782014

Demir }Oklemesinin plazrna bakJ r w yink> dOZe}'lerine etkisi; Se}men w ark

DEMiR YUKLEMESiNiN PLAZMA BAKIR VE CiNKO DUZEYLERiNE ETKiSi*

Ce·rrahpa§a Tip Dergisi

Hakk1 Oktay SEYMEN, Murat MENGi, Dervif OZCELiK, Tevfik GULYA~AR, Pmar SEYMEN, GOnnur YiGiT Background and design.- The harmful effects of acute and chronic iron loading are well documented. Increased lipid peroxidation is an important expression of both chronic and acute iron toxicity. In the structure of some antioxidant enzymes such as SOD, the copper and the zinc are im.ulved. Our aim was to study the effect of iron overloading on the plasma copper and zinc levels. Results.- Adult male Wistar albino rats weighing 200-205g were used. The animals were divided into 2 weight-matched groups. Group 1: Control group (n=6) and Group 2: Iron overloading group (n=7). The rats of group 2 received intraperitoneal iron (Ferro Ill hydroxide polymaltose) at a dose of 250 mg/kg/day for 10 days. At the end of the administration period, the rats were killed under ketamine ariesthesia (50 mg/kg). Heparinized blood samples were obtained from aorta abdominalis. The plasma iron, copper and ziric levels were mesaured by atomic absorbtion spectrophotometer. The plasma free iron values for rats receiving iron were significantly (p d0ze}4erine etkisi; Seyren w erk.

7 82014

reaksiyonlannda da g0riilrrektedir. Cu++ 02 -->Cu+++ 02 -. Membran lipidlerinde peroksidasyon ~levi hidrosil, alkoksi ve peroksi radikalleri gibi reaktif maddelerin bir !llltilen grubundan hidrojen atollllllU 91karmas1 ile geiyekle~ir. 4 ) hpDelen ilo ymo her olmto DYiUJl ha-cllllm Pl lmnfm, ailil mlllia HCL vo BOO (Baok ted damage to deoxynbose and benmate. BiochemJ 1987; 243: 709-714. 3. Hershko C. Mechanism of iron toxicity and its possible role in red cell ~mbrane damage. Seminars in Heimtology,1989; 26: 277-285. 4. Halliwell B. Free radicals, antioxidants and human disease: curiosity, cause, or consequance. Lancet 1994; 334: 721-724. 5. Tanakol R Antioksidan vitaminler: Hastahkta ve saghkta C>nemleri. Klinik Ge~iin.1998; 11: 347-357. 6. Seymm 0, Seven A, Candan G, Yigit G, Hatemi S, Hrumti H. The effect of iron supple~htation on rnH levels, GSH-Px, and SOD activities of erythrocytes in L-Thyroxine administration. Acta Med Okayama 1997; 51: 129-133. 7. Agil A, Fuller CJ, Jialal I. Susceptibility of plasma to ferrous iron/hydrojen peroxide-Dl.'diated oxidation: demmstration of a possible Fenton Reaction. Clin Chem 1995; 41: 220-225. 8. Sempos CT, Looker AC, Gillum RF, Mak.uc DM. Body iron stores and the risk of coronary heart disease. N Fng J Med 1994; 30: 1119-1124. 9. Barut~u UB, Ak.yolcu MC, Kiziler AR, Oke N, Toplan S, .Karakoc Y, Onen S. Effects of coronary artery diseases on haem>rheological p~ters and trace elemmts . J Basic Clin Physiol Phannacol 1995; 6: 289-294. 10. Rosario Z, Hidalgo FJ, Tappel AL Comparative antioxidant effectiveness of dietary ~-carotene, vitamin E, Selenium and Coenzyme Q 10 in rat erythrocytes and plasma. JNutr 1991; 121: 50-56.

• Anahtar Kelimeler: Oksidan stres, demir, baktr, ~inko; Key Words: Oxidant stress, iron, copper, zinc; Almdzgz Tarih: 28 Eyliil 1998; D~. Dr. Hakla Oktay Seymen, Bio. Murat Mengi, Prof. Dr. Giinnur Yigit: tO. Cerrahpa~a T:tp Fakiiltesi Fizyoloji Anabilim Dab; Dr. De~ Oz.yelik, Di-. Tevfik Giilya~ar: i:O. Cerrahpa~a T:tp Fakiiltesi Biyofizilc Anabilim Dab; Dr. Pmar Seymen: T.C. Haydarpa~a Nwmme Hastanesi 2. Dahiliye K1inigi Yazl§ma Adresi (Address): Dr. H.O. Seymen, tO. Cerrahpa~a T:tp FakOltes~ FizyoJoji Anabilim Dab, 34303, Cerrahpa~a, istanbul. http://www.clf.edu.tr/dergi/onlinel1999IOO's2/99s2a1ft.htm

617

782114

GD

Cerrahpa!?a Journal of Medicine

~

1n