ELECTROPHORESIS FISHER SCIENTIFIC >> LIFE SCIENCE
Gel Boxes for Protein Electrophoresis Hoefer® SE600 Series Vertical Electrophoresis Systems
Fisher Scientific is your One Source for Electrophoresis. We are proud to offer a complete line of electrophoresis products for all of your research needs. From reagents and precast gels to gels boxes and transilluminators, we have everything you need to perform reliable, reproducible electrophoresis of nucleic acids and proteins.
Built-in heat exchanger maintains temperature between 1° and 45°C for faster runs with superior quality results. System
Gel Size
SE600 Chroma SE600 Complete
18 x 16cm or 18 x 8cm 18 x 16cm or 18 x 8cm
Buffer Volume 5L 5L
Catalog No. 03-500-100 03-500-101
Each $1448.00 $1040.00
Hoefer Gel Casters for SE600 Vertical Electrophoresis Systems Cast multiple gels.
SE600 Chroma
Model
Plate Size
SE6015 Dual-Gel Caster SE675 Four-Gel Caster SE615 Multiple-Gel Caster
18cm wide 18 x 16cm 18 x 16cm
Max. No. of Gels 2 4 10
Catalog No. 03-500-247 03-500-268 03-500-260
Each $278.00 $723.00 $771.00
Hoefer MiniVE Mini Vertical Electrophoresis Unit
Accepts gels up to 10 x 10.5cm, increasing separation length by more than 30% over conventional 10 x 8cm gels. Mfr. No. SE300-10A-1.0
Gel Size 10 x 10.5cm or 10 x 8cm
Buffer Volume 1.4L
Catalog No. 03-500-108
Each $729.00
Includes three rectangular glass plates, three notched glass plates, two gel modules, lid, tank, two 1mm 10-well combs, and two 1mm spacer sets. Hoefer MiniVE Mini Vertical Electrophoresis Unit
Hoefer Blot Module for MiniVE Mini Vertical Electrophoresis Unit Can be used to transfer two minigels inside the MiniVE tank. Mfr. No. SE302
Gel W x L 10 x 10.5cm or 10 x 8cm
Catalog No. 03-500-109
Each $402.75
Includes three Dacron® sponges (1/4” thick) and 25 sheets of blotting paper..
Hoefer Gel Casters for MiniVE Mini Vertical Electrophoresis Unit Model SE245 Dual-Gel SE235 4-Gel
Plate Size 10 x 8cm or 10 x 10.5cm 10 x 10.5cm
No. of Gels 1-2 1-4
Catalog No. 03-500-114 03-500-115
Each $209.00 $337.00
Hoefer Blot Module for MiniVE Mini Vertical Electrophoresis Unit
Hoefer systems are compatible with gels from Pierce, Novex/Invitrogen, and Jule; and power supplies from Amersham and Consort.
