EUROPEAN CURRICULUM VITAE FORMAT

PERSONAL INFORMATION

Name Address (work) Telephone (work) Fax (work) E-mail Nationality Date of birth

LINDA MONACI VIA AMENDOLA 122/O - 70126 BARI (ITALY) +39 080 592.93.43 +39 080 592.93.74 [email protected] Italian 08-27-1971

WORK EXPERIENCE Dates Name and address of employer

Type of business or sector Occupation or position held Main activities and responsibilities

12/2008 to date National Research Council of Italy (NRC) Institute of Sciences of Food Production (ISPA) Via Amendola 122/O, Bari Food Safety and Quality Researcher Contribute to the EU project MoniQA “Monitoring and quality assurance in food supply chain”; member of the working group on food allergens. Develop LC-MS methods for the analysis of food allergens in incurred model food matrices like cookies. Fractionation and identification by LC-MS of peptides arising from milk proteolysis. Contribute to the harmonization of LC-MS methodologies for detection of food allergens within the analytical community. Develop LC-MS methods for detection of milk allergens markers in wine. Contribute to the EU funded project DREAM “Design and development of realistic food models with well characterized micro- and macro- structure and composition”. Contribute to produce in house bread food model. Investigate the effect of food processing on the stability/degradation of trichothecenes and identification of conjugation and/or degradation products by LC-high resolution MS. Study the fate of DON, T-2 and HT-2 during bread making and identification and characterisation of their conjugated products. Investigate trichothecenes bioaccessibility using an in vitro static digestion model. Assessment of the fate of AFM1 during storage of a dairy dessert model by HPLC-FL method. Cooperate to the inter-laboratory validation of analytical methods. Ensure international scientific networking in the field of food safety and quality. Disseminate scientific results to International conferences.

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Write reports and scientific publications. Dates Name and address of employer Type of business or sector Occupation or position held Main activities and responsibilities

Dates Name and address of employer Type of business or sector Occupation or position held Main activities and responsibilities

Dates Name and address of employer Type of business or sector Occupation or position held Main activities and responsibilities

Dates Name and address of employer Type of business or sector Occupation or position held Main activities and Page 2 - Curriculum vitae of [ SURNAME, other name(s) ]

11/2005 –11/2008 European Commission, Joint Research Center, Institute for Reference Materials and Measurements, Retieseweg 111 – B 2440 Geel (Belgium). Food Safety and Quality Scientific project officer • Develop LC-MS (ESI-Q-TOF and ESI-triple Q MS ) methods for the analysis of food allergens. • Contribute to the funded project: “Detection of peptides derived from milk hydrolysates by proteomic and immunochemical approaches”. • Perform database searching using bioinformatics tools. • Study the effect of food processing on milk allergens detection by mass spectrometric methods and ELISA methods. • Production of model food matrices. • Ensure the operation and maintenance of the scientific equipment and contribute with a sound scientific basis to the decision-making process. • Ensure international scientific networking in the field of food safety. • Disseminate the scientific results to International conferences. • Write scientific publications. 01/2005-10/2005 European Commission, Joint Research Center Institute for Reference Materials and Measurements, Retieseweg 111 – B 2440 Geel (Belgium). Food Safety and Quality Unit. Post-doctoral grant-holder • Design and carry out laboratory experiments. • Support the research program of the Unit. • Build-up a restricted allergen database. • Investigate on sample pre-treatment strategies for the extraction and purification of food allergens. • Develop chromatographic methods for the analysis of intact proteins in food products. • Support the planning and the organization of feasibility studies, homogeneity and stability studies. • Write internal reports and scientific publications. 04/2004 – 10/2004 Agrarian Faculty, University of Bari (Italy) Via Amendola 70126 Bari (Italy) Department of Agro-forestal and Environmental Biology and Chemistry Research contract • Study the interaction between phenolic acids and humic acids. • Optimization of chromatographic methods for the detection of phenolic acids in soil extracts. 06/2002-06/2004 Department of Chemistry – University of Bari. Via Orabona 4, 70125 Bari (Italy). Analytical chemistry Post-doctoral grant-holder • Develop HPLC/DAD UV and FLD methods for detection of mycotoxins in For more information go to www.cedefop.eu.int/transparency www.europa.eu.int/comm/education/index_en.html www.eurescv-search.com

responsibilities

food products. Develop HPLC-MS/MS methods based on ion trap MS for the detection of mycotoxins in food products. Perform in house validatin of the developed methods. Carry out a comparison between ELISA and HPLC/FLD method for detection of ochratoxin A in pig kidneys. Carry out a survey to monitor the contamination level of ochratoxin A in meet products collected in different European countries.

