Digital Imaging Solution

INTRODUCTION

TEM Camera Overview Side- and Bottom-mounted TEM Cameras

Overview Application

MegaViewG2

Digital Documentation

Morada

KeenViewG2

CantegaG2

++

++

++

++

Pathology

+

+

++

+

(+)

Virology

(+)

+

+

++

++

Immunogold Analysis

(+)

+

++

+

++

Tomography

+

++

(+)

+

++

Cryo microscopy

(+)

(+)

-

+

++

Low Dose Imaging

(+)

(+)

-

+

++

-

(+)

-

+

++

(+)

+

(+)

+

++

-

-

-

+

++

HRTEM

(+)

(+)

(+)

++

++

Diffraction

+

+

+

+

++

Convergent Beam Electron Diffraction

(+)

(+)

(+)

+

++

EFTEM Materials Science Holography

1) 35 mm port

Veleta

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Digital Cameras for Electron Microscopy

2) viewing screen

System Diagram

3) on-axis 1) 35 mm port

2) viewing screen

magnification ratio (onscreen vs. viewing screen) 35 mm port / ~ 0.25 on axis / ~ 1.3

Specifications are subject to change without any obligation on the part of the manufacturer. Soft Imaging System is a trademark of Olympus Soft Imaging Solutions GmbH.

www.olympus-sis.com www.soft-imaging.net

Johann-Krane-Weg 39 48149 Münster Germany Phone: +49 (251) 7 98 00-0 Fax: +49 (251) 7 98 00-6060 [email protected]

No. 2 Jurong East Street 21 #05-30, IMM Building, Singapore 609601 Singapore Phone: +65 (6777) 7898 Fax: +65 (6777) 7218 [email protected]

[email protected]

Art. code: 1-061-201-08-1 • 05/2008

3) on-axis

INTRODUCTION

DIGITAL CAMERAS FOR ELECTRON MICROSCOPY

BOTTOM-MOUNTED TEM CCD CAMERAS

SOLUTIONS FOR AN EFFICIENT TEM WORKDAY Reliable, reproducible and objective

A

Specifications

CantegaG2 Fiber-optically coupled 2k x 2k bottom-mounted TEM Camera

Using digital technology in the field of transmission electron microscopy (TEM) offers unique and attractive advantages. With the right digital camera and software, image acquisition, analysis, storage and documentation become much easier compared to conventional analog approaches. The latest developments guarantee the same quality as was available with the familiar photo plate. Steadily rising pressures to cut costs along with increasing quality expectations can be dealt with effectively. Digital solutions save time and money and reduce errors. And working digitally is a lot more user friendly. Each of the cameras we have developed reflects the current requirements of industry, biomedicine, life sciences and the materials-sciences. This is true for all of our TEM cameras – both the 35 mm port side-entry cameras and bottom-mounted cameras. Properties such as high resolution, high frame rates, excellent signal-to-noise ratio, wide dynamic range, easy installation, interactive and automatic on-screen focusing and automatic image enhancement all guarantee that your TEM workday is efficient. Practice-oriented – iTEM, the TEM imaging platform In addition to their quality and affordability, all cameras are fully integrated with our user-friendly TEM software environment iTEM, and fully operable via the software. In most cases, remote-controlled TEMs and motorized stages can be operated via iTEM. Integrated real-time functions such as automatic contrast enhancement ensure the best possible image quality during acquisition. Software extensions tailored toward your applications make it easy to increase the range of functions offered by iTEM. These include acquisition, evaluation and visualization of energy-loss images and spectra, evaluation of diffraction images, determination of mechanical using CBED image series, acquisition, reconstruction and visualization of tomography series and automated particle analysis.

