Home Welcome to the 3rd Regional Conference on Biosensors, Biodiagnostics, Biochips and Biotechnology 2016 (3rdRC4Bs-2016). The conference is going to be organized by the AIMST University, Malaysia in collaboration with King Mongkut's University of Technology Thonburi, Thailand; Universiti Malaysia Perlis, Malaysia and Universiti Teknologi MARA, Malaysia. The conference will be held at the AIMST University, Kedah, Malaysia from 20–22 April 2016. The 3rdRC4Bs-2016 intends to provide a platform for scientists, researchers and students to share knowledge, challenges, recent advances and future perspectives in the multidisciplinary areas of biosensors, biodiagnostics, biochips and biotechnology. The 3rdRC4Bs-2016 welcomes academicians, researchers, students, entrepreneurs and industry players to present their works in a friendly and supportive environment. Both international and local speakers in the forefront of biosensors, biodiagnostics, biochips and biotechnology will actively engage in this 3-day event. The theme of the conference is “State-of-the-Art Achievements and Challenges in Biosensors, Biodiagnostics, Biochips and Biotechnology”. The scope of the conference includes but not limited to the following:  Biosensors  Biodiagnostics  Biochips  Biotechnology  Applications of biosensors, biodiagnostics and biochips in biotech industry  Current challenges in biotech industry in using biosensors, biodiagnostics and biochips  Emerging fields in biosensors, biodiagnostics, biochips and biotechnological innovations

The 3rdRC4Bs-2016 is jointly organized by

The 3rdRC4Bs-2016 is supported by 

Malaysian Biotechnology Information Center (MABIC), Malaysia



ScientiaBio, Bangalore, India

THE 3rd REGIONAL CONFERENCE ON BIOSENSOR, BIODIAGNOSTICS, BIOCHIPS AND BIOTECHNOLOGY 2016

Who Should Attend? The 3rd Regional Conference on Biosensor, Biodiagnostics, Biochips and Biotechnology 2016 (3rdRC4Bs-2016) features several talks [a keynote address, 6 plenary talks and 27 invited talks] and networking events to provide data, analysis, perspectives, insights, and opportunities in the area of Biosensor, Biodiagnostics, Biochips and Biotechnology. This conference will also highlight the achievements and challenges in the area of biosensor, biodiagnostics, biochips and biotechnology. During this three-day conference, leading scientists, academicians, executives, investors, developers, analysts, and economists will participate in scientific discussions on diverse and timely regional (and international) industry topics pertaining to Biosensor, Biodiagnostics, Biochips and Biotechnology. Who                    

will benefit from attending the 3rdRC4Bs-2016 conference? Students Postdocs Scientists Academicians Researchers Analysts Research Officers Science Officers Consultants Developers Franchisors Investment Bankers Investors Managers Owners Operators Product and service providers in biotech industry Product and service providers in biosensor domain Product and service providers in biodiagnostic sector Product and service providers in biochips domain

Program The tentative program for the conference is as follows: Day 1: Wednesday, 20th April 2016 9.00 am - 5.00 pm - Preconference Workshops Workshop 1: ‘Drug-Protein Interaction and Personalized Medicine’. (It will be conducted by ScientiaBio, Bangalore, India) Workshop 2: ‘Basic Statistics using Microsoft Excel and SPSS’ & ‘Basic Concept of Effective Scientific Writing and Communications’. (It will be conducted by Snr. Assoc. Prof. Dr. K. Marimuthu & Snr. Assoc. Prof. Dr. P. Balakumar, AIMST University) 7.45 pm: Registration & Welcome Reception for Participants at Orientation Hall, AIMST University Day 2: Thursday, 21st April 2016 08.00 am – 5.30 pm: Opening Ceremony; Keynote Address: 1; Plenary Talks: 3; and Presenters’ talk (selected from submitted abstracts): 12 Day 3: Friday, 22nd April 2016 08.00 am – 5.00 pm: Keynote Address: 1; Plenary Talks: 3; paper presenter’s talk (selected from submitted abstracts): 15, Young Scientist Awards (Oral and Poster category) distribution, and Concluding Remarks /Vote of thanks and Closing. Note: 1. Key note address talk will be for 45 minutes. 2. Each plenary speaker will have 30 min. 3. Each Presenter (selected from submitted abstracts) will have 15 minutes. 4. At appropriate time, the finalized program will be made available on conference website, www.aimst.edu.my/3rdRC4Bs-2016

Conference Organizing Committee Sponsors Sponsors (Names and their logos) info will be added soon. Advisors Snr. Prof. Dr. M. Ravichandran Prof. Dr. Mohd. Baidi Bin Bahari Assoc. Prof. Dr. S. Kathiresan Chairman Dr. Subhash J. Bhore Co-chairman Dr. Matiullah Khan Secretary Ms. Kalaiselvee Rethinam Scientific Committee Prof. Dr. M. Ravichandran (AIMST University, Malaysia) Prof. Dato' Dr. Mohd Zaki Salleh (UiTM, Malaysia) Prof. Dr. Uda Hashim (UniMAP, Malaysia) Prof. Dr. Teh Lay Kek (UiTM, Malaysia) Dr. Werasak Surareungchai (KMUTT, Thailand) Dr. P. Balakumar (AIMST University, Malaysia) Dr. Subhash J Bhore (AIMST University, Malaysia) Dr. Lee Su Yin (AIMST University, Malaysia) Dr. K. Marimuthu (AIMST University, Malaysia) Dr. Benchaporn Lertanantawong (KMUTT, Thailand) Dr. S. Kathiresan (AIMST University, Malaysia) Dr. V. Ravichandran (AIMST University, Malaysia) Dr. Kazi Selim Anwar (AIMST University, Malaysia) Dr. Sivakumar Pendyala (AIMST University, Malaysia) Dr. Ramesh Kumaresan (AIMST University, Malaysia) Secretariat Dr. Annie Jeyachristy Dr. Heera Rajandas Treasurer Mr. Vijayan Krishnan Logistics Committee Ms. Musalinah Buzri (website) Mr. V. Krishnan (Apartment & guest house accommodation) Ms. Yoganandhini (Hostel accommodation) Mr. Mahes D.S. (SGFS) (Event planning) Mr. Christapher V. (FOP) (Event planning) Mr. G. Prabhakaran (Food and beverages supply arrangements) Dr. Jawahar (Food and beverages supply arrangements) Publicity & Exhibitions Committee Dr. Sivachandran P. (Coordinator) Mr. Siventhiran B. (RMC) Mr. M. K. Faiz (Marketing) Mr. K. Dhanaraj (CLMU) Mr. P. K. Karuna (CLL)

