Vito Quaranta, MD

VITO QUARANTA, M.D. Professor of Cancer Biology Director, Center for Quantitative Systems Biology PI, Center for Cancer Systems Biology @ Vanderbilt Vanderbilt University School of Medicine 446B Preston Research Building Nashville, TN 37232-6840 Telephone: 615-936-2868 Facsimile: 615-936-2911 [email protected] https://labnodes.vanderbilt.edu/community/profile/id/334 http://ccsb.vanderbilt.edu/QSBCenter/ CURRICULUM VITAE (updated February 2016) PERSONAL: Date of Birth: November 6, 1950 Place of Birth: Bari, Italy Nationality: U.S.A. EDUCATION: 1977-1981 1977 1976 1975 1974

Postdoctoral Fellow, Molecular Immunology, The Scripps Research Institute, La Jolla, CA Board of Hematology, University of Bari Medical School, Bari, Italy Board of Educational Commission for Foreign Medical Graduates, U.S.A. Medical License, Italy M.D. Degree, University of Bari Medical School, Bari, Italy

GRADUATE AND POSTDOCTORAL ADVISORS (current positions): Professor Lorenzo Bonomo, University of Rome Medical School, “La Sapienza” (retired) Ralph Reisfeld, Ph.D., The Scripps Research Institute, La Jolla, CA Soldano Ferrone, MD, Ph.D., Massachusetts General H., Harvard U., Cambridge, MA Per A. Peterson, Senior V. President, Johnson&Johnson, New Brunswick, NJ (retired) POSITIONS: 2003-present Professor, Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 1992-2003 Tenured Associate Professor, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 1987-1992 Associate Member, Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 1981-1987 Assistant Member, Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 1977-1981 Research Associate, Department of Molecular Immunology, Research Institute of Scripps Clinic, La Jolla, CA 1974-1977 Research Fellow (Clinical & Research) Department of Internal Medicine, University of Bari Medical School, Bari, Italy LEADERSHIP: 2015-present

Director, Center for Quantitative Systems Biology, Vanderbilt U

Vito Quaranta, MD

2004-present 2012-present 2005-2011 2003-2009

PI, Center for Cancer Systems Biology @ Vanderbilt (Center grant from the NCI Integrative Cancer Biology Program) Executive Committee Member, Vanderbilt Center for Quantitative Sciences Co-Chair, Integrative Cancer Biology Program, NCI Co-Director, Center for Matrix Biology, Vanderbilt University

ADVISORY: 2010-present - University of Minnesota Masonic NCI Comprehensive Cancer Center, Member, Scientific Advisory Board 2015-present - Center for Mathematical Modeling and Data Science, U Osaka, Japan, External Advisory Board 2016 - Co-Organizer, q-bio Conference, Nashville, TN 2013-2017

- Conference on Computational Cell Biology, Cold Spring Harbor, NY, Co-Organizer 2014 - Organizing Committee Member, Cancer Modeling Emphasis Year, Mathematical Biosciences Institute, Ohio State University, Columbus, OH - NCI SPORE Program, Reviewer 2013 - NCI-ICBP Workshop on Tumor Cell Heterogeneity, Nashville, TN, Co-Organizer - Internal Advisory Board, Lung SPORE, Vanderbilt University Medical Center - 29th Annual Forbeck Forum on Resistance Mechanisms, Think Tank - 9th Conference on Computational Cell Biology, Cold Spring Harbor, NY, Co-Organizer - AACR-National Brain Tumor Society Cancer Systems Biology Think Tank, Philadelphia - NCI SPORE Program, Reviewer - NCI, Site Visitor, Robert H. Lurie Cancer Center Support Grant Review, Chicago, IL - NCI, Site Visitor, Wistar Cancer Center Support Grant Review, Philadelphia, PA 2012 - Organizing Committee Member, Workshop on Mathematical Oncology IV: Integrative Cancer Biology, The Fields Institute, Toronto, Canada - NCI, Molecular Oncology P01 Special Emphasis Panel, Reviewer - Integrative Cancer Biology Program, NCI, Education Outreach Speaker, Houston, TX - NCI, Site Visitor, Helen Diller Cancer Center Support Grant Review, San Francisco, CA 2011 - NCI, Site Visitor, Emory Winship Cancer Center Support Grant Review, Atlanta, GA - National Brain Tumor Society, National Meeting, Systems Biology Discussion Panelist, Philadelphia, PA - Ontario Research Fund, Research Excellence Program, Reviewer - NCI SPORE Program, Reviewer - 8th Conference on Computational Cell Biology, Cold Spring Harbor, NY, Session Chair

Vito Quaranta, MD

2010 - University of Sharjah, United Arab Emirate, External Promotion Reviewer - USC Norris Comprehensive Cancer Center, Site Visitor - NCI, Cellular & Tissue Oncology P01 Special Emphasis Panel Reviewer - NCI, Molecular Oncology P01 Special Emphasis Panel, Reviewer - NCI EUREKA Program Review Panel, Reviewer - European Commission Directorate for Research, Systems Biology Large-Scale Projects, Key Remote Evaluator - NCI Centers of Cancer Nanotechnology Excellence Review Panel, Reviewer - College of CSR Reviewers, Editorial Board Member - NCI, Site Visitor, MIT Cancer Center Support Grant Review 2009-present - Breast Cancer Targets and Therapy, Honorary Editorial Board Member - American Journal of Translational Research, Editorial Board Member 2009 - National Cancer Institute, Molecular Oncology P01 Panel Member 2007 - Tumor Progression and Metastasis Study Section, NIH CSR, ad hoc - Pathology B Study Section, NIH, ad hoc Member - NCI, Program Projects, Cellular and Tissue Biology Cluster Reviewer - American Society of Biochemistry and Molecular Biology, Annual Meeting, Extracellular Matrix Theme Chair 2006 - Modeling and Analysis of Biological Systems Study Section, NIH CSR, ad hoc Member - American Association for Cancer Research, Annual Meeting, Symposium Co-chair 2004 - MIT Cancer Center, Site Visitor - American Society for Matrix Biology, Annual Meeting, Co-organizer 2003 - Pathology B Study Section, NIH CSR, ad hoc Member 2002-present - J. of Cellular Physiology, Associate Editor 2001-2005 - National Cancer Institute Initial Review Group, Subcommittee C – Basic and Preclinical, Member 2001 - National Cancer Institute, Site Visit Committee Member 2000 - National Cancer Institute, Special Review Panel Member - National Cancer Institute, Site Visit Committee Member

Vito Quaranta, MD

1999 - National Institute of Dental and Craniofacial Development, RFA Study Section Member 1998 - Cell Biology I Study Section, NIH, ad hoc Member 1997 - Cell Biology I Study Section, NIH, ad hoc Member 1995-1996 - Cell Biology Panel, Division of Molecular and Cellular Biosciences, National Science Foundation 1994 - Experimental Immunology Study Section, NIH, ad hoc Member - National Cancer Institute, Site Visit Committee Member 1992-2012 - Cancer Research, Associate Editor 1992 - Experimental Immunology Study Section, NIH, ad hoc Member - “Desmos, Inc.” (currently “Novocell, Inc.”), co-Founder 1991 - Keystone Symposia on Molecular and Cellular Biology “Integrins: Cell Adhesion and Transmembrane Communication in Development And Disease”, Co-organizer. HONORS and PRIZES: 2015 Fellow, American Association for the Advancement of Science 2012 Stanley Cohen Award, Outstanding Contribution for Research Bridging Diverse Disciplines 2011 Apulians in the World Prize, Outstanding Achievements Natives of Apulia 2008-2010 Sigma Xi Distinguished Lecturer on Integrative Cancer Biology 1984-1989 Scholar, Leukemia Society of America 1981-1983 Senior Fellow, American Cancer Society, California Division 1978-1980 Fellow, Leukemia Society of America 1976 Fellow, ItalianAmerican Medical Education Foundation SOCIETIES:

American Association for Cancer Research American Association for the Advancement of Science American Association of Immunologists American Society for Cell Biology American Society for Matrix Biology American Society of Biochemistry and Molecular Biology Biophysical Society Society for Developmental Biology

INVITED LECTURES: 2016: Invited Speaker, JSPS Core-to-Core Advanced Research Network Conference, Nara, Japan

Vito Quaranta, MD

2015: Invited Speaker, Workshop 4: Tumor Heterogeneity and the Microenvironment, Mathematical Biosciences Institute, Columbus, OH Invited Speaker, Cellular Dynamics & Models, Cold Spring Harbor Laboratory
 