Precast Gels for Protein Electrophoresis Pierce Precise Protein Gels TM
TM
Produce results in just 45 minutes! Ready-to-use precast polyacrylamide gels in a neutral pH buffer permit proteins to stain quickly and with high sensitivity using Coomassie and silver stains. Format 10-well
12-well
15-well
Gel Conc. 8% 10% 12% 8-16% 4-20% 8% 10% 12% 8-16% 4-20% 8% 10% 12% 8-16% 4-20%
Separation Range 45-205kDa 24-205kDa 14-205kDa 14-205kDa 6.5-205kDa 45-205kDa 24-205kDa 14-205kDa 14-205kDa 6.5-205kDa 45-205kDa 24-205kDa 14-205kDa 14-205kDa 6.5-205kDa
Well Volume 50µL 50µL 50µL 50µL 50µL 30µL 30µL 30µL 30µL 30µL 25µL 25µL 25µL 25µL 25µL
Catalog No. PI-25200 PI-25201 PI-25202 PI-25203 PI-25204 PI-25220 PI-25221 PI-25222 PI-25223 PI-25224 PI-25240 PI-25241 PI-25242 PI-25243 PI-25244
Pack of 10 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00 $102.00
Pierce 10-well Protein Gel
Reagents, Buffers, and Standards for Protein Electrophoresis Pierce Lane Marker Sample Buffers
Utilize a bright pink hydrophobic tracking dye for SDS-PAGE that transfers and permanently binds to nitrocellulose membranes. May be used to assess transfer efficiency and align cut membranes. Description Reducing Sample Buffer Nonreducing Sample Buffer
Size 5mL 5mL
Catalog No. PI-39000 PI-39001
Each $44.00 $41.00
Pierce BlueRanger and TriChromRanger Prestained Protein Molecular Weight Marker Mixes TM
TM
Yield single bands of uniform intensity. Can be efficiently transferred onto both nitrocellulose and PVDF membranes. Description BlueRanger Marker Mix TriChromRanger Marker Mix
Molecular Weight Range 18.3 to 210kDa
No. of Bands 7
Quantity 1 x 48 microtube plate
Catalog No. PI-26681
18.3 to 210kDa 16.5 to 210kDa
7 7
5 x 48 microtube plate 1 x 48 microtube plate
PI-26685 PI-26691
Each $91.00 $395.00 $115.00
Detergents Reagent SDS, 20% Soln
Size 200mL 1L 100g 500g 100mL 500mL 100mL 500mL
SDS, Powder Triton® X-100 Tween® 20
Catalog No. BP2436-200 BP2436-1 BP166-100 BP166-500 BP151-100 BP151-500 BP337-100 BP337-500
Each $40.62 $103.74 $36.80 $160.91 $22.52 $31.73 $18.41 $22.04
Case of 6/$221.77 6/$539.48 6/$205.79 6/$897.92 6/$125.18 6/$176.42 6/$104.01 6/$124.58
Each $51.63 $104.04
Case of 6/$279.76 6/$565.50
Pierce BlueRanger and TriChromRanger Prestained Protein Molecular Weight Marker Mixes
Stains and Dyes Reagent Bromophenol Blue, Free Acid Bromophenol Blue, Sodium Salt
Size 25g 25g
Catalog No. BP115-25 BP114-25
Fisher BioReagents Buffers and Reagents for Protein Electrophoresis Acrylamide, Bis-Acrylamide, and Catalysts Reagent Acrylamide
Acrylamide Solution, 40% Acrylamide: Bis-Acrylamide 19:1 Powder Acrylamide: Bis-Acrylamide 29:1 Powder Acrylamide: Bis-Acrylamide 37.5:1 Powder Acrylamide: Bis-Acrylamide 19:1, 40% Soln. Acrylamide: Bis-Acrylamide 29:1, 40% Soln. Acrylamide: Bis-Acrylamide 37.5:1, 40% Soln. Bis-Acrylamide Bis-Acrylamide Solution, 2% w/v Ammonium Persulfate TEMED
Size 100g 500g 5kg 1L 100g 100g 100g 1L 1L 1L 25g 100g 250mL 25g 100g 26mL (20g) 130mL (100g)
Catalog No. BP170-100 BP170-500 BP170-5 BP1402-1 BP1364-100 BP1366-100 BP1368-100 BP1406-1 BP1408-1 BP1410-1 BP171-25 BP171-100 BP1404-250 BP179-25 BP179-100 BP150-20 BP150-100
Each $61.81 $173.61 $1169.13 $152.80 $60.22 $61.43 $61.43 $182.92 $176.50 $179.24 $39.56 $93.17 $39.93 $23.12 $31.15 $36.13 $107.23
Case of 6/$342.80 6/$1014.73 ----------------6/$232.86 6/$506.56 6/$211.63 6/$128.05 6/$172.66 6/$200.17 6/$583.04
For a complete listing of precast gels, gel boxes, and electrophoresis reagents, go to:
www.fishersci.com/lifescience/electrophoresis
PAGEgel Cassette Gels and Reagents TM
PAGEgel Cassette Gels
Unique acrylamide polymer permits exceptional protein separations and produces a TM super-strong gel for easy handling. 10 x 10cm gels fit in all XCell existing electrophoresis TM units from Invitrogen .