Reception Committee Dr. Sridevi Viswanathan Ms. Elil Suthamathi Dr. Tahmina Monowar Ms. Azdlina First Aid and Safety Committee Dr. Sawri Rajan Dr. Leela A. J. Mr. Maheswaran (FAS)

Keynote Address and Plenary/Invited Talks Keynote Speaker

Keynote Address Talk Title

Moving the Regional Biotechnology and Bioeconomy Forward Dato’ Prof. Dr. Asma Ismail Director General Department of Higher Education, Ministry of Education, Malaysia

Nanotechnology Oriented Biosensors and Biomedical Applications

Prof. Eiichi Tamiya Osaka University, Japan Plenary Speaker

Plenary Talk Title

Supercomputing Makes Big Data Widely Applicable in the Biotechnology Industry Assoc. Prof. Dr. Bent Petersen Department of Systems Biology, Technical University of Denmark, Denmark

Highly Sensitive Detection of DNA Hybridization and Immunoassay Based on Nanomaterials Assoc. Prof. Dr. Werasak S. KMUTT, Thailand

Current Progress in Cholera Diagnostics Snr. Prof. M. Ravichandran AIMST University, Malaysia

Bio-Applications of Innovative Nano-materials Dr. T.Theivasanthi, International Research Center, Kalasalingam University, India

Relevance of Biotechnological Applications for Global Food Security and Sustainability Prof. Dr. Prakash Kumar NUS, Singapore

Assoc. Prof. Dr. Phua K.K. USM, Malaysia

Line Probe Assay for Simultaneous Detection of Multiple Salmonella Pathogens Causing Typhoidal and NonTyphoidal Salmonellosis in Humans

Nanotechnology in Medical Diagnostic Applications Prof. Dr. Uda H. UniMAP, Malaysia

Presenters’ Talk Titles Speaker’s names and titles of their talk will be announced after screening of the submitted abstracts.

Registration Fees

Participant

Academics & Scientists Student* Company Representatives Accompanying person**

Early bird registration (before February 29, 2016) Local International (RM) (US$) 600 200

Standard registration (after February 29, 2016) Local International (RM) (US$) 700 250

300 800

100 350

400 900

150 400

100

50

150

100

Spot Registration Local International (RM) (US$) 1000 500 Registration fees is including GST 15% discount for group registration (more than 3 participants from same laboratory) Registration fees cover conference materials, lunches, refreshments and a banquet dinner. * Participants under the category of student registration are needed to submit an official letter of studentship from the respective university or a scanned copy of a student identity card and participation approval signature from the head of the department or academic advisor/ main supervisor. All documents/letters must be in English. ** Accompanying person is entitled to have lunches, refreshments and a banquet dinner.

Registration Payment Guidelines Please make the payment early to take advantage of the early-bird concessions. The international and local participants can pay their registration fee by following a suitable payment mode as stated below: For international Participants i. ii.

Banker’s Draft Telegraphic Transfer

For Local Participants iii. iv. v.

Banker’s Cheque Cash Direct Debit

For the registration fees payment, please follow the details as shown below: Beneficiary Name : AIMST SDN. BHD. A/C 2 Bank Name

: CIMB Bank Berhad

Bank Branch

: Wisma Ria, Sungai Petani

Bank Address

: CIMB Bank Berhad Wisma Ria, Taman Ria, 08000 Sungai Petani, Kedah Darul Aman. Malaysia

Account Number : 8006376336 SWIFT Code

: CIBBMYKL

Please scan the inter-bank transfer receipt and email to [email protected] and [email protected] OR fax to +604-429 8009 (on fax write: Attention to 3rdRC4Bs-2016 Secretariat) Registration and Payment Instructions 1. Download the registration form. 2. Fill and submit the registration form to [email protected] and [email protected] 3. Participating students are required to attach a letter from the main supervisor stating permission to present his/her research findings and availability of financial support or studentship.

4. Please scan/photograph/screenshot a copy of your online payment details/Bank Slip/Guarantee Letter and email it to the Conference Secretariat at [email protected] and [email protected] OR fax to +604429 8009 as a proof of registration payment. 5. Upon receiving the proof of payment, confirmation of payment and registration will be sent to the e-mail address of the respective delegate. 6. Official receipts of the payment will be provided on the day of conference registration. Important Note 1. The organizer should receive the full fee excluding bank charges imposed by bankers and bank charges need to be borne by the participants or participating institutions/organizations. 2. We will update the payment status upon receiving the proof of transaction. 3. No refund of fees will be granted in the event of registration cancellation or withdrawal.

Call for Papers and Abstract Submission Authors are invited to submit original papers on topics related to the conference scope. All submissions must be on original research that is not published or currently under review by any another conference or journal. All accepted abstracts and extended abstracts will be published in the ‘Conference Abstract Book’ and ‘Conference e-Abstract Book’ with an ISBN and eISBN code, respectively. Selected papers will be published in a special issue of a peer-reviewed journal, Malaysian Journal of Microbiology (MJM). MJM is indexed in SCOPUS, CAS, CABI, WPRIM, DOAJ, MyCite, MyAIS, Emerging Sources Citation Index (ESCI) and Google Scholar [Note: It is likely that MJM will be indexed as an ISI journal in 2016]. To know more about this journal, visit http://mjm.usm.my/ The scope of the conference includes but not limited to the following:  Biosensors  Biodiagnostics  Biochips  Biotechnology  Applications of biosensors, biodiagnostics and biochips in biotech industry  Current challenges in biotech industry in using biosensors, biodiagnostics and biochips  Emerging fields in biosensors, biodiagnostics, biochips and biotechnological innovations Abstract Submission Abstracts must be prepared by following the 3rdRC4Bs-2016 guidelines to prepare the abstract. Abstract and completed registration form submissions must be emailed to [email protected] and [email protected] on or before January 31, 2016. We welcome abstracts within the scope of the conference. Submitted abstracts will be reviewed by the scientific committee. The submitted abstract that are not in the format of the guidelines shall be returned back to the author for timely revision while irrelevant abstracts shall not be considered for presentation. Please submit your abstract(s) along with registration form to: [email protected] and [email protected] Guidelines to Prepare the Abstract i.

