Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
Scanning Electron Microscopy for the Life Sciences Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution, and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high-resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and focused ion beam milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM, and highlights several advances in cell and molecular biology that have greatly benefited from using conventional, cryo-, immuno-, and high-resolution SEM. Heide Schatten is Professor at the University of Missouri, Columbia. Her publications include advanced imaging methods, cellular and molecular biology, cancer biology, reproductive biology, microbiology, and space biology. The latter included collaborations with NASA scientists and experiments aboard the Space Shuttle Endeavour to examine the effects of spaceflight on cytoskeletal organization during development. She has received numerous awards including grant awards from NASA, NIH, and NSF. She has published over 200 papers and seven book chapters, and edited several special topics journal issues and eight books, with several more in progress.
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
Advances in Microscopy and Microanalysis
Series Editors Patricia Calarco, University of California, San Francisco Michael Isaacson, University of California, Santa Cruz Series Advisors Bridget Carragher, The Scripps Research Institute Wah Chiu, Baylor College of Medicine Christian Colliex, Université Paris Sud Ulrich Dahmen, Lawrence Berkeley National Laboratory Mark Ellisman, University of California, San Diego Peter Ingram, Duke University Medical Center J. Richard McIntosh, University of Colorado Giulio Pozzi, University of Bologna John C. H. Spence, Arizona State University Elmar Zeitler, Fritz-Haber Institute Books in Series Published Heide Schatten, Scanning Electron Microscopy for the Life Sciences Forthcoming Nigel Browning et al., Dynamic Transmission Electron Microscopy Michael Isaacson, Microscopic Nanocharacterization of Materials Richard Leapman, Energy Filtered Electron Microscopy and Electron Spectroscopy
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
Scanning Electron Microscopy for the Life Sciences HEIDE SCHATTEN University of Missouri
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
cambridge university press Cambridge, New York, Melbourne, Madrid, Cape Town, Singapore, São Paulo, Delhi, Mexico City Cambridge University Press The Edinburgh Building, Cambridge CB2 8RU, UK Published in the United States of America by Cambridge University Press, New York www.cambridge.org Information on this title: www.cambridge.org/9780521195997 © Cambridge University Press 2013 This publication is in copyright. Subject to statutory exception and to the provisions of relevant collective licensing agreements, no reproduction of any part may take place without the written permission of Cambridge University Press. First published 2013 Printed and bound in the United Kingdom by the MPG Books Group A catalog record for this publication is available from the British Library Library of Congress Cataloging in Publication data Schatten, Heide. Scanning electron microscopy for the life sciences / Heide Schatten. p. cm. Includes index. ISBN 978-0-521-19599-7 1. Biology – Methodology. 2. Scanning electron microscopy. I. Title. QH324.9.X2S33 2013 570.280 25–dc23 2012015496 ISBN 978-0-521-19599-7 Hardback Cambridge University Press has no responsibility for the persistence or accuracy of URLs for external or third-party internet websites referred to in this publication, and does not guarantee that any content on such websites is, or will remain, accurate or appropriate.
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
Contents
Endorsements List of contributors 1
page vii ix
The role of scanning electron microscopy in cell and molecular biology: SEM basics, past accomplishments, and new frontiers
1
Heide Schatten
2
Corrosion casting technique
16
Jerzy Walocha, Jan A. Litwin, and Adam J. Miodon´ski
3
Revealing the internal structure of cells in three dimensions with scanning electron microscopy
33
Sol Sepsenwol
4
Mitochondrial continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy
50
T. Naguro, H. Nakane, and S. Inaga
5
Is the scanning mode the future of electron microscopy in cell biology?
71
Paul Walther, Christopher Schmid, Michaela Sailer, and Katharina Höhn
6
High-resolution labeling for correlative microscopy
83
Ralph M. Albrecht, Daryl A. Meyer, and O. E. Olorundare
7
The use of SEM to explore viral structure and trafficking
99
Jens M. Holl and Elizabeth R. Wright
8
High-resolution scanning electron microscopy of the nuclear surface in Herpes Simplex Virus 1 infected cells
115
Peter Wild, Andres Kaech, and Miriam S. Lucas
9
Scanning electron microscopy of chromosomes: structural and analytical investigations
137
Elizabeth Schroeder-Reiter and Gerhard Wanner
10
A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy
165
Giuseppe Familiari, Rosemarie Heyn, Luciano Petruzziello, and Michela Relucenti
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
vi
Contents
11
Scanning electron microscopy of cerebellar intrinsic circuits
179
Orlando J. Castejón
12
Application of in vivo cryotechnique to living animal organs examined by scanning electron microscopy
196
Shinichi Ohno, Nobuo Terada, Nobuhiko Ohno, and Yasuhisa Fujii
13
SEM in dental research
211
Vladimir Dusevich, Jennifer R. Melander, and J. David Eick
14
SEM, teeth, and palaeoanthropology: the secret of ancient human diets
236
Alejandro Romero and Joaquín De Juan
Index
257
The color plates are to be found between pages 118 and 119.
