Food Testing

Food Safety and Quality Diagnostics Bio-Rad Solutions

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Contents PART 1 • Routine Testing - Quality Indicator

3

Preparation of dilutions

3

Coliforms - ISO standards

6

Total flora

3

Coliforms - Alternative method: RAPID’E.coli 2 Agar

7

Escherichia coli - ISO standards

4

Enterobacteriaceae

8

Escherichia coli - Alternative method: RAPID’E.coli 2 Agar

5

Enterobacteriaceae - Alternative method: RAPID’Enterobacteriaceae 9

PART 2 • Pathogen Testing Salmonella - ISO standard

10 10

FDA-BAM Salmonella protocol 11

Listeria spp. - Alternative method: iQ-Check Listeria spp. real-time PCR Kit

22 23

FSIS Salmonella MLG 4.07

11

Salmonella - Alternative method: RAPID’Salmonella Agar double enrichment protocol

Escherichia coli O157:H7 - Alternative method: RAPID’E.coli O157:H7 Agar

12

Salmonella - Alternative method: RAPID’Salmonella Agar short protocol

STEC - iQ-Check STEC VirX and SerO real-time PCR Kits

24

12

Salmonella - Alternative method: iQ-Check™ Salmonella II real-time PCR Kit

Escherichia coli O157:H7 - Alternative method: iQ-Check E.coli O157:H7 real-time PCR Kit

24

12

Campylobacter spp. - ISO standard

25

Cronobacter spp. - ISO standard

14

FDA-BAM Campylobacter spp. protocol

26

FDA-BAM Cronobacter spp.

14

Campylobacter spp. - Alternative method: iQ-Check Campylobacter real-time PCR Kit

26

15

Cronobacter spp. - Alternative method: iQ-Check Cronobacter spp. real-time PCR Kit

Campylobacter spp. - Alternative method: RAPID’Campylobacter spp.

27

15

Staphylococcus aureus - ISO standards

28

Listeria monocytogenes - ISO standards

16

FDA-BAM Staphylococcus aureus 29

Cronobacter spp. - Alternative method: RAPID’Sakazakii Agar short protocol

FDA-BAM Listeria monocytogenes protocol 18 Health Canada Listeria monocytogenes protocol MFHPB-30 Listeria monocytogenes - Alternative method: RAPID’L.mono Agar Listeria monocytogenes - Alternative method: iQ-Check Listeria monocytogenes II real-time PCR Kit

18

29

Sulfite-reducing bacteria

30

Clostridium perfringens 30 19 20

Listeria spp. - Alternative method: RAPID’L.mono Agar 21 Listeria spp. - Alternative method: RAPID’Listeria spp. Agar

Staphylococcus aureus - Alternative method: RAPID’Staph Agar

Bacillus cereus

31

Shigella 31 Validations 32 Range for Food Testing

33

21

This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products. It is not a contractual document.

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Routine Testing • Quality Indicator

Preparation of dilutions

PCA

Bacteria

Ordering Information Catalog #

NF EN ISO 6887-1 (09/1999)

NF EN ISO 4833 (05/2003)

Preparation of test samples, initial suspension, and decimal dilutions for microbiological examination

General guidance for the enumeration of microorganisms

Part 1: General rules for preparation of dilutions for microbiological examination

Colony-count technique at 30°C

Description

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684

m g or v ml of sample + 9 x m g or 9 x v ml of Tryptone Salt or Buffered Peptone Water (and following dilutions)

Initial suspension and/or decimal dilutions

500 g

PCA (Plate Count Agar)/Agar Ready-to-use 355-4459 100 ml x 6 bottles 355-4457 200 ml x 6 bottles 356-3989 90 mm x 20 dishes Dehydrated 356-4475

Total flora

PCA

Dilution 10-1

500 g

Tryptone-Salt/Diluent Ready-to-use 355-5754 9 ml x 25 tubes 355-5756 90 ml x 6 bottles 355-5796 3 L x 4 bags Dehydrated 356-4544

30°C 72 hrs

500 g

(and following dilutions)

Enumeration

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Ordering Information Catalog #

Description

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684

500 g

EC Medium/Broth Dehydrated 356-4764 500 g Kovacs Reagent (for Indole detection) 355-5313 15 ml x 2 bottles Peptone Water (Indole Free)/Broth Ready-to-use 355-4180 9 ml x 25 tubes Dehydrated 356-4334

500 g

Escherichia coli - ISO standards ISO 16649-1 (04/2001)

Horizontal method for the enumeration Horizontal method for the enumeration of β-glucuronidase-positive E. coli of β-glucuronidase-positive E. coli Part 1: Colony-count technique at Part 2: Colony-count technique at 44°C using membranes and 44°C using 5-bromo-4-chloro5-bromo-4-chloro-3-indolyl 3-indolyl -D-glucuronide -D-glucuronide

Dilutions

Dilutions

TBX

MMGA with membrane Room t° 15 min and 37°C 4 hrs ± 1 hr

Resuscitation

(2 X)*

TBX 44°C 18-24 hrs

500 g

Direct enumeration

TBX/Agar Ready-to-use 355-5309 100 ml x 6 bottles 355-5307 200 ml x 6 bottles Dehydrated 356-4035

Sample/ initial suspension

Sample/ initial suspension

RAPID’E.coli 2/Agar Ready-to-use 355-5297 200 ml x 6 bottles 355-5299 100 ml x 6 bottles Dehydrated 356-4024

ISO 16649-2 (04/2001)

500 g

44°C 18-24 hrs

Direct enumeration

Tryptose Lauryl Sulfate/Broth Dehydrated 356-4774 500 g Tryptone-Salt/Diluent Ready-to-use 355-5754 9 ml x 25 tubes 355-5756 90 ml x 6 bottles 355-5796 3 L x 4 bags Dehydrated 356-4544

4 |

500 g

* Please refer to ISO 7218: 2007

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Routine Testing • Quality Indicator

Escherichia coli Alternative method: RAPID’E.coli 2 Agar ISO/TS 16649-3 (09/2005)

ISO 7251 (07/2005)

Horizontal method for the enumeration of β-glucuronidase-positive E. coli

General guidance for detection and enumeration of presumptive E. coli

Part 3: Most probable number technique using 5-bromo-4-chloro- Most probable number technique 3-indolyl -D-glucuronide