Abstract should be written in English.

ii.

The title should not be more than 15 words.

iii.

Abstract should not be more that 250 words, excluding title, author names and affiliations.

iv.

It is necessary to state 4-6 keywords following the abstract.

v.

Abstract must include 4 major sections such as Background, Methods, Results, and Conclusions.

vi.

The Organizing Committee reserves the rights to edit the abstract text to maintain the quality and or consistency.

vii.

The abstract should be original and not be submitted previously to any other conference/meeting.

viii. The

presenting author’s name must be underlined, and addition of new author after the submission and or acceptance of the abstract is not encouraged.

ix.

The presenting author must register to the conference. The abstract of the unregistered candidates will not be processed for publication in the proceedings.

x.

The presenting author will be responsible for the accuracy of scientific contents of the submitted abstract. The organizing committee is not responsible for the results and conclusion provided in the abstract.

Please refer to following format OR download a template here ------------------------------------------------------------------------------------------------------------

Title of the Abstract (Title Case) Presenting Author (must be underlined), Coauthor A., Coauthor B. and Coauthor C* Department name, Faculty name (if applicable), University/Institute name, place, State, Country; *corresponding author, E-mail: email address; Ph No: (country code) phone number Abstract: Background: Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Methods: Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Results: Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Conclusions: Discuss here the concluding remarks only on the basis of results obtained. Discuss here the concluding

remarks only on the basis of results obtained. Discuss here the concluding remarks only on the basis of results obtained. Keywords: 4-6 key words separated by using semicolon.

Note: 1. Author name format: [Family name] [Given Name Initial] [Father’s Name Initial]. For instance, author name ‘Bhore Subhash Janardhan’ should be written as ‘Bhore S. J.’ 2. Please do not change Font, Font Size, page margins, and other format of this template. -----------------------------------------------------------------------------------------------------------Corresponding authors of all accepted abstracts will be notified via email on or before February 15, 2016. The type of presentation, i.e. oral or poster will also be notified and the final decision will be made by the Scientific Committee of the Conference.

Guidelines for Presenters Oral Presentations (invited speakers): 1. The presenting author will be provided 10 min for PPT oral presentation, followed by question and answer session for 5 min. Presentations of the young scientists will be judged by a panel of judges. 2. The presenting author is requested to upload the PPT presentation in the allotted seminar hall 30 min before the schedule. 3. Power point projector and pointer will be made available for the presentation. 4. An organizing committee member shall be available for timely help. Poster Presentations: 1. A panel area of about 1m x 1.5m will be available for each poster presenter. 2. All presenters are required to mount their posters on April 21, 2016 morning and remove them at the end of the last session on April 22, 2016. 3. Poster presenters should be present during poster viewing sessions for an interactive question and answer session with conference participants and judges. Below we provide the model copy of the poster design and dimensions.

Best Presenter Award [Young Scientist Award] Best Oral/Poster Presenter Award The best presenter awards [Young Scientist Awards] will be given by the Organizing Committee to recognize the contribution of young delegates. The guidelines are as follows: 1. Age limit below 35 years on the date of presentation. 2. The presenting author has to register for the Conference. 3. The presenting author must be the first author. 4. Full paper formatted in Malaysian Journal of Microbiology (MJM) style [Refer to Instructions for Full Paper Submission under ‘Download’ hyperlink] must be sent to the secretariat at the email [email protected] and [email protected] on or before March 18, 2016. 5. The Organizing Committee will establish a panel of 4 members to review and recommend maximum 5 papers form oral presentation category and 5 papers from poster presentation category. The authors of the nominated papers shall be informed before March 30, 2016. 6. The Scientific Committee shall appoint a panel of 3 judges who will evaluate (nominated oral and poster) presentations and recommend a candidate for the Best Oral and Best Poster Presenter Award along with first and second runnersups.

Venue of the Conference

The venue of the conference is in the AIMST University campus. The university campus is nestled on a 230-acres site within the foothills bordering the picturesque Gunung Jerai Mountain in Kedah Darul Aman, Malaysia. Archaeological evidence suggests that Kedah is the site of establishment of Peninsular Malaysia’s oldest civilization. The Bujang Valley civilization, which began in the tenth century AD attracted a cosmopolitan population of merchants and traders, including Indians, Chinese, Achenese, Burmese and Arabs. The arrival of Arab traders signaled the first advent of Islam into the Peninsula. The archeological findings reveal that the State was once a prosperous center for trade and a focal point for knowledge and learning. The establishment of the AIMST University in this historical site is indeed significant. The self-contained university campus has a circular design that alludes to the ever-spinning wheel of progress; a ubiquitous symbol which transcends many cultures and traditions. Campus is ultra clean and free from sound (noise), water and air pollution. Several tourist places popular in Malaysia are located near to the AIMST University. To visit the AIMST University website click here.