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EDUCATION 2001 Dates Name and type of organisation providing education and training Principal subjects/occupational skills covered Title of qualification awarded Dates Name and type of organisation providing education and training Principal subjects/occupational skills covered Title of qualification awarded Dates Name and type of organisation providing education and training Principal subjects/occupational skills covered Title of qualification awarded

PhD in Food Hygiene and Technology 11/1998-11/2001 Department of Chemistry, Analytical Chemistry group – University of Bari. Via Orabona 4, 70125 Bari (Italy). PhD student Subjects: analytical chemistry, mass spectrometry, food chemistry, biochemistry. PhD 1999-2000 Institute of Food Inspection, Department of animal health and wellness. Strada Provinciale per Casamassima km 3, 70010 Valenzano, BA (Italy). Faculty of Veterinarian Medicine, University of Bari (Italy).

Post-graduate specialization course in food control 11/1992-10/1998 University of Bari (Italy), Via Orabona 4, 70125 Bari (Italy). Experimental thesis in Analytical Chemistry.

University degree in Biological Sciences with mark 110/110.

PERSONAL SKILLS AND COMPETENCES MOTHER TONGUE

Other languages

ITALIAN

ENGLISH, FRENCH

Understanding

Self-assessment European level (*)

Listening

Speaking

Reading

Spoken interaction

Writing

Spoken production

English

Very good

Very good

Very good

Very good

Very good

French

Good

Good

Sufficient

Sufficient

Sufficient

FIELD OF EXPERTISE

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Proteomic-based methods for the study and analysis of allergenic proteins/peptides in food. Mass spectrometry (ion trap, triple quadrupoles, Q-TOF, Orbitrap

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based systems) Eletctrophoresis techniques (2DE) coupled to ms detection for the separation and identification of proteins in complex matrices. Chromatographic methods coupled to DAD-UV, FLD and MS/MS detection for the analysis of small contaminants (pesticides, mycotoxins) and peptides in foods. Study the fate of mycotoxins upon food processing. Investigate the stability of mycotoxins and its conjugates during digestion by in vitro digestion experiments. identification of degradation products by HPLC coupled to high resolution mass spectrometry. Develop biosensors and immunoassays applied to the field of food safety. Prefractionation and purification strategies combined to LC-MS analysis of peptides in food products

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ORGANISATIONAL SKILLS

INVITATIONS

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ADDITIONAL INFORMATION

Ability to communicate in public Planning and organizing capacities Coordination and networking skills Attitude to work in synergy and to lead small teams

Norwich (UK), 20-21 September 2010: she was invited to take part to the international workshop organized within the working group food allergens of the MoniQA consortium to draw dawn international guidelines for detection of food allergens by mass spectrometric methods. Prague (Czech Rep) 1-4 November 2011: invited seminar at the 5th edition of RAFA conference. Oral presentation “Feasibility of a Exactive Orbitrap system equipped with a high collision dissociation chamber for a reliable identification of food allergens”. San Diego (USA) 25-29 March 2012: invited lecture at the Symposium focused on Advances in food allergen detection organized by the American Chemical Society. Oral presentation: “New frontiers of mass spectrometry: high resolution MS applied to food allergen detection”.

REVIEWER OF THE FOLLOWING PEER REVIEWED INTERNATIONAL JOURNALS:

JOURNAL OF CHROMATOGRAPHY A, B JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY JOURNAL OF EUROPEAN FOOD RESEARCH AND TECHNOLOGY JOURNAL OF MAS SPECTROMETRY JOURNAL OF PROTEOMICS

TALANTA TOXINS FOOD CONTROL

MEMBER OF THE EDITORIAL BOARD OF THE JOURNAL ISRN CHROMATOGRAPHY

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TECHNICAL SKILLS AND COMPETENCES

ANAYTICAL TECHNIQUES: HPLC-DAD UV HPLC-FLD HPLC-ESI/MS MS INSTRUMENTS: Q-TOF MS, ION TRAP MS, ORBITRAP BASED MS, TRIPLE QUADRUPOLE MS. GC-ECD GC-MS TLC-UV CE-UV AMPEROMETRIC BIOSENSORS FT-IR SPECTROSCOPY PROTEOMICS TECHNIQUES