Sample/initial suspension

Add 10 ml in And subsequent dilution 3 tubes of double Add 1 ml in 3 tubes strength MMG of single strength MMG

Enumeration of E. coli NF VALIDATION according to ISO 16140 N° BRD 07/1-07/93 and BRD 07/7-12/04 AOAC-RI approved N° 050601** NordVal approved N° 020 Health Canada (Pending) All food stuffs

Sample/ initial suspension

Sample/ initial suspension

Dilutions

Tryptose Lauryl Sulfate

RAPID’E.coli 2

37°C 24 hrs ± 2 hrs Single concentration

Subculture each tube presenting yellow coloration on a dish of TBX 44°C 20-24 hrs

(or)*

Double concentration 44°C/37°C 18-24 hrs

37°C 24-48 hrs ± 2 hrs

37°C 24-48 hrs ± 2 hrs

EC Medium

Direct enumeration of typical colonies*

44°C 24-48 hrs ± 2 hrs

Each tube showing the presence of blue colonies on TBX should be considered positive

Peptone Water 44°C 48 hrs ± 2 hrs

Indole test reading and calculation Calculate the most probable number from the number of positive tubes at each dilution

* For detection method only

* Enumeration of typical colonies for E. coli at 44°C; for E. coli and coliforms at 37°C ** Please refer to AOAC certificate for validated matrices

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Coliforms - ISO standards ISO 4831 (08/2006) Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of coliforms VRBL

Coliforms

MPN technique

Ordering Information Catalog #

Description

Sample

BGBLB (Brilliant Green Bile Lactose Broth)/Broth Ready-to-use 357-8024 10 ml x 25 tubes

Dilutions

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684

Tryptose Lauryl Sulfate

Single concentration

500 g

RAPID’E.coli 2/Agar Ready-to-use 355-5297 200 ml x 6 bottles 355-5299 100 ml x 6 bottles Dehydrated 356-4024

30°C or 37°C 24-48 hrs ± 2 hrs

Double concentration 30°C or 37°C 24 hrs ± 2 hrs

500 g

Tryptose Lauryl Sulfate/Broth Dehydrated 356-4774 500 g

BGBLB

Tryptone-Salt/Diluent Ready-to-use 355-5754 9 ml x 25 tubes 355-5756 90 ml x 6 bottles 355-5796 3 L x 4 bags Dehydrated 356-4544

(or)*

30-37°C 24 hrs (+ 24 hrs)

500 g

Reading (gas +)

VRBL (Violet Red Bile Lactose)/ Agar Ready-to-use 355-4799 100 ml x 6 bottles Dehydrated 356-4594

6 |

* For detection method only

500 g

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Routine Testing • Quality Indicator

Coliforms Alternative method: RAPID’E.coli 2 Agar ISO 4832 (02/2006) Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coliforms

Enumeration of coliforms NF VALIDATION according to ISO 16140 N° BRD 07/8-12/04 AOAC-RI approved N° 050601** NordVal approved N° 020 Heath Canada (Pending) All food stuffs

RAPID’E.coli 2

Colony-count technique

Sample/ initial suspension Dilutions

E. coli

Other Coliforms

Total Coliforms

Sample/ initial suspension Dilutions

VRBL

RAPID’E.coli 2

(2 X)*

30-37°C 24 hrs ± 2 hrs

Enumeration of characteristic colonies and confirmation for atypical colonies

37°C 18-24 hrs

Direct enumeration of typical colonies*

Coliforms • Diameter ≥ 0.5 mm • Purple with or without a red zone of precipitated bile * Please refer to ISO 7218: 2007

* Enumeration of typical colonies for E. coli at 44°C; for E. coli and coliforms at 37°C ** Please refer to AOAC certificate for validated matrices

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Enterobacteriaceae ISO 21528-1 (08/2004) Microbiology of food and animal feeding stuffs Horizontal methods for the detection and enumeration of Enterobacteriaceae VRBG

Enterobacteriaceae

Ordering Information Catalog #

Part 1: Detection and enumeration by MPN technique with pre-enrichment

Description

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684 EE/Broth Dehydrated 356-4794

Sample Non-selective pre-enrichment

Buffered Peptone Water 37°C 18 hrs ± 2 hrs

500 g

EE 500 g

Nutrient (2.8%)/Agar Ready-to-use 355-4478 8 ml x 25 tubes Dehydrated 356-4484

500 g

Oxidase Test 355-3834

2 x vials of 50 oxidase disks

37°C 24 hrs ± 2 hrs

Selective enrichment

VRBG (Violet Red Bile Glucose)/ Agar Ready-to-use 355-4239 100 ml x 6 bottles Dehydrated 356-4584 500 g

VRBG 37°C 24 hrs ± 2 hrs

Confirmation Nutrient (2.8%) 37°C 24 hrs ± 2 hrs

Biochemical confirmation

Oxidase

Dextrose BCP 37°C 24 hrs ± 2 hrs

Yellow color (+)

8 |

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Routine Testing • Quality Indicator

Enterobacteriaceae Alternative method: RAPID’Enterobacteriaceae ISO 21528-2 (08/2004) Microbiology of food and animal feeding stuffs Horizontal methods for the detection and enumeration of Enterobacteriaceae

Enumeration of Enterobacteriaceae NF VALIDATION according to ISO 16140 N° BRD 07124-11/13

Enterobacteriaceae

RAPID’Enterobacteriaceae Non Enterobacteriaceae

Part 2: Colony-count technique

Sample/initial suspension Dilutions

Sample/ initial suspension Dilutions

RAPID’Enterobacteriaceae

37°C 24 hrs ± 2 hrs 37°C** ± 1°C 24 hrs ± 2 hrs

Reading

Confirmation Nutrient (2.8%)

Catalog #

Description

RAPID’Enterobacteriaceae/ Agar Ready-to-use 355-4012 200 ml x 6 bottles

VRBG (2 X)*

Ordering Information

Dehydrated 356-4004

500 g

Tryptone-Salt/Diluent Ready-to-use 355-5754 9 ml x 25 tubes 355-5756 90 ml x 6 bottles 355-5796 3 L x 4 bags Dehydrated 356-4544

500 g

Direct enumeration*

37°C 24 hrs ± 2 hrs

Biochemical confirmation

Oxidase (-)

