Del Giudice (Italy) The shaping of the immune response to pandemic influenza vaccines by adjuvants S. Modrow (Germany) Human parvoviruses: New looks on old friends M. Lecuit (France) Chikungunya: from bedside to bench, and back

16.45-18.45

AIDS: from a deadly to a treatable, chronic disease. Where the research has made the difference

Chair:

C. F. Perno (Italy), R. Kaiser (Germany)

Keynote Lectures:

A. Antinori (Italy) From a fatal to a chronic disease: how therapy is changing rules and fate D. Pillay (United Kingdom) Phylogenesis of HIV and worldwide viral spread F. Ceccherini-Silberstein (Italy) Regulation of HIV replication by secondary structure of genomic RNA J. Balzarini (Belgium) Glycosylation of HIV proteins and cellular receptors: from basic science to clinical implication V. Calvez (France) Resistance to new antiviral drugs. How it happens, how to avoid it R. Paredes (Spain) Diagnostic and therapeutic approach to HIV tropism

16.45-17.45

Diasorin Workshop New insights into parvovirus B19: A diagnostic perspective

Chairs:

K. Hedman (Finland), G. Palù (Italy)

Keynote Lectures:

P.V. Coyle (United Kingdom) Parvovirus B19 Infection - Can we do better? G. Gallinella (Italy) Development and relevance of diagnostic assays for parvovirus B19 Infections F. Bonelli (Italy) Behind the scene: the development of diagnostic tests

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Room A

Room B

Room C

Special Workshops 17.45-18.45

Luminex / Abbott Molecular Respiratory Disease Workshop Current and future perspectives in clinical diagnosis of viral respiratory pathogens using a unique xTAG multiplexing technology

Room C

Chair:

F. Topin (The Netherlands)

Keynote Lectures:

M. Gorgievski-Hrisoho (Switzerland) Clinical utility of multiplex respiratory viral target testing in immunocompromised patients R. Ortiz de Lejarazu (Spain) Utility of the respiratory viral diagnosis using the xTAG RVP assay during the flu pandemy

16.45-17.45

Nanogen Advanced Diagnostics Workshop Cytomegalovirus Infections/Diseases in Transplant Patients

Chair:

P. Griffiths (United Kingdom), T. Lazzarotto (Italy)

Keynote Lectures:

P. Grossi (Italy) Clinical management and therapeutic options T. Lazzarotto (Italy) Updated guidelines for the diagnosis of CMV in solid organ transplantation G. Stefanuto (Italy) ELITe MGB: advancement in molecular biology diagnostics

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Room D

Special Workshops

Friday, April 9, 2010 16.30-18.30

Prevention of HPV related diseases

Chair:

G. Palù (Italy)

Keynote Lectures:

M. A. Stanley (United Kingdom) Biological mechanism of HPV infection P. Bonanni (Italy) Primary prevention: state of the art in Europe E. Joura (Austria) New frontiers for quadrivalent HPV L. Barzon (Italy) Secondary prevention in the vaccine era

16.30-18.00

Abbott Workshop Room C Continuous innovation in ID by complementary use of different technologies: Serology, NAT and beyond

Chair:

G. A. Vockel (Germany)

Keynote Lectures:

A. Alberti (Italy) Monitoring of chronic HCV and HBV infections D. Prati (Italy) HCV Ag - a sensible adjunct in HCV diagnostics? J. Hackett (USA) The history of HIV diagnostic assays M. Eshoo (USA) PLEX-ID - Unique and innovative technology for unsolved problems in virology

16.30-18.30

New diagnostic and prognostic tools for the clinician

Chair:

R. Bartenschlager (Germany)

Keynote Lectures:

M. Colombo (Italy) HCV therapy : standard of care 2010 S. Zeuzem (Germany) New Interferons and immunotherapies for HCV D. Olsen (USA) A future without interferon A. Alberti (Italy) Natural history and determinants of disease outcomes

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Room B

Room D

Special Workshops

Saturday, April 10, 2010 16.45-17.45

Roche Workshop Molecular diagnostics for prognostic assessment and therapeutic drug monitoring of HCV and HIV infections

Chair:

G. Palù (Italy)

Keynote Lectures:

M. Schutten (The Netherlands) Real-time PCR based viral load assays and the increasing complexity of HIV patients management P. Halfon (France) HCV viremia monitoring in response guided therapies L. Barzon (Italy) Micro RNA and HCV

16.45-18.45

Control of Novel H1N1 Influenza

Chair:

E. Mocarski (USA)

Keynote Lecture:

R. Donis (USA) Challenges in the 2009 H1N1 Pandemic Response H. Jin (USA) 2009 H1N1 Live Attenuated Influenza Vaccine P. Wilson (USA) Antibody Prophylaxis of H1N1 Influenza K. Iishi (Japan) Plasmacytoid DCs in Influenza Vaccine Response

16.45-18.45

Hepatitis B : From Laboratory to Clinic

Chair:

G. Carosi (Italy)

Keynote Lectures:

G. Fattovich (Italy) Natural history and treatment F. Bonino (Italy) Monitoring response during antiviral therapy S. Locarnini (Australia) HBV drug resistance in 2010 M. Rizzetto (Italy) HDV: the old becomes new

25

Room B

Room C

Room D

Schedule of the Poster Sessions

Schedule of the Poster Sessions Thursday - April 8, 2010, 16.45-19.00, Poster Area Workshop Poster Code • Alpha- and Beta-herpesviruses replication and genetics, Herpesviruses – Oncogenesis and signaling 340-399 • DNA viruses 317-339 • Hepatitis viruses 273-316 • Oncolytic viruses and Gene Therapy 685-708 • Silencing of gene expression 563-574 • Systems biology of viral replication, Virus-host interactions, Virus-host interplay 469-562 • Viral glycoproteins and entry, Viral glycoproteins and receptors 426-468 • Virus structure, assembly and exit 400-425

Friday - April 9, 2010, 16.45-19.00, Poster Area Workshop • Antiviral and Resistance • Diagnostics and Molecular Typing • Emerging viruses and zoonoses • Epidemiology and Evolution • Relevant clinical infections: viral gastroenteritis and parvovirus B19 • Viral infections in pregnancy, Viral infections in transplant recipients

Poster Code 791-844 726-790 087-121 001-035 067-086 036-066

Saturday - April 10, 2010, 16.45-19.00, Poster Area Workshop • Adaptive immunity and immune prevention • Endogenous retroviruses and neurovirology • HIV • Intrinsic and innate immunity and pathogenesis • Plant viruses • Influenza viruses • Retroviruses • RNA viruses, Negative stranded viruses • Viral persistence and immune evasion

Poster Code 628-669 709-725 223-272 594-627 575-593 122-174 212-222 175-211 670-684

26

Scientific Programme – Wednesday, April 7, 2010

Wednesday, April 7, 2010 15.45-16.15

Opening ceremony

Room A

Welcome address Local organizers: G. Campadelli-Fiume, C. De Giuli Morghen, G. Palù Italian political authorities: from Ministry of Research and Education and Ministry of Health Scientific representatives: O. Haller (ESV), L. W. Enquist (ASM), R. M. Sandri-Goldin (ASV), B. Lina (ESCV), M. Da Costa (FEMS), V. Taleski (FEMS)

16.15-19.00

Opening session

Room A

16.15-17.45 Chair:

Challenges in vaccine development and innate immunity G. Campadelli-Fiume, C. De Giuli Morghen, G. Palù

Keynote Lectures:

O. Haller Innate Immunity to Influenza Viruses E. De Gregorio Molecular and cellular signatures of human vaccine adjuvants I. Wilson Structure-assisted vaccine design for influenza virus and HIV-1

17.45-18.15

European Society for Clinical Virology - Gardner Lecture:

Awardee:

Y. Kawaoka 2009 Pandemic Influenza

18.15-19.00

Redefining Virology

Keynote Lectures:

H. Ogata Mimivirus and other giants of the virus world L. W. Enquist Viral genomes are part of the phylogenetic tree of life

19.00-20.30

Get together, wine and cheese

27

Scientific Programme – Thursday, April 8, 2010

Thursday, April 8, 2010 08.30-10.30

Hepatitis C Virus Roundtable

Room A

Chair:

S. Abrignani

Keynote Lectures:

M. Harris Enhanced hepatitis C genome replication and lipid accumulation mediated by inhibition of AMP-activated protein kinase in hepatitis C virus infected cells S. Abrignani Mirnome analyses of human sera reveals biomarkers of HCV disease progression A. Lanzavecchia Dissecting the human immune response to pathogens and vaccines J. Zucman-Rossi Molecular classification of HCCs: new insights and interest in clinical practice

08.30-10.30

W1 - Virus-host interplay

Chairs:

L. W. Enquist, H. Hengel

Keynote Lecture: 08.30-09.00

L. W. Enquist (ASM Workshop) Mechanisms of spread of herpesvirus infection in peripheral nervous system neurons

09.00-10.30

Oral presentations

Room B

KSHV INDUCES ENDOTHELIAL TO MESENCHYMAL TRANSITION IN A 3DIMENSIONAL CELL MODEL S. Laurinavicius (1), F. Cheng.(1), N. Sugiyama (2), K. Alitalo (2), K. Lehti (2) and P.M. Ojala (1) (1) Genome-Scale Biology Program, Biomedicum Helsinki & Institute of Biomedicine, University of Helsinki, (2) Molecular Cancer Biology Program, Biomedicum Helsinki & Haartman Institute, University of Helsinki, Finland TRANSPORT INTO AND OUT OF THE NUCLEUS IN HERPES SIMPLEX VIRUSINFECTED CELLS P. Malik (1,2), A. Tabarraei (2) and R.H. Kehlenbach (3) (1) Wellcome Trust Centre For Cell Biology, ICB, King's Building, University of Edinburgh, Edinburgh, United Kingdom EH9 3JR, (2) IBLS, Division of Infection and Immunity, University of Glasgow, 120 University Place, Glasgow, United Kingdom G12 8TA and (3) Department of Biochemistry, University of Gottingen, Gottingen, Germany ERM-PROTEIN DEPENDENT MODULATION OF THE ACTIN CYTOSKELETON BY THE EPSTEIN-BARR VIRUS ENCODED PROTEIN COMPLEX BDLF2/BMRF2 J. Mühe, J. Deerberg, K. Ritter and M. Kleines Division of Virology, Department of Medical Microbiology, UK Aachen, Aachen, Germany RETROVIRUS BUDDING INDUCED REMODELING OF THE HOST CELL ACTIN ARCHITECTURE M. Gladnikoff (1), E. Shimoni (2), N.S. Gov (3) and I. Rousso (1) (1) Department of Structural Biology, (2) Electron Microscopy Center, and (3) Department of Chemical Physics, Weizmann Institute of Science, Rehovot

28

Scientific Programme – Thursday, April 8, 2010 PARVOVIRUS INDUCED ALTERATIONS IN NUCLEAR ARCHITECTURE AND DYNAMICS T.O. Ihalainen (1), EA Niskanen (1), N. Dross (2), J. Langowski (2), J. Timonen (3) and M Vihinen-Ranta (1) (1) NanoScience Center; Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland, (2) Division Biophysics of Macromolecules, German Cancer Research Center (DKFZ), Heidelberg, Germany and (3) Department of Physics, University of Jyväskylä, Jyväskylä, Finland A HUMAN LUNG EXPLANT MODEL TO STUDY INFECTION BY HUMAN, AVIAN AND PORCINE INFLUENZA A VIRUSES V. K. Weinheimer (1), A. Becher (2), M. Matthaei1, T. Bauer (3), M. Tönnies (3), A. Hocke (2), S. Hippenstiel (2) and T. Wolff (1) (1) Div. of Influenza/Respiratory Viruses, Robert Koch-Institut, Berlin, Germany, (2) Medizinische Klinik m. S. Infektiologie Charité, Berlin, Germany and (3) HELIOS Klinikum Emil von Behring, Berlin, Germany

08.30-10.30

W2 - Intrinsic and innate immunity and pathogenesis

Chairs:

J. Luban, T. Stamminger

Keynote Lecture: 08.30-09.00

J. Luban TRIM5 and Innate Immunity to HIV-1

09.00-10.30

Oral presentations

Room C

NF-κB IN SARS-CORONAVIRUS INFECTION B. L. Haagmans, S. L. Smits, A. de Lang, J.M.A. van den Brand, L. M.E. Leijten, A. Andeweg, G. van Amerongen, T. Kuiken and A.D.M.E. Osterhaus Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands MOLECULAR BASIS OF SARS-CoV VIRULENCE AND PROTECTION M. L. DeDiego (1), J. Netland (2), E. Álvarez (1), J. L. Nieto-Torres (1), S. Perlman (2) and L. Enjuanes (1) (1) Department of Molecular and Cell Biology. Centro Nacional de Biotecnologia (CNB), CSIC. Darwin 3. Campus Universidad Autonoma. 28049 Madrid. Spain and (2) University of Iowa, Iowa 52242. STRUCTURAL INSIGHTS INTO THE ANTIVIRAL MxA PROTEIN: IMPORTANCE OF MxA OLIGOMERISATION FOR ITS FUNCTION A. von der Malsburg (1), S. Gao (2), S. Paeschke (2), J. Behlke (2), O. Daumke (2), G. Kochs (1) and O. Haller (1) (1) Department of Virology, Institute of Medical Mikrobiology, University of Freiburg, Freiburg, Germany and (2) Crystallography Department, Max-Delbrück-Centre for Molecular Medicine, Berlin, Germany REGULATION BY INTERFERONS OF VIRALLY-INDUCED PATHOLOGY INVOLVING MACROPHAGES J.P. Coutelier, D. Su, T. Le-Thi-Phuong, G. Thirion and L. Detalle De Duve Institute, Universitá Catholique De Louvain, Brussels, Belgium

INDIRECT

THE ROLE OF THE PDZ RECOGNITION DOMAIN IN THE VIRAL NS1 PROTEIN OF HIGHLY PATHOGENIC AVIAN INFLUENZA A VIRUS F. Zielecki (1), I. Semmler (1), D. Kalthoff (2), M. Beer (2) and T. Wolff (1) (1) Div. of Influenza/Respiratory Viruses; Robert Koch-Institut, Berlin, Germany and (2) Institute of Diagnostic Virology; Friedrich-Loeffler-Institut, Insel Riems, Germany

29

Scientific Programme – Thursday, April 8, 2010 DECIPHERING DEGRADATION-DEPENDENT AND -INDEPENDENT MODES OF CD317 ANTAGONISM BY HIV-1 VPU C. Goffinet, S. Homann, N. Tibroni, I. Allespach, O.T. Keppler and O.T. Fackler Department of Infectious Diseases, Virology, University of Heidelberg, Germany

08.30-10.30

W3 - Epidemiology and evolution

Chairs:

