CURRICULUM VITAE MARIA ANTONIETTA VANONI

1 CURRICULUM VITAE MARIA ANTONIETTA VANONI Professor of Biochemistry, Department of Biosciences, University of Milano, Via Celoria 26, 20133 Milano, ...
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CURRICULUM VITAE MARIA ANTONIETTA VANONI Professor of Biochemistry, Department of Biosciences, University of Milano, Via Celoria 26, 20133 Milano, Italy. Phone: +390250314901. Fax: +390250314895. E-mail: maria.vanoni @ unimi.it PROFESSIONAL HISTORY 2003 - present 2002- 2003 2000 - 2002 1992 - 2000

1983 - 2000

1981 - 1983

1990 - 1997 1989 1988 1987 1985

Professor of Biochemistry , University of Milano, Milano, Italy Associate Professor of Biochemistry Universita' degli Studi di Milano, Milano, Italy Associate Professor of Biochemistry Universita' dell' Insubria, Como, Italy Lecturer, Biological Chemistry, School of Chemistry, Universita' dell' Insubria (formerly, Como Branch, Universita' degli Studi di Milano) Como. Ricercatore (Assistant Professor), Department of General Physiology and Biochemistry, Universita’ degli Studi di Milano, Milano, Italy Post-doctoral Research Associate, Biophysics Research Division and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI (USA). Visiting Scientist, Emory University, School of Medicine, Atlanta, (Several 1-2 months stays ) Visiting Scientist, University of Michigan, Ann Arbor and University of Washigton, Seattle (on a leave of absence, 2 months) Visiting Scientist, Institut Pasteur, Paris (F, 2 months) Visiting Assistant Professor, University of Michigan, Ann Arbor, MI (USA) (on a leave of absence, 2 months) Visiting Scientist, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY (USA) (on a leave of absence, 3 months)

RESEARCH INTERESTS Elucidation of structure-function relationships of enzymes, especially – but not only - flavin- and/or ironsulfur cluster-dependent oxidoreductases by means of kinetic and spectroscopic approaches coupled to protein engineering and complemented by structural and computational approaches. Past projects. Mammalian Methylenetetrahydrofolate reductase (MTHFR) Mammalian D-amino acid oxidase (DAAO) Azospirillum brasilense glutamine synthetase (GS) Mycobacterium tuberculosis enzymes acting on tRNA: glutamyl tRNA synthetase (GluTS) and glutamyl

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tRNA reductase (GluRS). Computational approaches to dissect flavoenzyme mechanisms (in collaboration with Drs. E. Fois and G. Tabacchi (University of Insubria at Como) ) Ferredoxin NADP(H) reductases(FNR) from plant , Mycobacterium tuberculosis, Toxoplasma gondii and Plasmodium falciparum (In collaboration with Drs. G. Zanetti (now retired) and A. Aliverti, University of Milano) Human lysine demethylase 1 (LSD1) (in collaboration with drs. E. Battaglioli (University of Milano) and A. Mattevi (University of Pavia) Current projects Glutamate synthase (GltS). Dimethylglycine dehydrogenase. Human MICAL-1 the flavoenzyme participating in the control of actin cytoskeleton dynamics . Human Seladin1/DHCR24, the putative 24-dehydrocholestorol reductase. AIF, the apoptosis inducing factor (in collaboration with Prof. Aliverti) HIV1 protease (in collaboration with Drs. Broglia and Tiana, Department of Physics, UNIMI) Recent and current research support "Structure-function of glutamate synthase", Ministero dell' Universita' e la Ricerca Scientifica Tecnologica (MURST-60%), 1998-2000 "The three-dimensional structure as a guide for the optimisation of flavin-dependent oxidoreductases of practical interest", Consiglio Nazionale delle Ricerche, Target Project on Biotechnology .1997 - 2000 "Structural Biology - Structure and Function of redox enzymes", Ministero dell' Universita' e della Ricerca Scientifica e Tecnologica (MURST), Programmi di Ricerca di Interesse nazionale 1997 (PRIN97), 1997 – 1999. "Structure and function of redox enzymes: Mechanisms of electron transfer in flavin- and iron sulfur clusters-containing enzymes", MURST, PRIN99 (1999 – 2001), MURST, PRIN 2001 (2001-2003), PRIN2003 (2003-2005), PRIN 2005 (2005-2007). "TB prevention cluster: novel drug targets" European Union, 5th Framework Program, 2000 - 2004 “Structure-function studies of flavoenzymes”, University of Milano since 2000, "Functional, structural and genetic characterisation of Mycobacterium tuberculosis glutamyl-tRNA reductase, a key enzyme of tetrapyrrole cofactors biosynthesis".MURST, PRIN2000, 2001-2002 "Functional, structural and genetic characterisation of Mycobacterium tuberculosis glutamyl-tRNA reductase, a key enzyme of tetrapyrrole cofactors biosynthesis, CARIPLO 2005-2008, “Low resolution Structure determination of glutamate synthases by small angle X-ray scattering”, Fondazione Vigoni-DAAD, to fund exchange of personnel between the MAV lab and that of Dr. Svergun, [email protected], Hamburg (2006) Cariplo NOBEL 2005“GUARD, Genoproteomics of Age−Related Disorders” (PI at DSBB-UniMI: Prof.Martino Bolognesi, 2006-2009,). PRIN 2008: Flavin and Flavoproteins synthesis and biogenesis (PI of laboratory and Coordinator of National Network, 2010-2012) Telethon 2010: “Structure‐function studies on 24‐dehydrocholesterol reductase, the affected enzyme in desmosterolosis, a severe inherited disorder of sterol metabolism” (PI, 12/2010-6/2015) Research contracts C5,6 Italy, Research Contract: Biochemical characterization of secretomes for the conversion of lignocellosic material in simple sugars. (PI, 12/2010 - 11/2011). Axxam, Italy: Development and optimization of enzyme assays (PI, 2012- 2013,)