A road map to reach to the AIMST University

To view AIMST University’s google map click here

Accommodation Hotel Accommodation There are many Hotels near to the AIMST University. Participants can stay in any suitable Hotels listed below: Park Avenue Sungai Petani (near Sungai Petani South Toll) E-1 Jalan Indah Dua, Taman Sejati Indah, 08000 Sungai Petani, Kedah Darul Aman. Tel : +604-4317777 Fax : +604-4318777 E-Mail : [email protected] / [email protected] Website : www.parkavenue.com.my Emerald Puteri Hotel (near Sungai Petani South Toll) 50 Jalan Permatang Gedong, Taman Sejati Indah, 08000 Sungai Petani, Kedah Darul Aman. Tel : +604-4315555 Fax : +604-4311555 Email: [email protected] Website: www.emeraldputerihotel.com Hotel Seri Malaysia (Sungai Petani Town Center) Seksyen 21, Jalan Pasar, 08000 Sungai Petani, Kedah Darul Aman. Tel : +604-4234060 Fax : +604-4234106 E-Mail : [email protected] / [email protected] Website : www.serimalaysia.com.my Cinta Sayang Golf & Country Resort (Sungai Petani North Toll) Persiaran Cinta Sayang, 08000 Sungai Petani, Kedah Darul Aman Tel : +604-4414666 Fax : +604-4415600 E-Mail : [email protected] Website : www.cinta-sayang.com.my / www.cintasayangresort.com Swiss Inn (near Sungai Petani North Toll) No 1, Jalan Pahlawan, 1

08000 Sungai Petani, Kedah Darul Aman Tel : +604-4223333 Fax : +604-4233423 E-Mail : [email protected] / Website : www.swissgarden.com

[email protected]

Casa Lagenda Hotel (near Sungai Petani North Toll) 15, Jalan Pahlawan, Jalan Lagenda 2, Lagenda Height, 08000 Sungai Petani, Kedah Darul Aman Tel : +604-4258972/+604-4248972 Fax : +604-4227972 Website : www.casalagenda.com 3G Hotel (near Sungai Petani North Toll) No.3,Taman Lagenda Indah, 08000 Sungai Petani, Kedah Darul Aman Tel : +604-4212344 Fax : +604-4212366 E-Mail : [email protected] Website : www.three3g.spaces.live.com Harvard Suasana Hotel (near Sungai Petani North Toll) Harvard Golf and Country Club 08100 Bedong, Kedah Darul Aman Tel : +604-458 8888 Fax : +604-458 5888 Website : www.harvardhotel.com.my Sapphire Hotel (near Sungai Petani North Toll) 2A-D,4A-D Lorong Permaipura Damai, Taman Permaipura, 08100 Bedong, Kedah Darul Aman Tel : +604-4595383 Participants interested to stay in the AIMST University campus can reserve the rooms/apartments in advance to avoid the disappointment. University-Campus Accommodation In campus we have Hostels, Apartments and Guest house. Participants can choose triple or twin sharing 2

Guest House: RM 80/day (for locals); US$ 50/day (for international) Guest Apartment: RM 40/room/day (for locals); US$ 30/room/day (for international) Hostel (Students residence): RM 35/day (for locals); US$ 22/day (for international) Note: Limited seats are available in AIMST University’s campus; hence, reserve your seat in advance to avoid disappointment.

3

Important Dates and Deadlines  Submission

of the abstract

 Notification

of the accepted abstract

 Early

bird registration deadline

 Submission

January 31, 2016 February 15, 2016 February 29, 2016

of the extended abstract / full March 18, 2016

paper  Conference

registration deadline

 Conference

date

March 21, 2016 April 20-22, 2016

Title of the Abstract (Title Case) Presenting Author (must be underlined), Coauthor A., Coauthor B. and Coauthor C* Department name, Faculty name (if applicable), University/Institute name, place, State, Country; *corresponding author, E-mail: email address; Ph No: (country code) phone number Abstract: Background: Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Discuss here the background and objective of the research work. Methods: Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Discuss here the methods and parameters employed in the research work. Results: Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Discuss here the key outcomes of the research work. Conclusions: Discuss here the concluding remarks only on the basis of results obtained. Discuss here the concluding remarks only on the basis of results obtained. Discuss here the concluding remarks only on the basis of results obtained. Keywords: 4-6 key words separated by using semicolon.

Note: 1. Author name format: [Family name] [Given Name Initial] [Father Name Initial]. For instance, author name ‘Bhore Subhash Janardhan’ should be written as ‘Bhore S. J.’ 2. Please do not change Font, Font Size, page margins, and other format of this template.

1

Manuscript Preparation Instructions Full papers will be published in a special issue of ‘Malaysian Journal of Microbiology. Manuscript Preparation Instructions are given below: Types of article Research Articles: This regular or full paper should be written in the style described in “Instruction to Authors”. It should contain new and significant information as well as sufficient details to enable others to repeat the work. Short Notes: These papers may be short research reports which contain material of unusual interest but which are not sufficient to form the basis of a regular paper. Such communications must not exceed four printed pages (10 double-spaced typewritten pages) including figure(s), table(s) and references. Each note must have a short abstract of no more than 50 words. Do not use section headings in the body of the text. Review Articles: These papers will be published upon invitation only and should not exceed 25 pages of printed text, including tables, figures and references. Title Page The first page of the manuscript should include the following information: -The title of the paper, which should be concise. - A maximum of 5 keywords. - The names of the author (Please provide the full name of all authors and underline the surname) - Present addresses of authors. - Complete name, address, telephone, fax numbers and an e-mail address of all the corresponding and co- authors.

Abstract Should be written in a structured format (between 150-250 words) presenting concisely the (i) aims, (ii) methodology and results and (iii) conclusion, significance and impact of study. For example: Aims: Prodigiosin is a bright red pigment produced by certain strains of Serratia marcescens, characterized by a common pyrrolylpyrromethane skeleton. This pigment is found to possess antibacterial, antifungal, immunosuppressive and antiproliferative activity. The present study aimed at designing process parameters for the enhanced production of this pigment. Methodology and results: Peptone glycerol broth was selected as the best synthetic medium. The effects of various media components and process parameters like carbon and nitrogen sources, temperature, pH, incubation period and other supplements were investigated. Maximal amount of prodigiosin was produced at temperature 25 °C, pH 7.0 and incubation period of 48 h. Supplementation of media with maltose and peptone 2