Cold Spring Harbor, NY
 Invited Speaker, Workshop 5: Targeting Cancer Cell Proliferation and Metabolism Networks, Mathematical Biosciences Institute, Columbus, OH Invited Speaker, EMBO | EMBL Symposium Cellular Heterogeneity: Role of Variability and Noise in Biological Decision-Making, Heidelberg, Germany Invited Speaker, International conference “Chance at the heart of the cell”, Inria, Paris, France Invited Speaker, Workshop: Computational and multiscale mathematical modelling of cancer growth and spread, International Center for Mathematical Sciences, Edinburgh, Scotland 2014: Invited Speaker, AACR National Meeting, San Diego, CA Invited Speaker, Japanese Society for Mathematical Biology/Society for Mathematical Biology, Joint Meeting, Osaka, Japan Invited Speaker, Multi-Scale Imaging for Integrative Cancer 
Biology Workshop, Methodist Research Institute, Houston, TX 2013: Speaker, NCI-ICBP Workshop, Bethesda, MD Invited Speaker, 29th Annual Forbeck Forum on Resistance Mechanisms, Hilton Head, SC 2012: Speaker, International Conference in Systems Medicine, Dublin, Ireland Speaker and Session Chair, Workshop on Mathematical Oncology IV: Integrative Cancer Biology, The Fields Institute, Toronto, Canada 2011: Speaker, Cancer Systems Biology Lecture Series, Stanford, Palo Alto Discussion Panelist, National Brain Tumor Society, Annual Meeting, Philadelphia, PA Invited Speaker, ICIAM 2011, Vancouver, Canada Invited Speaker, International Conference on Mathematical Methods of Cancer Cell Biology, Hiroshima, Japan Keynote Speaker, Kepler Prize Conference on “Managing complexity, reducing perplexity”, Heidelberg, Germany Invited Participant, NIMBioS Investigative Workshop: “Synchronous Activity in Biological Systems”, Knoxville, TN Invited Speaker, Computational Cell Biology Conference, Cold Spring Harbor, NY Invited Speaker, Computational Biology Seminar, Sloan Kettering Institute, New York, NY 2010: Invited Speaker, 7th Biannual Carolina Biophysics Symposium, NCSU, Chapel Hill, NC Invited Speaker, MBI Workshop: Bootcamp in Cancer Modeling, Columbus, OH Invited Speaker, Mathematical Oncology Workshop, Fields Institute, Toronto, Canada 2009:

Vito Quaranta, MD

Invited Speaker, MBI Workshop: Cancer Development, Angiogenesis, Progression and Invasion, Columbus, OH Invited Speaker, Computational Cell Biology Conference, Cold Spring Harbor, NY Invited Participant, The Mathematical Biology of Human Brain Cancer Workshop, Boston, MA Invited Speaker, q-bio Conference on Cellular Information Processing, Santa Fe, NM 2008: Invited Participant, caGRID Roadmap Workshop, Columbus, OH Invited Participant, Integrating and Leveraging the Physical Sciences to Open a New Frontier in Oncology, Arlington, VA Invited Speaker, UCI Symposium on Mathematical Systems Biology, Irvine, CA Invited Speaker, IMA Annual Program Year Workshop, Minneapolis, MN Invited Lecturer, Euroconference on Modelling and Simulation of Cancer Growth and Therapy, Torino, Italy Invited Speaker, European Conference on Mathematical and Theoretical Biology, Edinburgh, Scotland Invited Speaker, Society for Mathematical Biology Conference Mini-symposium, Toronto, Canada Invited Participant, MBI Partners Meeting, Columbus, OH Invited Lecturer, Lectures on Modelling Cancer Growth and Treatment, Estoril, Portugal 2007: Invited Speaker, Joint Meeting of NCI Integrative Cancer Biology Program Centers and NIGMS Systems Biology Centers, Boston, MA Invited Speaker, American Society of Biochemistry and Molecular Biology Annual Meeting, Washington, DC Invited Lecturer, Workshop on Mathematical Modelling and Analysis of Cancer Invasion of Tissues, Dundee, Scotland 2006: Invited Speaker, Mathematical Biology Workshop, Oberwolfach, Germany Invited Participant, NCI Imaging Think Tank, Alexandria, VA Invited Lecturer, Society of Mathematical Biology Annual Meeting, Raleigh, NC Invited Speaker, Symbolic Systems Biology Workshop, Computational Systems Bioinformatics Conference, Stanford, Palo Alto, CA Invited Speaker, Workshop on Cancer Modelling and Therapeutic Innovation, Lyon, France Invited Lecturer, Department of Medicine, Univerity of Illinois, Chicago, IL Invited Speaker, Biomedical Engineering Society Annual Meeting, Chicago, IL Invited Speaker, Frontiers in Cancer Prevention Research, American Association for Cancer Research, Boston, MA Speaker, Joint Meeting of the Integrative Cancer Biology Program and the Mouse Models for Human Cancer Consortium, Bethesda, MD 2005: Speaker, Integrative Cancer Biology Program, NCI, Berkeley, CA Guest Speaker, Data Mining Workshop, Fields Intsitute for Research in Mathematical Sciences, Toronto, Canada 2004: Lecturer, 4th Mediterranean Meeting of Laboratory Medicine, Bari, Italy Speaker, Integrative Cancer Biology Program, NCI, Bethesda, MD

Vito Quaranta, MD

Speaker, Pathology Seminar Series, Vanderbilt University School of Medicine, Nashville, TN Lecturer, 3rd International Symposium on Extracellular Matrix, Rio de Janeiro, Brasil Invited Lecturer, University of New Mexico, Albuquerque, NM Invited Lecturer, Washington University, St. Louis, MO Speaker, Advances in Prostate Cancer Symposium, Tucson, AZ 2003: Invited Lecturer, Symposium on Protein Chemistry, Osaka, Japan Invited Lecturer, University of Southern California Health Science Campus, Los Angeles, CA Speaker and Session Chair, Cell Migration and Invasion, Keystone Symposia on Molecular and Cellular Biology, Beaver Run, Breckenridge, CO 2002: Speaker, Biological Response to the Extracellular Matrix, Keystone Symposia on Molecular and Cellular Biology, Banff, Alberta, Canada Speaker, Basic Research Seminar Series, Childrens Hospital Los Angeles Research Institute, Los Angeles, CA Speaker, Basement Membranes Gordon Conference, Plymouth,New Hampshire Speaker, American Association for Cancer Research, Hilton Head, South Carolina 2001: Speaker, International Conference on Cell Migration in Development and Disease, MaxDelbrück Center, Berlin, Germany Keynote Speaker, Annual Meeting, Italian Society of Clinical Biochemistry, Bari, Italy Speaker, Mammary Gland Biology Gordon Conference, Bristol, RI Invited Lecturer, Swiss Institute for Experimental Cancer Research (ISREC) EpalingesSwitzerland Invited Lecturer, American Red Cross, Bethesda, MD 2000: Speaker, Basement Membranes Gordon Conference, Connecticut Invited Lecturer, Netherlands Cancer Center, Amsterdam, Netherlands Speaker, Epithelial-Stromal Interactions and Tumor Progression, National Cancer Institute Workshop, Bethesda, MD Session Chair, Pancreatic Cancer, National Cancer Institute Meeting, VA 1999: Speaker, International Meeting on Cancer Biology, Kyoto, Japan Speaker, 9th International Symposium on Basement Membranes, Nice, France Invited Lecturer, Dept. of Medicine, University of Florence Medical School, Florence, Italy Invited Lecturer, Department of Pathology, Yale University, New Haven, CT Visiting Professor, University of Arizona Cancer Center, Tucson, AZ Invited Lecturer, Cancer Center, University of California San Francisco, San Francisco, CA 1998: Speaker, Metastasis Research Society, San Diego, CA Invited Lecturer, University of Genoa Medical School, Genoa, Italy 1997: Invited Lecturer, Department of Pathology, Jefferson Medical School, Philadelphia, PA Speaker, Fibronectin Integrins and Related Molecules, Ventura, CA

Vito Quaranta, MD

1995: Speaker, Fibronectin Gordon Conference, Oxnard, CA 1993: Speaker, Fibronectin Gordon Conference, Oxnard, CA Speaker, Integrin Signaling, Chapel Hill, NC 1991: Co-Organizer and Speaker, “Integrins: Cell Adhesion and Transmembrane Communication in Development and Disease”, Keystone Symposia on Molecular and Cellular Biology, Keystone, CO. Publication List http://www.ncbi.nlm.nih.gov/sites/myncbi/vito.quaranta.1/bibliography/40735166/public/?sort=da te&direction=descending

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Harris, L. A., Frick, P. L., Garbett, S. P., Hardeman, K. A., Paudel, B. B., Lopez, C. F., Quaranta, V. and Tyson, D. R. (2016). An unbiased metric of antiproliferative drug effect in vitro. Nat Methods (in press). Frick, P. L., Paudel, B. B., Tyson, D. R. and Quaranta, V. (2015). Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation. J. Cell. Physiol. 230, 1403–1412. Hormuth, D. A., II, Weis, J. A., Barnes, S. L., Miga, M. I., Rericha, E. C., Quaranta, V. and Yankeelov, T. E. (2015). Predicting in vivo glioma growth with the reaction diffusion equation constrained by quantitative magnetic resonance imaging data. Phys. Biol. 12, 046006. Yazlovitskaya, E. M., Tseng, H.-Y., Viquez, O., Tu, T., Mernaugh, G., McKee, K. K., Riggins, K., Quaranta, V., Pathak, A., Carter, B. D., et al. (2015). Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt. Mol Biol Cell 26, 1857–1874. Broussard, J. A., Diggins, N. L., Hummel, S., Georgescu, W., Quaranta, V. and Webb, D. J. (2015). Automated Analysis of Cell-Matrix Adhesions in 2D and 3D Environments. Sci Rep 5, 8124. Yankeelov, T. E., Quaranta, V., Evans, K. J. and Rericha, E. C. (2015). Toward a science of tumor forecasting for clinical oncology. Cancer Res 75, 918–923. Frick, P. L., Paudel, B. B., Tyson, D. R. and Quaranta, V. (2015). Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation. J. Cell. Physiol. 230, 1403–1412. Leander, R., Allen, E. J., Garbett, S. P., Tyson, D. R. and Quaranta, V. (2014). Derivation and experimental comparison of cell-division probability densities. J Theor Biol 359, 129–135. Quaranta, V. and Tyson, D. R. (2013). What lies beneath: looking beyond tumor genetics shows the complexity of signaling networks underlying drug sensitivity. Sci Signal 6, pe32. Tyson, D. R. and Quaranta, V. (2013). Beyond genetics in personalized cancer treatment: assessing dynamics and heterogeneity of tumor responses. Per Med 10, 221–225.

