What is Seed Production Technology (SPT)?

0 What is Seed Production Technology (SPT)? A novel and proprietary seed production system • Significantly reduces production costs* (>20%) • Out-lic...
Author: Philomena Ellis
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What is Seed Production Technology (SPT)? A novel and proprietary seed production system • Significantly reduces production costs* (>20%) • Out-licensing opportunity • Improves product quality • Improves worker safety • Reduces environmental footprint

*SPT vs. detasseled acres in North America © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

1

Corn Seed Production Basics Step 1

Step 2

X

Hybrid seed

Inbred seed Inbred

Inbreds are produced by self-pollination

‘Male’ Inbred

‘Female’ Inbred

Hybrids are produced by cross-pollination of two different inbreds © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

2

Hybrid Seed Production Process Male Inbred Parent Production

Male Parent

Hybrid Seed (sold to customer)

± Female Inbred Parent Production

Female Parent (male sterile)

1X Acres

>50X Acres

Parent Seed Production (Selfing)

Hybrid Seed Production (Outcrossing)

Better yield Germplasm security Trait protection

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

3

Methods to Eliminate Selfing in Hybrid Seed Production

Manual (Detasseling)

Gametocide (Chemical Treatment)

Male-sterile females created at hybrid production stage

Genetics (Cytoplasmic/Nuclear)

Male-sterile females created at parent production stage © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

4

From Traditional Hybrid Seed Production…

Male Inbred

Female Inbred (Tassels Removed) Removed) © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

5

To Next Generation -- SPT

Any Male Inbred

SPT Female Inbred © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

6

Deploying SPT Mutation in a nuclear gene required for corn plants to make pollen

SPT Female

Method to produce pure inbred seeds from plants that do not make pollen © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

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The SPT Maintainer SPT female genetically engineered to be a pollen donor for producing pure inbred SPT lines

Allows SPT plants to produce pollen Imparts a color to SPT maintainer seeds Functionally contains all engineered SPT components in the maintainer line

SPT Female Non-transgenic

SPT Maintainer Transgenic © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

8

The SPT Maintainer SPT female genetically engineered to be a pollen donor for producing pure inbred SPT lines

Allows SPT plants to produce pollen Imparts a color to SPT maintainer seeds Functionally contains all engineered SPT components in the maintainer line

SPT Female SPT Female Non-transgenic

SPT Maintainer

SPT Maintainer Transgenic © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

9

SPT-based Female Seed Increase

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

10

SPT-based Female Seed Increase

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

11

SPT-based Female Seed Increase

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

12

SPT-based Female Seed Increase

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

13

SPT Maintainer Propagation

Self 50%

50% +

SPT Maintainer

SPT Maintainer

SPT Female

Transgenic

Transgenic

Non-transgenic

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

14

Producing the SPT Maintainer

Optical sorting

Optical sorting

Pure SPT Maintainer

(

)?

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

15

Traditional Hybrid Seed Production Male Inbred Parent Production

Male Parent Plant

Hybrid Seed (sold to customer)

± Female Inbred Parent Production

Female Parent Plant

Detasseling

Better yield Germplasm security Trait protection

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

16

SPT-based Hybrid Seed Production Male Inbred Parent Production

SPT Maintainer Production

Male Parent Plant

Hybrid Seed (sold to customer)

± Female Parent Plant

SPT Sterile Female Production

No detasseling needed

Better yield Germplasm security Trait protection

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

17

Seed Production Costs* Fraction of total costs

SPT impact

60%

15% - 30% productivity increase

Detasseling costs

10%

Eliminated

Other variable and fixed costs

30%

Field Management Costs Planting Pest Management Roguing Field Harvest Land, labor and inputs

Potential for injury

Intangible

Minimized

*SPT vs. detasseled acres in North America © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

18

Biotechnology for Production Field Performance Customer Benefits • Germplasm and trait purity Î Yield/Product Quality • Improved process efficiency Î Supply Reliability • No SPT transgenes in products Î No SPT yield drag

Additional Value Capture • Applicable to all germplasm - Can be leveraged across the industry • Differentiating technology

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

19

Current State Cutting

Pulling

Hand Detasseling © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

SPT Hybrid Production Field Utica, IL 2009

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Benefits of SPT • Works across all corn germplasm • >20% reduction in total production costs* • Maximum product/trait purity for customers • Safer production process for employees • ‘Greener’ technology; lower environmental footprint • No impact on hybrid yield for customers • Internally contained process – No SPT grain enters food/feed or grain channels

*SPT vs. detasseled acres in North America © 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

22

Trait Characterization and Development • Sr. Research Manager, Howard Hershey • Howard’s work focuses on applied agricultural biotechnology with special emphasis on the areas of plant reproductive biology and transgene trait development. • He joined DuPont as a research scientist in 1985 and has held positions in various departments ranging from Central Research and Development to Crop Protection to Agricultural Biotechnology. Howard moved to Pioneer in 2001 where he has held positions in the Agronomic Traits and Trait Characterization and Development Departments. • In his current role, Howard leads the SPT Implementation Office and also serves as Rice Trait Characterization and Development Liaison. • He holds a Bachelor of Science degree in Chemistry from Miami University and a Ph.D in Biochemistry from Michigan State University.

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

23

Global Product Strategy • Sr. Marketing Manager, Mat Müller • Mat's work focus is on developing competitive strategies to integrate our scientific and marketing efforts in discovery, development and launch of native and transgenic Agronomic Traits. • He joined Pioneer as a research scientist through our 2004 acquisition of Verdia, Inc. In 2005, he moved to the Midwest and to a new role as business development manager, and in 2008, was named senior marketing manager and science lead in the Global Product Strategy group. • In his current role, Mat has responsibilities on various product development and go to market teams, and frequently interfaces with our R&D, marketing and business development groups. • Mat holds a bachelor's degree in plant physiology and molecular biology and a Ph.D. in biochemistry from the University of Lausanne in Switzerland, and an MBA from the California State University at Hayward.

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.

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Pioneer North America • Johnston Parent Seed Plant Manager, Dick Hora • Started his career with Pioneer in 1981, as an Agronomist at the Marengo production plant. In 1985 he was promoted to Assistant Plant Manager. In 1998 he became Plant Manager for the Johnston Parent Seed location.



He is a graduate of Iowa State University with a Bachelor of Science degree in Agriculture Education with an emphasis in Agronomy. Prior to joining Pioneer, he worked on the family farm in Eastern Iowa.

© 2009 E.I. du Pont de Nemours and Company. All Rights Reserved.