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Pending: MIUR-DAAD Joint Mobility Program on Structure, function and regulation of human MICAL1 (with D. Svergun, [email protected], Hamburg) MIUR PRIN 2015 Mechanism and regulation of human MICAL1, the novel multidomain flavoenzyme participating in cytoskeleton dynamics (PI) PROFESSIONAL SOCIETIES Societa' Italiana di Biochimica (1981 - ) Sigma XI- Scientific Research Society, USA (1982-1998) Biochemical Society, UK (1990 - ) Society of Biological Inorganic Chemistry (1995 - ) American Society of Biochemistry and Molecular Biology (2001 -) PROFESSIONAL ACTIVITIES Grant reviewing  Fund for Scientific Research - Flanders, Belgium, External Reviewer (1999)  European Commission, Brussels, Belgium (2001-)  Ministero della Ricerca Scientifica e tecnologica, Rome, Italy (2005- )  Wellcome Trust (UK, 2009) Journal reviewing  Archives Biochemistry and Biophysics (Member of Editorial Advisory Board since 2002)  Biochimica Biophysica Acta  Biochemistry  European Journal of Biochemistry, now FEBS Journal (Member of Editorial Advisory Board 2000 2008)  FEMS Microbiology Letters  FEBS Letters  Protein Sciences  Journal of Biological Chemistry  Journal of Bacteriology Honors - Premio Marotta - Accademia dei XL, Rome, 2007. Committees Scientific Advisory Board for the EMBL-Hamburg SAXS beamline(s) at DESY-PETRA III synchrotron (since 2006). Priority evaluation committee, EMBL-Hamburg Outstation (since 2008) INVITED LECTURES - 10th International Symposium on Flavins and Flavoproteins, Como, Italy, 1990. - E.I. DuPont de Nemours & Co., Wilmington, DE, 1991 - 37th Congresso Nazionale della Societa' Italiana di Biochimica, Perugia, Italy, 1992. - 11th International Symposium on Flavins and Flavoproteins, Nagoya, Japan, 1993. - 7th International Congress on BioInorganic Chemistry, Luebeck, Germany,1995 - Annual meeting of the Human Capital Mobility Network on Flavins and Flavoproteins (FLAPS), Wageningen, NL, 1996. - 12th International Symposium on Flavins and Flavoproteins, Calgary, Canada, 1996.

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- Department of Biochemistry, Universite' J. Fourier, Grenoble (France), 1996 - 4th European Congress on Bioinorganic Chemistry, Seville, Spain, 1998. - Department of Molecular Biology, University of Rome, Rome (Italy), 1998 - 14th Meeting of the Workgroup on Structure and Function of Proteins of the Italian Biochemical Society, Rome (Italy), 1999. - Department of Agricultural Sciences, Catholic University of Leuven, Leuven (Belgium), 2000. - 18th International Congress of Biochemistry and Molecular Biology, Birmingham, UK, 2000. - Department of Biochemistry, University College, Dublin (Ireland), 2000. - Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH, 2001. - CNRS, Marseilles, France, 2001 - University of Braunschweig, Braunschweig, Germany, 2002 - Universita' di Bari, Bari, Italy, 2002 - 14th International Symposium on Flavins and Flavoproteins, Cambridge, UK, 2002. - LEBS-CNRS Gif sur Yvette (France), 2003 - Drexel University, Philadelphia (PA, USA), 2005 - Temple University, Philadelphia (PA, USA), 2005 - Meeting of the Italian Society of Biochemistry, Riccione, September 2007. - Annual meeting of the Division of Biological Systems of the Italian Chemical Society, Montagnana, November 2007 - University of Michigan, Ann Arbor, MI, May 2007 - 16th International Symposium on Flavins and Flavoproteins, Jaca, Spain, 2008. - University of Montpellier, February 2009 - University of Pavia, April 2009. - 3rd International Symposium on Diffraction Structural Biology, Paris-Orsay and Synchrotron Soleil, 25-28 May 2010 - University of Naples, March 2011 - University of Bari, June 2011 - International Conference on Cofactors, Turku, Finland July 10-15, 2011 - Chinese Academy of Sciences, Beijin, May 2011 - University of Sao Paolo, Brazil, January 2014 Other Activities of professional significance  Local Organizing Committee, Meeting "Proteine 86" on "Nuove prospettive di ricerca nella biochimica delle proteine", Societa' Italiana di Biochimica, Gargnano (Italy) April, 10-12, 1986.  Local Organizing Committee, 10th International Symposium on Flavins and Flavoproteins, 1990  Ph.D. Thesis Committee, Mr. F. Fieschi, Universite’ Joseph Fourier, Grenoble, France, 1996  Ph.D. Thesis Committee, Ms. O. Steenhoudt, Catholic University of Leuven, Belgium, 2000  Ph.D Thesis Committee , Ms. C. Logan, University College, Dublin, Ireland, 2000  Ph.D. Thesis Committee, Ph.D. Program in Biochemistry, University of Turin, Italy, 2001  Co-organizer and Lecturer, Scuola Nazionale delle Proteine (Advanced Short Course under the sponsorship of the Italian Society of Biochemistry), L' Aquila, Italy, May 2001.  Ph.D. Thesis Admission Committee, Ph.D. Program in Environmental Sciences, University of Insubria, Como, 2001  Component of the Scientific Organizing Committee of Proteine 2002, Meeting of the Protein Group of the Italian Biochemical Society, L' Aquila, June 2002  Ph.D. Thesis Committee, Ph.D. Program in Biochemistry, University of Turin, Italy, 2003  Co-organiser “2004 ISQBP President’s Meeting” Meeting of the International Society of Quantum Biology and Pharmacology, Como, Italy, June 5-8 2004  Ph.D. Thesis Committee, Ph.D. Program in Biochemistry, University of Turin, Italy, 2004  Italian Biochemical Society, Riccione, September 2005, Chair and Organiser of Workshop on “Redox