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Udyavar, A. R., Hoeksema, M. D., Clark, J. E., Zou, Y., Tang, Z., Li, Z., Li, M., Chen, H., Statnikov, A., Shyr, Y., …, Massion, PP, and Quaranta V. (2013). Co-expression network analysis identifies Spleen Tyrosine Kinase (SYK) as a candidate oncogenic driver in a subset of small-cell lung cancer. BMC Syst Biol 7 Suppl 5, S1. Yankeelov, T. E., Atuegwu, N., Hormuth, D., Weis, J. A., Barnes, S. L., Miga, M. I., Rericha, E. C. and Quaranta, V. (2013). Clinically relevant modeling of tumor growth and treatment response. Sci Transl Med 5, 187ps9. Tyson, D.R., Garbett, S.P., Frick, P.L. & Quaranta, V. (2012). Fractional proliferation: a method to deconvolve cell population dynamics from single-cell data. Nat Methods 9, 923–928 Gabriel, P., Garbett, S.P., Quaranta, V., Tyson, D.R. & Webb, G.F. (2012). The contribution of age structure to cell population responses to targeted therapeutics. J Theor Biol 311C, 19–27 Rejniak, K.A., Quaranta, V. & Anderson, A.R.A. (2012). Computational investigation of intrinsic and extrinsic mechanisms underlying the formation of carcinoma. Math Med Biol 29, 67–84 Takahashi, K., Mernaugh, R.L., Friedman, D.B., Weller, R., Tsuboi, N., Yamashita, H., Quaranta, V. & Takahashi, T. (2012). Thrombospondin-1 acts as a ligand for CD148 tyrosine phosphatase. Proc Natl Acad Sci USA 109, 1985–1990 Georgescu, W., Wikswo, J.P. & Quaranta, V. (2012). CellAnimation: an open source MATLAB framework for microscopy assays. Bioinformatics 28, 138–139 Hoshino, D., Koshikawa, N., Suzuki, T., Quaranta, V., Weaver, A.M., Seiki, M. & Ichikawa, K. (2012). Establishment and validation of computational model for MT1-MMP dependent ECM degradation and intervention strategies. PLoS Comput Biol 8, e1002479 Hoshino, D., Jourquin, J., Emmons, S.W., Miller, T., Goldgof, M., Costello, K., Tyson, D.R., Brown, B., Lu, Y., Prasad, N.K., Zhang, B., Mills, G.B., Yarbrough, W.G., Quaranta, V., Seiki, M. & Weaver, A.M. (2012). Network Analysis of the Focal Adhesion to Invadopodia Transition Identifies a PI3K-PKCα Invasive Signaling Axis. Sci Signal 5, ra66 Hassanein, M., Weidow, B., Koehler, E., Bakane, N., Garbett, S., Shyr, Y. & Quaranta, V. (2011). Development of high-throughput quantitative assays for glucose uptake in cancer cell lines. Molecular imaging and biology 13, 840–852 Xu, J., Xie, J., Jourquin, J., Colvin, D.C., Does, M.D., Quaranta, V. & Gore, J.C. (2011). Influence of cell cycle phase on apparent diffusion coefficient in synchronized cells detected using temporal diffusion spectroscopy. Magn Reson Med 65, 920–926 Tripathi, M., Potdar, A.A., Yamashita, H., Weidow, B., Cummings, P.T., Kirchhofer, D. & Quaranta, V. (2011). Laminin-332 cleavage by matriptase alters motility parameters of prostate cancer cells. Prostate 71, 184–196 Frick, P.L., Tyson, D.R. & Quaranta, V. (2011). Cancer Cell Cycle and Oncogene Addiction. Encyclopedia of Systems Biology Ocak, S., Yamashita, H., Udyavar, A. R., Miller, A. N., Gonzalez, A. L., Zou, Y., Jiang, A., Yi, Y., Shyr, Y., Estrada, L., Quaranta, V. & Massion, P.P. (2010). DNA copy number aberrations in small-cell lung cancer reveal activation of the focal adhesion pathway. Oncogene 29, 6331–6342 Riggins, K.S., Mernaugh, G., Su, Y., Quaranta, V., Koshikawa, N., Seiki, M., Pozzi, A. & Zent, R. (2010). MT1-MMP-mediated basement membrane remodeling modulates renal development. Exp Cell Res 316, 2993–3005 Gruver, J.S., Potdar, A.A., Jeon, J., Sai, J., Anderson, B., Webb, D., Richmond, A., Quaranta, V., Cummings, P.T. & Chung, C.Y. (2010). Bimodal analysis reveals a general scaling law governing nondirected and chemotactic cell motility. Biophys J 99,

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367–376 Yamashita, H., Tripathi, M., Harris, M.P., Liu, S., Weidow, B., Zent, R. & Quaranta, V. (2010). The role of a recombinant fragment of laminin-332 in integrin alpha3beta1dependent cell binding, spreading and migration. Biomaterials 31, 5110–5121 Yamashita, H., Shang, M., Tripathi, M., Jourquin, J., Georgescu, W., Liu, S., Weidow, B. and Quaranta, V. (2010). Epitope mapping of function-blocking monoclonal antibody CM6 suggests a “weak” integrin binding site on the laminin-332 LG2 domain. J Cell Physiol 223, 541–548. Quaranta, V. & Garbett, S.P. (2010). Not all noise is waste. Nat Methods 7, 269–272 Liu, S., Yamashita, H., Weidow, B., Weaver, A. M. and Quaranta, V. (2010). Laminin332-beta1 integrin interactions negatively regulate invadopodia. J Cell Physiol 223, 134– 142. Jeon, J., Quaranta, V. & Cummings, P.T. (2010). An off-lattice hybrid discretecontinuum model of tumor growth and invasion. Biophys J 98, 37–47 Rejniak, K.A., Wang, S.E., Bryce, N.S., Chang, H., Parvin, B., Jourquin, J., Estrada, L., Gray, J.W., Arteaga, C.L., Weaver, A.M., Quaranta, V. & Anderson, A.R.A. (2010). Linking changes in epithelial morphogenesis to cancer mutations using computational modeling. PLoS Comput Biol 6, (8) Potdar, A.A., Jeon, J., Weaver, A.M., Quaranta, V. & Cummings, P.T. (2010). Human mammary epithelial cells exhibit a bimodal correlated random walk pattern. PLoS ONE 5, e9636 Yamashita, H., Tripathi, M., Jourquin, J., Kam, Y., Liu, S., Weidow, B. & Quaranta, V. (2010). Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal. J Oncol 2010, 1–8 Anderson, A.R.A., Hassanein, M., Branch, K.M., Lu, J., Lobdell, N.A., Maier, J., Basanta, D., Weidow, B., Narasanna, A., Arteaga, C.L., Reynolds, A.B., Quaranta, V., Estrada, L. & Weaver, A.M. (2009). Microenvironmental independence associated with tumor progression. Cancer Res 69, 8797–8806 Guess, C.M. & Quaranta, V. (2009). Defining the role of laminin-332 in carcinoma. Matrix Biol 28, 445–455 Hinow, P., Gerlee, P., McCawley, L.J., Quaranta, V., Ciobanu, M., Wang, S., Graham, J.M., Ayati, B.P., Claridge, J., Swanson, K.R., Loveless, M. & Anderson, A.R.A. (2009). A spatial model of tumor-host interaction: application of chemotherapy. Math Biosci Eng 6, 521–546 Guess, C.M., Lafleur, B.J., Weidow, B.L. & Quaranta, V. (2009). A decreased ratio of laminin-332 beta3 to gamma2 subunit mRNA is associated with poor prognosis in colon cancer. Cancer Epidemiol Biomarkers Prev 18, 1584–1590 Anderson, A.R.A., Rejniak, K.A., Gerlee, P. & Quaranta, V. (2009). Microenvironment driven invasion: a multiscale multimodel investigation. J Math Biol 58, 579–624 Wang, S.E., Xiang, B., Zent, R., Quaranta, V., Pozzi, A. & Arteaga, C.L. (2009). Transforming growth factor beta induces clustering of HER2 and integrins by activating Src-focal adhesion kinase and receptor association to the cytoskeleton. Cancer Res 69, 475–482 Kam, Y., Karperien, A., Weidow, B., Estrada, L., Anderson, A.R. & Quaranta, V. (2009). Nest expansion assay: a cancer systems biology approach to in vitro invasion measurements. BMC Res Notes 2, 130 Quaranta, V., Tyson, D.R., Garbett, S.P., Weidow, B., Harris, M.P. & Georgescu, W. (2009). Trait variability of cancer cells quantified by high-content automated microscopy of single cells. Meth Enzymol 467, 23–57 Kam, Y. & Quaranta, V. (2009). Cadherin-bound beta-catenin feeds into the Wnt pathway upon adherens junctions dissociation: evidence for an intersection between