E. Domingo, G. Rezza

Keynote Lecture: 08.30-09.00

E. Domingo Mechanism of lethal mutagenesis, and implications for antiviral design

09.00-10.30

Oral presentations

Room D

MICROEVOLUTION OF PUUMALA HANTAVIRUS A. Plyusnin Department of Virology, Haartman Institute, University of Helsinki, Finland EPIDEMIOLOGY AND MOLECULAR CHARACTERIZATION OF WEST NILE VIRUS INFECTION IN NORTH-EASTERN ITALY L. Barzon (1,2), E. Franchin (1,2), L. Squarzon (1,2), E. Lavezzo (1), S. Toppo (3), T. Martello (1), S. Bressan (1), S. Pagni (1,2), M. Cattai (2), A. Piazza (2), M. Pacenti (2), R. Cusinato (2) and G. Palù (1,2) (1) Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, Padua, Italy, (2) Regional Reference Centre for Infectious Diseases, Microbiology and Virology Unit, Azienda Ospedaliera di Padova, Padua, Italy and (3) Department of Biological Chemistry, University of Padova, Padua, Italy. WASTEWATER SURVEILLANCE REVEALS WIDESPREAD CIRCULATION OF NOVEL HUMAN PICORNAVIRUSES, INCLUDING HUMAN COSAVIRUSES AND CADIOVIRUSES, KLASSEVIRUS AND A PUTATIVE NEW PICORNAVIRUS GENUS J. Calvert., E.C. McWilliam-Leitch and P. Simmonds Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh, EH9 1QH, UK INSIGHTS INTO HUMAN RHINOVIRUS EVOLUTION IN INOCULATED IMMUNOCOMPETENT SUBJECTS S. Cordey (1), C. Tapparel (1), T. Junier (2), D. Gerlach (2), B. Winther (3), L. Farinelli (4), F. Gobbini (1) and L. Kaiser (1). (1) Laboratory of Virology, Division of Infectious Diseases, University of Geneva Hospitals, Rue Gabrielle-Perret-Gentil 4, 1211 Geneva 14, Switzerland, (2) Department of Genetic Medicine and Development, University of Geneva Medical School, 1 rue Michel-Servet, 1211 Geneva 14, Switzerland, (3) Division of General Pediatrics, Department of Pediatrics, University of Virginia, Charlottesville, USA and (4) Fasteris SA, CH-1228 Plan-les-Ouates, Switzerland. HUMAN HERPESVIRUS TYPE 8 VARIANTS CIRCULATING IN EUROPE, AFRICA AND NORTH-AMERICA IN ASSOCIATION WITH CLASSIC, ENDEMIC AND EPIDEMIC KAPOSI'S SARCOMA DURING PRE-AIDS AND AIDS ERA M.L. Tornesello (1), B. Biryahwaho (2), R. Downing (2), A. Hatzakis (3), E. Alessi (4), M. Cusini (4), V. Ruocco (5), G. Loquercio (1), E. Katongole-Mbidde (2), L. Buonaguro (1) and F.M. Buonaguro (1) (1) Molecular Biology and Viral Oncology and AIDS Ref. Centre, National Cancer Institute "Fond. Pascale", Naples, Italy, (2) Uganda Virus Research Institute, PO Box 49 Entebbe, Uganda, (3) University of Athens, Athens, Greece, (4) Institute of Dermatological Sciences, University and IRCCS Ospedale Maggiore of Milan, Italy and (5) Department of Dermatology, Second University of Naples, Naples, Italy.

30

Scientific Programme – Thursday, April 8, 2010 HIGH DIVERSITY OF THE VIRAL COMMUNITY FROM AN ANTARCTIC LAKE A. López-Bueno (1), J. Tamames (2), D. Velazquez (3), A. Moya (2), A. Quesada (3) and A. Alcamí (1) (1) Centro de Biología Molecular “Severo Ochoa” (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid, Spain, (2) Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, València, Spain and (3) Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain. 10.30-11.00

Coffee Break

11.00-13.00

Plenary session: Virus structure and entry

Chairs:

G. Campadelli-Fiume, O. Haller

Keynote Lectures:

F. A. Rey X-ray structures of the surface glycoprotein complex of chikungunya virus: insight into alphavirus particle assembly and allosteric activation for entry R.W.H. Ruigrok Structure and function of the influenza virus polymerase complex L. Pelkmans Using cell population context to study virus infection J. Skehel Receptor Recognition by Pandemic Influenza Viruses

12.45-13.00

European Commission Research Representative C. Desaintes EU funded research on viral infectious diseases

13.00-14.00

Break

14.00-16.30

W4 - Virus-host interactions

Chairs:

R. M. Sandri-Goldin, T. Enjuanes

Keynote Lecture: 14.00-14.30

R. M. Sandri-Goldin The Multifunctional Protein ICP27 Is a Pivotal Player during Herpes Simplex Virus Infection

14.30-16.30

Oral presentations

Room A

Room A

A UNIQUE STATE OF LYTIC HCMV CHROMATIN MAINTAINED BY THE VIRAL IE1 PROTEIN M. Nevels, A. Nitzsche, C. Winterling, K. Mücke and C. Paulus. Institute for Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany. INFLUENZA VIRUS REQUIREMENTS FOR EIF4F COMPONENTS: HIJACKING CELLULAR TRANSLATION MACHINERY E. Yángüez (1), A. Castelló (2), I.G. Goodfellow (3) and A. Nieto (1) (1) Centro Nacional de Biotecnología (C.S.I.C.) and Ciber de Enfermedades Infecciosas. Darwin 3, Campus de Cantoblanco, 28049, Madrid, Spain, (2) Centro de Biología Molecular Severo Ochoa (C.S.I.C.) Nicolás Cabrera 1, Campus de la Universidad Autónoma de Madrid, 28049, Madrid and (3) Department of Virology, Faculty of Medicine, Imperial College London, St Mary's Campus, Norfolk Place, London W2 1PG, UK

31

Scientific Programme – Thursday, April 8, 2010 NUCLEOLIN INTERACTS DIRECTLY WITH Us11 PROTEIN OF HERPES SIMPLEX VIRUS TYPE 1 A. Greco (1), E. Soler (1), Y. Couté (3,4), L. Arata (1), S. Hacot (1), A. Callé (1), J.C. Sanchez (3), P. Bouvet (2) and J.J. Diaz (1) (1) Centre de Génétique Moléculaire et Cellulaire, CNRS, UMR5534, 16 rue Dubois, Villeurbanne, F-69622, France, (2) Laboratoire Joliot-Curie, Ecole Normale Supérieure de Lyon, CNRS USR 3010, 46 Allée d'Italie, Lyon, France, (3) Geneva Proteomics Center, Central Clinical Chemistry Laboratory, Rue Micheli-du-Crest 24, 1211 Genève, Switzerland and (4) Present address: EDyP-Service, INSERM 0880 / UJF, CEA / DSV / iRTSV / LEDyP / C3-313E, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France MOLECULAR CHARACTERIZATION OF CRMD, THE TNF AND CHEMOKINE DECOY RECEPTOR OF POXVIRUS S.M. Pontejo (1), M.B. Ruiz-Argüello (2) and A. Alcami (1) (1) Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, 28049 Madrid, Spain and (2) Centro de investigación en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain. THE ELK-1 AND SERUM RESPONSE FACTOR BINDING SITES IN THE MAJOR IMMEDIATE-EARLY PROMOTER OF THE HUMAN CYTOMEGALOVIRUS ARE REQUIRED FOR EFFICIENT VIRAL REPLICATION IN QUIESCENT CELLS P. Caposio, A. Luganini, M. Bronzini, S. Landolfo and G. Gribaudo Department of Public Health and Microbiology, University of Turin, Turin, Italy EPSTEIN-BARR VIRUS EBNA1 DISRUPTS PML NUCLEAR BODIES IN EBVASSOCIATE EPITHELIAL TUMOURS N. Sivachandran (1), L. S. Young (2), C.W. Lawson (2), J. Middeldorp (3) and L. Frappier (1) (1) Department Of Molecular Genetics, University of Toronto, Toronto, Canada; (2) University of Birmingham, Birmingham, UK and (3) Vrije Universiteit Medical Center, Amsterdam, The Netherlands HAPLOID GENETIC SCREENS IN HUMAN CELLS IDENTIFY HOST FACTORS USED BY PATHOGENS J.E. Carette, C. Guimaraes, M. Varadarajan, E. Spooner, A. Park, A. Godarova, H.L. Ploegh And T.R. Brummelkamp Whitehead Institute For Biomedical Research, Nine Cambridge Center, Cambridge, MA, 02142, USA CELLULAR RESPONSE TO MEASLES VIRUS INFECTION: APOPTOSIS OR AUTOPHAGY L. Fragnet (1,2), C.K. Pfaller (1) and K.-K. Conzelmann (1) (1) Max von Pettenkofer-Institute & Gene Center, LMU Munich, Germany and (2) INRA IASP-213, Tours, France.

14.00-16.30

W5 - Influenza Viruses

Room B

Chairs:

J. Ortín, I. Donatelli

Keynote Lecture: 14.00-14.30

J. Ortín Structural and functional analyses of influenza virus RNA replication

32

Scientific Programme – Thursday, April 8, 2010 14.30-16.30

Oral presentations ADAPTIVE MUTATIONS IN THE POLYMERASE OF TWO HUMAN H5N1 ISOLATES RESULT IN DIFFERENT PREFERENCES TOWARDS TRANSCRIPTION OR REPLICATION, RESPECTIVELY B. Mänz (1,2), D. Mayer (1), H.-D. Klenk (2) and M. Schwemmle (1) (1) Department of Virology, University Freiburg, Germany and (2) Institute for Virology, University Marburg, Germany FITNESS OF PANDEMIC H1N1 AND SEASONAL INFLUENZA A VIRUSES DURING CO-INFECTION E. Sorrell, M. Angel, J. Ye, D. Hickman, L. Pena, G. Ramirez-Nieto, B. Kimble, Y. Araya and D. Perez Department of Veterinary Medicine, University of Maryland, College Park and VirginiaMaryland Regional College of Veterinary Medicine, College Park, Maryland, USA PROTECTIVE EFFICACY OF MESSENGER RNA-BASED VACCINES AGAINST LETHAL INFLUENZA A INFECTION IN MICE B. Petsch*(1), M. Schnee*(2), K. Neumann (2), K-J. Kallen (2), T. Kramps (2) and L. Stitz (1) (* equal contribution) (1) Institute for Immunology, Friedrich-Loeffler-Institute and (2) CureVac GmbH, Tübingen, Germany INFLUENZA VIRION MORPHOLOGIES EXPLAINED BY CELLULAR ORIGIN, SUB-TYPES AND GENETIC BACKGROUND: IMPLICATIONS FOR VACCINE DESIGN AND PATHOGENESIS STUDIES V. Moulès (1), O. Terrier (1), M. Yver (1), M. Guidice (2), O. Ferraris (1), D. Thomas (2), G. Schoehn (3) and B. Lina (1) (1) Université Lyon 1, CNRS FRE 3011 VirPath, France and (2) Université Rennes 1, CNRS UMR6026, France, 3UMI 3265 UJF-EMBL-CNRS, France CONTRIBUTION OF THE INFLUENZA A VIRUS POLYMERASE COMPLEX TO HIGH VIRULENCE IN MICE P. Zimmermann (1), T. Rolling (1), N. Robb (2), E. Fodor (2), O. Haller (1), P. Stäheli (1) and G. Kochs (1) (1) Department of Virology, University of Freiburg and (2) Sir William Dunn School of Pathology, Oxford A ROLE OF INFLUENZA A VIRUS NON-STRUCTURAL PROTEIN 1 (NS1) IN VIRAL mRNA EXPORT? M. Franz and T. Wolff Div. of Influenza/Respiratory Viruses, Robert Koch-Institut, Berlin, Germany. PROTECTIVE ROLE FOR PROTEASE-ACTIVATED-RECEPTOR-2 AGAINST INFLUENZA VIRUS PATHOGENESIS VIA AN INTERFERON-GAMMADEPENDENT PATHWAY K. Khoufache, F. LeBouder, E. Morello, F. Laurent, S. Riffault, P. Andrade-Gordon, S. Boullier, P. Rousset, N. Vergnolle and B. Riteau Unité de Virologie et Immunologie Moléculaires, UR 892 INRA, Domaine de Vilvert, 78352 Jouy-en-Josas, France INFLUENZA A VIRUS RIBONUCLEOPROTEIN COMPLEXES FORM DIFFERENT SPECIES IN INFECTED NUCLEI BY INTERACTING WITH SUBNUCLEAR STRUCTURES AND NUCLEAR PROTEINS G. Chase (1), MA. Rameix-Welti (2), N. Naffakh (2) and M. Schwemmle (1) (1) Department of Virology, University of Freiburg, Freiburg, Germany and (2) Institut Pasteur, Paris, France

33

Scientific Programme – Thursday, April 8, 2010 14.00-16.30

W6 - HIV

Room C

Chairs:

F. Kirchhoff, B. Berkhout

Keynote Lecture: 14.00-14.30

F. Kirchhoff Role of Nef and Vpu in HIV and SIV pathogenesis and transmission

14.30-16.30

Oral presentations STRIKING CHANGES OF miRNAS EXPRESSION IN CD4+ T LYMPHOCYTES OCCURRED EVEN IN THE ABSENCE OF AN ESTABLISHED HIV-1 INFECTION F. Bignami (1), E. Pilotti (2), L. Bertoncelli (3), P. Ronzi (1), M. Gulli (4), N. Marmiroli (4), G. Magnani (5), L. Lopalco (6), R. Ruotolo (7), M. Galli (1), A. Cossarizza (3) and C. Casoli (1) (1) Dipartimento di Scienze Cliniche, Sezione di Malattie Infettive e di Immunopatologia, Università degli Studi di Milano, (2) Spazio Salute Immigrati, AUSL Parma, (3) Dipartimento di Scienze Biomediche, Università degli Studi di Modena e Reggio Emilia, Modena, (4) Dipartimento di Scienze Ambientali, Università degli Studi di Parma, (5) U.O. di Malattie Infettive, Arcispedale S. Maria Nuova, Reggio Emilia, (6) Divisione di Immunologia, Malattie Infettive e Trapianti, Fondazione Centro San Raffaele, Milano and (7) Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Italy THE NEF PROTEIN OF HIV-1 ENHANCES VIRAL TRANSCRIPTION BY INTERFERING WITH THE RECRUITMENT OF IKKα TO THE HIV PROMOTER T. Haase (1), P. Gintschel (1), A. S. Baur (2) and V. Witte (1) (1) Institute for Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany and (2) Department of Dermatology, Friedrich-Alexander University, Erlangen, Germany ENDOCYTOSIS OF HIV-1 INTO PLASMACYTOID DENDRITIC CELLS K. Pritschet (1), N. Donhauser (1), M. Ries (1), S. Haupt (1), P. Schuster (1), N.A. Kittan (1,2), K. Korn (1), S. Pöhlmann (3), N. Bannert (4), E. Bogner (5) and B. Schmidt (1) (1) Institute of Clinical and Molecular Virology, German National Reference Centre for Retroviruses, University of Erlangen-Nürnberg, (2) University Hospital Regensburg, Regensburg, (3) Institute of Virology, Hannover Medical School, Hannover and (4) Robert Koch-Institute, Berlin and (5) Institute of Virology, Charité University Hospital, Berlin, Germany EXCISION OF HIV-1 PROVIRAL DNA USING TRE-RECOMBINASE: AN EXPERIMENTAL UPDATE C.H. Nagel (1), J. Chemnitz (1), I. Hauber (1), P. Ziegler (2), H. Hofmann-Sieber (1), A. Schambach (3), C. Baum (3), M. Manz (4), F. Buchholz (5) and J. Hauber (1) (1) Heinrich-Pette-Institute For Experimental Virology And Immunology, Martinistrasse 52, 20251 Hamburg, Germany, (2) Institute For Research In Biomedicine, Via Vincenzo Vela 6, 6500 Bellinzona, Switzerland, (3) Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany, (4) University Hospital Zurich, Raemistrassse 100, 8091 Zurich, Switzerland and (5) Max-Planck-Institute For Molecular Cell Biology And Genetics, Pfotenhauer Strasse 108, 01307 Dresden, Germany LIVE ATTENUATED SIV: CHARACTERISING THE ROLE OF VACCINE PERSISTENCE IN PROTECTION B. Berkhout Laboratory of Experimental Virology, University of Amsterdam, The Netherlands HIV-1 VIF BINDS TO APOBEC3G mRNA AND INHIBITS ITS TRANSLATION G. Mercenne, S. Bernacchi, D. Richer, G. Bec, S. Henriet, J.-C. Paillart and R. Marquet Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, 67084 Strasbourg, France