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proteins and their cofactors” Co-organiser “Trends in Enzymology” Conference, Como, Italy, June 7-10 2006. Gordon Conference on Enzymes Coenzymes and metabolic Pathways 2006, Maine, Session Chair. Component of the Scientific Advisory Committee of the 2008 International Symposium on Flavins and Flavoproteins to be held in Spain, May-June 2008 “Trends in Enzymology” Conference, Saint Malo (F), July 2008, Discussion Leader. Ph.D. Thesis Committee, Ph.D. Program in Biochemistry, University of Pavia, Italy, 2007 Archives Biochemistry and Biophysics, Guest Editor of the Special Issue on “Enzymology in Europe” (2008). Italian Biochemical Society, Catania, September 2009, Chair and Organiser of Workshop of the Protein Group. Annual Meeting of the Italian Biochemical Society, Milano, September 2010, Member of the Organizing Committee, Chair and Organiser of Workshop on Neuronal development and neurotrasmission. Selection Committee Ricercatore (Assistant Professor) in Biochemistry, Component University of Padua (Italy), 2010 Evaluation Committee for the promotion to the rank of Associate Professor in Biochemistry, University of Arcavacata di Rende (Italy), 2010 17th International Symposium on flavins and flavoproteins, Berkley (CA), 24-29 July 2011: member of the International Advisory Committee and Discussion Leader. “Trends in Enzymology” Conference, Saint Malo (F), July 2008, Discussion Leader. Elected Co-Chair of the 4th International conference on coenzymes and cofactors, due in 2013; 2010; resigned on March 22, 2013 Member of the promotion committee of Prof. Teresa Fitzpatrick, University of Geneva (November 2014 – March 2015)

TEACHING EXPERIENCE, GRADUATE AND POST-DOCTORAL TRAINING Biological Sciences Undergraduate Courses (University of Milano) Methods in Biochemistry Lecturer (4-6 hours per year), 1984-1990 Laboratory of Experimental Biology Lecturer (2-3 series of 30 h each per year) 1990-1999 Biochemistry Lecturer ( 3-4 h per year) 1984-2000 Protein Engineering and Biocrystallography Lecturer (30 h), since 2001-2004 Biochemistry Lecturer (96 h), since 2004 Chemistry School Undergraduate Courses (University of Insubria) Biochemistry Lecturer ( 60 hours), 1992-2002 Chemistry School Undergraduate Courses (University of Milano)) Biochemistry Lecturer ( 48 hours), since 2010 School of Environmental Sciences Undergraduate Courses (University of Insubria) Biochemistry and Biochemical Methods Lecturer ( 60 hours), 2000-2002 General Biology Lecturer ( 6 hours), 2000 School of Industrial and Environmental Biotechnology, University of Milano Undergraduate Courses Genetic and Protein Engineering 2002-2004 (24 hours) Industrial Biochemistry 2002-2009 (40 hours) Biotechnological applications of Biochemistry 2010-2012 (48 hours) Mechanistic Enzymology since 2010 (24 hours)

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Students Research Fellows

PhD Students

G. Torri Tarelli L. Raddrizzani F. Fischer P. Morandi, Ph.D. O. Serebrennikova, Ph.D. D. Monti, PhD M. Riva P. Agnelli G. Fumagalli, PhD G. Caprini, PhD S. Mulazzi I. Madaschi D. Zucchini, PhD

R. Pelanda E. Verzotti P. Morandi C. Zambonelli S. Ravasio G. Fumagalli L. Dossena S. Paravisi D. Zucchini T. Vitali

Undergraduates F. Moscatelli, L. Nuzzi, M. Visentin, G. Carrea, P. Accornero, A. Cosma, M. Rescigno, D. Mazzeo, S. Zanotti, P. Fumagalli, A. Mazzoni, M. Coppola, S. Ferretti, F. Fischer, S. Ravasio, H. Stabile, B. Valzasina, C. Colombo, R. Bossi, G. Corna, A. Piana, L. Dossena, M. Riva, K. Ferrari, P. Colombi, A. Bonaldi, S. Paravisi, P. Agnelli, S. Mulazzi, R. Morosi, A. Freddi, F. Ciriello (2007), D. Zucchini (2007), L. Criaco (2008), Ileana Madaschi (2010), Teresa Vitali (2011) Mariangela Camozzi (2012), Elena Maffioletti (2013), Luca Piazza (2014), Alessandro Esposito (2015-) Several students performing 1-2 month laboratory work in fullfilment of the practical work requested by the 1st level curricula in Biological sciences and Industrial and Applied Biotechnologies.