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beta-catenin pools. PLoS ONE 4, e4580 Potdar, A.A., Lu, J., Jeon, J., Weaver, A.M. & Cummings, P.T. (2009). Bimodal analysis of mammary epithelial cell migration in two dimensions. Ann Biomed Engin 37, 230–245 Tripathi, M., Nandana, S., Yamashita, H., Ganesan, R., Kirchhofer, D. & Quaranta, V. (2008). Laminin-332 is a substrate for hepsin, a protease associated with prostate cancer progression. J Biol Chem 283, 30576–30584 Quaranta, V., Rejniak, K.A., Gerlee, P. & Anderson, A.R.A. (2008). Invasion emerges from cancer cell adaptation to competitive microenvironments: quantitative predictions from multiscale mathematical models. Semin Cancer Biol 18, 338–348 Harris, M.P., Kim, E., Weidow, B., Wikswo, J.P. & Quaranta, V. (2008). Migration of isogenic cell lines quantified by dynamic multivariate analysis of single-cell motility. Cell Adh Migr 2, 127–136 Anderson, A.R.A. & Quaranta, V. (2008). Integrative mathematical oncology. Nat Rev Cancer 8, 227–234 Klees, R.F., Salasznyk, R.M., Ward, D.F., Crone, D.E., Williams, W.A., Harris, M.P., Boskey, A., Quaranta, V. & Plopper, G.E. (2008). Dissection of the osteogenic effects of laminin-332 utilizing specific LG domains: LG3 induces osteogenic differentiation, but not mineralization. Exp Cell Res 314, 763–773 Georgescu, W., Jourquin, J., Estrada, L., Anderson, A.R.A., Quaranta, V. & Wikswo, J.P. (2008). Model-controlled hydrodynamic focusing to generate multiple overlapping gradients of surface-immobilized proteins in microfluidic devices. Lab Chip 8, 238–244 Kam, Y., Guess, C., Estrada, L., Weidow, B. & Quaranta, V. (2008). A novel circular invasion assay mimics in vivo invasive behavior of cancer cell lines and distinguishes single-cell motility in vitro. BMC Cancer 8, 198 Hu, G., Quaranta, V. & Li, D. (2007). Modeling of effects of nutrient gradients on cell proliferation in microfluidic bioreactor. Biotechnol Prog 23, 1347–1354 Anderson, A.R.A., Weaver, A.M., Cummings, P.T. & Quaranta, V. (2006). Tumor morphology and phenotypic evolution driven by selective pressure from the microenvironment. Cell 127, 905–915 Jourquin, J., Yang, N., Kam, Y., Guess, C. and Quaranta, V. (2006). Dispersal of epithelial cancer cell colonies by lysophosphatidic acid (LPA). J Cell Physiol 206, 337– 346. Aumailley, M., Bruckner-Tuderman, L., Carter, W.G., Deutzmann, R., Edgar, D., Ekblom, P., Engel, J., Engvall, E., Hohenester, E., Jones, J.C.R., Kleinman, H.K., Marinkovich, M.P., Martin, G.R., Mayer, U., Meneguzzi, G., Miner, J.H., Miyazaki, K., Patarroyo, M., Paulsson, M., Quaranta, V., Sanes, J.R., Sasaki, T., Sekiguchi, K., Sorokin, L.M., Talts, J.F., Tryggvason, K., Uitto, J., Virtanen, I., Mark, von der, K., Wewer, U.M., Yamada, Y. & Yurchenco, P.D. (2005). A simplified laminin nomenclature. Matrix Biol 24, 326–332 Quaranta, V., Weaver, A.M., Cummings, P.T. & Anderson, A.R.A. (2005). Mathematical modeling of cancer: the future of prognosis and treatment. Clin Chim Acta 357, 173–179 Hintermann, E., Yang, N., O'Sullivan, D., Higgins, J.M.G. & Quaranta, V. (2005). Integrin alpha6beta4-erbB2 complex inhibits haptotaxis by up-regulating E-cadherin cell-cell junctions in keratinocytes. J Biol Chem 280, 8004–8015 Koshikawa, N., Minegishi, T., Sharabi, A., Quaranta, V. & Seiki, M. (2005). Membranetype matrix metalloproteinase-1 (MT1-MMP) is a processing enzyme for human laminin gamma 2 chain. J Biol Chem 280, 88–93 Hintermann, E. & Quaranta, V. (2004). Epithelial cell motility on laminin-5: regulation by matrix assembly, proteolysis, integrins and erbB receptors. Matrix Biol 23, 75–85 Bilban, M., Ghaffari-Tabrizi, N., Hintermann, E., Bauer, S., Molzer, S., Zoratti, C., Malli, R., Sharabi, A., Hiden, U., Graier, W., Knöfler, M., Andreae, F., Wagner, O., Quaranta, V. & Desoye, G. (2004). Kisspeptin-10, a KiSS-1/metastin-derived decapeptide, is a

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physiological invasion inhibitor of primary human trophoblasts. J Cell Sci 117, 1319– 1328 Wang, H., Fu, W., Im, J.H., Zhou, Z., Santoro, S.A., Iyer, V., DiPersio, C.M., Yu, Q.-C., Quaranta, V., Al-Mehdi, A. & Muschel, R.J. (2004). Tumor cell alpha3beta1 integrin and vascular laminin-5 mediate pulmonary arrest and metastasis. J Cell Biol 164, 935–941 Koshikawa, N., Schenk, S., Moeckel, G., Sharabi, A., Miyazaki, K., Gardner, H., Zent, R. & Quaranta, V. (2004). Proteolytic processing of laminin-5 by MT1-MMP in tissues and its effects on epithelial cell morphology. FASEB J 18, 364–366 Giannelli, G., Quaranta, V. & Antonaci, S. (2003). Tissue remodelling in liver diseases. Histol Histopathol 18, 1267–1274 Schenk, S. & Quaranta, V. (2003). Tales from the crypt[ic] sites of the extracellular matrix. Trends Cell Biol 13, 366–375 Quaranta, V. & Giannelli, G. (2003). Cancer invasion: watch your neighbourhood! Tumori 89, 343–348 Hendrix, M.J.C., Seftor, E.A., Kirschmann, D.A., Quaranta, V. & Seftor, R.E.B. (2003). Remodeling of the microenvironment by aggressive melanoma tumor cells. Ann N Y Acad Sci 995, 151–161 Pirilä, E., Sharabi, A., Salo, T., Quaranta, V., Tu, H., Heljasvaara, R., Koshikawa, N., Sorsa, T. & Maisi, P. (2003). Matrix metalloproteinases process the laminin-5 gamma 2chain and regulate epithelial cell migration. Biochem Biophys Res Commun 303, 1012– 1017 Schenk, S., Hintermann, E., Bilban, M., Koshikawa, N., Hojilla, C., Khokha, R. & Quaranta, V. (2003). Binding to EGF receptor of a laminin-5 EGF-like fragment liberated during MMP-dependent mammary gland involution. J Cell Biol 161, 197–209 Gao, C., Mao, S., Ronca, F., Zhuang, S., Quaranta, V., Wirsching, P. & Janda, K.D. (2003). De novo identification of tumor-specific internalizing human antibody-receptor pairs by phage-display methods. J Immunol Methods 274, 185–197 Quaranta, V. (2002). Motility cues in the tumor microenvironment. Differentiation 70, 590–598 Hintermann, E., Haake, S.K., Christen, U., Sharabi, A. & Quaranta, V. (2002). Discrete proteolysis of focal contact and adherens junction components in Porphyromonas gingivalis-infected oral keratinocytes: a strategy for cell adhesion and migration disabling. Infect Immun 70, 5846–5856 Bilban, M., Buehler, L.K., Head, S., Desoye, G. & Quaranta, V. (2002). Defining signal thresholds in DNA microarrays: exemplary application for invasive cancer. BMC Genomics 3, 19 Faccio, R., Grano, M., Colucci, S., Villa, A., Giannelli, G., Quaranta, V. & Zallone, A. (2002). Localization and possible role of two different alpha v beta 3 integrin conformations in resting and resorbing osteoclasts. J Cell Sci 115, 2919–2929 Bilban, M., Buehler, L.K., Head, S., Desoye, G. & Quaranta, V. (2002). Normalizing DNA microarray data. Curr Issues Mol Biol 4, 57–64 Zent, R., Bush, K.T., Pohl, M.L., Quaranta, V., Koshikawa, N., Wang, Z., Kreidberg, J.A., Sakurai, H., Stuart, R.O. & Nigám, S.K. (2001). Involvement of laminin binding integrins and laminin-5 in branching morphogenesis of the ureteric bud during kidney development. Dev Biol 238, 289–302 Seftor, R.E., Seftor, E.A., Koshikawa, N., Meltzer, P.S., Gardner, L.M., Bilban, M., Stetler-Stevenson, W.G., Quaranta, V. & Hendrix, M.J. (2001). Cooperative interactions of laminin 5 gamma2 chain, matrix metalloproteinase-2, and membrane type-1matrix/metalloproteinase are required for mimicry of embryonic vasculogenesis by aggressive melanoma. Cancer Res 61, 6322–6327 Shang, M., Koshikawa, N., Schenk, S. and Quaranta, V. (2001). The LG3 module of

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78. 79.

80. 81.