Dextrose BCP 37°C 24 hrs ± 2 hrs

Yellow color (+) * Please refer to ISO 7218: 2007

* Suitable with an automatic plate counter ** Alternatively, a temperature of 30°C or 35°C can be chosen

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Salmonella - ISO standard EN ISO 6579/A1 (02/2006) Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp. Amendment 1 Appendix D: Detection of Salmonella spp. in animal feces and in samples from primary production stages

Sample Non-selective pre-enrichment

Buffered Peptone Water Selective enrichment

37°C ± 1°C 18 hrs ± 2 hrs

Appendix D Protocol

Rappaport Vassiliadis Soya

MSRV

MKTTn 37°C ± 1°C 24 hrs ± 3 hrs

41.5°C ± 1°C 24 hrs ± 3 hrs

41.5°C ± 1.5°C 24 hrs ± 3 hrs (+24 hrs for negative)

Plating-out Selective isolation

XLD

RAPID’Salmonella or XLT 4 or Kristensen or Hektoen

37°C ± 1°C 24 hrs ± 3 hrs

Confirmation Nutrient 2.1%

37°C ± 1°C 24 hrs ± 3 hrs

Confirmation

Biochemical and serological confirmation TSI Agar and other test

10 |

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Pathogen Testing

FDA-BAM Salmonella protocol

FSIS Salmonella MLG 4.07

Sample

Sample

Non-selective pre-enrichment

Non-selective pre-enrichment

Pre-enrichment depends on the matrix

Lactose Broth or Trypticase Soy Broth with/without Ferrous Sulfate or Universal Pre-enrichment Broth with/without Ferric Ammonium Citrate or Buffered Peptone Water 37°C 24 hrs ± 2 hrs

Selective enrichment

Rappaport Vassiliadis Soya

and

42°C 24 hrs ± 2 hrs

Tretiationate Broth

Buffered Peptone Water or mTSB (for raw beef, carcass, and environmental samples) 37°C 18-24 hrs

Selective enrichment

Modified Rappaport Vassiliadis Broth

Depending on the food

and

Tetrathionate Broth 42°C 22-24 hrs

35°C or 43°C 24 hrs ± 2 hrs

Plating-out Selective isolation

Plating-out Brillant Green Sulfat Agar

Selective isolation

XLD

Hektoen

and

XLT4 or DMLIA

Bismuth Sulfite 35°C 18-24 hrs

37°C ± 1°C 24 hrs ± 3 hrs

Confirmation Confirmation Biochemical and serological confirmation TSI and LIA Agar

Biochemical and serological confirmation TSI and LIA Agar

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Salmonella - Alternative method: RAPID’Salmonella Agar double enrichment protocol

Salmonella - Alternative method: RAPID’Salmonella Agar short protocol

Salmonella - Alternative method: iQ-Check Salmonella II real-time PCR Kit

Detection of Salmonella spp. NF VALIDATION according to ISO 16140 N° BRD 07/11-12/05 AOAC-RI approved N° 0507011* All food stuffs and feed

Detection of Salmonella spp. Certification by NF VALIDATION according to ISO 16140 N° 07/11-12/05 AOAC-RI approved N° 050701 NordVal approved N° 032

Detection of Salmonella spp. NF VALIDATION according to ISO 16140 N° BRD 07/06-07/04 AOAC-RI approved N° 010803* NordVal approved N° 038 Health Canada approved N° MFLP-38 All foods stuffs1,2, feed, and environment including primary production

Sample

Sample

Sample

Buffered Peptone Water Buffered Peptone Water

Buffered Peptone Water + 37°C 18 hrs ± 2 hrs

Non-selective pre-enrichment

Rappaport Vassiliadis Soya

Salmonella Capsule Supplement 41.5°C ± 1°C 18 hrs ± 2 hrs

Selective enrichment

41.5°C 6-26 hrs

Selective enrichment

37°C From 10 hrs ± 2 hrs to 21 hrs ± 1 hr

Bacterial Lysis & DNA Extraction (Standard or Easy)

Real-time PCR amplification and detection with iQ-Check Salmonella II

Plating-out Plating-out RAPID’Salmonella RAPID’Salmonella Selective isolation

37°C 24 hrs ± 2 hrs

Reading & confirmation Standard tests or Oxidase/OMNI-O/ONPG or iQ-Check Salmonella II

Selective isolation

37°C ±1°C 24 hrs ± 2 hrs

Reading & confirmation Salmonella Latex or Oxidase/ OMNI-O/ONPG or iQ-Check Salmonella

* Please refer to AOAC certificate for validated matrices

Automatic result analysis and interpretation

+/Confirmation of positive with RAPID’Salmonella * Please refer to AOAC certificate for validated matrices

For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative. 1 - For AOAC-RI protocol, please contact your local Bio-Rad sales representative 2 - 8-hr enrichment protocol available for raw meat (AOAC and AFNOR validated)

12 |

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Pathogen Testing

Salmonella spp.

RAPID’Salmonella Other Enterobacteriaceae

Ordering Information Catalog #

Description

Antiserum Salmonella Omnivalent OMNI-O (A60) 356-0781 3 ml dropper bottle

Novobiocin/Supplement Dehydrated 356-4610 1g

Brilliant Green (Kristensen)/Agar Dehydrated 356-4464 500 g

Nutrient (2.1%)/Agar Dehydrated 356-4485 500 g

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags

ONPG Test 355-3822 Set of 50 disks

Dehydrated 356-4684

500 g

Hektoen/Agar Ready-to-use 355-4386 100 ml x 6 bottles 356-3894 90 mm x 20 dishes Dehydrated 356-4284

500 g

iQ-Check Salmonella II Kit 357-8123 96 tests MKTTN (Muller Kauffmann Tetrathionate Novobiocin)/Broth Ready-to-use 355-6140 10 ml x 50 tubes Dehydrated Base 356-4714 500 g MSRV (Semi-Solid Rappaport Vassiliadis Medium) Ready-to-use 355-6139 200 ml x 6 bottles Dehydrated 356-4325

Oxidase Test 355-3834 2 x vials of 50 oxidase disks RAPID’Salmonella/Agar Ready-to-use 356-3961 90 mm x 20 dishes 356-3963 90 mm x 120 dishes Dehydrated 356-4705