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
Endorsements
“This book is an excellent exposition of the many-faceted field of biological scanning electron microscopy. A brief introduction to the physics of SEM imaging is followed by an outstanding selection of recent applications, which are written by leaders in their respective fields and which include complete methodological details.” Michael Marko, Wadsworth Center, New York State Department of Health “This book, Scanning Electron Microscopy for the Life Sciences, edited and compiled by Dr. Heide Schatten, comprises an extensive collection of articles demonstrating that the relevance of SEM to biological research is of increasing importance. The book is a very valuable compendium for any researcher interested in the fine structural morphology and chemistry of the cell and its compartments, such as mitochondria, and the nucleus and its contents. This, combined with the wide array of approaches, including recent ones, such as helium ion microscopy and block-face imaging combined with serial sectioning inside the SEM chamber, are all covered in this well-illustrated and also otherwise beautiful produced volume.” Bert Menco, Northwestern University “Scanning Electron Microscopy for the Life Sciences includes an outstanding array of chapters on techniques for sample preparation and SEM imaging for a number of specimen types ranging from entire organs to molecules. Chapters include detailed information on protocols for specimen preparation and data collection, analysis, and presentation that have been applied to specific biological systems. Even though the information presented is specific to the experimental systems used in the laboratories of the authors, the methods and information provided will benefit all who use SEM in their research.” Robert Price, University of South Carolina School of Medicine “The use of scanning electron microscopy in the life sciences has increased dramatically in the last decade. This has given rise to advances in equipment and development of new techniques. This text provides a needed survey of these advances; displaying the many ways that the beautiful threedimensional structural detail available with scanning electron microscopy can be exploited. Scanning electron microscopy has long been prized for its ability to visualize high resolution surface detail, but many of the chapters in this book also show how internal detail of cells and tissue can be analyzed by scanning electron microscopy. This is an excellent introductory text for those who want to incorporate scanning electron microscopy in their repertoire. However, the wellwritten descriptions of cutting-edge techniques and the many ‘tips and tricks’ provided by expert authors insure that even experienced scanning electron microscopists will find the book valuable.” W. Gray Jerome, Vanderbilt University Medical Center
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viii
Endorsements
“This book is an excellent source of information about recent advances in the field of SEM for the life sciences and will assist microscopists in gaining a greater depth of understanding.” Cynthia S. Goldsmith, Centers for Disease Control and Prevention (CDC) “Authoritative review of modern biological SEM methods. Advanced specimen preparation and imaging methods reveal fine details not observable by other means.” Charles Lyman, Lehigh University
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Cambridge University Press 978-0-521-19599-7 - Scanning Electron Microscopy for the Life Sciences Heide Schatten Frontmatter More information
Contributors
Ralph M. Albrecht University of Wisconsin-Madison Orlando J. Castejón Universidad del Zulia, Venezuela Joaquin De Juan Universidad de Alicante, Spain Vladimir Dusevich University of Missouri – Kansas City J. David Eick University of Missouri – Kansas City Giuseppe Familiari Sapienza University of Rome, Italy Yasuhisa Fujii University of Yamanashi, Japan Rosemarie Heyn Sapienza University of Rome, Italy Katharina Höhn University of Heidelberg, Germany Jens M. Holl Emory University School of Medicine, Atlanta S. Inaga Tottori University, Japan
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x
List of contributors
Andres Kaech University of Zürich Jan A. Litwin Jagiellonian University Medical College, Krakow, Poland Miriam S. Lucas Swiss Federal Institute of Technology, Zürich Jennifer R. Melander University of Missouri – Kansas City Daryl A. Meyer University of Wisconsin-Madison Adam J. Miodonski Jagiellonian University Medical College, Krakow, Poland T. Naguro Tottori University, Japan H. Nakane Tottori University, Japan Shinichi Ohno University of Yamanashi, Japan Nobuhiko Ohno University of Yamanashi, Japan O. E. Olorundare University of Wisconsin-Madison and University of Ilorin, Nigeria Luciano Petruzziello Sapienza University of Rome, Italy Michela Relucenti Sapienza University of Rome, Italy Alejandro Romero Universidad de Alicante, Spain Michaela Sailer Universität Ulm, Germany
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List of contributors
xi
Heide Schatten University of Missouri-Columbia Christopher Schmid Max-Planck-Institute for Molecular Physiology, Germany Elizabeth Schroeder-Reiter Ludwig-Maximilians-Universität München, Germany Sol Sepsenwol University of Wisconsin, Stevens Point Nobuo Terada University of Yamanashi, Japan Jerzy Walocha Jagiellonian University Medical College, Krakow Paul Walther Universität Ulm, Germany Gerhard Wanner Ludwig-Maximilians-Universität München, Germany Peter Wild Institute of Veterinary Anatomy and Virology, University of Zürich Elizabeth R. Wright Emory University School of Medicine, Atlanta
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