yielded maximal amount of prodigiosin. Incorporation of minimal amount of supplements like silica gel, iron salts, inorganic phosphate also showed promising results. Chromatographic separations suggested that prodigiosin is made up of three different fractions (purple, orange and red). Further investigation of antimicrobial properties of prodigiosin revealed that it is a potent inhibitor against gram positive bacteria like Staphylococcus aureus and Bacillus cereus and fungal pathogens like Candida albicans, C. parapsilosis and Cryptococcus sp. This antimicrobial potency remained stable under a wide range of temperature and pH. The antioxidant capacity of prodigiosin was found to be 22.05 Bg ascorbic acid equivalents/mL of extract. When applied to textiles, prodigiosin resisted the action of acid, alkali and detergent. Conclusion, significance and impact of study: Besides combating gram positive bacterial pathogens and some pathogenic yeasts, prodigiosin with strong dyeing and antioxidant activity may find broad applications in textile and therapeutic industries. Abstract is adapted from Gulani et al., 2012. Text Neatly typed on one side of an A4 size paper, double-spaced throughout (all parts including title, address, captions, tables and references) and with wide margin (2.5 cm on all 4 sides). Use line numbers for review copies, and remove them for final copy. Primary headings (all in upper case and bold) are INTRODUCTION, MATERIALS AND METHODS, RESULTS AND/OR DISCUSSION, REFERENCES and ACKNOWLEDGEMENTS (if any). Sub-heading can be used when necessary and written in lower case and bold. Footnotes should not be used. 1. INTRODUCTION Introduction should provide brief background of the work in the relevant field and the reason why it is worth studying as well as the objectives of the work. 2. MATERIALS AND METHODS This section should include sufficient details of technical information to allow the work to be repeated. The source of uncommon chemicals and research materials should be given. 3. RESULTS AND DISCUSSION The results and discussion section can be separated. Sub-heading can be used where appropriate to organise the results. Discussion should provide an interpretation of the results and its relationship with other relevant published work. 4. REFERENCES List at the end of the paper and placed in alphabetical order. Some examples are given as follows: Journal article 3

Saha, B. C. and Zeikus, J. G. (1989). Improved method for preparing high maltose conversion strups.Biotechnology and Bioengineering 43, 23-25. Book Agrios, G. N. (1988). Plant pathology. Academic Press. New York. pp. 15-20. Chapter in a book Bailey, J. A., O’Connell, R. J. and Nash, C. (1992). Infection strategies of Colletotrichum species. In: Colletotrichum – Biology, Pathology and Control. Bailey, J. A. and Jeger, M. J. (eds.). CAB International, Wallingford, England. pp. 88-120. Thesis Uronu, A. B. (1989). Studies on the infection of pea (Pisum sativum) by Colletotrichum pisi. M.Sc. Thesis. University of Bristol, U. K. Conference Proceeding Papers Green, P. N. Wood, D. C. and Dow, C. F. (2002). Taxonomic status of some methogens. In: Crawford A. D. and Lind, B. C. (eds.). Proceedings of the 4th International Symposium. American Society for Microbiology, Washington, D.C. U.S.A. pp.141-145. Internet references are NOT encouraged. Units and Abbreviations Use only SI units of measurement, (e.g. cm, kg, g, mL, L, sec, min, h) and other common units (e.g. bp, kb and Da). The use of abbreviation should be avoided except for those universally accepted. Define each abbreviation and introduce it in parentheses the first time it is used. A few examples are as follows: DNA (deoxyribonucleic acid), cDNA (complementary DNA), NAD (nicotinamide adenine dinucleotide), NADP (nicotinamide adenine dinucleotide phosphate), PFU (plaqueforming units), CFU (colonyforming units), PCR (polymerase chain reaction), ELISA (enzyme-linked immunosorbent assay), SDS (sodium dodecyl sulphate). Nomenclature of Microorganisms Microorganisms must be correctly named following the current codes for bacteria, yeasts, fungi, algae and protozoa.

Figures Submit relevant figures only. Figures and photographs should be labeled as Figure and submitted on a separate page. Figures should be numbered according to their 4

sequence in the text. For digitally generated photographs, the resolution should be at least 300 d.p.i. Photographs should be supplied in black and white and have good contrast. Colour figures are welcomed for online publication, however, the hardcopy publication will be printed in black and white.

Tables Each table should be typed on a separate page. Use Arabic numerals, e.g. Table 1 and numbered consecutively. Tables should be constructed based on the formats given below: Type 1: Operating System AAA BBB CCC

Value 1234 1234 1234

Type 2: Specimen details

Total number 50 100

A B

Batch No. A B C

Reaction Positive Negative 25 25 25 75

1

B

C

Xxx Xxx Xxx

Xxx Xxx Xxx

Xxx Xxx Xxx

Percentage of positivity 50 % 25 %

R Value (log B / C) 1 1 2

Antimicrobial Efficacy (%) 99 99 100

Type 3:

Type 4: Wood samples AAA

BBB

Solvents Aa Bb Cc Aa Bb Cc

1 + + + + + +

2 + + + + + +

3 + + + + + +

4 + + + + + +

Metabolites 5 6 + + + + + + + + + + +

7 + + + + + +

8 + + + + + +

9 + + +

5

Checklist for Technical Corrections and Format Checklist Yes (Y) / No (N) 1. Author’s name: must be in full and surnames are to be underlined 2. Abstract: should comply to MJM format Aims, Methodology & Results, Conclusion, Significance and Impact of study 3. Keywords should be relevant 4. E-mail addresses of all co-authors should be provided 5. References: should comply to MJM format 6. All tables and figures should follow MJM format. The total number of both tables and figures should not exceed 8. All line bar/graph should not be colored, and use patterns instead 7. Authors should suggest 2 potential reviewers. reviewers should be from another country -

The

Complete name, address, telephone, fax numbers and an e-mail address of the corresponding author and co-authors.