University Committees: Organization and coordination of “Orientation activities” for perspective and current students for the School of Industrial and environmental biotechnology, since 2004 Component of the Committee for Orientation activities for perspective and current students of the Faculty of Sciences, 2004-2010. Component of the Admission Committee of the Laurea Magistrale in Biotechnology (2004-2014) Component of the committee for the reorganization of the curriculum in Industrial and Environmental Biotechnologies (2006-2008). Coordinator of the Bachelor Degree in Industrial and Environmental Biotechnology and of the Master Degree in Biomolecular Biotechnology and Bioinformatics of the University of Milano (June 2012September 2014) Member of the Steering Committee of the Department of Biosciences (May 2012-September 2014) Member of the committee for the reorganization of the bachelor degrees in Biotechnology at the University of Milano (2012-2014). Member of “Presidio della Qualita’ della Didattica di Ateneo”:in charge of setting up and implementing a quality assurance protocols in BS and master programs offered by the University of Milano ( 2012 – present). Advanced Schools.  School of Biotechnological Applications, University of Milano, Lecturer  Scuola Nazionale di Scienza delle Proteine on "Metodologie di separazione ed Analisi (Cromatografia, Elettroforesi, Ultracentrifugazione Analitica)" organized by the Societa' Italiana di Biochimica, Gruppo Struttura e Funzione delle Proteine, Urbino, Italy, 1992.  Scuola Nazionale di Scienza delle Proteine on "Metodologie di separazione ed Analisi (Cromatografia, Elettroforesi, Ultracentrifugazione Analitica)" of the Societa' Italiana di Biochimica, Gruppo Struttura e Funzione delle Proteine, Urbino, 1993, Lecturer.  Scuola Nazionale delle Proteine (Advanced School on Protein Sciences sponsored by the Italian Society of Biochemistry), L' Aquila (Italy), 2000, Lecturer.  Scuola Nazionale delle Proteine (Advanced School on Protein Sciences sponsored by the Italian Society of Biochemistry), L' Aquila (Italy), 2001, Co-organizer and Lecturer.

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EMBO Course on “Solution scattering from biological macromolecules”, EMBL Outstation at DESY, Hamburg, October 29 – November 5, 2004, Lecturer. EMBO Course on “Solution scattering from biological macromolecules”, EMBL Outstation at DESY, Hamburg, October 2006, Lecturer. PhD Program in Biochemistry (University of Milano) , Lecturer, since 2004 PhD Program in Biochemistry (University of Milano) Member of the PhD program (since 2004). Organization Workshop on Enzyme Kinetics (2008) for the PhD program in Biochemistry (University of Milano) Co-Organization Workshop on Cellular stress (with PhD program in Cell and Molecular biology) (2009) Departmental Seminar Series in Biochemistry, Organiser, since 1998 EMBO Course on “Solution scattering from biological macromolecules”, EMBL Outstation at DESY, Hamburg, October 2008, Lecturer and Discussion Leader. Ph.D. Program in Biochemistry, University of Pavia, Italy, 2009, Lecturer. PhD Program in Biochemistry (Milano): Final examination committee , member, December 2009 PhD Program in Biochemistry, University of Pavia, Final examination committee , member, February 2011. EMBO Global Lecture on Protein Structure and Function, Chinese Academy of Sciences - EMBO, Beijing (China), April 28 - May 5, 2011, Lecturer EMBO Global Lecture on Protein Structure and Function, University of Sao Paolo, Sao Paolo, Brazil, January 18 – 26 2014, Lecturer EMBO Practical Course on solution scattering , EMBL Outstation at DESY, Hamburg, October 2016, Lecturer.