34

Scientific Programme – Thursday, April 8, 2010 DISLOCATION OF THE FRAMESHIFT SIGNAL UNCOUPLES GAG AND POL READING FRAMES AND REVEALS THE ROLE OF THE TRANSFRAME PROTEIN P6* IN HIV-1 REPLICATION A. Leiherer , C. Ludwig and R. Wagner. Molecular Microbiology and Gene Therapy Unit, Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany STRONG VIRUS-SPECIFIC MUCOSAL IMMUNE RESPONSES IN SIV-INFECTED RHESUS MONKEYS SIGNIFICANTLY CONTRIBUTE TO VIRAL CONTAINMENT AND LONG-TERM SURVIVAL T. Schultheiss, R. Schulte, U. Sauermann and C. Stahl-Hennig Department of Infection Models, German Primate Center, Goettingen, Germany 14.00-16.30

W7 - Antivirals and resistance

Chairs:

R. J. Whitley, N. Tordo

Keynote Lecture: 14.00-14.30

R. J. Whitley Recent Advances in Antiviral Therapy (Non-AIDS): Efficacy versus Resistance

14.30-16.30

Oral presentations

Room D

DEPLETION OF GTP BY RIBAVIRIN IS NOT THE PREDOMINANT MECHANISM OF ACTION AGAINST LASSA VIRUS S. Ölschläger (1), J. Neyts (2) and S. Günther (1) (1) Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany and (2) Rega Institute for Medical Research, Leuven, Belgium FREQUENCIES OF HEPATITIS B SURFACE-ANTIGEN MUTATIONS IN DRUG RESISTANT HEPATITIS B VIRUS ISOLATES M. Fraune (1), N. Sichtig (1), B. Beggel (2), F Wilhelm (1), M. Obermeier (3), T. Berg (3), T. Lengauer (2), D. Glebe (4), R. Kaiser (1) and J. Verheyen (1) (1) Institute of Virology, University of Cologne, (2) Max Planck Institute for Informatics, Saarbrücken, (3) Medizinisches Labor Berg, Berlin and (4) Institute of Medical Virology, Justus-Liebig University of Gießen, Germany PRECLINICAL STUDIES ON MYRCLUDEX B, A NOVEL ENTRY INHIBITOR FOR HEPATITS B (HBV) AND HEPATITIS DELTA VIRUS (HDV) INFECTIONS S. Urban (1), A. Schulze (1), A. Schieck (2), C. Gähler (1), Y. Ni (1), A. Meier (1), A. Alexandrov (3) and W. Mier (2) (1) Department Of Infectious Diseases, Molecular Virology, University Clinics Heidelberg, Heidelberg, Germany, (2) Department Of Nuclear Medicine, University Clinics Heidelberg, Heidelberg, Germany and (3) Vision 7 GmbH, Frankfurt/Main, Germany

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Scientific Programme – Thursday, April 8, 2010 EFFICACY AND SAFETY OF OSELTAMIVIR-ZANAMIVIR COMBINATION COMPARED TO EACH MONOTHERAPY FOR SEASONAL INFLUENZA: A RANDOMIZED, DOUBLE-BLINDED, PLACEBO-CONTROLLED TRIAL M. Bouscambert Duchamp (1,2), T. Blanchon (3,4), F. Mentré (5,6,7), V. Enouf (8), F. Tubach (9,6,7), A. Mosnier (10), B. Lina (1,2), Q. Dornic (5,6,7), C. Leport (11,12) and S. van der Werf (8,13,14) (1) Hospices Civils de Lyon, Centre National de Référence des virus influenzae (RégionSud), GHE, Bron, France, (2) Université Lyon 1, VirPatH, CNRS FRE 3011, Lyon, France, (3) Inserm UPMC UMR-S 707, Faculté de médecine Pierre et Marie Curie, Paris, France, (4) Université Pierre et Marie Curie, Paris 6, UFR de Médecine, U707, Paris, France, (5) APHP, Hôpital Bichat, UF de Biostatistiques, Paris, France, (6) Inserm U738, Paris, France, (7) Université Paris Diderot, Paris 7, UFR de Médecine, site Bichat, Paris, France, (8) Institut Pasteur, Centre National de Référence des virus influenzae (Région-Nord), Unité de Génétique Moléculaire des Virus à ARN, Paris, France, (9) APHP- Hôpital Bichat, Département d’Epidémiologie, Biostatistiques et Recherche Clinique, Paris, France, (10) Réseau des GROG, Coordination nationale, Paris, France, (11) Université Paris Diderot, Paris7, UFR de Médecine, site Bichat, Laboratoire de Recherche en Pathologie Infectieuse, Paris, France, (12) AP-HP, UF Coordination des Risques Epidémiques et Biologiques, Paris, France, (13) CNRS URA3015, Paris, France and (14) Université Paris Diderot, Paris 7, UFR Sciences du Vivant, Paris, France. NOVEL ANTIVIRAL COMPOUNDS THAT TARGET THE INFLUENZA VIRUS REPLICATION COMPLEX H.-Heinrich Hoffmann (1), Peter Palese (1, 2) and Megan L. Shaw (1) (1) Departments of Microbiology and Medicine and (2) Mount Sinai School of Medicine, New York, NY 10029 POTENTIAL BENEFITS OF SEQUENTIAL INHIBITOR-MUTAGEN TREATMENTS OF RNA VIRUS INFECTIONS C. Perales (1,2), R. Agudo (1), H. Tejero (3), S. C. Manrubia (4) and E. Domingo (1,2) (1) Departamento de Virología y Microbiología, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Consejo Superior de Investigaciones Científicas (CSIC), Campus de Cantoblanco, Madrid, Spain, (2) Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain, (3) Departamento de Bioquímica y Biología Molecular, Universidad Complutense de Madrid, Madrid, Spain and (4) Centro de Astrobiología (CSIC-INTA), Torrejón de Ardoz, Madrid, Spain. DISRUPTION OF PROTEIN-PROTEIN INTERACTIONS AS A NOVEL ANTIVIRAL STRATEGY: FROM HERPESVIRUSES TO INFLUENZA E. Sinigalia, B. Mercorelli, G. Muratore, S. Pagni, R. Cinetto, G. Palù and A. Loregian Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy AIC246: A NOVEL NON-NUCLEOSIDIC COMPOUND WITH ACTIVITY AGAINST HUMAN CYTOMEGALOVIRUS (CMV) - OVERVIEW ON CLINICAL TRIALS AND RESISTANCE BREAKING ACTIVITY H. Zimmermann (1), S. Stoelben (1), L. Renders (2), K. Budde (3), W. Arns (4), D. Michel (5), P. Lischka (1), M. Rangaraju (1) and H. Rübsamen-Schaeff (1) (1) AiCuris, Wuppertal, Germany, (2) University of Kiel, Germany, (3) University Hospital Charite, Berlin, Germany, (4) University Hospital Ulm, Germany and (5) Merheim Medical Center, Cologne, Germany 16.30-17.00

Coffee Break

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Scientific Programme – Friday, April 9, 2010

Friday, April 9, 2010 08.30-10.30

W8 - Viral glycoproteins and receptors

Chairs:

F. X. Heinz, F.L. Cosset,

Keynote Lecture: 08.30-09.00

F. X. Heinz Molecular mechanisms of flavivirus membrane fusion

09.00-10.30

Oral presentations

Room A

THREE DIMENSIONAL STRUCTURE OF BLUETONGUE VIRUS AND ITS IMPLICATIONS IN VIRUS ENTRY P. Roy Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E7HT, UK CD209 (DC-SIGN) MEDIATES INFECTION OF HUMAN DENDRITIC CELLS BY THE TWO BUNYAVIRIDAE MEMBERS UUKUNIEMI AND RIFT VALLEY FEVER VIRUSES P.Y. Lozach (1), R. Meier (1), D. Bitto (1), A. Kuehbacher (1), M. Bouloy (2) and A. Helenius (1) (1) ETH Zurich, Institute of Biochemistry, Zurich, Switzerland and (2) Pasteur Institute, Department of Virology, Paris, France THE EPHRIN RECEPTOR TYROSINE KINASE A2 IS A CELLULAR RECEPTOR FOR KAPOSI’S SARCOMA-ASSOCIATED HERPESVIRUS A. Hahn (1), J. Kaufmann (1), E. Wies (1), K. Schmidt (1), E. Naschberger, M. Stürzl, S. König (3), B. Fleckenstein (1) and F. Neipel (1) (1) Virologisches Institut, Universitätsklinikum Erlangen, Schloßgarten 4, D-91054 Erlangen, Germany, (2) Chirurgische Klinik am Universitätsklinikum Erlangen, Krankenhausstraße 12 91054 Erlangen, Germany and (3) Integrated Functional Genomics, Interdisciplinary Center of Clinical Research, University of Münster, Münster, Germany STRUCTURAL INSIGHTS INTO REOVIRUS TROPISM AND CARBOHYDRATE RECEPTOR BINDING D.M. Reiter (1), J.M. Frierson (2), T.S. Dermody (2,3) and T. Stehle (1,3) (1) Interfaculty Institute for Biochemistry, University of Tübingen, Tübingen, Germany, (2) Department of Microbiology and Immunology and (3) Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, United States of America INTEGRIN- AND CHOLESTEROL DEPENDENT INFECTION OF HeLa CELLS WITH HUMAN RHINOVIRUS 16 M. Loetzerich, C. Neugebauer, A. Jurgeit and U.F. Greber University of Zurich, Institute of Zoology, Department of Cell Biology HERPES SIMPLEX VIRUS GLYCOPROTEINS H/L BIND TO CELLS AND INHIBIT VIRUS ENTRY INDEPENDENTLY OF B3-INTEGRINS, AND THEIR CONSTITUTIVE EXPRESSION RESTRICTS INFECTION T. Gianni (1), A. Cerretani (1), R. DuBois (2), F. Rey (2) and G. Campadelli-Fiume (1) (1) Department of Experimental Pathology, Section on Microbiology and Virology, Alma Mater Studiorum – University of Bologna, Italy and (2) Unité de Virologie Structurale, Institut Pasteur, and Centre National de la Recherche Scientifique, Paris, France.

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Scientific Programme – Friday, April 9, 2010 08.30-10.30

W9 - DNA Viruses

Room B

Chairs:

G. L. Smith, F. Buonaguro

Keynote Lectures:

G. L. Smith Repulsion of super-infecting virions: A mechanism for rapid virus spread M. A. Stanley HPV not just the cause of cervical cancer

09.30-10.30

Oral presentations CONTROL OF VIRAL MRNA EXPORT BY ADENOVIRUS REGULATORY PROTEINS M. Schmid (1), O.Koyuncu (2), P. Groitl (1) and T. Dobner (1) (1) Department Of Molecular Virology, Heinrich-Pette-Institute For Experimental Virology And Immunology, Hamburg, Germany and (2) Department Of Molecular Biology, Princeton University, USA NOVEL ADENOVIRUSES IN WILD AND CAPTIVE PRIMATES: HIGH GENETIC DIVERSITY AND EVIDENCE FOR ZOONOTIC TRANSMISSIONS D.Wevers (1), F. Leendertz (2), C. Boesch (3) and B. Ehlers (1) (1) Project Group 1 "Novel Infectious Agents", Robert Koch Institut, D-13353 Berlin, Germany ; (2) Research Group "Emerging Zoonoses", Robert Koch Institut, D-13353 Berlin, Germany and (3) Max Planck Institute For Evolutionary Anthropology, Department of Primatology, Deutscher Platz 6, D-04103 Leipzig, Germany IDENTIFICATION OF CELLULAR INTERACTION PARTNERS OF THE POXVIRAL HOST-RANGE FACTOR P28 D.R. Bourquain and A. Nitsche Centre For Biological Safety 1, Robert Koch Institute, Berlin, Germany A REPORTER ASSAY FOR ANALYSIS OF HPV-16 LATE GENE EXPRESSION B. Orrú (1), F. Ryan (1) and S. Schwartz (1,2) (1) School of Biological Sciences, Dublin Institute Of Technology, Dublin and (2) Dept. of Medical Biochemistry And Microbiology, Uppsala University, Sweden.

08.30-10.30

W10 - Relevant clinical infections: viral gastroenteritis and parvovirus B19

Chairs:

M. Koopmans, P. V. Coyle

Keynote Lectures:

M. Koopmans Race against the clock: the molecular epidemiology of noroviruses P. V. Coyle Fetal loss following Parvovirus B19 infection - how big is the problem and are the public health measures in Europe adequate to prevent it

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Room C

Scientific Programme – Friday, April 9, 2010 09.30-10.30

Oral presentations MOLECULAR EPIDEMIOLOGY OF ROTAVIRUS IN ITALY, 2007-2009. SECOND REPORT OF THE ITALIAN ROTANET SURVEILLANCE PROGRAM F.M. Ruggeri (1), T. Petouchoff (1), R. Delogu (1), L. Fiore (1), O. Tcheremenskaia (1), S. De Petris (1), M. G. Pompa (2), G. Napoletano (3), F. Zanella (4), F. Russo (4), M.C. Medici (5), C. Chezzi (5), M.L. Tanzi (5), A. Raglio (6), A. Goglio (6), C. Zotti (7), A. Mignacca (8), V. Martella (9), M. Chironna (9), M. Santantonio (9), N. Comodo(10), C. Lorini (10), F. Festini (11), A. Iorio (12), G.M. Giammanco (13), S. De Grazia (13), M. D'Errico (14), A. Marigliano (14), D. Menichelli (15), C. Russo (15), G. Danzi (16), M. De Rosa (17), P.D. Mammì (18) and F. Ansaldi (19) (1) Istituto Superiore di Sanità, Rome, (2) Ministero del Lavoro, Salute e Politiche Sociali, Rome, (3) ULSS 20, Verona, (4) Regione Veneto, Venezia, (5) University of Parma, (6) Ospedali Riuniti di Bergamo, (7) University of Torino, (8) ASL Torino 5, Chieri, (9) University of Bari, (10) Universiy of Firenze, (11) Ospedale A. Meyer, Firenze, (12) University of Perugia, (13) University of Palermo, (14) University of Ancona, (15) Ospedale Pediatrico Bambino Gesù, Rome, (16) Ospedale San G. Moscati, Aversa, (17) Ospedali Riuniti Reggio Calabria, (18) ASL di Locri and (19) University of Genova, Italy A SELECTION-FREE REVERSE GENETICS SYSTEM FOR ROTAVIRUSES BASED ON THE PREFERENTIAL PACKAGING OF REARRANGED GENOMIC RNA SEGMENTS C. Troupin (1), A. Dehée (1,2), A. Schnuriger (1,2), D. Poncet (3), P. Vende (3) and A. Garbarg-Chenon (1,2) (1) ER-7 University Pierre Et Marie Curie, Paris 6, Paris, France; (2) Laboratoire De Virologie, Hospital Armand Trousseau, AP-HP, Paris, France and (3) UMR CNRS 2472INRA 1157, Gif Sur Yvette, France IMPACT OF PARVOVIRUS B19 INFECTION ON PEDIATRIC PATIENTS WITH HEMATOLOGIC AND/OR ONCOLOGIC DISORDERS R. Jitschin (1), O. Peters (2), A. Plentz (1), P. Turowski (2), H. Segerer (2) and S. Modrow (1) (1) Institut für Medizinische Mikrobiologie Und Hygiene, Universität Regensburg, FranzJosef-Strauß-Allee 11, 93053 Regensburg; Germany and (2) Klinik für Kinder- Und Jugendmedizin, Lehrstuhl Der Universitat Regensburg, Klinik St. Hedwig, Krankenhaus Der Barmherzigen Brüder, Steinmetzstr. 1-3, 93049 Regensburg; Germany MOLECULAR ROTAVIRUS SURVEILLANCE IN GERMANY: IMPLICATIONS TO SUBCLASSIFICATION AND GENOTYPING OF G8 C. Pietsch and U.G. Liebert. Institute of Virology, Leipzig University, Leipzig, Germany.