Veleta

10.2 mm x 8.3 mm (2/3“),

16.67 x 16.67 mm

36 mm x 24 mm

10.2 mm x 8.3 mm (2/3“),

28.67 mm x 28.67 mm

interline CCD image sensor

interline CCD image sensor

interline CCD image sensor

interline CCD image sensor

full frame CCD image sensor

Resolution (pixel)

1376 x 1032

2048 x 2048

4008 x 2672

1376 x 1032

2048 x 2048

Binning 2x (pixel)

688 x 516

1024 x 1024

2004 x 1336

688 x 516

1024 x 1024

Pixel size (µm2)

6.45 x 6.45

7.4 x 7.4

9.0 x 9.0

6.45 x 6.45

14.0 x 14.0

Effective pixel size (µm2)

~ 20.0 x 20.0

13.1 x 13.1

9.0 x 9.0

23.5 x 23.5

14.0 x 14.0

Binning

2x, 4x and 8x

2x, 4x and 8x

2x, 4x and 8x

2x, 4x and 8x

2x, 4x and 8x

Pixel clock rate

24.6

24.6

24.6 (live)

24.6

2.0 (high quality mode)

Chip type

(MHz) Frame rate (fps) Frame rate (fps)

Morada

KeenViewG2

12.3 (snapshot) > 15

@ full resolution

> 5 (12 bit)

CANTEGAG2 - 2K X 2K BOTTOMMOUNTED TEM CCD CAMERA

5.0 (viewing / high speed mode) > 15

~ 0.4 (high quality mode) ~ 1.5 (high quality mode) (4x)

~ 5 (14 bit)

~ 1 (high quality mode)

> 25 (2x)

> 20 (4x)

> 3 (2x)

> 25 (2x)

High quantum efficiency The CCD sensor provides 2048 x 2048 pixel resolution with a 14-bit dynamic range. The full-frame CCD chip offers a pixel size of 14 µm x 14 µm with an active area of 28.7 mm x 28.7 mm. With a dark current of < 0.6 e-/p/s, a full well capacity of about 250,000 electrons, a read-out noise of approximately 8 electrons and its water-cooled system, the high sensitivity of the CantegaG2 allows observation of samples on the monitor at beam intensities so low, they would normally prohibit a user seeing anything at all on the TEM viewing screen. Convenient frame rates The CantegaG2 supports two readout rates at 14 bits. The 2 MHz mode produces images of the highest quality at convenient frame rates. In the high speed 5 MHz mode the camera offers about 6 frames per second at binning 4 and 1 frame per second at full resolution. At any resolution, this camera provides a superb dynamic range and sensitivity. Binning modes of 2x, 4x and 8x are supported by the CantegaG2. This means that sensitivity can be increased even more.

~ 6 (high quality mode) (4x)

Features

Digitization (bit)

14

14

14

14

14

Up to 2048 x 2048 pixels

Exposure time

100 µs - 160 s

100 µs - 160 s

1 ms - 60 s

100 µs - 160 s

1 ms - 100 s

Up to 6 fps @ binning 4

Display

Full image in real time

Full image in real time

Full image in real time

Full image in real time

Full image in real time

USB 2.0 interface

Chip temperature

10° C @ 25° C ambient

10° C @ 25° C ambient

15° C @ 25° C ambient

10° C @ 25° C ambient

minus 10°C @ 20° C

temperature

temperature

temperature

temperature

temperature

14 bit

Noise reduction

Correlated double sampling

Correlated double sampling

YAG Scintillator

Camera mount

at wide angle port

at wide angle port

at wide angle port

Camera positioning

PC controlled pneumatic

PC controlled pneumatic

PC controlled pneumatic

Fiber-optically coupled

The CantegaG2 is the 2nd generation in Olympus Soft Imaging Solutions 2k x 2k bottom-mounted TEM CCD camera family. This fiber-optically coupled 14-bit camera raises the bar with razor sharp images, increased uniformity and high sensitivity. The CantegaG2 offers an optimized high-quality fiber-optically coupled 2k x 2k bottom-mounted TEM Camera.

CantegaG2

up to 2

@ binning

Applications

Innovative system approach The CantegaG2 camera system couples the electron-sensitive layer (scintillator) with the CCD chip using unique fiber optics. The CantegaG2 uses an improved YAG scintillator which provides superior uniformity and homogeneity. The camera can be freely rotated under vacuum conditions in order to align the CCD chip to the specimen feature or the tilt axis of the goniometer. The CantegaG2 offers extremely low exposure times (as little as 1 ms and up to 100 s), making acquisition viable with intense illumination and with images of very low intensity.