SDS PAGE

2D ELECTROPHORESIS MICRO-HPLC-ESI-QTOF-MS BIOINFORMATIC TOOLS SAMPLE PREPARATION TECHNIQUES

:

PEPTIDE FRACTIONATION AND PURIFICATION SOLID PHASE EXTRACTION SOLID PHASE MICROEXTRACTION IMMUNOAFFINITY COLUMNS ULTRAFILTRATION MAGNETIC BEADS MYCOTOXINS EXTRACTION AND PURIFICATION PROTEIN EXTRACTION AND FRACTIONATION PEPTIDE ENRICHMENT IMMUNOASSAYS: ELISA LATERAL FLOW DEVICES

FUNDED

PROJECTS

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2001. Coordinator of a project for young researchers funded by University of Bari “Development of analytical methods based on solid phase microextraction for the determination of Penicilium mycotoxins in food products”. 2006-2007 Coordinator of the Exploratory Research Project funded by the European Commission “Detection of peptides derived from milk hydrolysates by proteomic and immunochemical approaches”. 2007-2008 Coordinator of the Exploratory Research Project funded by the European Commission “Detection of potentially allergenic peptides from bovine milk enzymatic hydrolysates in food matrices by a proteomic approach”. 2008-2012 Participation to the network of excellence “Monitoring and quality assurance of food” funded by EC. She is member of the working group focused on actively involved in the standardization and harmonization of MS methods for the detection of allergens in food. 2009-2012 Participation to the large collaborative project DREAM “Design and development of realistic food models with well charcaterised micro- and macro- structure and composition” funded by EC. She leads the task on the assessment of the fate of mycotoxins during processing of 3 experimental food models (for trichothecens, patulin and aflatoxin M1) and on the bioaccessibility study of trichothecenes in a cereal based model food by using an in vitro digestion model.

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LIST OF PUBLICATIONS

ABSTRACTS PRESENTED AT NATIONAL/INTERNATIONAL CONFERENCES: 36

BOOK CHAPTER: 1 INTERNATIONAL PUBLICATIONS: 30 Monaci L. (invited paper). Liquid Chromatography coupled to Mass Spectrometry as a reliable tool for monitoring food allergens contamination, New Food, in press (2012) Quintieri L., Caputo L., Monaci L., Deserio D., Morea M. and Baruzzi F. Antimicrobial efficacy of pepsin-digested bovine lactoferrin on spoilage bacteria contaminating traditional Mozzarella cheese. Food Microbiology, in press (2012) Monaci L., De Angelis, E., Visconti, A. Determination of deoxynivalenol, T-2 and HT-2 toxins in a bread model food by liquid chromatography - high resolution - Orbitrap - Mass Spectrometry equipped with a high-energy collision dissociation cell Journal of Chromatography A, 2011, 1218, 8646-8654. Monaci L., Losito, I., Palmisano F., Godula M., Visconti, A. (invited paper) Towards the quantification of residual milk allergens in caseinate-fined white wines using HPLC coupled with single-stage Orbitrap mass spectrometry Food Additives and Contaminants, 2011, 28, 1304-1314. Monaci L., Losito, I., Palmisano, F., Visconti, A. (invited paper) Reliable detection of milk allergens in food using a highresolution, stand-alone mass spectrometer Journal of AOAC International, 2011, 94, 1034-1042. Johnson, P., Baumgartner, S., Aldick, T., Bessant, C., Giosafatto, V., Heick, J., Mamone, G., O’Connor, G., Poms, R., Popping, B., Reuter A., Ulberth, F., Watson, A., Monaci, L., Mills, E.N.C. Current perspectives and recommendations for the development of mass spectrometry methods for the determination of allergens in foods Journal of AOAC International, 2011, 94, 1026-1033. Monaci L., Brohée, M., Tregoat, V., van Hengel, A. Influence of baking time and matrix effects on the detection of milk allergens in cookie model food system by ELISA. Food Chemistry, 2011, 127, 669-675. Monaci L. and Visconti A. Immunochemical and DNA-based methods in food allergen analysis and quality assurance perspectives. Trends in Food Science and Technology, 2010, 21, 272-283. Monaci L., Losito I., Palmisano F. and Visconti A. Identification of allergenic milk proteins markers by capillary liquid chromatography-electrospray ion mass spectrometry, Journal of Chromatography A, 2010, 1217, 4300-4305.