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Scientific Programme – Friday, April 9, 2010 08.30-10.30

W11 - Hepatitis viruses

Chairs:

M. Harris, P. Mavromara

08.30-10.30

Oral presentations

Room D

AN INFECTION-BASED siRNA SCREEN TARGETING THE HUMAN KINOME IDENTIFIES KINASES INVOLVED IN THE PHOSPHOINOSITIDE METABOLISM AS KEY PLAYERS FOR HEPATITIS C VIRUS REPLICATION. I. Wörz (1), S. Reiss (1), H. Erfle (2), P. Matula (3), L. Kaderali (4), M. Pönisch (1), M. Albrecht (5), H. Blankenburg (5), S. Diehl (5), F. Ramirez (5), K. Rohr (3), A. Kaul (6), S. Bühler (1), R. Pepperkok (7), V. Lohmann (1) and R. Bartenschlager (1) (1) Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany, (2) ViroQuant CellNetworks RNAi Screening Facility, University of Heidelberg, Heidelberg, Germany, (3) Department of Bioinformatics and Functional Genomics, BIOQUANT, IPMB and DKFZ, University of Heidelberg, Heidelberg, Germany, (4) ViroQuant Research Group Modeling, University of Heidelberg, Heidelberg, Germany, (5) Max Planck Institute for Informatics, Saarbrücken, Germany, (6) ViroLogik GmbH, Erlangen, Germany and (7) EMBL, Heidelberg, Germany DEFECTIVE INTERFERING VIRAL PARTICLES AS A RISK FACTOR FOR DISEASE PROGRESSION IN PATIENTS WITH HEPATITIS C VIRUS INFECTION L. Donnici (1), A. Bianco (1), S. Noppornpanth (1), M. Boca (1), V. Valveri (1), F. Bonino (2), M. Brunetto (2), S. Abrignani (1) and R. De Francesco (1) (1) Istituto Nazionale Genetica Molecolare (INGM), Milano and (2) Dept. of Gastroenterology and Hepatology, Azienda Ospedaliera Pisana, Cisanello, Pisa ALIX IS ESSENTIAL FOR HEPATITIS B VIRUS NAKED CAPSID BUDDING A. Bardens, J. Stieler, T. Döring and R. Prange Department of Medical Microbiology and Hygiene, Universitätsmedizin der Johannes Gutenberg-Universität Mainz, D-55131 Mainz, Germany HEPATITS B VIRUS INFECTS PRIMARY HEPATOCYTES VIA A CLATHRIN-/ CAVEOLIN AND DYNAMIN- INDEPENDENT ENDOCYTIC PATHWAY N. Kott, A. König and D. Glebe Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen, Germany OXYGEN TENSION MODULATES HEPATITIS C VIRUS PROLIFERATION N. Vassilaki (1), K.I. Kalliampakou (1), I. Kotta-Loizou (1), D. Smirlis (2), R. Bartenschlager (3) and P. Mavromara (1) (1) Molecular Virology Laboratory, Hellenic Pasteur Institute (HPI), Athens, Greece, (2) Microbiology, HPI, Athens, Greece and (3) Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany. HEPATITIS C VIRUS HYPERVARIABLE REGION 1 MODULATES RECEPTOR INTERACTIONS, CONCEALS THE CD81 BINDING SITE AND PROTECTS CONSERVED NEUTRALIZATION EPITOPES D. Bankwitz (1), E. Steinmann (1), J. Bitzegeio (1), S. Ciesek (1,2), E. Hermann (3), M. B. Zeisel (4), T. F. Baumert (4), Z. Keck (5), S. K.H. Foung (5), E.-I. Pécheur (6) and T. Pietschmann (1) (1) Division of Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany, (2) Department of Gastroenterology, Hepatology and Endocrinology, Medical School Hannover, Hannover, Germany, (3) Institute for biostatistics and mathematical modeling, Johann Wolfgang Goethe-University, Frankfurt, Germany, (4) Inserm U748; Université de Strasbourg, Strasbourg, France, (5) Department of Pathology, Stanford University, Stanford, CA, USA and (6) Institut de Biologie et Chimie des Protéines, UMR CNRS 5086, Université Lyon, Lyon, France

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Scientific Programme – Friday, April 9, 2010 TWO INDEPENDENT MECHANISMS IN THE POLYMERASE GENE OF THE HEPATITIS C VIRUS ISOLATE JFH1 CONTRIBUTE TO EFFICIENT REPLICATION IN CELL CULTURE M. Schmitt (1), N. Scrima (2), R. Klein (1), O. Wicht (1), S. Bressanelli (2) and V.Lohmann (1) (1) Department of Infectious Disease, Molecular Virology, University of Heidelberg INF 345, Heidelberg, Germany and (2) Virologie Moléculaire et Structurale, UMR CNRS 2472 Gif-sur-Yvette cedex, France LONG TERM FOLLOW-UP OF INJECTING DRUG USERS SUGGESTS PROTECTIVE IMMUNITY AGAINST HCV REINFECTION AND SUPERINFECTION WITH A SIMILAR GENOTYPE T.J.W. van de Laar (1), M. Rondy (2,3), S.M. Bruisten (1,4), M. Prins (1,4) and M.W. van Ballegooijen (2) (1) Cluster of Infectious Diseases, Public Health Service, Amsterdam, The Netherlands, (2) Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands, (3) European Programme for Intervention Epidemiology Training (EPIET), European Center for Diseases Prevention and Control (ECDC), Stockholm, Sweden, (4) Department of Internal Medicine, Centre for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, Amsterdam, The Netherlands.

10.30-11.00

Coffee Break

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Scientific Programme – Friday, April 9, 2010 11.00-13.00

Plenary session: Global impact of viral persistence

Room A

Chairs:

B. Lina, T. Mertens

Keynote Lectures:

R. Bartenschlager New Insights into Hepatitis C Virus Replication and Persistence N. Almond The development of an AIDS Vaccine: lessons from the past and future challenges for simian models B. Roizman Have herpes simplex viruses hijacked the DNA silencing machinery of host cells to establish latent infections? H. Pfister Papillomaviruses and human cancer: different virus types ask for different prevention strategies

13.00-14.00

Break

14.00-16.30

W12 - Emerging viruses and zoonoses

Chairs:

A. D.M.E. Osterhaus, N. Tordo

Keynote Lectures:

A. D.M.E. Osterhaus Detection and prevention strategies for emerging viruses N. Tordo Dynamics of Puumala virus in bank voles from endemic and non endemic regions for Human Fever with Renal Syndrome (HFRS) in France.

15.00-16.30

Oral presentations

Room A

NOVEL POLYOMAVIRUSES IN WILD AND CAPTIVE NON-HUMAN PRIMATES N. Scuda (1), F. H. Leendertz (2), C. Boesch (3) and B. Ehlers (1) (1) Project group 1 “Novel infectious agents”, Robert Koch-Institut, D-13353 Berlin, Germany; (2) Research Group “Emerging Zoonoses“, Robert Koch-Institut, D-13353 Berlin, Germany and (3) Max Planck Institute for Evolutionary Anthropology, Department of Primatology, Deutscher Platz 6, D-04103 Leipzig, Germany PHYLOGENY OF TICK-BORNE ENCEPHALITIS VIRUSES IN SOUTHERN GERMANY G. Dobler (1), G. Zöller (1), M. Pfeffer (2), F. Hufert (3) and S. Essbauer (1) (1) Bundeswehr Institute of Microbiology, Munich, Germany; (2) Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, Germany and (3) Dept. of Virology, University of Göttingen, Germany SARS-LIKE CORONAVIRUSES IN EUROPEAN HORSESHOE BATS J.F. Drexler (1), F. Gloza-Rausch (2,3), J. Glende (4), V. M. Corman (1), M. Goettsche (2,3), A. Seebens (2), S. Yordanov (5), L. Zhelyazkov (5), U. Hermanns (6), M. A. Müller (1), P. Vallo (7), G. Herrler (4) and C. Drosten (1) (1) Institute of Virology, University of Bonn Medical Centre, Bonn, Germany, (2) Noctalis, Centre for Bat Protection and Information, Bad Segeberg, Germany, (3) Zoological Institute, Department of Ecology, University of Kiel, Kiel, Germany, (4) Institute of Virology, University of Hannover Veterinary Faculty, Hannover, Germany, (5) Forestry Board Directorate of Strandja Natural Park, Malko Tarnovo, Bulgaria, (6) Bat Conservation and Research Group Mecklenburg-Vorpommern, Rostock, Germany and (7) Academy of Sciences of the Czech Republic, Brno, Czech Republic

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Scientific Programme – Friday, April 9, 2010 CROSS-NEUTRALIZATION ACTIVITY AGAINST THE HEMAGGLUTININ OF INFLUENZA A S. Åkerström (1), A. Mirazimi (1) and Y-J Tan (2) (1) Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden and (2) Institute of Molecular and Cell Biology, CAVR, 61 Biopolis Drive, Singapore 138673, Singapore HCMV ENTRY AND REPLICATION INTO PORCINE ENDOTHELIAL CELLS A.L. Millard (1), L. Haeberli (1), M.K. Schneider (2), J.D. Seebach (3), C. Sinzger (4) and N.J. Mueller (1) (1) Division of Infectious Diseases and Hospital Epidemiology, (2) Laboratory for Transplantation Immunology, University Hospital, Zürich, Switzerland, (3) Service of Clinical Immunology and Allergology, University Hospital and Medical Faculty, Geneva, Switzerland and (4) Department of Medical Virology, University of Tübingen, Tübingen, Germany A UNIVERSAL VIRUS DETECTION ASSAY – INNOVATIVE MOLECULAR TOOLS FOR IDENTIFICATION OF NEW VIRUSES C. Uhlenhaut (1), S. McClenahan (1), G. Scherba (2), S. Sosnovtsev (3), K. Bok (3), A.Z. Kapikian (3), K.Y. Green (4) and P.R. Krause (1) (1) Center For Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA, (2) Department of Pathobiology, University of Illinois, Urbana, Illinois, USA, (3) National Institutes Of Health, Bethesda, Maryland, USA and (4) National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA

14.00-16.30

W13 - Adaptive immunity and immune prevention

Room B

Chairs:

G. Karlsson Hedestam, S. Landolfo

Keynote Lectures: 14.00-14.30

G. Karlsson Hedestam Evolution of B cell sub-populations and neutralizing antibodies in response to HIV-1 envelope glycoprotein immunization

14.30-16.30

Oral presentations IMMUNOGENICITY OF DIFFERENT DELIVERY STRATEGIES OF RSV DNA VACCINES IN RHESUS MONKEYS T. Grunwald (1), M. Tenbusch (1), R. Schulte (2), M. Franz (2), D. Hannaman (3), B. Tippler (1), R. Steinman (4), C. Stahl-Hennig (2) and K. Überla (1) (1) Department of Molecular and Medical Virology, Ruhr-Universitaet Bochum, Germany, (2) German Primate Center (DPZ), Leibniz-Institute for Primate Research, Goettingen, Germany, (3) Ichor Medical Systems, Inc., San Diego, CA, USA and (4) Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021, USA INDUCTION OF LONGITUDINAL CROSS-CLADE ANTI-H5N1 IMMUNITY AND PROTECTION BY IMMUNIZATION WITH WHOLE VIRUS H5N1 INFLUENZA CANDIDATE VACCINES N. Sabarth, M.K. Howard, H. Savidis-Dacho, P. Brühl, M. Schwendinger, B.A. Crowe, T.R. Kreil, P.N. Barrett and O. Kistner Biomedical Research Center, Baxter Bioscience, Uferstrasse 15, 2304 Orth/Donau, Austria

43

Scientific Programme – Friday, April 9, 2010 EVALUATION OF IMMUNE RESPONSE TO HIV-1 VIRUS-LIKE PARTICLES EXPRESSING DIFFERENT HIV-1 GLYCOPROTEINS M.L. Visciano (1), M. Tagliamonte (1), M.L. Tornesello (1), L. Lopalco (2), F.M. Buonaguro (1) and L. Buonaguro (1,3) (1) Laboratory of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori “Fondazione G. Pascale”, Naples, Italy, (2) Immunobiology of HIV Unit, Div. of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy and (3) Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, USA CHARACTERIZATION OF A NEW ANTIGENIC DOMAIN ON GLYCOPROTEIN B OF HUMAN CYTOMEGALOVIRUS THAT IS TARGET OF NEUTRALIZING ANTIBODIES N. Tschesnych (1), S. Pötzsch (2), P. Dirauf (3), H. Sticht, T. Winkler (2) and M. Mach (1) (1) Institute for Clinical and Molecular Virology, (2) Nikolaus-Fiebiger-Center for Molecular Medicine and (3) Institute for Biochemistry, University Erlangen-Nuremberg, Germany SKIN-RESIDENT MEMORY T CELLS AND SYSTEMIC PROTECTIVE IMMUNITY ARE EFFICIENTLY INDUCED BY INTRADERMAL TATTOO VACCINATION BUT NOT BY NEEDLE INJECTION G. Gasteiger (1,2), A. Muschaweckh (1), W. Kastenmuller (1,2,3), P.-A.König (1), S. Kisling (1), R. Baier (1), D. H. Busch (4) and I. Drexler (1,2) (1) Institute of Virology and (2) Clinical Cooperation Group “Antigen-specific Immunotherapy, Helmholtz Zentrum München and Technische Universität München; Schneckenburger Str. 8, 81675 München, Germany, (3) Current address: Lymphocyte Biology Section, Laboratory of Immunology, NIAID, NIH and (4) Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität, München; IMMUNE CONTROL OF MURINE CYTOMEGALOVIRUS INFECTION AFTER DELETION OF THE DOMINANT CD8 T CELL EPITOPES R. Holtappels (1), D. Thomas (1), C.O. Simon (1), A. Renzaho (1), S. Ebert (1), C. Krause (1), M. Munks (2), N.K.A Grzimek (1), A. B. Hill (2) and M.J. Reddehase (1) (1) Institut for Virology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany and (2) Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 972392 CHARACTERISTICS OF ANTIBODY RESPONSES IN ENCEPHALITIS VACCINATION BREAKTHROUGHS K. Stiasny, H. Holzmann and F. X. Heinz Institute of Virology, Medical University of Vienna, Vienna, Austria