PUBLICATIONS Full papers 1. M. A. Vanoni, R. Bianchi and M. P. Simonetta (1981) Kinetic parameters of D-amino acid oxidase: comparison of two experimantal designs. Istituto Lombardo (Rend. Sc.) B 115 31-35 2. M. A. Vanoni, M. P. Simonetta, and B. Curti (1981) D-amino acid oxidase in Rhodotorula gracilis. It. J. Biochem. 30 426-427 3. M. P. Simonetta, M. A. Vanoni and B. Curti (1982) D-amino acid oxidase activity in the yeast Rhodotorula gracilis . FEMS Microbiol. Lett. 15, 27-31 4. M. A. Vanoni, D. P. Ballou, and R. G. Matthews (1983) Methylenetetrahydrofolate reductase: steadystate and rapid reaction studies on the NADPH-methylenetetrahydrofolate, NADPH-menadione and methyltetrahydrofolate-menadione oxidoreductase activities of the enzyme. J.Biol. Chem. 258, 1151011514. 5. R. G. Matthews , M. A. Vanoni, J. F. Hainfeld and J. Wall (1984) Methylenetetrahydrofolate reductase: evidence for spacially distinct subunit domains obtained by scanning transmission electron microscopy and limited proteolysis. J. Biol. Chem. 259, 11647-11650. 6. M. A. Vanoni and R. G. Matthews (1984) Kinetic isotope effect studies on the oxidation of reduced nicotinamide adenine dinucleotide phosphate by the flavoprotein methylenetetrahydrofolate reductase. Biochemistry, 23, 5272-5279. 7. M. P. Simonetta, M. A. Vanoni, P. Casalin, and M. Rusnati (1985) D-amino acid oxidase from Rhodotorula gracilis: purification and preliminary characterization. It. J. Biochem. 34 52A-54A. 8. M. P. Simonetta, M. A. Vanoni and P. Casalin (1987) Purification and properties of D-amino acid oxidase, an inducible flavoenzyme from Rhodotorula gracilis. Biochim. Biophys. Acta 914 136-142. 9. M.A. Vanoni, M. P. Simonetta, B. Curti, A. Negri, and S. Ronchi (1987) Phenylglyoxal modification of arginines in mammalian D-amino acid oxidase. Eur. J. Biochem. 167, 261-267. 10. K. K., Wong, M. A. Vanoni, and J. S. Blanchard (1988) Glutathione reductase: solvent equilibrium and kinetic isotope effects. Biochemistry, 27, 7091-7096. 11. M. A. Vanoni, S. Lee ,H. G. Floss, and R. G. Matthews (1990) Stereochemistry of reduction of

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methylenetetrahydrofolate to methyltetrahydrofolate catalyzed by pig liver methylenetetrahydrofolate reductase. J. Am. Chem. Soc. 112 , 3987-3992. 12. M. A. Vanoni, A. Negri, G. Zanetti, S. Ronchi and B. Curti (1990) Structural studies on the isolated subunits of glutamate synthase from Azospirillum brasilense Biochim. Biophys. Acta, 1039, 374-377. 13. M. A. Vanoni, K. K. Wong, D. P. Ballou, and J. S. Blanchard (1990) Glutathione reductase: comparison of steady-state and rapid reaction primary kinetic isotope effects exhibited by the yeast, spinach and Escherichia coli enzymes. Biochemistry 29 , 5790-5796. 14. G. Torri Tarelli, M. A. Vanoni, A. Negri, and B. Curti (1990) Limited proteolysis studies on mammalian D-amino acid oxidase J. Biol. Chem., 265, 21242 - 21246. 15. Frascotti, G., Coccetti, P., Vanoni, M. A., Alberghina, L., Martegani, E. (1991) The overexpression of the 3' terminal region of the CDC25 gene of Saccharomyces cerevisiae causes growth inhibition and alteration of purine nucleotides pools. Biochim. Biophys. Acta, 1089, 206-212. 16. M. A. Vanoni, L. Nuzzi, M. Rescigno, G. Zanetti, B. Curti (1991) On the kinetic mechanism of the reactions catalyzed by the glutamate synthase from Azospirillum brasilense Eur. J. Biochem. 202, 181189 17. M. A. Vanoni, D. E. Edmondson, M. Rescigno, G. Zanetti, B. Curti (1991) Mechanistic studies on Azospirillum brasilense glutamate synthase , Biochemistry, 30, 11478-11484 18. M. A. Vanoni, D.E. Edmondson, G. Zanetti, B. Curti (1992) Characterization of the flavin cofactors and the iron-sulfur centers of glutamate synthase from Azospirillum brasilense by absorption, circular dichroism and electron paramagnetic resonance spectroscopies. Biochemistry, 31, 4613-4623. 19. R. Pelanda, M. A. Vanoni, M. Perego, L. Piubelli, A. Galizzi, B. Curti, and G. Zanetti (1993) Glutamate synthase genes of the diazotroph Azospirillum brasilense. Cloning, sequencing and analysis of functional domains. J. Biol. Chem. 268 , 3099-3106 20. M. A. Vanoni, P. Accornero, G. Carrera, and B. Curti (1994) The pH dependent behaviour of catalytic activities of Azospirillum brasilense glutamate synthase and iodoacetamide modification of the enzyme provide evidence for a catalytic Cys-His ion pair. Arch. Biochem. Biophys. 309, 222-230. 21. M. A. Vanoni, A. Mazzoni, P. Fumagalli, A. Negri, G. Zanetti, B. Curti (1994) Probing the domain structure of Azospirillum brasilense glutamate synthase, a complex iron-sulfur flavoprotein: limited proteolysis studies and characterization of the diaphorase activities of the enzyme. Eur. J. Biochem. 226, 505-515. 22. A. Aliverti, C. M. Bruns, V. E. Pandini, P.A. Karplus, M.A. Vanoni, B. Curti, and G. Zanetti (1995) Involvement of serine96 in the catalytic mechanism of ferredoxin-NADP+ reductase: Structurefunction relationship as studied by site-directed mutagenesis and X-ray crystallography. Biochemistry, 34, 8371-8379. 23. M.A. Vanoni, E. Verzotti, G. Zanetti, B. Curti (1996) Glutamate synthase: properties of the recombinant  subunit. Eur. J. Biochem. 236, 937-946. 24. A. Mattevi, M.A. Vanoni, F. Todone, M. Rizzi, A. Teplyakov, A. Coda, M. Bolognesi, B. Curti (1996) Crystal structure of D-amino acid oxidase: a case of mirror-image convergent evolution with flavocytochrome b2. Proc. Natl. Acad of Sciences, USA, 93, 7496-7501. 25. M.A. Vanoni, A. Cosma, D. Mazzeo, A. Mattevi, F. Todone, B. Curti (1997) Limited proteolysis and X-ray crystallography reveal the origin of substrate specificity and of the rate limiting product release during oxidation of D-amino acids catalysed by mammalian D-amino acid oxidase. Biochemistry 36, 5624-5632. 26. F. Todone, M.A. Vanoni, A. Mozzarelli, M. Bolognesi, A. Coda, B. Curti, A. Mattevi (1997) Active site plasticity in D-amino acid oxidase: a crystallographic analysis. Biochemistry 36 5853-5860. 27. A. Mattevi, M.A. Vanoni, B. Curti (1997) Structure of D-amino acid oxidase: new insights from an old enzyme. Curr. Op. Struc. Biol. 7, 804-810. 28. M.A. Vanoni, B. Curti (1997) Modular architecture of glutamate synthase, a complex iron-sulfur flavoprotein. Biochimica italiana, 7, 17-28. 29. M.A. Vanoni, F. Fischer, S. Ravasio, E. Verzotti, D.E. Edmondson, W.R. Hagen, G. Zanetti, B. Curti (1998) The recombinant  subunit of glutamate synthase: spectroscopic and catalytic properties. Biochemistry 37, 1828-1838. 30. V. Niviere, M.A. Vanoni, G. Zanetti, M. Fontecave (1998) The Escherichia coli flavin reductase:

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characterization of reaction intermediates. Biochemistry 37, 11879-11887. 31. K. Rosenbaum, K. Jahnke, B. Curti, W.R. Hagen, K. Schnackerz, M.A. Vanoni. (1998) Porcine recombinant dihydropyrimidine dehydrogenase: comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes. Biochemistry 37, 17598-17609. 32. M.A. Vanoni and B. Curti (1999) Glutamate synthase: a complex iron-sulfur flavoprotein. Cell. Mol. Life Sci. 55, 617-638. 33. Morandi, P., Valzasina, B., Colombo, C., Curti, B., Vanoni, M.A. (2000) Glutamate synthase:Identification of the NADPH binding site by site-directed mutagenesis. Biochemistry, 39, 727-735. 34. Stabile, H., Curti, B., Vanoni, M.A. (2000) Functional Properties of recombinant Azospirillum brasilense glutamate synthase, a complex iron-sulfur flavoprotein. Eur. J. Biochem. 267, 2720-2730. 35. Hagen, W.R., Vanoni, M.A., Rosenbaum, K., Schnackerz, K.S. (2000) On the iron-sulfur clusters in the complex redox enzyme dihydropyrimidine dehydrogenase. Eur. J. Biochem. 267, 3640-3646. 36. C. Binda, R. Bossi, S. Wakatsuki, S. Artz, A. Coda, B. Curti, M.A. Vanoni, A. Mattevi (2000) Crosstalk and ammonia channeling between active centres in the unexpected domain arrangement of glutamate synthase. Structure 8, 1299-1308. 37. L. P. Antonyuk, V. E. Smirnova, A.A. Kamnev ,O. B.Serebrennikova , M. A. Vanoni, G. Zanetti , I. A. Kudelina , O. I. Sokolov, V. V. Ignatov (2001) Influence of divalent cations on the catalytic properties and secondary structure of unadenylylated glutamine synthetase from Azospirillum brasilense BioMetals 14, 13–22. 38. S. Ravasio, B. Curti, M.A. Vanoni (2001) Determination of the mid-point potential of the FAD and FMN flavin cofactors and of the 3Fe/4S cluster of glutamate synthase. Biochemistry, 40, 5533-5541. 39. M. Terao, M. Kurosaki, M.Marini, M.A.Vanoni, G. Saltini, V. Bonetto, A. Bastone, C.Federico, S. Saccone, R. Fanelli, M. Salmona, and E. Garattini. (2001) Purification of AOH1 and cloning of the AOH1 and AOH2 genes. Identification of a novel molybdo-flavoprotein gene cluster on mouse chromosome 1. J. Biol. Chem., 276, 46347-46363. 40. S. Ravasio, L. Dossena, E. Martin-Figueroa, F.J. Florencio, A. Mattevi, P. Morandi, B. Curti and M.A. Vanoni (2002) Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated  subunit. Biochemistry , 41, 8120-8133 41. R. H.H. van den Heuvel, D. Ferrari, R.T. Bossi, S. Ravasio, B. Curti, M.A. Vanoni, F.J. Florencio and A. Mattevi (2002) Structural studies on the synchronization of catalytic centers in glutamate synthase. J. Biol. Chem. , 277, 24579-24583. 42. Tilocca, A., Gamba, A., Vanoni, M.A., Fois, E. (2002) First-principles molecular dynamics investigation of the D-amino acid oxidative half reaction catalyzed by the flavoenzyme D-amino acid oxidase. Biochemistry,41, 14111-14121. 43. van den Heuvel, R.H.H., Svergun, D.I., Petoukov, M.V., Coda, A., Curti, B., Ravasio, S., Vanoni, M.A. and Mattevi, A. (2003) The active conformation of glutamate synthase and its binding to ferredoxin. J. Mol. Biol. 330, 113-128. 44. Petoukov, M.V., Svergun, D.I., Konarev, P.V., Ravasio, S., van den Heuvel, R.H.H., Curti, B., and Vanoni, M.A. (2003) Quaternary structure of Azospirillum brasilense NADPH-dependent glutamate synthase in solution as revealed by synchrotron radiation X-ray scattering. J. Biol. Chem. 278, 2993329939. 45. Zambonelli, C., Morandi, P., Vanoni, M.A., Tedeschi, G., Servi, S., Curti, B., Carrea, G., Di Lorenzo, R. and Monti, D. (2003) Cloning and expression in E. coli of the gene encoding Streptomyces PMF PLD, a phospholipase D with high transphosphatidylation activity. Enzyme and Microbial Technology 33, 676-688. 46. Conti P, Roda G, Stabile H, Vanoni MA, Curti B, De Amici M. (2003) Synthesis and biological evaluation of new amino acids structurally related to the antitumor agent acivicin. Farmaco 58, 683690. 47. van den Heuvel, R.H.H., Curti, B, Vanoni, M.A. and Mattevi, A. (2004) Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate Cell. Mol. Life Sci. 61, 669-681. 48. Coiro VM, Di Nola A, Vanoni MA, Aschi M, Coda A, Curti B, Roccatano D. (2004) Molecular