6

December 30, 2015 Dear Sir/Madam, SUBJECT: AN INVITATION TO BECOME A SPONSOR On behalf of the organizing committee, we are pleased to inform you that the 3rd Regional Conference on Biosensors, Biodiagnostics, Biochips and Biotechnology 2016 (3rdRC4Bs-2016) will be held at AIMST University, Kedah, from April 20-22, 2016. This conference is the first of its kind in the country and it is a “must-attend” event for those in the field of Biosensors, Biodiagnostics, Biochips and Biotechnology, for both attendees and exhibitors alike. The 3rdRC4Bs-2016 intends to provide a platform for scientists, researchers and students to share their research findings, knowledge, challenges, recent advances and future perspectives in the areas of Biosensors, Biodiagnostics, Biochips and Biotechnology. The 3rdRC4Bs-2016 theme is “State-of-the-Art Achievements and Challenges in Biosensors, Biodiagnostics, Biochips and Biotechnology”. This theme is selected to explore the myriad ways in which we pursue the goals of our research in those respective fields. To this end, the conference will present a wide range of invited speakers who are internationally renowned. This conference is expected to draw more than 250 participants including some eminent speakers from various parts of the Asia, who are actively doing research in the multidisciplinary fields of Biosensors, Biodiagnostics, Biochips and Biotechnology. The 3rdRC4Bs-2016 will serve as an ideal networking platform for scientists and companies. The varieties of offerings at the 3rdRC4Bs-2016 are tailored to meet the needs of the participants. The exhibit hall will provide attendees with the opportunity to learn about the latest products and services available for research in those 4 respective fields. We therefore cordially invite your company to participate and contribute to this conference as your side plays an integral role in research advancements in the field of Biosensors, Biodiagnostics, Biochips and Biotechnology. Please come and join us. Information about various sponsorship packages is appended with this letter for your quick reference. To become a ‘Sponsor’ and for more information, kindly contact the following members of the organizing committee or email us at [email protected] 1. Dr. Sivachandran P. (Phone No: 017 5817 018) 2. Mr. Sivendran B. (Phone No: 014 9446 815) 3. Dr. Subhash J Bhore (Phone No: 016 6420 868)

Positively, we are looking forward for your kind contribution and support to make this event a successful one. We will highly appreciate if you could kindly fill in the attached ‘Sponsorship Confirmation and Reply Form’ and send it to us to confirm your ‘Sponsorship’ at your earliest. Thank you! Sincerely,

−−−−−−−−−−−−−−−−−−−−−−−−−−−− Snr. Assoc. Prof. Dr. Subhash J. Bhore Chairman Organizing Committee of the 3rdRC4Bs-2016 Note: For more information about the conference agenda and programme please visit http://www.aimst.edu.my/3rdRC4Bs-2016 List of Attached Documents: 1. Sponsorship Confirmation and Reply Form 2. Sponsorship Categories and Entitlements 3. Additional Information for Sponsor 4. Conference Brochure

Regional Conference on Biosensors, Biodiagnostics, Biochips and Biotechnology 2016 April 20-22, 2016; AIMST University, Malaysia SPONSORSHIP CONFIRMATION AND REPLY FORM Company Name: Company Address:

Company Contact info: Tel. No.: Fax No. : Contact Person Name: Contact Person Contact Details: Tel.No. (H/P):

Tel. No. (O):

SPONSORSHIP CATEGORIES (Please tick (✓) the sponsorship package of your choice) Diamond sponsor (RM 5,000) Platinum sponsor (RM 3,500) Gold sponsor (RM 2,500) Shell scheme Booth (RM 2,000) Slide advertisement (RM 1,000) Advertisement in 'Conference Abstract Book' Full page: RM 2000/Half page: RM 1000/Monetary sponso*: Specify the amount (RM): ___________________ *Any amount of cash sponsorship from individuals, company or organization is welcome. Monetary sponsorship of more than RM 500 will be listed in the 'Conference Abstract Book'. Remarks (if any):

Note: Upon filling this form, kindly scan and e-mail it to [email protected] OR after completing this form, take its photo and send it to Dr. Sivachandran by What’s App to: 0168740045 OR 0175817018

________________________________ Signature & Company’s Stamp

SPONSORSHIP CATEGORIES AND ENTITLEMENTS 1. Diamond sponsor (RM 5,000) Complimentary single booth exhibit space, may be upgraded to a double booth for an additional RM 500. Complimentary commercial presentation slot for 15 minutes. Complimentary slide advertisement. Complimentary full page advertisement in 'Conference Abstract Book'. Complimentary lunch and refreshments for 2 persons. Recognition as a diamond sponsor with company logo and name displayed on all official conference publications (i.e. poster, website, and backdrop).

2. Platinum sponsor (RM 3,500) Complimentary single booth exhibit space, may be upgraded to a double booth for an additional RM 650. Complimentary slide advertisement. Complimentary half page advertisement in 'Conference Abstract Book'. Complimentary lunch and refreshments for 2 persons. Recognition as a platinum sponsor with company logo and name displayed on all official conference publications (i.e. poster, website, and backdrop).

3. Gold sponsor (RM 2,500) Complimentary slide advertisement.  Complimentary half page advertisement in 'Conference Abstract Book'. Recognition as a gold sponsor with company logo and name displayed on all official conference publications (i.e. poster, website, and backdrop).

4. Shell scheme booth (RM 2,000) Complimentary single booth exhibit space, may be upgraded to a double booth for an additional RM 650. Complimentary lunch and refreshments for 2 persons. Booth size: Single booth (3 m X 3 m); Double booth (3 m X 6 m).

5. Slide advertisement (RM 1,000) Complimentary slide advertisement during the conference.

6. Advertisement in 'Conference Abstract Book'  

Full page : RM 2000/Half page: RM 1000/-

7. Monetary sponsorship Any amount of cash sponsorship from individuals, company or organization is welcome. Monetary sponsorship of more than RM 500 will be listed in the 'Conference Abstract Book'.

Note: 'Conference Abstract Book' will be published both as ‘Print version’ and ‘e-Book version’ with ISBN and e-ISBN codes. E-Book will be available on internet forever. 1

ADDITIONAL INFORMATION FOR SPONSOR 1. Commercial presentation The speaking opportunity is strictly limited to the time allotted by the organizer which is 15 minutes. The sponsor will provide the organizer with the speaker’s name, business title and onsite contact information by April 11, 2016. The organizer will provide an introductory slide with the speaker’s name, title and company. The organizer will assign speaking slots on a first-come, first-served basis and may change the assigned times. The speaker is only allowed to use PowerPoint slide to complement his/her presentation. The presentation must be saved in PowerPoint 2007 or 2010 format, at a 16:9 aspect ratio, with all fonts embedded and should be received by the organizer no later than April 11, 2016. Use of audio or video must be reviewed in advance by the organizer audio-visual team (prior to April 15, 2016).