TICK-BORNE

DESIGN OF RECOMBINANT MVA VECTORS FOR THERAPEUTIC VACCINATION IN A MURINE MODEL OF HBV INFECTION S. Backes, G. Gasteiger, I. Drexler and U. Protzer Institut für Virologie, Helmholtz Zentrum München / Technische Universität München, Munich, Germany

14.00-16.30

W14 - Herpesviruses – oncogenesis and signaling

Chairs:

B. Fleckenstein, T. Schultz

Keynote Lecture: 14.00-14.30

B. Fleckenstein Viral oncoproteins in lymphocyte transformation

44

Room C

Scientific Programme – Friday, April 9, 2010 14.30-16.30

Oral presentations HUMAN HERPESVIRUS 8-ENCODED ORF75 IS A NOVEL ACTIVATOR OF NFKAPPAB M. A. Konrad, O. Milisterfer, R. Jochmann, P. Chudasama and M. Stürzl. Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Schwabachanlage 10, 91054 Erlangen, Germany. A ROLE OF INTERFERON-RESPONSE PATHWAYS IN KAPOSI SARCOMA HERPESVIRUS (KSHV) VIRAL FLICE INHIBITORY PROTEIN-INDUCED ENDOTHELIAL SPINDLE CELL FORMATION K.R. Alkharsah (1), V.V. Singh (1), R. Bosco (3), A. Grundhoff (4), O. Dittrich-Breiholz (2), M. Kracht (2), M. Stürzl (5) and T.F. Schulz (1) (1) Institute of Virology; (2) Institute of Pharmacology, Hannover Medical School, Hannover, Germany; (3) Department of Oncology and Haematology, University of Modena, Modena, Italy, (4) Heinrich Pette Institute, Hamburg, Germany and (5) Department of Surgery, Section of Experimental Surgery, Erlangen, Germany. THE UBIQUITINATING C-TERMINAL HYDROLASE 1 IS INDUCED BY EPSTEIN-BARR VIRUS IN TRANSFORMED B-LYMPHOCYTES AND IS ASSOCIATED WITH MITOTIC CELL SPINDLE A. Bheda, J. Shackelford and J.S. Pagano Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA EPIGENETIC REGULATION OF LAMIN A/C PROMOTER IN CELL LINES CARRYING LATENT EPSTEIN-BARR VIRUS GENOMES F. Bánáti (1), T. Tereh (1), A. Koroknai (1), N. Kovács (1), Zs. Ruzsics (2), F. Lemnitzer (2) and J. Minárovits (1) (1) National Center for Epidemiology, Microbiological Research Group, H-1529, Budapest, Hungary and (2) Max von Pettenkofer-Institute, Ludwig Maximilians-University Munich, Munich 80337, Germany THE HERV K NP9 PROTEIN BINDS TO THE ARG-GLY REPEAT OF EPSTEINBARR VIRUS NUCLEAR ANTIGEN 2 (EBNA2) AND INHIBITS TRANSCRIPTIONAL ACTIVATION H. Gross (1), B. Hu (2), E. Noessner (2), M. Sauter (1), N. Müller-Lantzsch (1) and F.A. Graesser (1) (1) Institut für Medizinische Mikrobiologie Und Hygiene, Abteilung Virologie, Universitätsklinikum Des Saarlandes, 66421 Homburg/Saar, Germany and (2) Institut für Molekulare Immunologie, Helmholtz Zentrum München, 81377 München, Germany FUNCTIONAL COMPLEMENTATION BETWEEN HUMAN AND MOUSE CYTOMEGALOVIRUS G PROTEIN-COUPLED RECEPTOR HOMOLOGUES. N. Davis-Poynter (1), A. Abraham (1), R. Cardin (2) and H.E. Farrell (1) (1) Sir Albert Sakzewski Virus Research Centre & Clinical Medical Virology Centre, Royal Children’s Hospital & University of Queensland, Brisbane, QLD, AUSTRALIA and (2) Division of Infectious Diseases, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, U.S.A INHIBITION OF TOLL-LIKE RECEPTOR (TLR) AND RECEPTOR (IL-1R) SIGNALING PATHWAYS BY CYTOMEGALOVIRUS PROTEIN M45 P. Fliss, C. Mack and W. Brune Division of Viral Infections, Robert Koch-Institute, Berlin, Germany

INTERLEUKIN-1 THE MURINE

TRANSGENIC MODEL FOR KAPOSI SARCOMA-ASSOCIATED HERPESVIRUS (KSHV) LATENCY AND TUMORIGENESIS D.P. Dittmer and S.-H. Sin Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill, USA

45

Scientific Programme – Friday, April 9, 2010 14.00-16.30

W15 - Diagnostics and molecular typing

Chairs:

H. Niesters, E. Puchhammer-Stöckl

Keynote Lecture: 14.00-14.30

H. Niesters Molecular diagnostics in clinical virology. What is next?

14.30-16.30

Oral presentations

Room D

Abbott Award from ESCV: HIV-1 GP41 MUTATIONS 43T AND 50V ELEVATE RESISTANCE LEVELS OF COMMON ENFUVIRTIDE MUTANTS WHILE ALL MUTANTS REMAINED HIGHLY SUSCEPTIBLE TO SIFUVIRTIDE K. Van Laethem (1), K. Covens (1), N. Dekeersmaeker (1), K. Kabeya (2), J. Balzarini (1), S. De Wit (2) and A.-M. Vandamme (1) (1) Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium and (2) AIDS Reference Centre, Division of Infectious Diseases, CHU Saint-Pierre, Brussels Belgium DEVELOPMENT OF RECOMBINANT ELISA FOR DETECTION OF H5 INFLUENZA VIRUS ANTIBODIES A. Shehata (1), P. Fiebig (1), H. Sultan (2) and U. G. Liebert (1) (1) Institute of Virology, Leipzig University, Leipzig, Germany and (2) Avian Diseases Department, Faculty of Veterinary Medicine, Minoufiya University, Egypt MOLECULAR AND CONVENTIONAL DETECTION AND TYPING OF ENTEROVIRUS AND HUMAN PARECHOVIRUS GENOTYPES FROM STOOL SAMPLES K. S.M. Benschop, R. P. Minnaar, G. Koen, H. W. M. van Eijk, K. Dijkman, B. Westerhuis, R. Molenkamp and K. C. Wolthers Dept. of Clinical Virology, Dept. of Medical Microbiology, Academic Medical Center, Amsterdam BROAD-RANGE SURVEY OF TICK-BORNE BACTERIAL, PROTOZOAN AND VIRAL PATHOGENS M. W. Eshoo (1), O. Nolte (2), C. D. Crowder (1), H. E. Matthews (1), Feng Li (1), R. Sampath (1) and D. J. Ecker (1) (1) Ibis Biosciences, Inc., Carlsbad, CA 92008 USA and (2) Laboratory Brunner, Konstanz Germany SERODIAGNOSIS (IGG, IGM, IGG-AVIDITY) OF HUMAN PARVOVIRUS 4: OCCURRENCES IN POPULATIONS AND IDENTIFICATION OF PRIMARY INFECTIONS K. Hedman (1,2), A. Lahtinen (1), L. Hedman (1,2), A. Kumar (1), K. Liitsola, P. Kivela (4), M. Ristola (4), M. Lappalainen (2) and M. Saderlund-Venermo (1) (1) Departments Of Virology, Haartman Institute, University Of Helsinki, (2) Helsinki University Central Hospital Laboratory Division, (3) Epidemiologic Surveillance And Response Unit, National Institute For Health And Welfare and (4) Division Of Infectious Diseases, Helsinki University Central Hospital, Helsinki, Finland IDENTIFICATION OF NOVEL, SEROLOGICALLY REACTIVE RECOMBINANT VARICELLA-ZOSTER VIRUS (VZV) ANTIGENS BY A SYSTEMATIC, GENOMESCALE APPROACH M.G. Vizoso Pinto (1), K.-I. Pfrepper (2), C. Noelting (2), T. Janke (2), A. Lueking (3), J. Haas1 (4), H. Nitschko (1), G. Jaeger (1) and A. Baiker (1). (1) Max von Pettenkofer-Institute, Virology, Munich, Germany; (2) Mikrogen GmbH, Neuried, Germany; (3) Protagen AG, Dortmund, Germany and (4) University of Edinburgh, Division of Pathway Medicine, Edinburgh, UK

46

Scientific Programme – Friday, April 9, 2010 ANALYSIS OF HUMAN CYTOMEGALOVIRUS STRAIN DIVERSITY IN LUNG TRANSPLANT RECIPIENTS BY USING ULTRADEEP PYROSEQUENCING I. Görzer (1), C. Gülly (2), S. Trajanoski (2) and E. Puchhammer-Stöckl (1) (1) Institute of Virology, Medical University of Vienna and (2) Center for Medical Research, Medical University of Graz, Austria SEROPREVALENCE OF THE NOVEL HUMAN PARVOVIRUS, PARV4, IN INDIVIDUALS WITH AND WITHOUT A HISTORY OF PARENTERAL EXPOSURE C.P. Sharp (1), A. Lail (2), S. Donfield (2), R. Simmons (3), C. Leen (4), P. Klenerman (3), E. Delwart (5), E.D. Gomperts (6) and P. Simmonds (1) (1) Centre for Infectious Diseases, University of Edinburgh, Edinburgh, UK, (2) Department of Biostatistics, Rho, Inc., Chapel Hill, USA, (3) Peter Medawar Centre for Pathogen Research, University of Oxford, Oxford, UK, (4) Regional Infectious Diseases Unit, Western General Hospital, Edinburgh, UK, (5) Blood Systems Research Institute and Department of Laboratory Medicine, University of California - San Francisco, San Francisco, USA and (6) Department of Haematology, Childrens' Hospital Los Angeles, Los Angeles, USA

16.30-17.00

Coffee Break

47

Scientific Programme – Saturday, April 10, 2010

Saturday, April 10, 2010 08.30-10.30

W16 - Retroviruses

Room A

Chairs:

C. R. M. Bangham, M. Pistello

Keynote Lecture: 08.30-09.00

C. R. M. Bangham How does HTLV-1 persist in vivo?

09.00-10.30

Oral presentations SPECIFIC UPREGULATION OF THE TUMOUR MARKER PROTEIN FASCIN BY THE ONCOPROTEIN TAX OF HUMAN T CELL LYMPHOTROPIC VIRUS TYPE 1 (HTLV-1) A.K. Kress (1), R. Grassmann (1,2) and B. Fleckenstein (1) (1) Institute of Clinical and Molecular Virology, University of Erlangen-Nuremberg, Erlangen, Germany, (2) deceased. RNA EXPORT CONTROL IN MURINE LEUKEMIA VIRUS. V. Melhorn (1), D. Zychlinski (1,2), V. Cordes (3) and J. Bohne (1) (1) Cell and Virus Genetics, Institute for Virology, Hannover Medical School, Germany, (2) Experimental Hematology, Hannover Medical School, Germany, and MPI for Biophysical Chemistry, Göttingen, Germany ACTIVATION OF NF-KB PATHWAY IS INDUCED BY INTERACTION OF HTLV2 TAX PROTEIN WITH RELA AND TAB2 FACTORS F. Avesani, M.G.Romanelli, M. Turci, G. Di Gennaro, C. Bidoia and U. Bertazzoni. Department of Mother and Child, Biology and Genetics, University of Verona, Strada Le Grazie 8, 37134 Verona, Italy. DIFFERENTIAL REGULATION OF HUMAN ENDOGENOUS RETROVIRUSES OF THE W FAMILY BY HIV-1: IMPLICATIONS FOR NEURO-AIDS. A. Mei (1), G. Mameli (1), C. Serra (1), L. Poddighe (1), E. Uleri (1), B.E. Sawaya (2), K. Khalili (3) and A. Dolei (1) (1) Section of Microbiology, Department of Biomedical Sciences and Center of Excellence for Biotechnology Development and Biodiversity Research, (2) Department of Neurology and (3) Department of Neuroscience, Temple University, Philadelphia, PA 19140. IMPACT AND ANTAGONISM OF RODENT CD317-IMPOSED INHIBITION OF RETROVIRAL RELEASE C. Goffinet, S. Schmidt, C. Kern, L. Oberbremer and O.T. Keppler Department Of Infectious Diseases, Virology, University Of Heidelberg, Heidelberg, Germany. RATIONAL DESIGN OF AN HIV-INHIBITORY PROTEIN TARGETING THE CELLULAR COFACTOR SAM68 B. Asbach (1), C. Ludwig (1), K. Saksela (2) and R. Wagner (1) (1) Institute of Medical Microbiology & Hygiene, Molecular Microbiology & Gene Therapy, University of Regensburg, Germany and (2) Department of Virology, Haartman Institute, University of Helsinki, Finland

08.30-10.30

W17 - Systems biology of viral replication

Chairs:

P. Ghazal, K. Saksela

Keynote Lecture: 08.30-09.00

P. Ghazal Systems analysis of innate immune mechanisms in infected macrophages

48

Room B

Scientific Programme – Saturday, April 10, 2010 09.00-10.30

Oral presentations HIGH-THROUGHPUT SCREENING OF TROY: IN SEARCH OF THE ACHILLES' HEEL OF POXVIRUS INFECTION J. Mercer and A. Helenius ETH Zurich, Institute of Biochemistry, Zurich, Switzerland APPLYING SYSTEMS BIOLOGY TO SEVERE ACUTE RESPIRATORY CORONAVIRUS AND ITS HUMAN HOST: IDENTIFICATION OF A CELLULAR TARGET AND ITS INHIBITOR FOR ANTIVIRAL INTERVENTION J. Schöpf (1), S. Pfefferle (2), M. Kögl (3), P. Herzog (2), M.A Müller (2), D. Muth (2) T. Kuri (4), F. Ebel (1), C. Friedel (5), R. Zimmer (5), F. Weber (4), J. Haas (1), H.-J. Thiel (6), G. Herrler (7), V. Thiel (8), C. Drosten (2), A. von Brunn (1) and S. Kallies (2) (1) Max-von-Pettenkofer Institut, LMU München (D), (2) IfV Rheinische Friedrich-WilhelmsUniv. Bonn (D), BNI Hamburg, (3) DKFZ, Heidelberg (D), (4) IMMH, Albert-LudwigsUniversität Freiburg (D), (5) Inst. Für Informatik, LMU München (D), (6) Veterinärmedizin Justus-Liebig Universität Gießen (D), (7) Tierärztliche Hochschule Hannover (D) and (8) Kantonsspital St. Gallen, (CH) HOST GENETICS CONTRIBUTE TO VIRAL miRNA FUNCTION IN VIVO L. Dölken (1), A. Krmpotic (2), S. Kothe (1), L. Marcinowski (1), M. Tanguy (3), J. Perot (3), Z. Ruzsics (1), F. Erhard (4), C.C. Friedel (4), R. Zimmer (4), S. Pfeffer (3), S. Jonjic (2) and U.H. Koszinowski (1) (1) Max von Pettenkofer-Institute, Ludwig-Maximilians-Universität München, Germany, (2) Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, Croatia, (3) Institut de biologie moléculaire et cellulaire du CNRS, Université de Strasbourg, France and (4) Institute for Informatics, Ludwig-Maximilians-Universität München, Germany IMPACT OF mTOR ON ADENOVIRUS INFECTION N. Wolfrum (1), N. Imelli (2), B. Rusconi (2), K. Boucke (2) and U.F. Greber (1) (1) University of Zurich, Zurich, Switzerland and (2) Swiss Federal Institute of Technology, Zurich, Switzerland RNAi SCREEN TO IDENTIFY HUMAN HOST CELL FACTORS REQUIRED FOR HIV-1 RELEASE J. Hermle (1), K. Börner (1), J. Mazur (2), N.P. Brown (3,4), M. Anders (1), B. Müller (1), R. Schneider (3), H. Erfle (5), R. Pepperkok (6), L. Kaderali (2), M.J. Lehmann (1) and H.-G. Kräusslich (1) (1) Virology, Department of Infectious Diseases, University of Heidelberg, Heidelberg, Germany, (2) Viroquant Research Group Modelling, BIOQUANT, University of Heidelberg, Heidelberg, Germany, (3) Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, (4) Data integration and knowledge management, BIOQUANT, University of Heidelberg, Heidelberg, Germany, (5) ViroquantCellNetworks RNAi Screening Facility, BIOQUANT, University of Heidelberg, Heidelberg, Germany and (6) Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany A SYSTEMS IMMUNOLOGY APPROACH TO MODEL PLASMACYTOID DENDRITIC CELL FUNCTION IN CYTOPATHIC VIRUS INFECTIONS G. Bocharov (1), R. Züst (2), L. Cervantes-Barragan (2,3), T. Luzyanina (4), V. Thiel (2), and B. Ludewig (2) (1) Institute of Numerical Mathematics, Russian Academy of Sciences, Moscow, Russia, (2) Research Department, Kantonal Hospital St. Gallen, St. Gallen, Switzerland, (3) Unidad de Investigatión Médica en Immunoquimica, Hospital de Especialidades México City, México and (4) Institute of Mathematical Problem in Biology, Russian Academy of Sciences, Pushzhino, Russia.