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dynamics simulation of the interaction between the complex iron-sulfur flavoprotein glutamate synthase and its substrates. Protein Sci. 13 2979-2991. 49. Vanoni, M.A., Dossena, L., van den Heuvel, R.H.H. and Curti, B. (2005) Structure-function studies on the complex iron-sulfur flavoprotein glutamate synthase: the key enzyme of ammonia assimilation. Photosynthesis research 83, 219-238 50. Vanoni, M.A. and Curti, B. (2005) Structure-function studies on the iron-sulfur flavoenzyme glutamate synthase: an unexpectedly complex self-regulated enzyme. Arch. Biochem. Biophys. 433, 193-211 51. Agnelli, P., Dossena, L., Colombi, P., Mulazzi, S., Morandi, P., Tedeschi, G., Negri, A., Curti, B., and Vanoni, M.A. (2005) The unexpected structural role of glutamate synthase [4Fe-4S]+1, +2 clusters as demonstrated by site-directed mutagenesis of conserved C residues at the N-terminus of the enzyme  subunit. Arch. Biochem. Biophys. 436, 355-366. 52. Forneris, F., Binda, C., Vanoni, M.A., Mattevi, A. and Battaglioli, E. (2005) Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process. FEBS Lett., 579, 2203-2207. 53. Forneris, F., Binda, C., Vanoni, M.A., Battaglioli, E.and Mattevi, A. (2005) Human histone demethylase LSD1 reads the histone code. J Biol Chem. 280 41360-41365 54. Forneris, F., Binda, C., Vanoni, M.A., Mattevi, A., and Battaglioli, E. (2006) Demethylation pathways for histone methyllysine residues. In The Enzymes (Tamanoi, F and Clarke, S. Eds), Vol. 24, Chapter 8, Academic Press, NY . 55. Vanoni, M.A. and Curti, B. (2006) D-amino acid oxidase activity assays. In “D-Amino Acids: A New Frontier in Amino Acid and Protein Research -Practical Methods and Protocols” (R. Konno, H. Brueckner, A. D'Aniello, G. Fisher, N. Fujii, and H. Homma, Eds) Nova Science Publishers, Inc., Hauppage (NY) , pp 467-476. 56. Andrea Pennati, Adelia Razeto, Matteo de Rosa, Vittorio Pandini, Maria Antonietta Vanoni, Andrea Mattevi, Alessandro Coda, Alessandro Aliverti and Giuliana Zanetti (2006) Role of the His57-Glu214 Ionic Couple Located in the Active-Site of Mycobacterium tuberculosis FprA Biochemistry, 45, 87128720.¶ 57. Dossena, L., Curti, B., Vanoni, M.A. (2007) “Activation and coupling of the glutaminase and synthase reaction of glutamate synthase is mediated by E1013 of the ferredoxin-dependent enzyme, belonging to loop 4 of the synthase domain” Biochemistry, 46, 4473-4485. 58. Tabacchi, G., Vanoni, M. A., Gamba, A. & Fois, E. (2007) Does negative hyperconjugation assist enzymatic dehydrogenations?, Chemphyschem. 8, 1283-1288. 59. Vanoni, M. A. & Curti, B. (2008) Structure-function studies of glutamate synthases: a class of selfregulated iron-sulfur flavoenzymes essential for nitrogen assimilation, IUBMB Life. 60, 287-300 60. Vanoni, M. A. (2008) Enzymology in Europe and Trends in Enzymology 2008, Arch Biochem Biophys.474, 225. 61. Cottevieille, M., Larquet, E., Jonic, S., Petoukhov, M. V., Caprini, G., Paravisi, S., Svergun, D. I., Vanoni, M. A. & Boisset, N. (2008) The Subnanometer Resolution Structure of the Glutamate Synthase 1.2-MDa Hexamer by Cryoelectron Microscopy and Its Oligomerization Behavior in Solution: FUNCTIONAL IMPLICATIONS, J Biol Chem. 283, 8237-8249. 62. Paravisi, S., Fumagalli, G., Riva, M., Morandi, P., Morosi, R., Konarev, P. V., Petoukhov, M. V., Bernier, S., Chenevert, R., Svergun, D. I., Curti, B. & Vanoni, M. A. (2009) Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl-tRNA synthetase and determination of its oligomeric structure in solution, FEBS J. 276, 1398-1417. 63. Tabacchi, G., Zucchini, D., Caprini, G., Gamba, A., Lederer, F., Vanoni, M. A. & Fois, E. (2009) Llactate dehydrogenation in flavocytochrome b2: a first principles molecular dynamics study, FEBS J. 276, 2368-2380. 64. Crobu D, Canevari G, Milani M, Pandini V, Vanoni MA, Bolognesi M, Zanetti G, Aliverti A. (2009) Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis. Biochemistry. 48, 9525-9533. 65. Gianazza E, Eberini I, Sensi C, Barile M, Vergani L, Vanoni MA (2011) Energy matters: mitochondrial proteomics for biomedicine. Proteomics. 4,657-674. doi: 10.1002/pmic.201000412.