Adenoviruses, acquired with the Morada side-mounted TEM camera at 120,000x

MegaViewG2

Correlated double sampling on axis

on axis, rotatable

movement / control box

movement / control box

movement / control box

CBED Analysis

Anti blooming

> 300x

> 300x

> 100x

> 300x

yes

Immunogold Labelling

PC interface

FireWireTM (IEEE 1394)

FireWireTM (IEEE 1394)

FireWireTM (IEEE 1394)

FireWireTM (IEEE 1394)

USB 2.0

Tomography

Camera coupling

Lens-optically coupled

Lens-optically coupled

Lens-optically coupled

Fiber optics (tapered)

Fiber optics (1:1)

Diffraction

Trigger Option

no

yes

In & Out

no

yes

Cryo Microscopy

Partial Readout

no

yes

yes

no

yes

EFTEM

Full Well Capacity (e-)

24000

40000

~ 60000

24000

250000

Virology

Dark Current (e-/s)

n/a

~ 40

n/a

n/a

< 0.6

Materials Science

Readout Noise (e-)

< 10

< 20

< 30

< 10

~8

Digital Documentation

Scintillator

High sensitivity phosphor

High sensitivity phosphor

High sensitivity phosphor

High sensitivity phosphor

YAG

optimized for accelerating

optimized for accelerating

optimized for accelerating

optimized for accelerating

Low Dose Imaging Holography

voltage for 100 keV / 200 keV

voltage for 100 keV / 200 keV

voltage for 100 keV / 200 keV

voltage for 100 keV / 200 keV

CE certified

yes

yes

yes

yes

yes

RoHS compliant

yes

yes

yes

yes

yes

Field of view

B

Iron mapping (ESI) - Liver Cell

C

Chilled ”Dark current“ (thermal) noise is not a problem with the CantegaG2 due to a sophisticated Peltier-cooling system which regulates the temperature of the CCD chip down to -10° C. Germanium (Ge)

SIDE-MOUNTED TEM CCD CAMERAS

A

MegaView G2 Fast side-mounted TEM Camera

Features

MEGAVIEW - FAST SIDE-MOUNTED TEM CCD CAMERA G2

Up to 1376 x 1032 pixels More than 25 fps @ binning 2 Peltier cooled Lens coupled 14-bit

A The MegaView is the 2nd generation of the Olympus Soft Imaging Solutions high resolution and high speed read-out side-mounted TEM CCD camera system. This lens-optically coupled 14-bit camera offers a comprehensive concept composed of the latest in electronics, optics, design and functionality. The MegaViewG2 is equally attractive for users in the bio-medical, life sciences, and materials science fields. G2

Well-balanced design The MegaViewG2 consists of a high yield phosphor screen, a prism to reflect the image onto the CCD sensor, as well as housing and flange specific to fit almost every TEM. The rigid coupled phosphor screen-lens-scintillator-CCD combination provides optimum conditions for artifact-free shading correction. This single port design is important for obtaining reproducible images. The positioning of the phosphor screen inside the TEM is pneumatically driven. X-Ray safety is guaranteed up to 200 KeV.

Applications Life Sciences Pathology Diffraction Materials Science Digital Documentation

B

Optimized optics The MegaViewG2 camera system is equipped with a specially designed lens. In conjunction with the CCD chip and the increased efficiency scintillator, the optimized optics of the MegaViewG2 system ensure great sensitivity and light efficiency, as well as a high signal-to-noise ratio. Superlative CCD sensor The highly sensitive 2/3” interline CCD image sensor used in the MegaViewG2 provides resolution of 1376 x 1032 pixels with 14-bit dynamic range (grey values). The pixel size of the CCD chip is 6.45 x 6.45 µm2 (effective size). The CCD sensor possesses high quantum efficiency, which in combination with reduced dark current and 10° C cooling, results in an increase of the detection quantum efficiency (DQE) of the camera system. Various imaging modes Several binning modes are supported by the MegaViewG2. The users can select between modes of 2x, 4x and 8x binning. This offers sensitivity that can be increased even more. The interline chip uses an electronic shutter which prevents any possible mechanical problems. The MegaViewG2 offers exposure times ranging from 100 µs to 160 s. This is a fundamental prerequisite for being able to acquire images of either intense illumination or of very low intensity. The MegaViewG2 supports frame rates of more than 25 images per second at binning 2 and of more than 15 images per second at full resolution. This high frame rate is ideal for locating suitable sample segments directly onscreen or adjusting the microscope parameters. At any resolution the camera provides excellent dynamic range and sensitivity.