49

Scientific Programme – Saturday, April 10, 2010 08.30-10.30

W18 - Oncolytic viruses and gene therapy

Chairs:

R. Cattaneo, R. Manservigi

Keynote Lecture: 08.30-09.00

R. Cattaneo Viruses as cancer therapeutics: Three attack points

09.00-10.30

Oral presentations

Room C

ZINC-FINGER NUCLEASE (ZFN) INDUCED GENE TARGETING BY AAV VECTORS K.Gellhaus (1), E.M. Handel (2), F. Muller-Lerch (1), R. Heilbronn (1) and T. Cathomen (1,2) (1) Institute Of Virology (CBF), Charité Medical School, Berlin, Germany and (2) Department Of Experimental Hematology, Hannover Medical School, Hannover, Germany DEVELOPMENT AND CHARACTERIZATION OF TARGETED LENTIVIRAL VECTORS PSEUDOTYPED WITH THE TUPAIA PARAMYXOVIRUS GLYCOPROTEINS T. Enkirch (1), S. Funke (2), B. Hoyler (1), W. Stremmel (1), C.J. Buchholz (2) and C. Springfeld (1) (1) Universitätsklinikum Heidelberg, Abteilung Innere Medizin IV, Heidelberg, Germany and (2) Paul-Ehrlich-Institut, Division of Medical Biotechnology, Langen, Germany IN VIVO EFFICACY AGAINST MAMMARY TUMORS OF A RECOMBINANT HERPES SIMPLEX VIRUS (HSV) FULLY RETARGETED TO HER-2 L. Menotti (1), V. Gatta (1), G. Nicoletti (2), S. Croci (3), L. Landuzzi (2), C. De Giovanni (3), P. Nanni (3), P.L. Lollini (4) and G. Campadelli-Fiume (1) (1) Department of Experimental Pathology, Section on Microbiology and Virology, University of Bologna, Bologna, Italy, (2) Laboratory of Oncologic Research, Rizzoli Orthopaedic Institute, Bologna, Italy, (3) Department of Experimental Pathology, Section of Cancer Research and (4) Department of Hematology and Oncological Sciences, University of Bologna, Bologna, Italy POTENTIATING CANCER IMMUNOTHERAPY USING AN ONCOLYTIC VIRUS B.W. Bridle (1), K.B. Stephenson (1), J.E. Boudreau (1), S. Koshy (1), N. Kazdhan (1), E. Pullenayegum (2), J. Brunellière (1), J.L. Bramson (1), Y. Wan (1) and B.D. Lichty (1) (1) Centre for Gene Therapeutics, Department of Pathology and Molecular Medicine and (2) Centre for Evaluation of Medicines, Department Clinical Epidemiology & Biostatistics, McMaster University, Hamilton, ON, Canada, L8N 3Z5 AN ADENOVIRAL VECTOR FOR SOMATIC INTEGRATION ENABLES PERSISTENT PHENOTYPIC CORRECTION IN MICE AND A CANINE MODEL FOR HEMOPHILIA B M. Hausl (1), W Zhang (1), N. Müther (1), C. Rauschhuber (1), H.G. Franck (2), E.P. Merricks (2), T.C. Nichols (2), M.A. Kay (3) and A. Ehrhardt (1) (1) Max von Pettenkofer-Institut, Department of Virology, Ludwig-Maximilians-Universität Munich, Pettenkofer Str. 9A, 80336 Munich, Germany, (2) Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA and (3) Departments of Pediatrics and Genetics, School of Medicine, Stanford University, 269 Campus Drive Rm 2105, Stanford, California, 94305, USA MEMBRANE-ANCHORED AND SECRETED C PEPTIDES AS HIV ENTRY INHIBITORS J. Kimpel, L. Egerer, J. Kahle, F. Brauer, A. Volk, M. Hartmann, F.G. Hermann, S. Newrzela, and D. von Laer Applied Virology and Gene Therapy, Georg-Speyer-Haus, Frankfurt am Main, Germany

50

Scientific Programme – Saturday, April 10, 2010 08.30-10.30

W19 - Plant Viruses

Room D

Chairs:

R. Flores, G. P. Martelli

Keynote Lectures:

R. Flores Viroid-host interactions: recent advances in replication, movement and pathogenesis M. Taliansky The cell nucleus and plant virus systemic infections

09.30-10.30

Oral presentations FUNCTIONAL STUDIES OF THE BIPARTITE BEGOMOVIRUS CP PROMOTER G. Sunter (1), Janet L. Sunter (1) and G. Lacatus (2) (1) Department of Biology, University of Texas at San Antonio, San Antonio TX 78249 USA and (2) Tumor Virology Program, Greheey Children's Cancer Research Institute, The University of Texas Health Sciences Center, San Antonio, San Antonio, TX 78229 USA. STUDIES ON THE REPLICATION OF TOMBUSVIRUS SATELLITE RNAs L. Rubino and M. Russo Istituto di Virologia Vegetale, CNR, Bari, Italy RNA-CONTROLLED SELF-ASSEMBLY OF TOBACCO MOSAIC VIRUSDERIVED TEMPLATE ARCHITECTURES: A NOVEL SCAFFOLDING TOOLKIT FOR FUNCTIONAL NANOCOMPONENTS C. Wege (1), A. Mueller (1), F. Geiger (2), F. Eber (1), S. Eiben (1), A. Kadri (1), J. Spatz (2), C. Krill (3), A. Bittner (4), H. Gliemann (5) and H. Jeske (1). (1) University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany, (2) Max-Planck-Institute of Metals Research, Dpt. New Materials and Biosystems, Stuttgart, Germany, (3) University of Ulm, Institute of Micro- and Nanomaterials, Ulm, Germany, (4) CIC NanoGUNE, Donostia-San Sebastian, Spain and (5) Institute for Technical Chemistry/Functional Interfaces (ITC/IFG), Forschungszentrum Karlsruhe (FZK), Eggenstein-Leopoldshafen, Germany. SMALL RNAS AND SILENCING SUPPRESSORS: TWO KEY CHALLENGERS IN POTYVIRAL INFECTIONS A. Valli (1), A. Carbonell (1), G. Dujovny (1), J.C. Oliveros (1), A. Molnar (2), D. Baulcombe (2) and J.A. García (1) (1) Department of Plant Molecular Genetics, National Centre of Biotechnology-CSIC, 28049 Madrid, Spain and (2) Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom.

10.30-11.00

Coffee Break

51

Scientific Programme – Saturday, April 10, 2010 11.00-13.00

Plenary session: Novel frontiers in virology

Chairs:

C. De Giuli Morghen, G. Palù

11.00-12.00 Keynote Lectures:

Room A

Y. Chang Discovery of a New Human Tumor Virus, Merkel Cell Polyomavirus (MCV) L. Naldini Targeting gene transfer by microRNA regulation and site-specific integration

12.00-12.30

Gesellschaft für Virologie - Loeffler Frosch medal Awardee: P. Vogt From Rous sarcoma to cancer genomics: the emergence of therapeutic targets

12.30-13.00

Italian Society for Virology - Pioneer in Virology Lecture U. Koszinowski Pygmalion dreams while moulding CMV

13.00-14.00

Break

14.00-16.30

W20 – Alpha- and Beta- herpesviruses. Replication and genetics

Chair:

T. C. Mettenleiter, W. Brune

Keynote Lecture: 14.00-14.30

T. C. Mettenleiter Assembly and Exit Pathway of Herpesviruses

14.30-16.30

Oral presentations

Room A

THE HUMAN CYTOMEGALOVIRUS PROTEIN UL 78 IS MAINLY LOCATED IN THE ENDOPLASMATIC RETICULUM AND IS ABLE TO INTERACT WITH DIFFERENT VIRAL PROTEINS S. Wagner, A. Schreiber, F. Arnold, M. Willmann, A. Schubert, R. Ansorge, T. Mertens and D. Michel Institute of Virology , University Hospital Ulm, 89081Ulm, Germany CONSTRUCTION OF BACTERIAL ARTIFICIAL CHROMOSOME OF DUCK ENTERITIS VIRUS STRAIN 2085 J.C. Wang (1), A. Reum (1), S.J. Roth (1) and N. Osterrieder 1* 1 Institut für Virologie, Freie Universität Berlin, Berlin, Germany and *Corresponding author. Mailing address: Institut für Virologie, Freie Universität Berlin, Berlin. Philippstrasse 13, Haus 18, 10115 Berlin, Germany HUMAN RAB 6 IS A MAJOR DETERMINANT OF HERPES SIMPLEX VIRUS TYPE 1 (HSV-1) MATURATION C. Gonzalez-Lopez, M. Hollinshead, C. Sayers and G. Elliott Department of Virology, Faculty of Medicine, Imperial College London, London, W2 1PG, United Kingdom THE HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K IS REQUIRED FOR EFFICIENT HERPES SIMPLEX VIRUS-1 REPLICATION H. Striebinger (1), T. Schmidt (2), J. Haas (1) and S.M. Bailer (1). (1) Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität München, München, Germany and (2) Department Virologie, Universität des Saarlandes, Homburg/Saar, Germany

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Scientific Programme – Saturday, April 10, 2010 THE INTERACTION OF THE VIRAL REGULATORY PROTEIN PUL69 WITH THE CELLULAR mRNA EXPORT FACTOR UAP56 IS CRITICAL FOR EFFICIENT REPLICATION OF HUMAN CYTOMEGALOVIRUS R. Müller, A. Giede-Jeppe, M. Thomas and T. Stamminger Institue For Clinical And Molecular Virology, University Hospital Erlangen, University of Erlangen-Nuremberg, Erlangen, Germany ACTIVATION OF SILENT TRANSGENES BY VIRUS INFECTION VIA ORILYT DRIVEN DNA AMPLIFICATION H. Muehlbach (1), C. A Mohr (1), Z. Ruszics (1), M. Schneider (2), E. Wolf (2) and U.H. Koszinowski (1) (1) Max-von-Pettenkofer Institut, Department of Virology and (2) Department of Molecular Breeding and Biotechnology, Ludwigs-Maximilian University, Munich, Germany HUMAN CYTOMEGALOVIRUS IMMUNOEVASION PROTEINS GPUS2 AND GPUS11 COOPERATE WITH GPUS3 TO IMPAIR MHC CLASS I SURFACE EXPRESSION AND ANTIGEN PRESENTATION BY INFECTED CELLS J. Hesse, K. Besold and B. Plachter. Institute of Virology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz HIGHLY SENSITIVE DETECTION OF MCMV REACTIVATION WITH A POLYFUNCTIONAL REPORTER MUTANT A. Marquardt (1), S. Halle (2), T. Z Veres.(3), A. Braun (3), R. Förster (2), M. Messerle (1) and A. Busche (1) (1) Institute of Virology, and (2) Institute of Immunology, Hannover Medical School and (3) Department of Immunology, Allergology and Immunotoxicology, Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany

14.00-16.30

W21 - RNA Viruses

Room B

Chairs:

K. K. Conzelmann, F. M. Ruggeri

Keynote Lecture: 14.00-14.30

K. K. Conzelmann Genetics and host cell interplay of Mononegavirales: Conquering worlds with a handful of genes

14.30-16.30

Oral presentations NYAMANINIVIRUS, A NEWLY IDENTIFIED MEMBER OF MONONEGAVIRALES HAS AN ATYPICAL GENOME ORGANIZATION M. Schuetze, and U. Schneider. Department of Virology, University of Freiburg, Freiburg, Germany

THE

DISSECTION OF NONSTRUCTURAL PROTEIN BIOSYNTHESIS OF CLASSICAL SWINE FEVER VIRUS (CSFV) B. Lamp, G. Roman-Sosa, H.-J. Thiel and T. Rümenapf Institut für Virologie (FB Veterinärmedizin), Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, 35392 Giessen, Germany DOMAIN STRUCTURE OF LASSA VIRUS L PROTEIN L. Brunotte, K. Kleinsteuber, M. Hass, M. Lelke, B. Becker-Ziaja and S. Günther Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany ROLE OF CORONAVIRUS NUCLEOCAPSID PROTEIN ON RNA SYNTHESIS S. Zuñiga, J. L. G. Cruz, I. Sola, M. Becares and L. Enjuanes Department of Molecular and Cell Biology. Centro Nacional de Biotecnologia (CNB), CSIC. Darwin, 3. Campus Universidad Autónoma, Cantoblanco. Madrid 28049. Spain

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Scientific Programme – Saturday, April 10, 2010 FUNCTIONS AND INTERACTIONS OF CORONAVIRUS REPLICASE GENEENCODED PROTEINS T. Hertzig, N. Karl and J. Ziebuhr Centre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, The Queen’s University of Belfast, Belfast BT9 7BL, United Kingdom DYNAMICS OF CORONAVIRUS REPLICATION COMPLEXES M.C. Hagemeijer (1), M.H. Verheije (1), M. Ulasli (2), F. Reggiori (2), P.J.M. Rottier (1) and C.A.M. de Haan (1) (1) Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands and (2) Department of Cell Biology. University Medical Centre Utrecht, Utrecht, The Netherlands BIOCHEMICAL CHARACTERIZATION OF THE ISOLATED NIDOVIRUS REPLICATION AND TRANSCRIPTION COMPLEX. A.H. de Wilde, P.T. M. van Boh eemen, E. J. Snijder and M. J. van Hemert Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands IFN-β MODULATION OF THE HOST AUTOPHAGY AS A NOVEL ANTIVIRAL STRATEGY H. Khalil (1), M. A. Al-Zeer (1), A. Karlas (1), H. M. Al-Younes (2) and T. F. Meyer (1) (1) Max Planck Institute for Infection Biology, Department of Molecular Biology, Berlin, Germany and (2) Department of Biological Sciences, University of Jordan, Amman, Jordan