500 g

RAPID´Salmonella Capsules/ Supplement 356-4709 100 x quantity for 2.5 L 356-4710 100 x quantity for 250 ml 356-4712 1 x quantity for 100 analyses RVS/Broth (Rappaport Vassiliadis Soy) Ready-to-use 355-5773 10 ml x 25 tubes Dehydrated 356-4324

Salmonella Confirm Latex Kit 355-6711 50 tests Selenite-Cystine/Broth Complete Medium 355-5746 10 ml x 25 tubes TSB/Broth Ready-to-use (containing novobiocin) 355-5426 225 ml x 6 bottles Dehydrated Base 356-4426 500 g TSI (Triple Sugar Iron)/Agar Dehydrated 356-4384 500 g XLD (Xylose Lysine Desoxycholate)/Agar Ready-to-use 354-1751 90 mm x 20 dishes Dehydrated 356-9124

500 g

XLT 4 (Xylose Lysine Tergitol 4)/Agar Ready-to-use 356-3654 90 mm x 20 dishes

500 g

Salmonella Latex Kit Detection of groups B to E and G 355-6710 75 tests

500 g Food Safety and Quality Diagnostics • Bio-Rad Solutions |

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Cronobacter spp. - ISO standard

Cronobacter spp.

ISO/TS 22964:2006 Milk and milk products Detection of Enterobacter sakazakii

FDA BAM Cronobacter spp. protocol Samples 36°C ± 1°C 24 hrs ± 2 hrs

Incubation

RAPID’Sakazakii Other Enterobacteriaceae

1:10 Buffered Peptone Water

Ordering Information Catalog #

Description

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684

40 ml 37°C 18 hrs ± 2 hrs

500 g

iQ-Check Cronobacter spp. 357-8137 96 tests RAPID’Sakazakii/Agar Ready-to-use 356-3971 90 mm x 20 dishes Dehydrated 356-4976

Sample preparation Weigh z g of the test portion in 9 x z ml Buffered Peptone Water

Selective enrichment Transfer 0.1 ml in 10 ml of mLST/vancomycin medium 44°C 24 hrs ± 2 hrs

500 g

Trypto-Casein-Soy (TSA)/Agar Ready-to-use 356-3884 90 mm x 20 dishes 355-5947 200 ml x 6 bottles 355-5944 8 ml x 25 inclined tubes

Centrifuge 3,000 x g for 10 min. Decant supernatant Resuspend pellet in 200 µl PBS

Streak onto RAPID’Sakazakii 44°C 24 hrs ± 2 hrs

Spread and streak plates 100 µl DFI Agar

R&F Agar

PCR PCR

Transfer 1 to 5 colonies on TSA Incubation 25°C 48 hrs

Perform identification tests on yellow colonies

14 |

36°C ± 1°C 18-24 hrs

Typical colonies from each plate are confirmed with real-time PCR and other techniques

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Pathogen Testing

Cronobacter spp. - Alternative method: RAPID’Sakazakii Agar short protocol

Cronobacter spp. - Alternative method: iQ-Check Cronobacter spp. real-time PCR Kit Detection of Cronobacter spp. Infant Formulas and environment NF VALIDATION pending

RAPID’Sakazakii short protocol NF VALIDATION according to ISO 16140 N° BRD 07/22-05/12

Sample preparation Weigh z g of the test portion in 9 x z ml BPW

37°C 18 hrs ± 2 hrs

Environmental sample

Buffered Peptone Water 37°C 18 hrs ± 2 hrs

Infant formula

Suppl. Buffered Peptone Water 37°C 20 hrs ± 2 hrs

Buffered Peptone Water 37°C 4 hrs ± 1 hr

Streak onto RAPID’Sakazakii 44°C 24 hrs ± 2 hrs

Bacterial Lysis & DNA Extraction (Standard or Easy)

Real-time PCR amplification and detection with iQ-Check Cronobacter spp. Transfer 1 to 5 colonies on TSA 25°C 48-72 hrs

Perform identification tests on yellow colonies

Automatic result analysis and interpretation

+/Confirmation of positive with RAPID’Sakazakii

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Listeria monocytogenes - ISO standards EN ISO 11290-1/A1 (02/2005) Horizontal method for the detection and enumeration of L. monocytogenes Part 1: Detection method

Sample

Fraser ½ Primary enrichment Isolation

Secondary enrichment

Fraser 1 (0.1 ml culture/10 ml)

Other solid selective medium

Agar Listeria according to Ottaviani and Agosti 37°C ± 1°C 24 hrs ± 3 hrs (+28 hrs ± 2 hrs)*

35°C or 37°C 48 hrs ± 2 hrs

30°C-35°C-37°C

Isolation

(depending of the media)

Agar Listeria according to Ottaviani and Agosti

24-48 hrs ± 2 hrs

Reading and confirmation

Listeria spp. -

TSYEA agar Catalase + Gram test Mobility

30°C 24 hrs ± 2 hrs

L. monocytogenes -

Other solid selective medium

37°C ± 1°C 24 hrs ± 3 hrs (+24 hrs ± 3 hrs)

TSYEB Hemolysis Carbohydrates CAMP test

30°C-35°C-37°C (depending on the media)

24-48 hrs ± 2 hrs

Reading and confirmation

* If low or absent colony growth observed after 24 hours.

16 |

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Pathogen Testing

Ordering Information Catalog #

EN ISO 11290-2/A1 (02/2005) Horizontal method for the detection and enumeration of L. monocytogenes

Description

AL (Agar Listeria according to Ottaviani and Agosti)/Agar Ready-to-use 356-3695 90 mm x 20 dishes 356-3965 90 mm x 120 dishes Dehydrated Base 356-4043 500 g

Part 2: Enumeration method

Base in vials (to be supplemented) 355-5200 237.5 ml x 6 bottles Supplement 1 356-4201 100 capsules (1 capsule qsp 250 ml) 356-4041 10 vials

Sample

Supplement 2 356-4042 10 vials

Dilution

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags

Fraser ½ without LiCl and selective supplement or Buffered Peptone Water 20°C ± 2°C 1 hr ± 5 min

Agar Listeria according to Ottaviani and Agosti 37°C 24 hrs ± 3 hrs (+ 24 hrs ± 3 hrs)