For SDS PAGE Format 12-well
17-well
2D-well
Gel Conc. 8% 10% 12% 16% 4-8% 4-12% 4-20% 10-20% 8% 10% 12% 16% 4-8% 4-12% 4-20% 10-20% 4-20%
Well Volume 35µL 35µL 35µL 35µL 35µL 35µL 35µL 35µL 17µL 17µL 17µL 17µL 17µL 17µL 17µL 17µL 300µL
Catalog No. CK00812 CK01012 CK01212 CK01612 CK40812 CK41212 CK42012 CK82012 CK00827 CK01027 CK01227 CK01627 CK40827 CK41227 CK42027 CK82027 CK42002
Each $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11 $10.11
Pack of 10 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00
PAGEgel Cassette Gels
For DNA and Native Protein PAGE Format 12-well
17-well
Gel Conc. 10% 2-8% 3-20% 10% 2-8% 3-20%
Well Volume 35µL 35µL 35µL 17µL 17µL 17µL
Catalog No. DN01012 DN20812 DN32012 DN01027 DN20827 DN32027
Each $10.11 $10.11 $10.11 $10.11 $10.11 $10.11
Pack of 10 $100.00 $100.00 $100.00 $100.00 $100.00 $100.00
PAGEgel Buffers and Solutions For use with PAGEgel Cassette Gels. Description LDS Sample Buffer, 5X SDS Run Buffer, 20X DNA/Native Sample Buffer, 4X DNA/Native Run Buffer, 20X
Application For SDS PAGE For SDS PAGE For DNA and Native Protein PAGE For DNA and Native Protein PAGE
Size 8mL 500mL 10mL 500mL
Catalog No. CB35008 CB60500 DB33010 DB61500
Each $12.11 $55.41 $12.11 $55.41
Related Equipment FisherBiotech Power Supplies
FisherBiotech Gel Drying System
FisherBiotech Power Supplies
Easy-to-use power supplies are designed for safety. Type Compact High Current Programmable Programmable
Maximum Voltage 300V 200V 1000V 3000V
Maximum Current 400mA 2.0A 500mA 400mA
Maximum Power 75w 200w 250w 400w
No. of Jacks 3 4 4 4
Internal Timer 0-999 min. 99 hr. 59 min. 99 hr. 59 min. 99 hr. 59 min.
Catalog No. FB300 FB200 FB1000 FB3000
Each $405.85 $822.01 $1088.45 $1872.26
FisherBiotech Gel Drying System
Fast gel drying—without distortion or cracking. Integrates a gel dryer and vacuum system to provide a simple, effective, and reliable way to dry electrophoresis gels, while eliminating the hassles of dry ice and refrigerated traps. Dried gels are suitable for autoradiography, densitometry, or notebook storage. Description Gel Drying System Gel Dryer Only
Electrical Requirements 115V 60Hz 115V 50/60Hz
Catalog No. FB-GD-X1 FB-GD-45-10
Each $3167.71 $1483.63
Spectroline® Microprocessor-Controlled UV Crosslinker Spectroline Microprocessor-Controlled UV Crosslinker
Covalently binds nucleic acids to membranes in 30 seconds or less following Southern, Northern, or dot blotting. Model Gel Drying System
Chamber Dimensions 17.5 x 13.5 x 7” (19 x 34 18cm)
Includes Five 8w, 254nm UV tubes
Catalog No. 11-992-89
Each $1472.21
Gel Boxes for DNA Electrophoresis FisherBiotechTM Horizontal Electrophoresis Systems
Choose from four systems that accommodate a variety of gel sizes. System Minigel Midigel Large Gel Wide Format
Gel Size 7 x 10cm 13 x 16cm 20 x 25cm 18 x 23cm
Buffer Capacity 400mL 800mL 2.3L 1.2L
LxWxH 16 x 9.5 x 8cm 24.5 x 18 x 8cm 37.5 x 28 x 10cm 33 x 27 x 13
Catalog No. FB-SB-710 FB-SB-1316 FB-SB-2025 FB-SB-2318
Each $346.22 $462.13 $560.83 $603.93
Models with buffer recirculation feature also available. Please visit www.fishersci.com for details.