82. 83. 84. 85. 86. 87. 88. 89. 90. 91. 92. 93.

laminin-5 harbors a binding site for integrin alpha3beta1 that promotes cell adhesion, spreading, and migration. J Biol Chem 276, 33045–33053. Hintermann, E., Bilban, M., Sharabi, A. & Quaranta, V. (2001). Inhibitory role of alpha 6 beta 4-associated erbB-2 and phosphoinositide 3-kinase in keratinocyte haptotactic migration dependent on alpha 3 beta 1 integrin. J Cell Biol 153, 465–478 Giannelli, G., Bergamini, C., Fransvea, E., Marinosci, F., Quaranta, V. & Antonaci, S. (2001). Human hepatocellular carcinoma (HCC) cells require both alpha3beta1 integrin and matrix metalloproteinases activity for migration and invasion. Lab Invest 81, 613– 627 Kiosses, W.B., Hahn, K.M., Giannelli, G. & Quaranta, V. (2001). Characterization of morphological and cytoskeletal changes in MCF10A breast epithelial cells plated on laminin-5: comparison with breast cancer cell line MCF7. Cell Commun Adhes 8, 29–44 Cirulli, V., Beattie, G.M., Klier, G., Ellisman, M., Ricordi, C., Quaranta, V., Frasier, F., Ishii, J.K., Hayek, A. & Salomon, D.R. (2000). Expression and function of alpha(v)beta(3) and alpha(v)beta(5) integrins in the developing pancreas: roles in the adhesion and migration of putative endocrine progenitor cells. J Cell Biol 150, 1445–1460 Plopper, G.E., Huff, J.L., Rust, W.L., Schwartz, M.A. & Quaranta, V. (2000). Antibodyinduced activation of beta1 integrin receptors stimulates cAMP-dependent migration of breast cells on laminin-5. Mol Cell Biol Res Commun 4, 129–135 Quaranta, V. (2000). Cell migration through extracellular matrix: membrane-type metalloproteinases make the way. J Cell Biol 149, 1167–1170 Koshikawa, N., Giannelli, G., Cirulli, V., Miyazaki, K. & Quaranta, V. (2000). Role of cell surface metalloprotease MT1-MMP in epithelial cell migration over laminin-5. J Cell Biol 148, 615–624 Bilban, M., Head, S., Desoye, G. & Quaranta, V. (2000). DNA microarrays: a novel approach to investigate genomics in trophoblast invasion--a review. Placenta 21 Suppl A, S99–105 Romano, T.A., Ridgway, S.H., Felten, D.L. & Quaranta, V. (1999). Molecular cloning and characterization of CD4 in an aquatic mammal, the white whale Delphinapterus leucas. Immunogenetics 49, 376–383 Giannelli, G., Pozzi, A., Stetler-Stevenson, W.G., Gardner, H.A. & Quaranta, V. (1999). Expression of matrix metalloprotease-2-cleaved laminin-5 in breast remodeling stimulated by sex steroids. Am J Pathol 154, 1193–1201 Vivinus-Nebot, M., Ticchioni, M., Mary, F., Hofman, P., Quaranta, V., Rousselle, P. & Bernard, A. (1999). Laminin 5 in the human thymus: control of T cell proliferation via alpha6beta4 integrins. J Cell Biol 144, 563–574 Mullen, L.M., Richards, D.W. & Quaranta, V. (1999). Evidence that laminin-5 is a component of the tooth surface internal basal lamina, supporting epithelial cell adhesion. J Periodont Res 34, 16–24 Fitchmun, M.I., Falk-Marzillier, J., Marshall, E., Cruz, G., Jones, J.C. & Quaranta, V. (1998). Mode of adsorption and orientation of an extracellular matrix protein affect its cell-adhesion-promoting activity. Anal Biochem 265, 1–7 Falk-Marzillier, J., Domanico, S.Z., Pelletier, A., Mullen, L. & Quaranta, V. (1998). Characterization of a tight molecular complex between integrin alpha 6 beta 4 and laminin-5 extracellular matrix. Biochem Biophys Res Commun 251, 49–55 Plopper, G.E., Domanico, S.Z., Cirulli, V., Kiosses, W.B. & Quaranta, V. (1998). Migration of breast epithelial cells on Laminin-5: differential role of integrins in normal and transformed cell types. Breast Cancer Res Treat 51, 57–69 Faccio, R., Grano, M., Colucci, S., Zallone, A.Z., Quaranta, V. & Pelletier, A.J. (1998). Activation of alphav beta3 integrin on human osteoclast-like cells stimulates adhesion and migration in response to osteopontin. Biochem Biophys Res Commun 249, 522–525

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Wong, N.C., Mueller, B.M., Barbas, C.F., Ruminski, P., Quaranta, V., Lin, E.C. & Smith, J.W. (1998). Alphav integrins mediate adhesion and migration of breast carcinoma cell lines. Clin Exp Metastasis 16, 50–61 Domanico, S.Z., Pelletier, A.J., Havran, W.L. & Quaranta, V. (1997). Integrin alpha 6A beta 1 induces CD81-dependent cell motility without engaging the extracellular matrix migration substrate. Mol Biol Cell 8, 2253–2265 Giannelli, G., Falk-Marzillier, J., Schiraldi, O., Stetler-Stevenson, W.G. & Quaranta, V. (1997). Induction of cell migration by matrix metalloprotease-2 cleavage of laminin-5. Science 277, 225–228 Quaranta, V. & Plopper, G.E. (1997). Integrins and laminins in tissue remodeling. Kidney Int 51, 1441–1446 Tamura, R.N., Oda, D., Quaranta, V., Plopper, G., Lambert, R., Glaser, S. & Jones, J.C. (1997). Coating of titanium alloy with soluble laminin-5 promotes cell attachment and hemidesmosome assembly in gingival epithelial cells: potential application to dental implants. J Periodont Res 32, 287–294 Wagner, S., Tagaya, M., Koziol, J.A., Quaranta, V. & del Zoppo, G.J. (1997). Rapid disruption of an astrocyte interaction with the extracellular matrix mediated by integrin alpha 6 beta 4 during focal cerebral ischemia/reperfusion. Stroke 28, 858–865 Baker, S.E., DiPasquale, A.P., Stock, E.L., Quaranta, V., Fitchmun, M. & Jones, J.C. (1996). Morphogenetic effects of soluble laminin-5 on cultured epithelial cells and tissue explants. Exp Cell Res 228, 262–270 Baker, S.E., Hopkinson, S.B., Fitchmun, M., Andreason, G.L., Frasier, F., Plopper, G., Quaranta, V. & Jones, J.C. (1996). Laminin-5 and hemidesmosomes: role of the alpha 3 chain subunit in hemidesmosome stability and assembly. J Cell Sci 109 ( Pt 10), 2509– 2520 Plopper, G., Falk-Marzillier, J., Glaser, S., Fitchmun, M., Giannelli, G., Romano, T., Jones, J.C. & Quaranta, V. (1996). Changes in expression of monoclonal antibody epitopes on laminin-5r induced by cell contact. J Cell Sci 109 ( Pt 7), 1965–1973 Colucci, S., Giannelli, G., Grano, M., Faccio, R., Quaranta, V. & Zallone, A.Z. (1996). Human osteoclast-like cells selectively recognize laminin isoforms, an event that induces migration and activates Ca2+ mediated signals. J Cell Sci 109 ( Pt 6), 1527–1535 Giannelli, G., Brassard, J., Foti, C., Stetler-Stevenson, W.G., Falk-Marzillier, J., Zambonin-Zallone, A., Schiraldi, O. & Quaranta, V. (1996). Altered expression of basement membrane proteins and their integrin receptors in lichen planus: possible pathogenetic role of gelatinases A and B. Lab Invest 74, 1091–1104 Zigrino, P., Gaietta, G., Zambonin-Zallone, A., Pelletier, A.J. & Quaranta, V. (1996). Evidence for a K+ channel requirement in spreading of rat basophilic leukemia cells on fibronectin-coated surfaces. Biochem Biophys Res Commun 221, 51–58 Tennenbaum, T., Belanger, A.J., Quaranta, V. & Yuspa, S.H. (1996). Differential regulation of integrins and extracellular matrix binding in epidermal differentiation and squamous tumor progression. J Investig Dermatol Symp Proc 1, 157–161 Pelletier, A.J., Kunicki, T. & Quaranta, V. (1996). Activation of the integrin alpha v beta 3 involves a discrete cation-binding site that regulates conformation. J Biol Chem 271, 1364–1370 Hormia, M., Falk-Marzillier, J., Plopper, G., Tamura, R.N., Jones, J.C. & Quaranta, V. (1995). Rapid spreading and mature hemidesmosome formation in HaCaT keratinocytes induced by incubation with soluble laminin-5r. J Invest Dermatol 105, 557–561 Pelletier, A.J., Kunicki, T., Ruggeri, Z.M. & Quaranta, V. (1995). The activation state of the integrin alpha IIb beta 3 affects outside-in signals leading to cell spreading and focal adhesion kinase phosphorylation. J Biol Chem 270, 18133–18140 Tennenbaum, T., Belanger, A.J., Glick, A.B., Tamura, R., Quaranta, V. & Yuspa, S.H.

Vito Quaranta, MD

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115.

116.

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120. 121. 122. 123. 124. 125.