Enumeration and confirmation tests

Dehydrated 356-4684

500 g

Fraser ½/Broth Complete Medium 355-5797 225 ml x 6 bottles 355-5794 3 L x 4 bags 355-5792 5 L x 2 bags Dehydrated Base 356-4604 500 g Supplement 356-4616

10 vials

Fraser 1/Broth Complete Medium 355-4569 10 ml x 25 tubes Dehydrated Base 356-4604 500 g Supplement 356-4615

Listeria spp. -

TSYEA agar Catalase + Gram test Mobility

Listeria monocytogenes -

TSYEB Hemolysis Carbohydrates CAMP test

10 vials

Oxford/Agar Ready-to-use 356-3664 90 mm x 20 dishes Palcam/Agar Dehydrated Base 356-4754 500 g Supplement 356-4752

10 vials

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FDA-BAM Listeria monocytogenes protocol

Health Canada Listeria monocytogenes protocol MFHPB-30

Sample

Sample

BLED LEB 30°C 4 hrs 30°C 24 hrs

30°C 24 hrs

30°C 48 hrs

+ supplements 30°C 24 hrs ± 2 hrs (48 hrs ± 2 hrs)*

Oxford + RAPID’L.mono

Agar Listeria according to Ottaviani and RAPID’L.mono Agosti

Oxford or Palcam Modified Oxford or and (MOX)

37°C 24 hrs (+24 hrs)

0.1 ml MFB 35°C 24 hrs (48 hrs)

0.1 ml MFB 35°C 24 hrs (48 hrs)

Oxford + RAPID’L.mono

Oxford + RAPID’L.mono

35°C 24 – 48 hrs

35°C 24 – 48 hrs

or

37°C 24 hrs (+24 hrs)

35°C 24 – 48 hrs

Reading, isolation, and confirmation Real-time PCR amplification

Listeria spp. -

TSYEA agar Catalase + Gram test Mobility

L. monocytogenes -

TSYEB Hemolysis Carbohydrates CAMP test

Real-time PCR or amplification

Reading and confirmation and (optional) - Motility - Hemolysis - Mannitol, rhamnose and xylose (or other equivalent compendium-approved methods)

- Real-Time PCR Amplification - Catalase - Gram test - Serology

* If low or absent colony growth observed after 24 hours

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Pathogen Testing

Listeria monocytogenes - Alternative method: RAPID’L.mono Agar Detection of Listeria monocytogenes NF VALIDATION according to ISO 16140 N° BRD 07/4-09/98 AOAC-RI approved N° 0304061* NordVal approved All food stuffs and environment

Enumeration of Listeria monocytogenes NF VALIDATION according to ISO 16140 N° BRD 07/05-09/01 All food stuffs and environment

Sample

Sample Resuscitation

Fraser ½ Broth

Buffered Peptone Water

30°C 24 hrs ± 2 hrs

20°C 1 hr

Isolation on RAPID’L.mono 37°C 24 hrs ± 2 hrs

Reading and confirmation tests

Isolation on RAPID’L.mono 37°C 24 hrs ± 2 hrs

Direct enumeration

Standard Tests

Spot on iQ-Check Agar Listeria - TSYEB or according to or Listeria or Rhamnose - Hemolysis Test Ottaviani and monocytogenes II - Carbohydrates Agosti - CAMP test * Please refer to AOAC certificate for validated matrices

For a NF VALIDATION validated enumeration method, in the event of a positive result using RAPID’L.mono for enumeration, confirmation is not necessary when the presence of Listeria monocytogenes has been confirmed during detection.

For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative. 1-F  or AOAC-RI protocol, please contact your local Bio-Rad sales representative

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Listeria monocytogenes - Alternative method: iQ-Check Listeria monocytogenes II real-time PCR Kit Detection of Listeria monocytogenes NF VALIDATION according to ISO 16140 N° BRD 07/10-04/05 AOAC-RI approved N° 010802* NordVal approved N° 037 All food stuffs and environment

L. mono

L. ivanovii

RAPID’L.mono

L. innocua

Sample L. welshimeri

Ordering Information Catalog #

Description

(1)

Listeria Special Broth (LSB) or

(2)

Fraser ½ Broth

30°C 23 hrs ± 1 hr (1) or 25 hrs ± 1 hr (2)

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags

Bacterial Lysis & DNA Extraction Standard (Fraser ½ and LSB) or Easy (LSB)

Dehydrated 356-4684 500 g iQ-Check Listeria monocytogenes II Kit 357-8124 96 tests

Real-time PCR amplification and detection with iQ-Check Listeria monocytogenes II

LSB (Listeria Special Broth) Ready-to-use 355-5703 225 ml x 6 bottles 355-5793 5 L x 2 bags Dehydrated 356-4703 500 g 356-4753 5 kg RAPID’L.mono/Agar Ready-to-use 356-3964 90 mm x 120 dishes 356-3694 90 mm x 20 dishes 355-5294 Kit: 190 ml agar + 2 suppl.

Automatic result analysis and interpretation

+/-

Dehydrated Base 356-4293 500 g Supplement 1 356-4294 10 vials

Confirmation of positive with RAPID’L.mono

Supplement 2 356-4746 10 vials Rhamnose Test 355-3669 28 tests

20 |

* Please refer to AOAC certificate for validated matrices

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Pathogen Testing

Listeria spp. Alternative method: RAPID’L.mono Agar

Listeria spp. - Alternative method: RAPID’Listeria spp. Agar

Detection of Listeria spp. NF VALIDATION according to ISO 16140 N° BRD 07/4-09/98 All food stuffs and environment

Detection of Listeria spp. NF VALIDATION according to ISO 16140 N° BRD 07/12-12/06 AOAC-RI approved N° 080701* All food stuffs and environment

Sample

Sample

Fraser ½ Broth

Fraser ½ Broth or UVM 30°C 24 hrs ± 2 hrs

30°C 24 hrs ± 2 hrs

Isolation on RAPID’Listeria spp. Isolation on RAPID’L.mono 37°C 24 hrs ± 2 hrs

37°C (24 hrs) 48 hrs

Reading and confirmation tests

Reading and confirmation tests

Standard Tests - TSYEA - Gram test - Catalase

or iQ-Check

Listeria spp.

Standard Tests - Gram test - Catalase

Spot on

or RAPID’L.mono or Spot on Palcam or iQ-Check + Gram test + Catalase

Listeria spp.

