J. AMER. SOC. HORT. SCI. 128(4):584–590. 2003.

Development and Characterization of Microsatellite Markers in Citrus Riaz Ahmad, Darush Struss, and Stephen M. Southwick1 Pomology Department, University of California, Davis, CA 95616 ADDITIONAL INDEX WORDS. molecular markers, SSR, DNA finger printing, cultivar identification ABSTRACT. We evaluated the potential of microsatellite markers for use in Citrus genome analysis. Microsatellite loci were identified by screening enriched and nonenriched libraries developed from ‘Washington Navelʼ Citrus. Microsatellite-containing clones were sequenced and 26 specific PCR primers were selected for cross-species amplification and identification of cultivars/clones in Citrus. After an enrichment procedure, on average 69.9% of clones contained dinucleotide repeats (CA)n and (CT)n, in contrast to