14.00-16.30

W22 - Diagnostics and antivirals

Chairs:

J. Balzarini, M.R. Capobianchi

14.30-16.30

Oral presentations

Room C

HEPATITIS C VIRUS NS3/4A PROTEASE AND NS5B POLYMERASE POLYMORPHISM IN WORLDWIDE ISOLATES: ANTICIPATED PROBLEMS FOR STAT-C THERAPIES IN NON-1 GENOTYPES V. Sentandreu (1,3), F. González-Candelas (1,2,3), A. Moya (1,2,3) and F. Xavier LópezLabrador (1,2,3) (1) Evolutionary Genetics Unit, Institut Cavanilles, University of Valencia. Apt. Of. 22085, 46071 Valencia, Spain, (2) Center for Public Health Research (CSISP), Public Health Dept., Generalitat Valenciana, 46010 Valencia, Spain and (3) CIBER-ESP (Centro de Investigación Biomédica en Epidemiología y Salud Publica, ISCIII, Spain. DEVELOPMENT OF REPLICATION COMPETENT CHIMAERAS OF HCV WITH EXCHANGED NS3 AND NS4A GENES FROM HCV GENOTYPES 1-6 FOR DIRECT PHENOTYPIC ASSESSMENT OF RESISTANCE TO PROTEASE INHIBITORS AMONG TREATED PATIENTS I. Imhof, and P. Simmonds Centre for Infectious Diseases, University of Edinburgh, Summerhall, Edinburgh, UK LINKING HUMAN CYTOMEGALOVIRUS DRUG RESISTANCE MUTATIONS TO RESISTANCE PHENOTYPES M. Chevillotte (1), I. Ersing (1), B.M. Meier (3), F.M. Lin (4), H.A. Kestler (2,3), T. Mertens (1) and J. Von Einem (1) (1) Institute of Virology, University Hospital Ulm, D-89081 Ulm, Germany, (2) Internal Medicine I, University Hospital Ulm, D-89081 Ulm, Germany, (3) Institute of Neural Information Processing, University of Ulm, D-89069 Ulm, Germany and (4) Institute Of Bioinformatics and Systems Biology, National Chiao Tung University, Hsin-Chu 300, Taiwan, Republic of China

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Scientific Programme – Saturday, April 10, 2010 GENERATION AND CHARACTERISATION OF A HUMANIZED MONOCLONAL ANTIBODY FOR PROPHYLAXIS AND THERAPY OF HERPES SIMPLEX VIRUS INFECTIONS A.Krawczyk (1), J. Krauss (2), M.A.E. Arndt (2), A.M. Eis-Huebinger (3), K.E. Schneweis (3), C.R. Martin (4), L. Grosse-Hovest (5) and M. Roggendorf (1) (1) Universitätsklinikum Essen, (2) Nationales Centrum für Tumorerkrankungen Heidelberg, (3) Universitätsklinikum Bonn, (4) Univ. College London and (5) Un. Tübingen, Germany ANTIVIRAL EFFECTS OF RESVERATROL ON EPSTEIN BARR VIRUS LATENT AND LYTIC INFECTION A. De Leo (1), G. Arena (1), C. Stecca (1), E. Lacanna (1), M. Raciti (2). G. Oliviero (1) and E. Mattia (1) (1) Dept. of Public Health Sciences and (2) Dept. of Experimental Medicine, Univ. of Rome "Sapienza", Italy EVALUATION OF THE CLINICAL SIGNIFICANCE OF HUMAN PAPILLOMAVIRUS GENOTYPE 53 E. Padalko (1), L. Van Renterghem (1), M. Bamelis (2), A. De Mey (2), Y. Sturtewagen (2), H. Vastenavond (2), E. Verbraekel (1), M. Benczik (3), T. Takacs (3), C. Jeney (3), B. Bengyel (3), S. Weyers (4) and M. Praet (2) (1) Departement of Clinical Chemistry, Microbiology and Immunology, (2) Pathology and (4) Gynecology, Ghent University Hospital, Ghent, Belgium and (3) GenoID Molecular Diagnostics Laboratory, Budapest, Hungary TEN YEARS OF EXPERIENCE AND VALIDATION OF A LOW COST HUMAN PAPILLOMAVIRUS (HPV) GENOTYPING ASSAY C. Estrade (1), P.A. Menoud (2), D. Nardelli-Haefliger (3) and R. Sahli (1) (1) Institute of Microbiology, CHUV, University of Lausanne, (2) Laboratoire Unilabs, Lausanne, (3) Department of Urology and Institute of Microbiology, CHUV, University of Lausane, Switzerland, and (1; 3) WHO Regional Reference HPV Laboratory For Europe PERFORMANCE OF DIRECT FLUORESCENT ANTIBODY STAINING, POINTOF-CARE RAPID ANTIGEN TEST, AND VIRUS ISOLATION FOR THE DETECTION OF NOVEL SWINE ORIGIN INFLUENZA A (H1N1) VIRUSES IN COMPARISON TO RT-PCR IN RESPIRATORY SPECIMENS T. Ganzenmueller, C. Henke-Gendo, B. Hilfrich, T.F. Schulz and Albert Heim Institute of Virology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany EXTERNAL QUALITY CONTROL FOR GENOME AND ANTIGEN DETECTION OF NEW INFLUENZA A(H1N1), AVIAN INFLUENZA A(H5N1) AND SEASONAL INFLUENZA H. Zeichhardt (1,2), V. Lindig (1,3), B. Schweiger (4) and H.P. Grunert (1,2,3) (1) Charité-Universitaetsmedizin Berlin, Campus Benjamin Franklin, Institut fuer Virologie, Berlin, (2) INSTAND, Gesellschaft zur Foerderung der Qualitaetssicherung in medizinischen Laboratorien e.V., Duesseldorf, (3) Institut für Biotechnologische Diagnostik, Berlin and (4) Robert Koch-Institut, Nationales Referenzzentrum für Influenza, Berlin, Germany CALIBRATED MEASUREMENT OF HBsAg CONCENTRATION IN NG/ML FOR HEPATITIS B VIRUS GENOTYPES A-H AND COMPARISON WITH HBV DNA LEVELS W.H. Gerlich (1), U. Wend (1), C.G. Schüttler (1), M. Nübling (2) and M. Chudy (2) (1) Institute Of Medical Virology, Justus Liebig University, Giessen, Germany and (2) Paul Ehrlich-Institute, Langen, Germany

14.00-16.30

W23 - Virus structure, assembly and exit

Chairs:

C. Risco, C. Parolin

Keynote Lecture: 14.00-14.30

C. Risco 3D EM and macromolecular mapping unveil the unique architecture of virus factories

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Room D

Scientific Programme – Saturday, April 10, 2010 14.30-16.30

Oral presentations STRUCTURE OF ADENOVIRUS TYPE 21 KNOB IN COMPLEX WITH CD46 REVEALS KEY DIFFERENCES IN RECEPTOR CONTACTS AMONG SPECIES B ADENOVIRUSES K. Cupelli (1), S. Müller (1), D. Persson (1), M. Jost (1), N. Arnberg (2) and T. Stehle (1,3) (1) Interfaculty Institute for Biochemistry, University of Tübingen, Tübingen, Germany, (2) Division of Virology, Department of Clinical Microbiology, Laboratory for Molecular Infection Medicine in Sweden, Umeå University, SE-901 85 Umeå, Sweden and (3) Department of Pediatrics, Vanderbilt University School of Medicine,Nashville, TN 37232, USA STRUCTURAL STUDIES ON ADENOVIRUS MATURATION A. J. Pérez-Berná (1), R. Marabini (2), S. H. W. Scheres (1), R. Menéndez-Conejero (1), I. P. Dmitriev (3), D. T. Curiel (3), W. F. Mangel (4), S. J. Flint (5) and C. San Martín (1) (1) Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Madrid, (2) Escuela Politécnica Superior, Universidad Autónoma de Madrid, Madrid, España, (3) The Gene Therapy Center, University of Alabama at Birmingham, Birmingham, USA, (4) Biology Department, Brookhaven National Laboratory, Upton, NY, USA and (5) Department of Molecular Biology, Princeton University, Princeton, NJ, USA DISSECTION OF THE MOLECULAR MECHANISMS LINKING HCMV TO THE MVB PATHWAY M. Caduco (1), C. Parolin (2), J. von-Einem (3), G. Campadelli-Fiume (4), G. Palù (1) and A. Calistri (1) (1) Department of Histology, Microbiology and Medical Biotechnologies, Section of Microbiology and Virology, University of Padua, Padua, Italy, (2) Department of Biology, University of Padua, Padua, Italy, (3) Uniklinik Ulm, University of Ulm, Ulm, Germany and (4) Department of Experimental Pathology, Section of Microbiology and Virology, University of Bologna, Bologna, Italy. THE TEGUMENT PROTEIN UL71 OF HUMAN CYTOMEGALOVIRUS IS IMPORTANT FOR LATE ENVELOPMENT PROCESSES AND AFFECTS MULTIVESICULAR BODIES. D. Fischer (1), M. Schauflinger (1,2), S. Landwehr (1,2), P. Walther (2), T. Mertens (1) and J. von Einem (1) (1) Institute of Virology, University Hospital Ulm and (2) Electron Microscopy Facility, University Ulm, 89081 Ulm, Germany CYTOMEGALOVIRAL NUCLEAR CAPSID EGRESS THROUGH LAMINADEPLETED AREAS INDUCED BY VIRAL AND CELLULAR PROTEIN KINASE ACTIVITY J. Milbradt (1), R. Webel (1), S. Auerochs (1), H. Sticht (2) and M. Marschall (1) (1) Institute for Clinical and Molecular Virology, and (2) Division of Bioinformatics, Institute of Biochemistry, University of Erlangen-Nuremberg, Erlangen, Germany IDENTIFICATION OF NOVEL E1E2 DOMAINS IMPORTANT FOR HEPATITIS C VIRUS CELL ENTRY PROCESS USING CHIMERIC HETERODIMERS FROM DIFFERENT GENOTYPES G. Maurin, J. Fresquet, O. Granio, F-L Cosset and D. Lavillette. Université de Lyon, UCB-Lyon1, IFR128, Lyon, France ; INSERM, U758, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France. ON THE COMPLEXITY OF GIANT VIRUSES – MIMIVIRUS AS A CASE STUDY Y. Mutsafi, N. Zauberman and A. Minsky Departmant of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel

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Scientific Programme – Saturday, April 10, 2010 MICROSCOPICAL ANALYSIS OF HIV-1 ASSEMBLY IN MACROPHAGES H. Koppensteiner (1), R. Gaudin (2), C. Banning (1), B. Holstermann (1), C. Schneider (1), H. Hohenberg (1), P. Benaroch (2) and M. Schindler (1) (1) Heinrich-Pette-Institute for Experimental Virology and Immunology at the University of Hamburg, Germany and (2) Institut Curie, Inserm U932 Intracellular transport and Immunity, Paris, France 16.30-17.00

Coffee Break

57

Scientific Programme – Sunday, April 11, 2010

Sunday, April 11, 2010 08.30-10.00

W24 - Viral persistence and immune evasion

Chairs:

N. Mueller Lantzsch, H. Wolf

08.30-09.15

Gesellschaft für Virologie - Loeffler-Frosch Lecture and Prize

Keynote Lecture:

D. Pinschewer Arenaviruses: immunity, immunopathology and vaccines

09.15-10:00

Oral presentations

Room A

REGULATORY T-CELL DEPENDENT SUPPRESSION OF CD4+ AND CD8+ T CELL RESPONSES DURING ACUTE RETROVIRAL INFECTION S. R. Nair (1). G. Zelinskyy (1), K. K. Dietze (1), S. Schimmer (1), G. Kassiotis (2), T. Sparwasser (3) and U. Dittmer (1) (1) Institute for Virology of the University Clinics in Essen, University of Duisburg-Essen, Germany, (2) Division of Immunoregulation, MRC National Institute for Medical Research, The Ridgeway, London, United Kingdom and (3) TWINCORE, Zentrum für Experimentelle und Klinische Infektionsforschung, Hannover, Germany. PRIMING OF CD8 T CELLS AGAINST MCMV-ENCODED ANTIGENS IS DOMINATED BY CROSS PRESENTATION A. Busche, A.K. Gatzke, J. Zischke, J. Noack and M. Messerle KAPOSI SARCOMA-ASSOCIATED HERPESVIRUS INHIBITS A DNA REPAIR PATHWAY DURING LATENT INFECTION S. Cordes, B. Gruhne and T. F. Schulz Institute of Virology, Hannover Medical School, Hannover, Germany

08.30-10.00

W25 - Viral infections in transplant recipients

Chairs:

P. Griffiths, G. Gerna

Keynote Lecture: 08.30-09.00

P. Griffiths Control of cytomegalovirus in transplant patients

09:00-10:00

Oral presentations

Room B

ANTIVIRAL EFFECT OF CIPROFLOXACIN AND OFLOXACIN ON HUMAN POLYOMAVIRUS BK (BKV) REPLICATION IN PRIMARY HUMAN KIDNEY CELLS E. Bernhoff (1), B.S. Nath (1,2), T.J. Gutteberg (1,2) and C.H. Rinaldo (1) (1) Department of Microbiology & Infection Control, University Hospital of North Norway, Tromsø, Norway and (2) Department of Host-cell interactions, University of Tromsø, Tromsø, Norway. IMPACT OF INTRAHEPATIC HERPESVIRUS REPLICATION ON THE SURVIVAL OF LIVER TRANSPLANT RECIPIENTS J. Gösling (1), S. Pischke (2), I. Engelmann (1), B. Wölk (1), H. Wedemeyer (2), F. Lehner (3), T. Becker (3), H. Barg-Hock (3), C. Strassburg (2) and A. Heim (1) (1) Institute of Virology, (2) Department of Gastroenterology, Hepatology, and Endocrinology and (3) Department of General, Visceral and Transplant Surgery, Hannover Medical School, Hannover, Germany

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Scientific Programme – Sunday, April 11, 2010 VIROLOGIC AND IMMUNOLOGICAL MONITORING FOR MANAGEMENT OF HCMV INFECTION IN SOLID ORGAN TRANSPLANT RECIPIENTS. G. Gerna (1), D. Lilleri (2), P. Zelini (2), A. Chiesa (2), C. Pellegrini (3), E. Sarchi (4), C. Bigotto (5) and F. Meloni (6) (1) Laboratori Sperimentali di Ricerca, Area Trapiantologica, (2) Servizio diVirologia, (3) Divisione di Cardiochirurgia, (4) Clinica delle Malattie Infettive, (5) Dipartimento di Medicina Interna-Sezione di Nefrologia and (6) Clinica delle Malattie dell’Apparato Respiratorio, Fondazione IRCCS Policlinico San Matteo 27100 Pavia, Italia BREAK-THROUGH HHV-6B INFECTIONS DURING ANTIVIRAL PROPHYLAXIS AFTER LIVER TRANSPLANTATION I. Lautenschlager (1,2), R. Loginov (1,2), T. Karlsson (1,2) and K. Höckerstedt (2) (1) Department of Virology and (2) Department of Surgery, Division of Transplantation and Liver Surgery, and Transplant Unit Research Laboratory, Helsinki University Hospital and University of Helsinki, Finland.