Adenovirus

C

SAD image of small Al crystalllite, indexing by use of iTEM Solution Diffraction

SIDE-MOUNTED TEM CCD CAMERAS

A

Veleta Lens-optically coupled 2k x 2k side-mounted TEM Camera

Features Up to 2048 x 2048 pixels Peltier cooled Lens coupled 14-bit Applications Tomography Diffraction Materials Science Virology Pathology Digital Documentation

VELETA - 2K X 2K SIDE-MOUNTED TEM CCD CAMERA A The Veleta is a side-mounted TEM camera offering a peltier-cooled CCD chip with 2k x 2k pixels and a 14-bit dynamic range. The camera fits perfectly into our product line of sophisticated, side-mounted TEM cameras – the successful and highly recommended MegaView series and the 11 MegaPixel Morada. The Veleta reflects current life science, bio-medicine, materials science and industrial requirements.

Highly sensitive CCD sensor The new, highly sensitive CCD chip used in the Veleta provides maximum resolution of 2048 x 2048 pixels with a 14-bit dynamic range. In conjunction with further dark current reduction measures, this increases the detection efficiency of the entire camera system tremendously. The CCD sensor possesses uniquely high quantum efficiency, which in combination with reduced dark current results in a tremendous increase of total detection quantum efficiency (DQE) of the camera system.

B

Liver cell - nucleus

C

Wide range of exposure times The interline transfer chip uses an electronic shutter. This removes any possibility for mechanical problems. The Veleta offers exposure times ranging from 100 µs to 160 s. This is a fundamental prerequisite for being able to acquire images of either intense illumination or of very low intensity. The CCD chip’s interline architecture guarantees extremely high anti-blooming. Individual pixels are clearly distinguished. This, in conjunction with extremely short exposure times, optimizes the acquisition of diffraction images. Sensitivity based on high light efficiency Due to a partnership with the best lens makers, the Veleta camera system has been equipped with a new, specially designed optical lens. The custom optics of the Veleta and more efficient scintillators ensure great sensitivity and light efficiency, as well as a high signal-to-noise ratio.

Diffraction image of aluminium

Excellent dynamic and high frame rates The Veleta supports frame rates of more than 20 images per second at binning 4 and of 5 images per second at full resolution. This high frame rate is ideal for locating suitable sample segments directly onscreen. In conjunction with the RT-FFT (Real Time Fast Fourier Transformation), the focusing mode offers ideal assistance for setting various microscope parameters. This enables you to conveniently focus your sample on your PC screen as opposed to having to use the viewing screen of the microscope. No matter what mode you select, the Veleta offers excellent dynamic range and high sensitivity.

SIDE-MOUNTED TEM CCD CAMERAS

A

Morada The camera offers a field of view comparable to the size of a photo plate

Features Up to 4008 x 2672 pixels

MORADA - 11 MEGAPIXEL SIDE-MOUNTED TEM CCD CAMERA

Up to 10.3 fps Water cooled Lens-optically coupled 14 bit Applications

A The Morada is a side-mounted TEM camera offering a water-cooled CCD chip

Immunogold Labelling

with up to 11 MegaPixels and a 14-bit dynamic range. This is a TEM camera for the wide-angle port of all commonly used transmission electron microscopes. It offers superior properties both for biomedical and materials science applications. Highly precise mechanics The camera system is comprised of a highly efficient lens coupled phosphorous screen, a specially developed lens for projecting the image onto the CCD sensor, and precision mechanics. The camera offers a level of detail comparable to a photo plate as well as twice the field of view.