2. Slide advertisement Slides must be saved in PowerPoint 2007 or 2010 format, at a 16:9 aspect ratio, with all fonts embedded. Slides must be received by the organizer no later than April 15, 2016.

3. Booth specification and exhibitor guidelines The organizer will provide a 3 M X 3 M shell scheme booth (white) with needle punch carpet. In addition, the organizer will also provide the following:  1 table  2 folding chairs  1 rubbish bin  fluorescent light  plug point  fascia panel with your company name All equipment, signage and other materials must fit within the space of the booth size. Sound generated by exhibitor’s equipment should not be so loud as to impact other exhibitors and attendees. Exhibitors are required to give the names of their representatives (not more than 2) by April 15, 2016. Exhibitors are required to wear the conference tag throughout the conference period. Complimentary lunch and refreshments will be provided for 2 persons during the conference period.

2

STANDARD SHELL SCHEME BOOTH

4. Move-in, move-out, and storage Move-in: April 21, 2016 (Thursday) 8:00 – 5:00 pm Move-out: April 22, 2016 (Friday) 5:00 – 8:00 pm Immediately after the end of the event, exhibitor must remove all property/belongings from the exhibit area. Exhibitor is solely responsible for arranging their packing and material transportations. No storage will be provided. All storage materials must be out of sight during the conference.

5. Exhibit time Exhibits are open during the whole period of the conference starting from 8:00 am-5:30 pm. Exhibitors are strongly encouraged to be at their booth all the times.

6. Exhibit security The organizer will not be held responsible for any loss, damage, accident and theft that occur during the conference period. Hence, the organizer recommends removing valuables when not attended. Items left unattended are at exhibitor’s risk.

3

7. Internet access Internet access via Wi-Fi will be provided throughout the conference period by the organizer.

4

Guidelines to Prepare the Abstract

Registration & Fees Participant

Early bird registration

Standard registration (after February 29, 2016 )

(before February 29, 2016 ) Local (RM)

Internatio nal (US$)

Local (RM)

International (US$)

Academics & Scientists Student*

600

200

700

250

300

100

400

150

Company Representatives

800

350

900

400

Accompanying person

100

50

150

100

Spot Registration Local (RM)

International (US$)

1000

500

Registration Payment Guidelines Please make the payment early to take advantage of the early-bird concessions. The international and local participants can pay their registration fee by following a payment mode:

For international Participants Banker’s Draft/Telegraphic Transfer

For Local Participants

Banker’s Cheque/Cash/Direct Debit For the payment via transfer method, kindly follow the details as shown below: Beneficiary Name : AIMST SDN. BHD. A/C 2 Account Number : 8006376336 Bank Name : CIMB Bank Berhad Bank Branch : Wisma Ria, Sungai Petani Bank Address : CIMB Bank Berhad, Wisma Ria, Taman Ria, 08000 Sungai Petani, Kedah Darul Aman, Malaysia SWIFT Code : CIBBMYKL

Please scan the inter-bank transfer receipt and email To: [email protected] and [email protected] OR Fax To: +604-429 8009 (on fax write: Attention to RC4Bs 2016 Secretariat)

All abstracts must be prepared by following the 3rdRC4Bs-2016 guidelines. Abstract and completed registration form submissions must be emailed to [email protected] and [email protected] on or before February 29, 2016 (for Early bird registration) and before March 21, 2016 for registration.  Abstract Should be written in English.  The Title should not be more than 15 words.  Abstract should not be more that 250 words, excluding title, author names and affiliations.  It is necessary to have 4-6 keywords below to the abstract.  Abstract must include 4 major sections such as Background, Methods, Results, and Conclusions.  The Organizing Committee reserves the rights to edit the Title and Abstract to maintain the quality and or consistency.  The abstract should be original and not be submitted previously to other conferences/meetings.  The presenting author’s name must be underlined. Addition of new author after the submission and acceptance of the abstract is not encouraged.  The presenting author must register to the conference. The abstract of unregistered candidates will not be processed for publication in the ‘Abstract Book’.  The presenting author is responsible for the accuracy of scientific contents of the submitted abstract. The organizing committee is not responsible for the results and conclusion provided in the abstract.

For further information OR To Become a Sponsor of This Conference

Final Announcement

3rd Regional Conference on Biosensor, Bio-diagnostics, Biochips and Biotechnology 2016 (3rdRC4Bs-2016) Achievements and Challenges in Biosensors, Bio-diagnostics, Biochips and Biotechnology

20th – 22nd April 2016 AIMST University, Semeling, Bedong Kedah, Darul Aman, Malaysia Jointly Organized by

Visit http://www.aimst.edu.my/3rdRC4Bs-2016/ OR Contact: Secretariat, 3rdRC4Bs-2016 Faculty of Applied Sciences, AIMST University, 08100 Semeling, Kedah Darul Aman, MALAYSIA. Email: [email protected] / [email protected] 1

Supported By

About (3rdRC4Bs-2016) Welcome to the 3rd Regional Conference on Biosensor, Biodiagnostics, Biochips and Biotechnology 2016 (3rdRC4Bs-2016). This conference is organized by the AIMST University (Malaysia) in collaboration with King Mongkut's University of Technology Thonburi (Thailand), Universiti Malaysia Perlis (Malaysia) and Universiti Teknologi MARA (Malaysia). Conference will be held at the AIMST University, Kedah, Malaysia from 20 – 22nd April 2016. The conference intends to provide a platform for scientists, researchers and students to share knowledge, new challenges and recent advances in the multidisciplinary areas of biosensors, biodiagnostics, biochips and biotechnology.

Objectives of the conference The conference intends to provide a platform for scientists, researchers and students to share knowledge, new challenges and recent advances in the multidisciplinary areas of biosensors, biodiagnostics, biochips and biotechnology. The 3rdRC4Bs-2016 welcomes academicians, researchers, students, entrepreneurs and industry players to present their work in a friendly and supportive environment. International and local speakers in the forefront of biosensors, biodiagnostics, biochips and biotechnology will actively engage in this 3-days event.