08.30-10.00

W26 - Negative stranded RNA viruses

Chairs:

P. J. M. Rottier, L. Svensson

8.30-10.00

Oral presentations

Room C

A SINGLE AMINO ACID SUBSTITUTION IN THE MEASLES VIRUS F2 PROTEIN SUBUNIT ENHANCES FUSION ACTIVITY S. Heidmeier, A. Rasbach, I. Schneider, A. Sommer, C.J. Buchholz, K. Cichutek and M.D. Mühlebach. Division of Medical Biotechnology, Paul Ehrlich Institut, 63225 Langen, Germany EFFICACY ASSESSMENT OF COMMERCIALLY AVAILABLE AND CANDIDATE VACCINES AGAINST A PANDEMIC H1N1 2009 VIRUS G.P. Kobinger (1,2), I. Meunier (3), A. Patel (1,2), S. Pillet (3), J. Gren (1), S. Stebner (1), A. Leung (1), J.L. Neufeld (4), D. Kobasa (1,2) and V. von Messling (3) (1) Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada; (2) Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; (3) INRS-Institut Armand-Frappier, University of Quebec, Laval, Quebec, Canada and (4) National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada 3D STRUCTURE OF A NUCLEOPROTEIN-RNA COMPLEX OF RESPIRATORY SYNCYTIAL VIRUS AND SEARCH FOR THE P-BINDING DOMAIN R.G. Tawar (1), S. Duquerroy(1,2), M. Galloux (4), C. Vonrhein (3), P.F. Varela (1), L. Damier-Piolle(1), N. Castagné (4), K. MacLellan (5), H. Bedouelle (6), G. Bricogne (3), D. Bhella (5), F. A. Rey (1) and J.F. Eléouët (4) (1) Institut Pasteur, Unité de Virologie Structurale, Département de Virologie and CNRS URA 3015, Paris, France, (2) U. Paris-Sud, 91405 Orsay Cedex, France, (3) Global Phasing Ltd, Sheraton House, Castle Park, Cambridge, U. K., (4) INRA, VIM, 78350 Jouy-en-Josas, France, (5) MRC Virology Unit, University of Glasgow, U. K. and (6) Institut Pasteur, CNRS URA 3012, Paris, France MUTATION OF THE BINDING SITE BETWEEN TWO POLYMERASE SUBUNITS OF INFLUENZA A VIRUS LEADS TO SPONTANEOUS ESCAPE MUTANTS WITH RESTORED ACTIVITY V. Götz, B. Mänz, K. Wunderlich, D. Mayer and M. Schwemmle Department of Virology, University of Freiburg, Germany

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Scientific Programme – Sunday, April 11, 2010 PATHOGENESIS OF PANDEMIC AND ENDEMIC INFLUENZA VIRUS A/H1N1 IN PIGS D. Deckers (1), E. Lange(1), C. Maresch (1), A. Breithaupt (1), D. Kalthoff (2), M. Beer (2), T.C. Mettenleiter (3) and T.W. Vahlenkamp (1) (1) Institute of Infectology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (2) Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany and (3) Institute of Molecular Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany INFLUENCE OF NIPAH VIRUS INFECTION OF ENDOTHELIAL CELLS ON ADHESION MOLECULES S. Erbar and A. Maisner Institute of Virology, Philipps-University Marburg, Germany

08.30-10.00

W27 - Viral glycoproteins and entry

Chairs:

A. Alcami, U. F. Greber

8.30-10.00

Oral presentations

Room D

FLUORESCENCE SUPER-RESOLUTION MICROSCOPY IMAGING OF HIV ENV DISTRIBUTION AND ENV REARRANGEMENTS DURING THE VIRUS ENTRY J. Chojnacki (1), T. Staudt (2,3), P. Bingen (2), J. Engelhardt (2), S.W. Hell (2,3) and H.-G. Kräusslich (1) (1)Virology, Department of Infectious Diseases, University of Heidelberg, Heidelberg, Germany; (2)German Cancer Research Center (DKFZ), High-Resolution Optical Microscopy Division, Heidelberg, Germany and BIOQUANT, Heidelberg, Germany and (3) Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany EVIDENCE FOR SEPARATE gH/gL AND gH/gL/UL128-131 COMPLEXES IN HCMV VIRIONS THAT ARE BOTH REQUIRED FOR ENTRY INTO EPITHELIAL AND ENDOTHELIAL CELLS P.T. Wille, B.J. Ryckman, A.L. Vanarsdall, M.C. Chase, A.J. Knoche, M.A. Jarvis, J.A. Nelson and D.C. Johnson Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR, USA CHARACTERIZATION OF THE HERPES SIMPLEX VIRUS UPTAKE INTO HUMAN KERATINOCYTES P. Petermann (1), F. Rixon (2) and D. Knebel-Mörsdorf (1,3) (1) Centre for Biochemistry & (3) Department of Neurology, University of Cologne, Köln, Germany and (2) MRC Virology Unit, Glasgow, United Kingdom RECEPTOR INTERACTIONS AND ENTRY OF COXSACKIEVIRUS A9 T. Hyypiä (1), O. Heikkilä (1), P. Susi (1), T. Tevaluoto (1), V. Marjomäki (2) and S. Kiljunen (1) (1) Department of Virology, University of Turku and (2) Department of Biological and Environmental Science/Nanoscience Center, University of Jyväskylä, Finland HERPES SIMPLEX VIRUS TYPE I GLYCOPROTEINS B AND H FUNCTIONAL DOMAINS IN MEMBRANE FUSION M. Vitiello (1), A. Falanga (2), E. Finamore (1), A. Kampanaraki (1), S. Galdiero (2,3), C. Pedone (2,3) and M. Galdiero (1,3) (1) Department of Experimental Medicine - II University of Naples and (2) Department of Biological Sciences & (3) CIRPEB - University of Naples Federico II - Naples, Italy

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Scientific Programme – Sunday, April 11, 2010 INSERTION OF HERPES VIRUS GLYCOPROTEIN B FUSION PEPTIDES INDUCES TARGET MEMBRANE CURVATURE U. E. Maurer (1), T. Z. Ben Mordehai (5), J.T. Huiskonen (2,5), T.M. Cairns (3), B.P. Hannah (3,4), J. C. Whitbeck (3), R.J. Eisenberg (3), G.H. Cohen (3) and K. Grünewald (1,5) (1) Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, 82152, Germany, (2) Institute of Biotechnology, University of Helsinki, Helsinki, 00014, Finland, (3) Department of Microbiology, School of Dental Medicine, and Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104-6002, USA, (4) current address: Fox Chase Cancer Center, Philadelphia and (5) Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, UK

10.10-10.50

Plenary sessions ESV award (European Virology Award -EVA)

Chair:

O. Haller

Keynote Lecture:

P. Palese Pandemic Influenza Viruses: Past and Future

10.50-11.15

Coffee Break

11.15-12.45

W28 - Silencing of gene expression

Chairs:

S. Pfeffer, F. Cancellotti

Keynote Lecture: 11.15-11.45

S. Pfeffer Biological roles of virus-encoded microRNAs

11.45-12.45

Oral presentations

Room A

Room A

FUNCTIONAL MICRO RNA GENERATED FROM A CYTOPLASMIC RNA VIRUS H. Rouha (1), C. Thurner (1) and C.W. Mandl (1,2) (1) Medical University of Vienna, Institute of Virology, Vienna, Austria and (2) Current address: Novartis Vaccines and Diagnostics, Cambridge, Massachusetts, USA DETECTION OF INFLUENZA H1N1 MRNA IN SINGLE LIVING INFECTED MDCK CELLS USING FLUORESCENT FIT-PNAS AS SINGLE LABELLED HYBRIDIZATION PROBES S. Kummer (1), A. Knoll (2), O. Seitz (2) and A. Herrmann (1) (1) Department of Biology/Molecular Biophysics and (2) Department of Chemistry/Organic and Bioorganic Chemistry, Humboldt-University, Berlin, Germany 5’-TRIPHOSPHATED siRNA ACTIVATE RIG-I AND CONTROL HBV REPLICATION G. Ebert (1), P. Hendrik (2), H. Gunther (3) and U. Protzer (1) (1) Institute of Virology, Technische Universität München/Helmholtz Zentrum München, Munich, (2) Department of Internal Medicine III, University Hospital, TechnischeUniversitätMünchen, Munich and (3) Department of Clinical Pharmacology, University Hospital Bonn, Bonn, Germany THE NS3 PROTEIN OF RICE HOJA BLANCA TENUIVIRUS INTERFERES WITH THE MIRNA PATHWAY IN DISTINCT ORGANISMS E. Schnettler (1), H. Hemmes (1), W. deVries (2), R. Kormelink (1), B. Berkhout (2) and R. Goldbach (1) (1) Wageningen University, Virology, Wageningen, The Netherlands and (2) University of Amsterdam, Experimental Virology, Amsterdam, The Netherlands

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Scientific Programme – Sunday, April 11, 2010 10.50-12.45

W29 - Vaccines

Room B

Chairs:

A. Zanetti, T. F. Schwarz

Keynote Lectures:

A. Zanetti The global impact of vaccination against Hepatitis B: the state of the art T. Schwarz Immune response to human papillomavirus after prophylactic vaccination C. Liverani HPV vaccination: beyond cervical cancer

12.15-12.45

Oral presentations HIV VACCINE DESIGN TO IMPROVE GAG, POL, NEF AND ENV SPECIFIC IMMUNE RESPONSES K. Boeckl, J. Koestler, K. Kindsmueller, J. Wild and R. Wagner University of Regensburg, Institute of Medical Microbiology, Molecular Microbiology and Gene Therapy Unit, Regensburg, Germany VACCINE INDUCED T CELL IMMUNITY IS ESSENTIAL FOR RAPID PROTECTION AGAINST LETHAL ORTHOPOXVIRUS INFECTION M. Kremer (1)*, Y. Suezer (2)*, U. Kalinke (3) and G. Sutter (1) * Authors contributed equally to this work. (1) Institute for Infectious and Zoonotic Diseases, University of Munich, München, (2) Department of Virology, Paul-Ehrlich-Insitute, Langen, and (3) TWINCORE, Abteilung für Experimentelle Infektionsforschung, Hannover, Germany.

11.15-12.45

W30 - Endogenous retroviruses and neurovirology

Room C

Chairs:

M. Palmarini, A. Dolei

Keynote Lecture: 11.15-11.45

M. Palmarini Novel co-evolutionary mechanisms between endogenous retroviruses and their host

11.45-12.45

Oral presentations REGULATION OF HUMAN ENDOGENOUS RETROVIRUS (HERV) EXPRESSION IN HUMAN BRAIN CELLS O. Diem (1,2), W. Seifarth (2), O. Frank (2) and C. Leib-Mösch (1,2) (1) Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Virology, Neuherberg, Germany and (2) Medical Clinic III, University Medical Centre Mannheim, University of Heidelberg, Mannheim, Germany THE EPIGENETIC CONTROL OF HUMAN ENDOGENOUS RETROVIRUSES EMPLOYED IN TROPHOBLAST CELL FUSION J. Blazkova, K. Trejbalova, M. Matouskova, L. Pecnova and J. Hejnar Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czechia LIPID DERANGEMENT AND PRION MISFOLDING IN PRION-INFECTED MOUSE NEUROBLASTOMA CELL LINES AND IN BRAINS AND SKIN FIBROBLASTS FROM SCRAPIE-SUSCEPTIBLE SHEEP A. Pani (1), C.D. Orrù (1), M.D. Cannas (1), S. Vascellari (1), C. Abete (1), F. Angius (1), P.L. Cocco (1), C. Norfo (1), A. Mandas (2), P. La Colla (1) and S. Dessì (1) (1) Department of Biomedical Science & Technology and (2) Department of Internal Medicine, University of Cagliari, Italy.

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Scientific Programme – Sunday, April 11, 2010 DETECTION OF HUMAN ENDOGENOUS RETROVIRUS K (HERV-K) TRANSCRIPTS IN CELL LINES OF SQUAMOUS CELL CARCINOMA S. Grouls (1), V. Simon (2), E. Krautkrämer (1), A. Grebe (1), M. Sauter (3), N. MüllerLantzsch (3) and M. Zeier (1) (1) Department of Nephrology, University of Heidelberg, Heidelberg, Germany, (2) Department of Dermatology, University of Heidelberg, Heidelberg, Germany and (3) Institute of Virology, Saarland University Hospital, Homburg, Germany.

11.15-12.45

W31 - Viral infections in pregnancy

Room D

Chairs:

M.G. Revello, D. Wolf

Keynote Lecture: 11.15-11.45

M. G. Revello Diagnosis and management of primary Cytomegalovirus infection in pregnancy: A 20-year experience on 582 women

11.45-12.45

Oral presentations MOTHER-INFANT-PAIR SCREENING FOR CYTOMEGALOVIRUS INFECTION AT BIRTH IN GERMANY K. Hamprecht (1), H. Abele (2), K.-O. Kagan (2), S. Jürgens (1), K. Paukovic (1), D. Wallwiener (2), C. Poets (3), G. Jahn (1) and R. Goelz (3) (1) Institute for Medical Virology, (2) Dep. of Gynecology and Obstectrics and (3) Dep. of Neonatology, University Hospital of Tuebingen, 72076 Tuebingen, Germany ACTIVATION OF PPARγ BY HUMAN CMV FOR DE NOVO REPLICATION IMPAIRS INVASIVENESS OF CYTOTROPHOBLAST FROM EARLY PLACENTA B. Rauwel (1), B. Mariamé (1), H. Martin (1), D. Evain-Brion (2), T. Fournier (2) and C. Davrinche (1) (1) Inserm, U563,CHU Purpan, 31024, Toulouse, France and (2) Inserm, U767, 4 avenue de l’Observatoire, 75006, Paris, France A NOVEL EX VIVO ORGAN CULTURE MODEL OF HUMAN CYTOMEGALOVIRUS (HCMV) TRANSMISSION IN THE MATERNAL-FETAL INTERFACE Y. Weisblum (1,2), A. Panet (2), Z. Zakay-Rones (2), T. Shalem (1,3), S. Natanson-Yaron (3), R. Haimov-kochman (3), D. Goldman-Wohl (3), S. Yagel (3) and D.G. Wolf (1) (1) Clinical Virology Unit, (2) Department of Biochemistry and the Chanock center for Virology, IMRIC and (3) Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center & Faculty of Medicine, Jerusalem, Israel RUBELLA INFECTION AMONG PREGNANT WOMEN IN ITALY: HERD IMMUNITY STILL NOT ACHIEVED C. Galli (1) for the MIGHT (Multisite Italian Group for Hospital ToRC evaluation) (2) (1) Abbott Diagnostics, Roma and (2) Microbiology, Virology or Laboratory Departments: Hospital S. Orsola, Bologna; Annunziata Hospital of Cosenza, SS. Annunziata Hospital of Florence, Niguarda Hospital and Policlinico University Hospital of Milan, S. Gerardo Hospital of Monza, II Policlinico University Hospital of Naples, Policlinico Le Scotte University Hospital of Siena, Hospital of Scafati, Hospital of Sondrio, S. Giovanni Battista Hospital of Turin, G. Panico Hospital of Tricase, Italy.

12.45-13.00

CLOSING REMARKS

Room A

G. Campadelli-Fiume, C. De Giuli Morghen, G. Palù

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