Diffraction Virology Pathology Digital Documentation

B

Broad range of application fields The CCD chip provides a maximum resolution of 4008 x 2672 pixels (depending on TEM). The CCD chip is stabilized at a temperature of 15° C. Noise is further reduced via innovative digital read-out technology. The result is high sensitivity and efficiency as well as an extraordinary signal-to-noise ratio. The CCD chip of the Morada has an electronic shutter. Extremely fast exposure times - less than 1 ms - are viable, along with extremely lengthy ones - up to 60 s. This ensures perfect acquisition of your specimen no matter whether current conditions require a long or very brief exposure time. Various imaging options The camera also supports various binning modes, thus enhancing sensitivity even more. The Morada uses a maximum pixel clock rate of 24 MHz. This makes readout rates of up to 10 frames per second in the partial read-out mode possible. This mode enables users to search specimens conveniently at their PCs for relevant detail as well as being able to set various microscope parameters. For the Snapshot, the Morada automatically switches to the 12-MHz mode. This ensures further noise reduction and perfect image acquisition. The Morada camera also offers trigger functionality. Flexible capabilities The Morada uses new, specially designed scintillators, thereby fulfilling customers‘ requirements. These scintillators are optimized for 100 keV and 200 keV, in accordance with customer specifications. Acquired images are transferred to the PC via FireWire™ (IEEE 1394a) technology.

Human Brain

C

CBED

BOTTOM-MOUNTED TEM CCD CAMERAS

A

KeenViewG2 Finely balanced concept of electronics, optics, design and functionality

Features Up to 1376 x 1032 pixels More than 25 fps @ binning 2 Peltier cooled Fiber-optics (tapered) 14 bit No water cooling or external shutters necessary Applications Diffraction Tomography EFTEM Virology Materials Science

KEENVIEWG2 - FAST BOTTOMMOUNTED TEM CCD CAMERA A The KeenViewG2 is the 2nd generation of Olympus Soft Imaging Solutions high resolution and high read-out speed bottom-mounted TEM CCD camera system. This fiber-optically coupled 14-bit camera sets benchmarks with its finely balanced concept of electronics, optics, design and functionality. The KeenViewG2 is equally attractive for users in the bio-medical, life sciences, materials science and semiconductor fields.

Digital Documentation

Powerful sensor The highly sensitive 2/3” interline CCD image sensor used in the KeenViewG2 provides a resolution of 1376 x 1032 pixels with 14-bit dynamic range (16384 grey values). The pixel size of the CCD chip is 6.45 x 6.45 µm2 (effectively 23.5 x 23.5 µm2). Sophisticated design The KeenViewG2 camera system couples a high-sensitivity phosphor scintillator with the CCD chip using unique tapered fiber optics. This innovative approach provides the user with an enviable price/performance advantage. In order to match customers’ individual demands various scintillators can be customized for several accelerating voltages. The CCD chip architecture guarantees extremely high anti-blooming. Individual pixels are clearly distinguished. In conjunction with the extremely short exposure times, this optimizes acquisition of diffraction images. In conjunction with the highly sensitive CCD chip and the optimized scintillator, this ensures high sensitivity and efficiency.

B

Adenovirus

C

HRTEM-Carbon Black

Flexible frame rates The KeenViewG2 supports frame rates of more than 25 images per seconds at binning 2 and of 15 images per seconds at full resolution. This high frame rate is ideal for locating suitable sample segments directly onscreen. Several binning modes are supported by KeenViewG2. The users can select between modes of 2x, 4x and 8x binning. This means sensitivity can be increased even more. The interline chip uses an electronic shutter which avoids any possible mechanical problems. The KeenViewG2 offers exposure times ranging from 100 µs to 160 s. This is a fundamental prerequisite for being able to acquire images of intense illumination or of very low intensity. Chilled Thermal noise (“dark current”) is not a problem with the KeenViewG2 due to a sophisticated Peltier-cooling system which regulates the temperature of the CCD chip at 10° C (@ 25° C ambient temperature).