(1995). A splice variant of alpha 6 integrin is associated with malignant conversion in mouse skin tumorigenesis. Proc Natl Acad Sci USA 92, 7041–7045 Collo G, Domanico, S.Z., Klier, G. & Quaranta, V. (1995). Gradient of integrin alpha 6A distribution in the myocardium during early heart development. Cell Adhes Commun 3, 101–113 Hemler ME, Quaranta, V., Starr L, Bodorova J, Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein RL, Springer TA, Tedder TF & Todd RF (1995). CD49f cluster report. Leukocyte Typing V 1619–1620 Gaietta, G., Redelmeier, T.E., Jackson, M.R., Tamura, R.N. & Quaranta, V. (1994). Quantitative measurement of alpha 6 beta 1 and alpha 6 beta 4 integrin internalization under cross-linking conditions: a possible role for alpha 6 cytoplasmic domains. J Cell Sci 107 ( Pt 12), 3339–3349 Giannelli, G., Savoia, P., Schiraldi, O., Lospalluti, M., De Luca, M., Marchisio, P.C. & Quaranta, V. (1994). Psoriatic lesions in patients with chronic liver disease are distinct from psoriasis vulgaris lesions, as judged on basis of integrin adhesion receptors. Hepatology 20, 56–65 Feltri, M.L., Scherer, S.S., Nemni, R., Kamholz, J., Vogelbacker, H., Scott, M.O., Canal, N., Quaranta, V. & Wrabetz, L. (1994). Beta 4 integrin expression in myelinating Schwann cells is polarized, developmentally regulated and axonally dependent. Development 120, 1287–1301 Lehmann, M., Rabenandrasana, C., Tamura, R., Lissitzky, J.C., Quaranta, V., Pichon, J. & Marvaldi, J. (1994). A monoclonal antibody inhibits adhesion to fibronectin and vitronectin of a colon carcinoma cell line and recognizes the integrins alpha v beta 3, alpha v beta 5, and alpha v beta 6. Cancer Res 54, 2102–2107 O'Toole, T.E., Katagiri, Y., Faull, R.J., Peter, K., Tamura, R., Quaranta, V., Loftus, J.C., Shattil, S.J. & Ginsberg, M.H. (1994). Integrin cytoplasmic domains mediate inside-out signal transduction. J Cell Biol 124, 1047–1059 Quaranta, V., Collo G, Rozzo C, Starr L, Gaietta G, &Tamura RN (1994). The integrin alpha6beta4 in epithelial and carcinoma cells. Integrins: The Biological Problems 147– 176, Takada, Y. ed. Quaranta, V., Tamura RN, Collo G, Cooper HM, Hormia M, Rozzo C, Gaietta G, & Starr L, (1994). Distinctive functions of alpha6beta4 and other integrins in epithelial cells. Integrins: Molecular and Biological Responses to the Extracellular Matrix 141–161, Cheresh D & Mecham RP eds. Collo G, Starr, L. & Quaranta, V. (1993). A new isoform of the laminin receptor integrin alpha 7 beta 1 is developmentally regulated in skeletal muscle. J Biol Chem 268, 19019– 19024 Quaranta, V. (1993). Integrin expression and epithelial cell differentiation. Cell Adhesion Molecules 13–27, Hemler ME & Mihich E, eds. Koukoulis, G.K., Virtanen, I., Moll, R., Quaranta, V. & Gould, V.E. (1993). Immunolocalization of integrins in the normal and neoplastic colonic epithelium. Virchows Arch, B, Cell Pathol 63, 373–383 Korhonen, M., Laitinen, L., Ylanne, J., Koukoulis, G.K., Quaranta, V., Juusela, H., Gould, V.E. & Virtanen, I. (1992). Integrin distributions in renal cell carcinomas of various grades of malignancy. Am J Pathol 141, 1161–1171 Starr, L. & Quaranta, V. (1992). An efficient and reliable method for cloning PCRamplification products: a survey of point mutations in integrin cDNA. BioTechniques 13, 612–618 Romano, T.A., Ridgway, S.H. & Quaranta, V. (1992). MHC class II molecules and immunoglobulins on peripheral blood lymphocytes of the bottlenosed dolphin, Tursiops truncatus. J Exp Zool 263, 96–104

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126. Hormia, M., Virtanen, I. & Quaranta, V. (1992). Immunolocalization of integrin alpha 6 beta 4 in mouse junctional epithelium suggests an anchoring function to both the internal and the external basal lamina. J Dent Res 71, 1503–1508 127. Kurpakus, M.A., Quaranta, V. & Jones, J.C. (1991). Surface relocation of alpha 6 beta 4 integrins and assembly of hemidesmosomes in an in vitro model of wound healing. J Cell Biol 115, 1737–1750 128. Tamura, R.N., Cooper, H.M., Collo G & Quaranta, V. (1991). Cell type-specific integrin variants with alternative alpha chain cytoplasmic domains. Proc Natl Acad Sci USA 88, 10183–10187 129. Cooper, H.M., Tamura, R.N. & Quaranta, V. (1991). The major laminin receptor of mouse embryonic stem cells is a novel isoform of the alpha 6 beta 1 integrin. J Cell Biol 115, 843–850 130. Koukoulis, G.K., Virtanen, I., Korhonen, M., Laitinen, L., Quaranta, V. & Gould, V.E. (1991). Immunohistochemical localization of integrins in the normal, hyperplastic, and neoplastic breast. Correlations with their functions as receptors and cell adhesion molecules. Am J Pathol 139, 787–799 131. Korhonen, M., Ylanne, J., Laitinen, L., Cooper, H.M., Quaranta, V. & Virtanen, I. (1991). Distribution of the alpha 1-alpha 6 integrin subunits in human developing and term placenta. Lab Invest 65, 347–356 132. Quaranta, V. & Jones, J.C. (1991). The internal affairs of an integrin. Trends Cell Biol 1, 2–4 133. Jones, J.C., Kurpakus, M.A., Cooper, H.M. & Quaranta, V. (1991). A function for the integrin alpha 6 beta 4 in the hemidesmosome. Cell Regul 2, 427–438 134. de Curtis, I., Quaranta, V., Tamura, R.N. & Reichardt, L.F. (1991). Laminin receptors in the retina: sequence analysis of the chick integrin alpha 6 subunit. Evidence for transcriptional and posttranslational regulation. J Cell Biol 113, 405–416 135. Lawrance SK, Quaranta, V., & Srivastava R, (1991). Molecular bases of MHC diversity. Current Concepts in Immunogenetics 39–64, Ram BP & Tyle P eds. 136. Lotteau, V., Teyton, L., Peleraux, A., Nilsson, T., Karlsson, L., Schmid, S.L., Quaranta, V. & Peterson, P.A. (1990). Intracellular transport of class II MHC molecules directed by invariant chain. Nature 348, 600–605 137. Quaranta, V. (1990). Epithelial integrins. Cell Differ Dev 32, 361–365 138. Tamura, R.N., Rozzo, C., Starr, L., Chambers, J., Reichardt, L.F., Cooper, H.M. & Quaranta, V. (1990). Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4. J Cell Biol 111, 1593–1604 139. De Luca, M., Tamura, R.N., Kajiji, S., Bondanza, S., Rossino, P., Cancedda, R., Marchisio, P.C. & Quaranta, V. (1990). Polarized integrin mediates human keratinocyte adhesion to basal lamina. Proc Natl Acad Sci USA 87, 6888–6892 140. Sheppard, D., Rozzo, C., Starr, L., Quaranta, V., Erle, D.J. & Pytela, R. (1990). Complete amino acid sequence of a novel integrin beta subunit (beta 6) identified in epithelial cells using the polymerase chain reaction. J Biol Chem 265, 11502–11507 141. Lawrance SK, Karlsson L, Price J, & Quaranta, V. (1990). T cell recognition of Major Histocompatibility Complex antigens in HLA class II transgenic mice. Transgenic Mice and Mutants in MHC Research 247–256, Ron Y, Sprent J, Peterson PA, David C & Egorov I. eds. 142. Spiro, R.C. & Quaranta, V. (1989). The invariant chain is a phosphorylated subunit of class II molecules. J Immunol 143, 2589–2594 143. Lawrance, S.K., Karlsson, L., Price J, Quaranta, V., Ron Y, Sprent J & Peterson, P.A. (1989). Transgenic HLA-DR alpha faithfully reconstitutes IE-controlled immune functions and induces cross-tolerance to E alpha in E alpha 0 mutant mice. Cell 58, 583–594 144. Cheresh, D.A., Smith, J.W., Cooper, H.M. & Quaranta, V. (1989). A novel vitronectin

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145. 146.

147. 148. 149.

150.

151. 152.

153. 154.

155. 156. 157. 158. 159.

receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells. Cell 57, 59–69 Kajiji, S., Tamura, R.N. & Quaranta, V. (1989). A novel integrin (alpha E beta 4) from human epithelial cells suggests a fourth family of integrin adhesion receptors. EMBO J 8, 673–680 Sundblad, G., Kajiji, S., Quaranta, V., Freeze, H.H. & Varki, A. (1988). Sulfated N-linked oligosaccharides in mammalian cells. III. Characterization of a pancreatic carcinoma cell surface glycoprotein with N- and O-sulfate esters on asparagine-linked glycans. J Biol Chem 263, 8897–8903 O'Sullivan, D.M., Noonan, D. & Quaranta, V. (1987). Four Ia invariant chain forms derive from a single gene by alternate splicing and alternate initiation of transcription/translation. J Exp Med 166, 444–460 Ball, S.S., Quaranta, V., Shadravan, F. & Walford, R.L. (1987). An ELISA for detection of DNA-bound carcinogen using a monoclonal antibody to N-acetoxy-2acetylaminofluorene-modified DNA. J Immunol Methods 98, 195–200 Bono, C., Pereira, M.B., O'Sullivan, D.M., Quaranta, V. & Schwartz, B.D. (1987). The HLA-class II-associated chondroitin sulfate proteoglycan expressed by class II positive T and monocyte-like cell lines is larger than that expressed by EBV-transformed Blymphoblastoid cell lines. Hum Immunol 18, 315–330 Posillico, J.T., Wilson, R.E., Srikanta, S.S., Eisenbarth, G.S., Letarte, M., Quackenbush, E.J., Quaranta, V., Kajaji, S. & Brown, E.M. (1987). Monoclonal antibody-mediated modulation of parathyroid hormone secretion by dispersed parathyroid cells. Arch Surg 122, 436–442 Kajiji, S.M., Davceva, B. & Quaranta, V. (1987). Six monoclonal antibodies to human pancreatic cancer antigens. Cancer Res 47, 1367–1376 Posillico, J.T., Srikanta, S., Eisenbarth, G., Quaranta, V., Kajiji, S. & Brown, E.M. (1987). Binding of monoclonal antibody (4F2) to its cell surface antigen on dispersed adenomatous parathyroid cells raises cytosolic calcium and inhibits parathyroid hormone secretion. J Clin Endocrinol Metab 64, 43–50 Giacoletto, K.S., Sant, A.J., Bono, C., Gorka, J., O'Sullivan, D.M., Quaranta, V. & Schwartz, B.D. (1986). The human invariant chain is the core protein of the human class II-associated proteoglycan. J Exp Med 164, 1422–1439 O'Sullivan, D.M., Larhammar, D., Wilson, M.C., Peterson, P.A. & Quaranta, V. (1986). Structure of the human Ia-associated invariant (gamma)-chain gene: identification of 5' sequences shared with major histocompatibility complex class II genes. Proc Natl Acad Sci USA 83, 4484–4488 Harper, J.R., Quaranta, V. & Reisfeld, R.A. (1986). Ammonium chloride interferes with a distinct step in the biosynthesis and cell surface expression of human melanoma-type chondroitin sulfate proteoglycan. J Biol Chem 261, 3600–3606 Wong, C.W., Quaranta, V. & Glenner, G.G. (1985). Neuritic plaques and cerebrovascular amyloid in Alzheimer disease are antigenically related. Proc Natl Acad Sci USA 82, 8729–8732 Nowell, J. & Quaranta, V. (1985). Chloroquine affects biosynthesis of Ia molecules by inhibiting dissociation of invariant (gamma) chains from alpha-beta dimers in B cells. J Exp Med 162, 1371–1376 Harper JR, Reisfeld RA, & Quaranta, V. (1985). Biosynthesis and intracellular transport of proteoglycan core protein by human melanoma cells: Involvement of low-pH mechanisms. Extracellular Matrix: Structure and Function 367–372, Reddi AH, ed. Quaranta, V., Majdic, O., Stingl, G., Liszka, K., Honigsmann, H. & Knapp, W. (1984). A human Ia cytoplasmic determinant located on multiple forms of invariant chain (gamma, gamma 2, gamma 3). J Immunol 132, 1900–1905

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160. Glenner, G.G., Wong, C.W., Quaranta, V. & Eanes, E.D. (1984). The amyloid deposits in Alzheimer's disease: their nature and pathogenesis. Appl Pathol 2, 357–369 161. O'Sullivan D, & Quaranta, V. (1984). Three distinct forms of gamma (invariant) chain associate with Ia antigens. Disease Markers 2, 169–173 162. Quaranta, V. (1983). HLA complex and immune response in health and disease. Clin Physiol Biochem 1, 173–178 163. Quaranta, V., Zanetti, M. & Reisfeld, R.A. (1982). A recurrent idiotype on monoclonal anti-human Ia antibodies. J Exp Med 156, 1551–1556 164. Ng, A.K., Indiveri, F., Russo C, Quaranta, V., Pellegrino, M.A. & Ferrone S (1981). Characterization of human null cells isolated from peripheral lymphocytes by a simultaneous double-rosetting procedure. Scand J Immunol 14, 225–231 165. Wilson, B.S., Glassy, M.C., Quaranta, V., Ng, A.K. & Ferrone S (1981). Effect of tunicamycin on the assembly and antigenicity of HLA antigens: analysis with monoclonal antibodies. Scand J Immunol 14, 201–205 166. Indiveri, F., Russo C, Quaranta, V., Pellegrino, M.A. & Ferrone S (1981). HLA-DR antigens and mitogenic factors released by PWM-stimulated T lymphocytes share the framework determinant recognized by the monoclonal antibody Q5/13. Cell Immunol 62, 406–411 167. Natali, P.G., De Martino, C., Marcellini, M., Quaranta, V. & Ferrone S (1981). Expression of Ia-like antigens on the vasculature of human kidney. Clin Immunol Immunopathol 20, 11–20 168. Natali, P.G., Cordiali-Fei, P., Cavaliere, R., Di Filippo, F., Quaranta, V., Pellegrino, M.A. & Ferrone S (1981). Ia-like antigens on freshly explanted human melanoma. Clin Immunol Immunopathol 19, 250–259 169. Wilson, B.S., Indiveri, F., Quaranta, V., Pellegrino, M.A. & Ferrone S (1981). Level of Ialike antigens on human B and T lymphocytes. Transplant Proc 13, 1033–1034 170. Natali, P.G., Quaranta, V., Nicotra, M.R., Apollonj, C., Pellegrino, M.A. & Ferrone S (1981). Tissue distribution of Ia-like antigens in different species: analysis with monoclonal antibodies. Transplant Proc 13, 1026–1029 171. Quaranta, V., Pellegrino, M.A. & Ferrone S (1981). Serologic and immunochemical characterization of the specificity of four monoclonal antibodies to distinct antigenic determinants expressed on subpopulations of human Ia-like antigens. J Immunol 126, 548–552 172. Curry, R.A., Quaranta, V., Pellegrino, M.A. & Ferrone S (1981). Lysis of cultured human melanoma M10 cells by polyclonal xenoantibodies to melanoma-associated antigens. Cancer Res 41, 463–466 173. Natali, P.G., De Martino, C., Quaranta, V., Nicotra, M.R., Frezza, F., Pellegrino, M.A. & Ferrone S (1981). Expression of Ia-like antigens in normal human nonlymphoid tissues. Transplantation 31, 75–78 174. Quaranta, V., Russo C., Pellegrino M.A., & Ferrone S. (1981). Fine specificity of monoclonal xenoantibodies to distinct immunological domains of human Ia-like antigens. Monoclonal antibodies and T-cell hybridomas 84–92, Hammerling GJ, Hammerling U & Kearney JF, eds. 175. Russo C, Indiveri, F., Quaranta, V., Molinaro, G.A., Pellegrino, M.A. & Ferrone S (1981). Stimulation of human T lymphocytes by PHA-activated autologous T lymphocytes: analysis of the role of Ia-like antigens with monoclonal antibodies. Immunogenetics 12, 267–274 176. Quaranta, V., Pellegrino, M.A. & Ferrone S. (1981). The monoclonal xenoantibody Q6/64 recognizes a determinant expressed by certain gene products of the A and B loci of the HLA region. Immunogenetics 14, 403–413 177. Walker L.E., Quaranta, V., Ferrone S., Pellegrino M.A., & Reisfeld R.A. (1981).

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178.

179. 180. 181. 182.

183. 184. 185. 186. 187.

188. 189.

190. 191.

192.

Structural characterization of human Ia-like antigens by peptide mapping. In: Immunobiology of the Major Histocompatibility Complex 147–154, Zaleski MB, Abeyounis CJ & Kano K, eds. Quaranta, V., Glassy M.C., Indiveri F., Natali P.G., Ng A.K., Pellegrino M.A., Russo C., Wilson B.S., &Ferrone S. in: Zaleski MB, Abeyounis CJ & Kano K (1981). Analysis of human Ia-like antigens with monoclonal antibodies. In: Immunobiology of the Major Histocompatibility Complex 147–154, Zaleski MB, Abeyounis CJ & Kano K, eds. Wilson B.S., Ng A.K., Quaranta, V., Ferrone S., Reisfeld R.A. & Ferrone S. (1981). HLA polyclonal and monoclonal xenoantibodies. In: Current Trends in Histocompatibility 307– 345 Quaranta, V., Pellegrino, M.A., Callahan, G.N. & Ferrone S (1981). Cross-reactivity between human and murine lymphocyte antigens. IV. Reactivity of H-2 alloantisera with HLA-A, B antigens. Immunogenetics 13, 311–317 Natali, P.G., De Martino, C., Quaranta, V., Bigotti, A., Pellegrino, M.A. & Ferrone S (1981). Changes in Ia-like antigen expression on malignant human cells. Immunogenetics 12, 409–413 Quaranta, V., Walker, L.E., Ruberto, G., Pellegrino, M.A. & Ferrone S (1981). The free and the beta 2-microglobulin-associated heavy chains of HLA-A, B alloantigens share the antigenic determinant recognized by the monoclonal antibody Q1/28. Immunogenetics 13, 285–295 Quaranta, V., Tanigaki, N. & Ferrone S (1981). Distribution of antigenic determinants recognized by three monoclonal antibodies (Q2/70, Q5/6 and Q5/13) on human Ia-like alloantigens and on their subunits. Immunogenetics 12, 175–182 Indiveri, F., Wilson, B.S., Russo C, Quaranta, V., Pellegrino, M.A. & Ferrone S (1980). Ia-like antigens on human T lymphocytes: relationship to other surface markers, role in mixed lymphocyte reactions, and structural profile. J Immunol 125, 2673–2678 Fitchen, J.H., Ferrone S, Quaranta, V., Molinaro, G.A. & Cline, M.J. (1980). Monoclonal antibodies to HLA-A,B, and Ia-like antigens inhibit colony formation by human myeloid progenitor cells. J Immunol 125, 2004–2008 Quaranta, V., Walker, L.E., Pellegrino, M.A. & Ferrone S (1980). Purification of immunologically functional subsets of human Ia-like antigens on a monoclonal antibody (Q5/13) immunoadsorbent. J Immunol 125, 1421–1425 Quaranta, V., Indiveri, F., Glassy, M.C., Ng, A., Russo C, Molinaro, G.A., Pellegrino, M.A. & Ferrone S (1980). Serological, functional, and immunochemical characterization of a monoclonal antibody (MoAb Q2/70) to human Ia-like antigens. Hum Immunol 1, 211–223 Russo C, Indiveri, F., Quaranta, V., Molinaro, G.A., Pellegrino, M.A. & Ferrone S (1980). Use of monoclonal antibodies to investigate immunologic cross-reactivity of histocompatibility antigens from various animal species. Transplant Proc 12, 376–379 Ng, A.K., Indiveri, F., Pellegrino, M.A., Molinaro, G.A., Quaranta, V. & Ferrone S (1980). Natural cytotoxicity and antibody-dependent cellular cytotoxicity of human lymphocytes depleted of HLA-DR bearing cells with monoclonal HLA-DR antibodies. J Immunol 124, 2336–2340 Wilson, B.S., Indiveri, F., Molinaro, G.A., Quaranta, V. & Ferrone S (1980). Characterization of DR antigens on cultured melanoma cells by using monoclonal antibodies. Transplant Proc 12, 125–129 Indiveri, F., Ng, A.K., Russo C, Quaranta, V., Pellegrino, M.A. & Ferrone S (1980). Isolation of Ia-like antigen-bearing cells from human peripheral lymphocytes through the use of a monoclonal antibody to framework determinants of Ia-like antigens. J Immunol Methods 39, 343–354 Quaranta, V., Imai K., Molinaro G.A., & Ferrone S. (1980). Manufacture of monoclonal