* Please refer to AOAC certificate for validated matrices

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Listeria spp. - Alternative method: iQ-Check Listeria spp. real-time PCR Kit Detection of Listeria spp. NF VALIDATION according to ISO 16140 N° BRD 07/13-05/07 AOAC-RI approved N° 090701* Health Canada Validation - MFLP-39 All food stuffs and environment

Ordering Information Catalog #

Description

Fraser ½/Broth Ready-to-use 355-5797 225 ml x 6 bottles 355-5794 3 L x 4 bags 355-5792 5 L x 2 bags Dehydrated Base 356-4604 500 g Supplement 356-4616 10 vials

Sample

Listeria Special Broth (LSB) 30°C 23 hrs ± 1 hr (1) or 25 hrs ± 1 hr (2)

Bacterial Lysis & DNA Extraction ((1) Standard or (2) Easy)

Real-time PCR amplification and detection with iQ-Check Listeria spp.

iQ-Check Listeria spp. Kit 357-8113 96 tests LSB (Listeria Special Broth) Ready-to-use 355-5703 225 ml x 6 bottles 355-5793 5 L x 2 bags Dehydrated 356-4703 500 g 356-4753 5 kg RAPID’Listeria spp./Agar Dehydrated Base 356-4744 500 g Supplement 1 356-4745 10 vials Supplement 2 356-4746

10 vials

RAPID’L.mono/Agar Dehydrated Base 356-4293 500 g

Automatic result analysis and interpretation

+/Confirmation of positive with RAPID’Listeria spp. or RAPID’L.mono

Supplement 1 356-4294 10 vials (1 vial qsp 500 ml) Supplement 2 356-4746 10 vials (1 vial qsp 500 ml) For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative. *F  or AOAC-RI protocol, please contact your local Bio-Rad sales representative.

* Please refer to AOAC and Health Canada certificates for validated matrices

22 |

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Pathogen Testing

Escherichia coli O157:H7 - Alternative method: RAPID’E.coli O157:H7 Agar Detection of Escherichia coli O157:H7 NF VALIDATION according to ISO 16140 N° BRD 07/14-09/07 AOAC-RI approved N° 060701* All food stuffs and environment RAPID’E.coli O157:H7

E. coli O157:H7 Other E. coli

Ordering Information Catalog #

Description

Sample

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684

mTSB + Novobiocin

500 g

41°C 16-24 hrs

iQ-Check E.coli O157:H7 Kit 357-8114 96 tests

Immunomagnetic-separation

iQ-Check STEC SerO 357-8140 32 tests iQ-Check STEC VirX 357-8139 96 tests

Isolation on RAPID’E.coli O157:H7

mTSB/Broth Ready-to-use (containing novobiocin) 355-5426 225 ml x 6 bottles Base 356-4426

500 g 37°C 24 hrs ± 2 hrs

Novobiocin/Supplement Dehydrated 356-4610 1g

Reading and confirmation tests Standard tests or Latex or iQ-Check E.coli O157:H7

RAPID’E.coli O157:H7/Agar Base 356-4748 100 g SEB (STEC Enrichment Broth)/Broth 356-4001 500 g 356-4002 5 kg For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.

RAPID’E.coli O157:H7 can also be used as a second medium in the ISO 16654 standard * Please refer to AOAC certificate for validated matrices * For AOAC-RI protocol, please contact your local Bio-Rad sales representative.

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STEC iQ-Check STEC VirX and SerO real-time PCR Kits

Escherichia coli O157:H7 - Alternative method: iQ-Check E.coli O157:H7 real-time PCR Kit

USDA No Objection Letter

Detection of Escherichia coli O157:H7 NF VALIDATION according to ISO 16140 N° BRD 07/15-06/08 Raw beef AOAC-RI approved N° 020801* Raw ground beef, fresh spinash, apple cider

Sample

Sample

Detection and Isolation of non-O157 Shiga Toxin-Producing E. coli (STEC) from ground beef

SEB enrichment

Buffered Peptone Water

41.5°C 10-15 hrs

41.5°C 8-24 hrs

Bacterial Lysis & DNA Extraction Bacterial Lysis & Easy DNA Extraction Real-time PCR amplification and detection with iQ-Check STEC VirX (Automatic results analysis and interpretation)

Real-time PCR amplification and detection with iQ-Check E.coli O157:H7

Real-time PCR amplification and detection with iQ-Check STEC SerO (Automatic results analysis and interpretation) Result analysis and interpretation

+/+/Confirmation of posiitve with RAPID’E.coli O157:H7 * For AOAC-RI protocol, please contact your local Bio-Rad sales representative.

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Pathogen Testing

Campylobacter spp. - ISO standard ISO 10272-1:2006

ISO/TS 10272-2:2006

Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp.

Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp.

Part 1: Detection method

Part 2: Enumeration method

Sample

Sample Dilution

Bolton Broth

Isolation 0.1 ml mCDD (x2)

(microaerobic 37°C + 41.5°C 44 ± 4 hrs atmosphere) 4-6 hrs

41.5°C 40-48 hrs

Innoculate a steril loop

(microaerobic atmosphere)

Select 5 colonies mCDD Agar

Other solid selective medium

Isolation Columbia blood Agar

(microaerobic 41.5°C 44 ± 4 hrs atmosphere)

41.5°C 24-48 hrs

Reading and confirmation Isolation Columbia blood Agar 41.5°C 24-48 hrs

Examination of morphology and motility

Reading and confirmation

Examination of morphology and motility Study of growth Columbia blood Agar

(microaerobic 25°C 44 ± 4 hrs atmosphere)

Study of growth Columbia blood Agar

41.5°C 44 ± 4 hrs

Study of growth Columbia blood Agar

(microaerobic 25°C 44 ± 4 hrs atmosphere)

Study of growth Columbia blood Agar

41.5°C 44 ± 4 hrs

(aerobic atmosphere)

Oxidase

(aerobic atmosphere)

Oxidase

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FDA-BAM Campylobacter spp. protocol Most samples (except: shellfish, milk, frozen dairy products)

25 g sample (50 g vegetables)

100 ml Bolton Broth with antibiotics in net-lined bag

Campylobacter spp. - Alternative method: iQ-Check Campylobacter spp. real-time PCR Kit Detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari AOAC-RI approved N° 031209* For chicken carcass rinses, turkey swabs and raw ground chicken