Scope of the Conference The scope of the conference includes but not limited to 

Biosensors

Bio-diagnostics Biochips Biotechnology Applications of Biosensors, Bio-diagnostics and Biochips in Biotech industry  Current Challenges in Biotech industry in using Biosensors, Bio-diagnostics and Biochips  Emerging fields in the Biosensors, Bio-diagnostics, Biochips and Biotechnology    

Keynote Address and Plenary / Invited Talks

Program The tentative program of the conference

Day 1: Wednesday, 20 th April 2016 7.45 pm: Registration & Welcome Reception for Participants, Orientation Hall, AIMST University 9.00 am - 5.00 pm - Preconference Workshops

Day 2: Thursday, 21 st April 2016 08.00 am – 5.30 pm: Opening Ceremony; Keynote Address: 1; Plenary Talks: 3; and paper presenter’s talk (Oral presentations): 12.

Day 3: Friday, 22 nd April 2016 08.00 am – 5.00 pm: Keynote Address: 1; Plenary Talks: 3; paper presenter’s talk (Oral presentations): 15, Young Scientist Awards (Oral and Poster category) distribution, and Concluding Remarks /Vote of thanks and Closing.

Important Dates and Deadlines Submission of the abstract

January 31, 2016

Notification of the accepted abstract

February 15, 2016

Early bird registration deadline

February 29, 2016

Submission of the full papers

March 18, 2016

Conference registration deadline

March 21, 2016

Conference date

April 20-22, 2016

Note: To download Registration form visit the conference website. 2

Pre Conference Workshops Wednesday, April 20, 2016, 9:00 am –5:00 pm. Registration and fee (RM 50/participant) is required. Limited seats (only 40) are available.

Workshop 1 Workshop 2

‘Drug-Protein Interaction and Personalized Medicine’ ‘Basic Statistics using Microsoft Excel and SPSS’ & ‘Basic Concept of Effective Scientific Writing and Communications’

‘Certificates will be given to the participants of the workshop and Conference’

You Can Become a Sponsor of This Conference!

For More Information OR To Become a Sponsor Contact Us Secretariat, 3rdRC4Bs-2016 Faculty of Applied Sciences, AIMST University, 08100 Semeling, Kedah, MALAYSIA. Email address: [email protected] and [email protected] Contact number: +604 429 8000 Extension: 8094/3012 Website: www.aimst.edu.my/3rdRC4Bs-2016

THE 3rd REGIONAL CONFERENCE ON BIOSENSOR, BIODIAGNOSTICS, BIOCHIPS AND BIOTECHNOLOGY 2016 REGISTRATION FORM Name (*Prof./Dr./Mr./Ms):_____________________________________________________________________________ Organisation:___________________________________________________________________________________________ Address: _____________________________________________________________________________________________ ____________________________________________________________________________________________ Tel:________________________________ ; Fax:__________________________________________________ ; Email:_________________________________________________________________ Please check the relevant boxes below and calculate total payable amount in RM/US$ A) Conference registration fee Oral Presentation Student / Post doc B) Accompanying Yes, person Yes, C) Pick-up from Bus/Train Station or Airport

Poster Presentation

Academics / Scientists

Company Representative

Participant

RM / US$

person/s will accompany me during conference.

RM / US$

please pick-up me from S.P. Bus Stop / Train Station

RM / US$

[3rdRC4Bs-2016 price is RM 13 (Normal rate is RM 20)]

Yes, please pick-up me from Penang Airport [3rdRC4Bs-2016 price is RM 80 (Normal rate is RM 120)]

RM / US$

I want to participate in one day post-conference Guided D) Post-conference Yes, Tour to Penang Island [RM 50 per participants] Guided Tour

RM / US$

Yes, I want to participate in one day post-conference Guided Tour to Langkawi Island [RM 100 per participants]

RM / US$

E) Pre-conference Yes, Workshop

I want to participate in workshop 1 [RM 50 per participant]

RM / US$

Yes,

I want to participate in workshop 2 [RM 50 per participant]

RM / US$

Total (A+B+C+D+ E) RM / US$

Y es

Title of Paper:-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Yes, I want to participate in the ‘Best Oral/Poster Presenter Award’ competition Enclosed is a bank draft/money order of RM*/US$*———————————— No.——————————— in favour of ‘AIMST SDN BHD A/C 2’ as a payment for my registration fee for the conference. *Note: Please refer to the conference website for other payment options. Signature:_________________________________________ Name: ___________________________________________________

Date:___________________

Conference Registration Fees Participant

Early bird registration

Standard registration

(before February 29, 2016) (after February 29, 2016) Local

International (US$)

(RM)

Local

International (US$)

(RM)

Academics & Scientists

600

200

700

250

Student*

300

100

400

150

Company Representatives

800

350

900

400

Accompanying person**

100

50

150

100

Spot Registration Local International (RM)

(US$)

1000

500

  

Registration fees is including GST. 15% discount for group registration (more than 3 participants from same laboratory) Registration fees cover conference materials, lunches, refreshments and a banquet dinner.

* Participants under the category of student registration are needed to submit an official letter of studentship from the respective university or a scanned copy of a student identity card and participation approval signature from the head of the department or main supervisor. All documents/letters must be in English. ** Accompanying person is entitled to have lunches, refreshments and a banquet dinner.

Accommodation Participants can reserve hotel rooms on their own. Participants interested to stay in the AIMST University Campus can reserve room(s)/apartment(s) in advance to avoid the disappointment. Pay separately for the accommodation. University-Campus Accommodation Hostel (Students residence): RM 40/day (for locals); US$ 25/day (for international participants) Guest House: RM 80/day (for locals); US$ 50/day (for international participants) Guest Apartment: RM 40/room/day (for locals); US$ 25/room/day(for international participants)

Contact Us Secretariat, 3rdRC4Bs-2016 Faculty of Applied Sciences, AIMST University, 08100 Semeling, Kedah, Malaysia. Email address: [email protected] and [email protected] Contact number: +604 429 8000 Extension: 8094/3012 Website: www.aimst.edu.my/3rdRC4Bs-2016