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Monaci L., Nørgaard J.V., van Hengel A.J. Feasibility of a capillary LC/ESI-Q-TOF MS method for the detection of milk allergens in an incurred model food matrix. Analytical Methods, 2010, 2, 967-972. Monaci L. and A. Visconti, Mass spectrometry-based proteomics methods for analysis of food allergens, Trends in Analytical Chemistry, 2009, 28, 581-591. Monaci L. and van Hengel, Development of a method for the quantification of whey allergen traces in mixed-fruit juices based on liquid chromatography with mass spectrometric detection, Journal of Chromatography A, 2008, 1192, 113-116. Losito I., Carbonara T., Monaci L., Palmisano F. Evaluation of the thermal history of bovine milk for the lactosylation of whey proteins: an investigation by liquid chromatography- electrospray ionization mass spectrometry Analytical and Bioanalytical Chemistry, 2007, 389, 2065-2074. Monaci L. and van Hengel A. Effect of heat treatment on the detection of intact bovine β-lactoglobulins by LC mass spectrometry, Journal of Agricultural and Food Chemistry, 2007, 55, 2985-2992. Monaci L., Vatinno R., De Benedetto G. Fast detection of cyclopiazonic acid in cheese using Fourier transform mid- infrared ATR spectroscopy Journal of European Food Research Technology, 2007, 225, 585-588. Monaci L., Tregoat V., van Hengel A., E. Anklam. Milk allergens, their characteristics and their detection in food: a review Journal of European Food Research Technology, 2006, 223, 149-179. Matrella R., Monaci L., Milillo C., Palmisano F., Tantillo MT, Ochratoxin A determination in paired kidneys and muscle samples from swines slaughtered in southern Italy Food Control, 2006, 17, 114-117. Loffredo E., Monaci L., Senesi N. Humic substances can modulate the allelopathic potential of caffeic, ferulic and salicylic acids for seedling of lettuce (Lactuca sativa L.) and tomato (Lycopersicon esculentum Mill.) Journal of Agricultural and Food Chemistry, 2005, 53, 9424-9430. Monaci L., Palmisano F., Matrella R., Tantillo G. determination of ochratoxin A at part-per-trillion level in Italian salami by immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection Journal of Chromatography A, 2005, 1090, 184-187. Losito I., Monaci L., Palmisano F., Tantillo G. Determination of ochratoxin A in meat products by high-performance liquid chromatography coupled to electrospray ionization sequential mass spectrometry Rapid Communications in Mass Spectrometry, 2004, 18, 1965-1971.

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Monaci L., Tantillo MG., Palmisano F. Determination of ochratoxin A in pig tissues by liquid liquid extraction and clean-up and high performance liquid chromatography Analytical and Bioanalytical Chemistry, 2004, 378, 1777-1782. Aresta A., Monaci L. and Zambonin C. G. Determination of delorazepam in urine by solid phase microextraction coupled to high performance liquid chromatography J. of Pharmaceutical and Biomedical Analysis, 28, 2002, 965-972 Losito I., Monaci L., Aresta A., Zambonin C.G. LC-ion trap electrospray MS-MS for the determination of cyclopiazonic acid in milk samples The Analyst, 2002, 127, 499-502. Monaci L. and Palmisano F. Determination of ochratoxin A in foods: state-of-the-art and analytical challenges Analytical and Bioanalytical Chemistry, 2004, 378, 96-103. Monaci L., Aresta A., Palmisano F., Visconti A. and Zambonin C.G. Amino-bonded silica as stationary phase for liquid chromatographic determination of cyclopiazonic in fungal extracts, Journal of Chromatography A, 955, 2002, 79-86. Minervini F., Monaci L., Montagna MT, Dragoni I. Assessment of mycotoxin production by Aspergillus and Penicillium fungi isolated from dairy products Industrie Alimentari- XLI Dicembre, 2002. Guerrieri A., Monaci L., Quinto M., Palmisano F. A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts The Analyst, 2002, 127, 5-7. Zambonin C.G., Monaci L., Aresta A. Solid-phase microextractionhigh performance liquif chromatography and diode array detection for the determination of mycophenolic acid in cheese Food Chemistry, 78, 2002, 294-254. Zambonin C.G., Monaci L. and Aresta A. Determination of cyclopiazonic acid in cheese samples using solid-phase microextraction and high performance liquid chromatography Food Chemistry, 2001, 75, 2, 249-254. Minervini F, Montagna MT, Erroi R, Monaci L, Spilotros G. and Visconti A. Survey on mycoflora of cow and buffalo dairy products from Southern Italy Int. J. of Food Microbiology, 2001, 69, 1, 141146.

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