Food Sample Suppl. Bolton Broth

Rinse gently; shake 5 min. (25 rpm on bench-top shaker) Remove net-lined bag

or

Microaerobic Except: bubbling at 30°C)

37°C 4 hrs

Microaerobic

37°C ± 1°C 41.5°C ± 1°C + 4 – 6 hrs 20 – 24 hrs

42°C 24 hrs

Bacterial Lysis and DNA Extraction (Easy)

Preenrichment

Microaerobic

Carcass rinse or sponge 2X BF-BEB

37°C 4 hrs

Real-time PCR amplification and detection with iQ-Check Campylobacter

Enrichment

42°C 20-44 hrs

Streak 24 and 48 hrs enrichments on isolation agar Result analysis and interpretation Abeyta-Hunt-Bark agar or mCCDA Incubate 37°C or 42°C* 24-72 hrs

+/-

* in microaerobic

Identification

26 |

| Food Safety and Quality Diagnostics • Bio-Rad Solutions * For AOAC-RI protocol, please contact your local Bio-Rad sales representative.

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Pathogen Testing

Campylobacter spp. - Alternative method: RAPID’Campylobacter spp. Detection of Campylobacter spp.

Enumeration of Campylobacter spp. NF VALIDATION according to ISO 16140 N° BRD 07/25–01/14 RAPID’Campylobacter

Campylobacter spp.

Ordering Information Sample enrichment

Suspension/Dilution

Preston Broth 41.5 °C ± 1°C 24 hrs ± 2 hrs

Isolation on RAPID’Campylobacter (microaerobic atmosphere)

41.5 °C ± 1°C 24-48 hrs

Reading and confirmation tests Campylobacter Confirm Latex or iQ-Check Campylobacter or Standard tests

Catalog #

Description

Buffered Peptone Water/Broth Ready-to-use 355-4179 225 ml x 6 bottles 355-5795 3 L x 4 bags 355-5790 5 L x 2 bags Dehydrated 356-4684 500 g

Isolation on RAPID’Campylobacter

(microaerobic atmosphere)

41.5 °C ± 1°C (24 hrs) 48 hrs

Reading and confirmation tests Campylobacter Confirm Latex or iQ-Check Campylobacter or Standard tests

Confirmation 356-3784 Columbia + Sheep Blood/Agar, 90 mm x 20 dishes 355-4164 Physiologic Sterile Water (0.85%), 9 ml x 25 tubes 355-3834 Oxidase test, 2 x 50 disks 355-4154 Sterile Distilled Water, 9 ml x 25 tubes 356-4297  Campylobacter Confirm Latex kit, 50 tests iQ-Check Campylobacter/Kit 357-8135 96 tests RAPID’Campylobacter/Agar Dehydrated Base 356-4295 500 g Supplement 356-4296 10 vials (1 vial qsp 400 ml) Tryptone Salt/Diluent Ready-to-use 355-5756 90 ml x 6 bottles 355-5754 9 ml x 25 tubes 355-5747 9 ml x 100 tubes 355-5796 3 L x 4 bags Dehydrated 356-4544 500 g

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Staphylococcus aureus - ISO standards NF EN ISO 6888-1/A1 (01/2004)

NF EN ISO 6888-2/A1 (12/2003)

NF EN ISO 6888-3 (06/2003)

Horizontal method for the enumeration of coagulase-positive Staphylococci (S. aureus and other species)

Horizontal method for the enumeration of coagulase-positive Staphylococci (S. aureus and species)

Horizontal method for the enumeration wof coagulase-positive Staphylococci (S. aureus and species)

Technique including confirmation of colonies

Technique using rabbit plasma fibrinogen agar medium

Detection and MPN technique for low numbers

Sample/initial suspension and following dilution

Sample/initial suspension and following dilution

Sample/initial suspension Giolitti and Cantoni Giolitti and Cantoni Broth Broth (or)* Simple concentration Double concentration

Baird-Parker

37°C 24 hrs ± 2 hrs (+24 hrs ± 2 hrs)*

Baird-Parker for RPF + RPF sup.

(2 X)* 35°C or 37°C 24 hrs ± 2 hrs (+ 24 hrs ± 2 hrs)**

(2 X)*

Baird-Parker

Enumeration

Confirmation Brain-Heart Infusion 35°C or 37°C 24 hrs ± 2 hrs

35°C or 37°C 18-24 hrs and 48 hrs

Direct enumeration of typical coagulase-positive Staphylococci

Confirmation of typical colonies on BHI

Direct enumeration of typical coagulasepositive colonies

Rabbit Plasma

Typical Staphylococci grey to black colonies with a surrounding clear halo and a white opaque zone

28 |

37°C 24-48 hrs

37°C 24 hrs

Coagulation reading

* Please refer to ISO 7218: 2007 ** If low or absent colony growth observed after 24 hours.

Baird-Parker for RPF + RPF sup.

37°C 24-48 hrs

Rabbit Plasma 35°C or 37°C 4-6 hrs or 24 hrs

or

37°C 4-6 hrs or 24 hrs

Reading

* Please refer to ISO 7218: 2007

* For detection method only * If low or absent colony growth observed after 24 hours.

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Pathogen Testing

Staphylococcus aureus Alternative method: RAPID’Staph Agar

FDA BAM Staphylococcus aureus Sample Dilutions Spread 1 ml on 3 plates Baird Parker

Enumeration of Coagulase-positive Staphylococci NF VALIDATION according to ISO 16140 N° BRD 07/9-02/05 AOAC-RI approved N° 080602* All food stuffs, feed and environment

Ordering Information Catalog #

Description

Additives Egg Yolk with Potassium Tellurite 355-4201 5 ml x 1 vial 355-4205 25 ml x 1 bottle Sulfamethazine Solution (0.2%) 356-2682 2.5 ml x 1 vial

Sample/initial suspension

Baird-Parker/Agar Complete Medium 356-3991 90 mm x 20 dishes Base 356-4814

35°C 45-48 hrs

Confirmation RAPID’Staph 37°C 24 hrs ± 2 hrs

Enumeration and Confirmation

500 g

Baird-Parker for RPF/Agar Complete Medium 357-8618 90 ml x 6 bottles + 6 vials of suppl. 356-3996 90 mm x 20 dishes Dehydrated Base 356-4814 500 g RPF Supplement 356-4618 10 vials BHI (Brain Heart Infusion)/Broth Ready-to-use 355-3664 10 ml x 25 tubes