FisherBioTech Horizontal Electrophoresis Systems
Owl EasyCastTM Horizontal Minigel Systems
Designed for simple use with high performance results. Randomly available in yellow, orange, and green. Gel Size 7 x 8cm 9 x 11cm 12 x 14cm
Buffer Capacity 400mL 600mL 800mL
Catalog No. 09-528-110 09-528-178 09-528-110B
Each $313.52 $350.59 $403.40
Owl EasyCast Horizontal Minigel Systems
DNA Molecular Weight Markers Fisher BioReagentsTM exACTGene® DNA Ladders
Ready-to-use ladders are stable at room temperature—no need to thaw and refreeze. Specially engineered to provide blunt-end DNA of precise length for better band quality than classic markers. Ideal for size determination and quantitative determination of DNA mass. Contain loading dye to reduce pipetting steps and save time. Marker 50 bp Mini Ladder 100 bp PCR DNA Ladder Low Range DNA Ladder 100 bp DNA Ladder
Fisher BioReagents exACTGene DNA Ladders (BP2570-100)
Low Range Plus DNA Ladder Cloning DNA Ladder Mid Range DNA Ladder Mid Range Plus DNA Ladder 1kb DNA Ladder 1kb Plus DNA Ladder 24kb Max DNA Ladder
No. of Base Pair Range Bands Application 25 to 650 bp 14 PCR Size Confirmation 25 to 1000 bp 12 PCR Size Confirmation 50 to 2000 bp 8 Quick Check of PCR or Digestion 100 to 1000 bp 10 General Purpose, Small Size DNA 100 to 2000 bp 11 General Purpose, Small Size DNA 100 to 2686 bp 14 Clone Identification 300 to 5000 bp
10
100 to 5000 bp
16
300 to 10,000 bp
13
100 to 10,000 bp
19
300 to 24,000 bp
15
Large Size PCR or Cloning Small and Large Cloning Applications General Purpose, Large Digested DNA General Purpose, Wide Size Range General Purpose, Extra Large Sizes
Size 1000µL
Catalog No. BP2570-100
Each $117.71
1000µL
BP2571-100
$117.71
1000µL
BP2572-100
$117.71
1000µL
BP2573-100
$117.71
1000µL
BP2574-100
$117.71
1000µL
BP2575-100
$117.71
1000µL
BP2576-100
$117.71
1000µL
BP2577-100
$117.71
1000µL
BP2578-100
$117.71
1000µL
BP2579-100
$117.71
1000µL
BP2580-100
$117.71
ABgene® Molecular Weight Markers
Supplied in storage buffer with a separate vial of 6X Type II Gel Loading Buffer to add to samples.
ABgene Lambda EcoR I/Hind III Marker
Marker Lambda EcoR I/Hind III
Base Pair Range 125 to 21,226 bp
No. of Bands 13
Lambda Hind III
125 to 23,130 bp
8
pHC624 Taq I/pMJ3 Nci I
96 to 1444 bp
8
Size 100µg 500µg 100µg 500µg 40µg
Catalog No. AB-0199 AB-0199B AB-0200A AB-0200B AB-0198
Each $43.39 $156.88 $40.36 $145.82 $60.45
The Polymerase Chain Reaction (PCR) process is covered by foreign counterparts of U.S. Patents 4,683,202; 4,683,195; and 4,965,188 owned by F. Hoffmann-La Roche Ltd.