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193. 194. 195. 196. 197. 198. 199.

200.

201.

antibodies to human melanoma associated and histocompatibility antigens. Immunoassays: Clinical laboratory techniques for the 1980's 301–324, , Nakamura RM, Dito WR & Tucker I, eds. Russo C, Quaranta, V., Indiveri, F., Pellegrino, M.A. & Ferrone S (1980). Effect of polyclonal and monoclonal HLA-DR xenoantibodies on xenogeneic mixed lymphocyte reactions. Immunogenetics 11, 413–416 Indiveri, F., Pellegrino, M.A., Quaranta, V., Molinaro, G.A. & Ferrone S (1980). Monoclonal and polyclonal xenoantibodies to the HLA-A, B, C antigenic molecular complex do not affect the functional activity of C3 receptors. Immunobiology 158, 17–21 Molinaro, G.A., Quaranta, V. & Ferrone S (1979). Characterization of the specificity of DR monoclonal antibodies. Transplant Proc 11, 1748–1750 Glassy, M.C., Pellegrino, M.A., Quaranta, V., Molinaro, G.A. & Ferrone S (1979). Lack of DR antigens on human B-lymphocyte-mouse fibroblast hybrids that express HLA-A,B antigens. Transplant Proc 11, 1719–1721 Curry, R.A., Quaranta, V., Pellegrino, M.A. & Ferrone S (1979). Serologically detectable human melanoma-associated antigens are not genetically linked to HLA-A and B antigens. J Immunol 122, 2630–2632 McCabe, R.P., Quaranta, V., Frugis, L., Ferrone S & Reisfeld, R.A. (1979). A radioimmunometric antibody-binding assay for evaluation of xenoantisera to melanomaassociated antigens. J Natl Cancer Inst 62, 455–463 Indiveri, F., Pellegrino, M.A., Molinaro, G.A., Quaranta, V. & Ferrone S (1979). Rosetting of human T lymphocytes with goat red blood cells: effect of treatment with 2aminoethylisothiouronium bromide (AET) and comparison with AET treated sheep red blood cells. J Immunol Methods 30, 317–328 Curry RA, Quaranta, V., Wilson BS, McCabe RP, Natali PG, Pellegrino MA, Ferrone S, Ferrone S, Gorini S, Herberman RB & Reisfeld RA (1979). Expression of HLA antigens on cultured human melanoma cells: Lack of association with melanoma associated antigens. Current Trends in Tumor Immunology 347–366 Tursi, A., Errico, G., Quaranta, V. & Specchia, G. (1977). [Experimental study of the anti-inflammatory action of pyrasanone in vitro]. Clin Ter 83, 319–324

Pending Applications U54CA209917 (Quaranta) 07/01/16 – 06/30/21 Systems Analysis of Tumor Heterogeneity This proposal is the new issue of the Integrative Cancer Biology Program for a Consortium of Cancer Systems Biology Centers. It replaces the previous NCI U54 consortium (see below, under NC extension), which was sunset after the prescribed 10 years of duration. One year elapsed for the announcement of the new program. Role: Principal Investigator R35CA197576 (Quaranta) 07/01/16 – 06/30/23 Studies on Tumor Heterogeneity This is in response to the NCI call for Outstanding Investigator Award. It is a resubmission of an A0 application that was scored 30. Role: Principal Investigator U01CA199319 (Lopez) 04/01/16 – 03/31/21 Stochastic models of signaling networks and cell fate decisions in oncogene addiction This is a collaborative proposal with a computational biologist, to predict parameters of signaling

Vito Quaranta, MD

network models, by Bayesian inference and particle swarm optimization. Model predictions will be validated with experimental data from lung cancer cells treated with kinase inhibitors. Role: co-PI Ongoing Research Support NCI R21 CA178589 (Quaranta) 09/10/14 – 08/31/16 Inhibition of proliferation by Laminin This proposal will identify novel avenues for blocking Warburg metabolism, which supports fast proliferation, based on our novel observations that cancer cell adhesion to the ECM macromolecule, Ln-332, naturally inhibits aerobic glycolysis. Role: Principal Investigator NCI U54 CA113007 (Quaranta) 05/01/10-02/28/15 (NC extension) Multiscale mathematical modeling of cancer progression The major goal of this project is to encapsulate critical biological parameters of cancer progression and drug response into mathematical models that produce novel insights into the dynamics of cancer, to be validated experimentally in iteration with model refinement. Role: Principal Investigator NCI U01 CA174706 (Yankeelov, Quaranta, Rericha) 06/01/13-05/31/18 Image driven multi-scale modeling to predict treatment response in breast cancer The major goal of this project is to develop tumor forecasting methods that predict response of individual breast cancer patients to neoadjuvant therapy by integrating in vivo advanced quantitative multi-modality imaging data into multiscale biophysical models that predict longterm outcomes of HER2+ breast cancer after a single cycle of targeted neoadjuvant therapy. Role: Co-Principal Investigator NCI R01 CA186193 (Quaranta, Yankeelov, Rericha) 04/01/14-03/31/19 Quantitative multiscale imaging to optimize cancer treatment strategies The overall goal of this project is to integrate quantitative in vitro and in vivo imaging measurements to predict the maximum patient tumor response early in the course of oncogene-targeted therapy, in order to enable alternative treatment options that minimize or prevent the emergence of the resistant phenotype. Role: Contact Principal Investigator Recently Completed Research Support Uniting Against Lung Cancer (UALC) (Tyson) 03/01/13-02/28/15 Variability of erlotinib response in lung cancer The major goal of this project is to examine how variability of erlotinib response occurring at the cellular level dictates progression of EGFR mutant-expressing lung cancer. Role: Co-Investigator NCI R01 CA047858 (Quaranta) Integrins in Cancer Invasion Role: Principal Investigator NIGMS R01 GM067221 (Quaranta)

03/01/05-05/31/13

08/01/05-07/31/10

Vito Quaranta, MD

Functional studies on Laminin-5 Role: Principal Investigator NIDDK R01 DK069921 (Zent) 03/01/05-01/31/10 Role of MT-MMPs in renal development Role: Co-Principal Investigator ------------------------------------------------------------------------------------------------------------------------------Personal Statement I have a background in cell biology, and for over 3 decades I have studied cancer invasion and the tumor microenvironment. I spent the last 12 years leading an NCI-funded (U54) Center for Cancer Systems Biology, retraining myself to apply computational and mathematical approaches to the understanding of cancer heterogeneity. These are both exciting and frustrating times for cancer research: An enormous amount of ever-growing quantitative information is at our disposal, yet cancer still appears as an impregnable bastion. I am entirely convinced that the right mixture of theory and experimentation is indispensable to understanding cancer for what it is: a complex adaptive system that responds to perturbations, such as drugs, evolving over multiple biological and temporal scales. My laboratory is completely interdisciplinary, with a mix of experimentalists, engineers, statisticians, and mathematicians, applying mathematical and computational approaches along with experimentation to understand cancer heterogeneity in response to treatment. The field of systems biology was a natural fit for me, because of my interest and ability to survey diverse scientific fields and integrate new concepts and approaches into my work. I strive to surround myself with hard-core experimentalists, modelers, statisticians, and engineers, in order to bring a new and quantitative approach to understanding fundamental scientific questions. Currently, we are developing novel quantitative approaches to derive global drug-response dynamics of cancer cell populations from single cell and clonal data. I have kept my laboratory continuously funded for over 30 years. Therefore, I know how to balance cutting edge innovative research with productivity, as well as how to maintain long-term momentum in high-risk, high-payoff projects. I am especially qualified to direct projects that require development of novel quantitative procedures at single cell and clonal levels, production and critical evaluation of large datasets, and construction of quantitative models for anticancer drug efficacy, followed by experimental validation.