Pastorex™ Staph Plus

Spot on

or Baird-Parker for RPF

Dehydrated 356-4014

500 g

Pastorex Staph Plus Latex 355-6356 50 tests 355-6353 5 x 50 tests ™

Rabbit Plasma Freeze-dried 355-6352 Pack for 20 reactions Special broth 355-3544

* For AOAC-RI protocol, please contact your local Bio-Rad sales representative * Please refer to AOAC certificate for validated matrices

9 ml x 25 tubes

RAPID’Staph/Agar Ready-to-use 356-3960 90 mm x 20 dishes Base 356-4704

500 g

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Sulfite-reducing bacteria

TSC

Clostridium perfringens

Ordering Information Catalog #

Description

Lactose Sulfite/Broth Base 356-4914 100 g

ISO 15213 (05/2003)

NF EN ISO 7937 (02/2005)

Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions

Horizontal method for the enumeration of Clostridium perfringens

Food sample/initial suspension

TSC without D-Cycloserine/Agar Base Medium Ready to use 355-4419 100 ml x 6 bottles Base 356-9644

TSC

(add D-cycloserine) (2 X)*

Sheep Blood Supplement 355-6652 50 ml vial

500 g

Food sample/initial suspension

Dilution

Dehydrated Base 356-4674 500 g

Dehydrated 356-4084

Colony-count technique

Heat treatment 20 min 75°C

Sheep Blood/Agar Ready to use 356-3784 90 mm x 20 dishes

Thioglycolate + Resazurin/Broth Ready to use 356-3613 10 ml x 25 tubes

Clostridium perfringens

TSC

Anaerobes condition

(2 X)*

Thermophilous bacteria suspicion 50°C 24-48 hrs

37°C 24-48 hrs anaerobic condition

Enumeration of typical black colonies Confirmation of 5 isolated colonies with Thioglycolate + Resazurin 37°C 18-24 hrs

Anaerobes condition

Reading

500 g

Lactose Sulfite Confirmation

* Please refer to ISO 7218: 2007

Mobility Nitrate

46°C 24 hrs Clostridium perfringens (black precipitate/ gas+)

30 |

37°C 20 hrs ± 2 hrs

Clostridium perfringens (red coloration/ gas+)

Lactose Gelatine 37°C 18-24 hrs Clostridium perfringens (yellow/ gas+)

* Please refer to ISO 7218: 2007

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Pathogen Testing

Bacillus cereus

Shigella

NF EN ISO 7932 (07/2005)

NF EN ISO 21567 (03/2005)

General guidance for the enumeration of B. cereus

Horizontal method for the detection of Shigella spp.

Colony-count technique at 30°C

Mossel (MYP)

Bacillus cereus

Ordering Information Food sample/initial suspension

Food sample/initial suspension

Mossel (MYP)

Shigella Broth + Novobiocin pH 7 ± 0.2 41°C 16-20 hrs

(2 X)*

Plating-out

30°C 18-24 hrs (+24 hrs)

Confirmation test on 5 well isolated colonies

Mac Conkey

Reading and interpretation

Hektoen

Description

Hektoen/Agar Ready-to-use 355-4386 100 ml x 6 bottles 356-3894 90 mm x 20 dishes Dehydrated 356-4284

500 g

Mac Conkey + Crystal Violet/Agar Ready-to-use 356-3617 90 mm x 20 dishes Dehydrated 356-9084

500 g

Novobiocin/Supplement Dehydrated 356-4610 1g Nutrient (2.1%)/Agar Dehydrated 356-4485 500 g

37°C 20-24 hrs (+48 hrs)*

Sheep Blood/Agar 30°C 24 hrs ± 2 hrs

XLD

Catalog #

Spot on typical colonies on Nutrient (2.1%) 37°C 20-24 hrs

Mossel/Agar (Mannitol Egg Yolk Polymixin) Dehydrated 356-9604 500 g XLD (Xylose Lysine Desoxycholate)/Agar Ready-to-use 354-1751 90 mm x 20 dishes Dehydrated 356-9124

500 g

Biochemical and serological confirmation * Please refer to ISO 7218: 2007

* If low or absent colony growth observed after 24 hours.

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Validations PCR Methods iQ-Check Solutions AOAC

NF Validation

Health Canada

Chromogenic Methods RAPID’ Solutions NordVal

AOAC

NF Validation

Health Canada

NordVal

Campylobacter spp. Coliforms (pending)

Cronobacter spp. Escherichia coli (pending

Escherichia coli O157:H7 Enterobacteriaceae

Listeria monocytogenes

Detection (pending

Enumeration

Listeria spp. Salmonella Enteritidis Salmonella spp. Salmonella Typhimurium Staphylococcus aureus STEC 7 major O-groups* STEC virulence genes* * No objection letter delivered from USDA

32 |

| Food Safety and Quality Diagnostics • Bio-Rad Solutions

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Bio-Rad Solutions

Range for Food Testing Solution Segment Microorganism

Meat & Poultry & Fish Products

Water & Wine Beverages

Dairy Products

Environment

Processed Foods

Vegetables Produce

Bacillus cereus Campylobacter Clostridium perfringens Coliforms Cronobacter sakazakii Enterobacteriaceae Escherichia coli Escherichia coli O157:H7 STEC Lactobacillus Listeria spp. Listeria monocytogenes Pseudomonas aeruginosa Salmonella spp. Salmonella Enteritidis Salmonella Typhimurium Staphyloccocus aureus Yeasts and Molds

RAPID’Chromogenic method

iQ-Check PCR method

Classical media

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| Food Safety and Quality Diagnostics • Bio-Rad Solutions

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Real-Time PCR Methods • Food Testing

Start, Run, Results iQ-Check™ Prep Solution, when the power of automation meets real-time PCR • Reliable walk-away automation for real-time PCR testing • Designed to fit lab workflow without disrupting it • Increased confidence with improved reproducibility • Optimized to handle all major food pathogens For more information: foodscience.bio-rad.com

iQ-Check Prep, CFX96 Touch™ deep well and iQ-Check Kits

The Bio-Rad PCR Solution, Your Solution

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Life Science Group

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© Bio-Rad 10 17933 14-0686 Rev I 04/2014

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