Reagents and Buffers for DNA Electrophoresis Fisher BioReagents Stains
Fisher BioReagents Agarose Agarose Type Low EEO, Multipurpose, Molecular Biology Grade Medium EEO, Protein Electrophoresis Grade High EEO, Protein Electrophoresis Grade Genetic Analysis Grade Molecular Biology Grade (Low Melting) For >1000 bp DNA Separation PCR Grade, Intermediate Melting Protein Electrophoresis Grade (High Gelling Temperature)
Size 100g
Catalog No. BP160-100
Each $138.71
Case of 6/$752.19
500g 100g 100g 100g 500g 100g
BP160-500 BP161-100 BP162-100 BP1356-100 BP1356-500 BP1360-100
$456.58 $187.92 $195.78 $200.38 $800.28 $324.66
6/$2490.38 6/$1001.88 6/$1054.22 6/$1086.59 --6/$1775.58
100g 100g
BP2410-100 BP164-100
$286.66 $192.71
6/$1575.90 6/$1039.22
500g
BP164-500
$662.79
6/$3615.20
Reagent Ethidium Bromide, Powder Ethidium Bromide, 1% Soln.
Size 5g 10mL
Catalog No. BP102-5 BP1302-10
Each $116.71 $41.12
Case of 6/$644.06 ---
Fisher BioReagents Running Buffers, Transfer Buffers, and Components Reagent Saline-Sodium Citrate (SSC), 20X Soln. Tris Base, Crystalline Powder Tris-Acetate-EDTA, 50X Soln. Tris-Acetate-EDTA, 10X Soln. Tris-Borate-EDTA, 10X Soln. Tris-Glycine, 10X Soln.
Size 1L 4L 1kg 10kg 1L 4L 1L 4L 4L 20L 1L
Catalog No. BP1325-1 BP1325-4 BP152-1 BP152-10 BP1332-1 BP1332-4 BP1335-1 BP1335-4 BP1333-4 BP1333-20 BP1306-1
Each $59.96 $108.44 $87.27 $556.30 $91.86 $174.23 $44.42 $89.24 $86.80 $274.46 $61.56
Case of 6/$343.06 --4/$325.22 --6/$525.78 --6/$235.44 ------6/$352.34
ABgene Gel Loading Buffers
Contain a precipitant to ensure that samples sink into wells and a tracking dye to follow the progress of electrophoresis. Description 10X ReddyRunTM Gel Loading Buffer 6X Type II Gel Loading Buffer
Size 5mL 5mL
Catalog No. AB-0594 AB-0965
Each $40.36 $58.49
Related Equipment FisherBiotech Ultraviolet Transilluminators
Variable-intensity transilluminators with high-intensity 312nm tubes. Continuously adjustable from 100 to 20% intensity. Filter Area 20 x 20cm 20 x 41cm
Catalog No. FB-TIV-88A FB-TIV-816A
Each $1718.39 $1913.58
Additional FisherBiotech Ultraviolet Transilluminators are available. Please visit www.fishersci.com for details.
FisherBiotech Photodocumentation handheld Camera, shown with optional Tiffen® filters and FisherBiotech snap-on camera hoods.
FisherBiotech Photodocumentation Handheld Camera Obtain instant, permanent records of electrophoresis results. FisherBiotech Ultraviolet Transilluminators
Catalog No. FB-PDC-34
©2005 Fisher Scientific Litho in U.S.A.
Each $595.45
05-6809 BM/AO ???M-???-8/05
BN0816051
exACTGene, Fisher BioReagents, and FisherBiotech are registered trademarks of Fisher Scientific. AB . Hoefer is a registered trademark of Hoefer, Inc. Spectroline is a registered trademark of Spectronics Corp. Tiffen is a registered trademark of Tiffen Company, L.L.C.Triton is a registered trademark of Rohm and Haas. Tween is a registered trademark of ICI Americas. EasyCast is a trademark of Owl Separation Systems. PAGEgel is a trademark of PAGEgel, Inc. BlueRanger, Pierce, Precise, and TriChromRanger are a trademarks of Pierce Biotechnology. ReddyRun is a trademark of Advanced Biotechnologies Limited. Invitrogen is a trademark of Invitrogen.