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66. Zucchini D, Caprini G, Pasterkamp RJ, Tedeschi G, Vanoni MA. (2011) Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1. Arch Biochem Biophys. [doi:10.1016/j.abb.2011.08.004] 67. Baroni, S.; Pandini, V.; Vanoni, M.A.; Aliverti, A. (2012) A single tyrosine hydroxyl group almost entirely controls the NADPH specificity of Plasmodium falciparum ferredoxin-NADP+ reductase. Biochemistry, 51, 3819-3826. 68. Vanoni, M.A.; Vitali, T.; Zucchini, D. (2013) MICAL, the Flavoenzyme Participating in Cytoskeleton Dynamics. Int J Mol Sci, 14, 6920-6959. 69. Caldarini M, Sonar P, Valpapuram I, Tavella D, Volonté C, Pandini V, Vanoni MA, Aliverti A, Broglia RA, Tiana G, Cecconi C. (2014) The complex folding behavior of HIV-1-protease monomer revealed by optical-tweezer single-molecule experiments and molecular dynamics simulations. Biophys Chem. 195, 32-42. doi: 10.1016/j.bpc.2014.08.001. 70. Sorrentino L, Calogero AM, Pandini V, Vanoni MA, Sevrioukova IF, Aliverti A. (2015) Key Role of the Adenylate Moiety and Integrity of the Adenylate-Binding Site for the NAD(+)/H Binding to Mitochondrial Apoptosis-Inducing Factor. Biochemistry. 54, 6996-7009. doi: 10.1021/acs.biochem.5b00898. 71. Vanoni MA. (2015) Glutamate synthase: A case-study for in silico drug screening on a complex ironsulfur flavoenzyme? Gene, 564, 233-235. doi: 10.1016/j.gene.2015.04.003 72. Vitali T, Maffioli E, Tedeschi G, Vanoni MA (2016) Properties and catalytic activities of MICAL1, the flavoenzyme involved in cytoskeleton dynamics, and modulation by its CH, LIM and C-terminal domains. Arch Biochem Biophys., 593:24-37. doi: 10.1016/j.abb.2016.01.016.

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2. 3. 4. 5. 6. 7. 8. 9.

Chapters in Books M. A. Vanoni, C. S. Daubner, D. P. Ballou and R. G. Matthews (1983) Studies on methylenetetrahydrofolate reductase from pig liver: catalytic mechanism and regulation by adenosylmethionine. In: Chemistry and Biology of Pteridines (Blair, J. A., ed.) De Gruyter, Berlin, 235239 M. P. Simonetta, S. Galliani, M. A. Vanoni, S. Ronchi and B. Curti (1984) The role of arginines in Damino acid oxidase. In: Flavins and Flavoproteins (Bray, R. C., Engel, P. C. and Mayhews S. G. eds.) De Gruyter, Berlin, 295-299. M. A. Vanoni and R. G. Matthews (1984) Methylenetetrahydrofolate reductase: an imperfect enzyme? In: Flavins and Flavoproteins (Bray, R. C., Engel, P. C. and Mayhews S. G. eds.) De Gruyter, Berlin, 497-500. R. G. Matthews, M. A. Vanoni and S. Khani (1984) Evidence for two spacially distinct domains on each subunit of methylenetetrahydrofolate reductase. In: Flavins and Flavoproteins (Bray, R. C., Engel, P. C. and Mayhews S. G. eds.) De Gruyter, Berlin, 217-220. S. Ratti, M. A. Vanoni, R. Grandori, G. Zanetti, and B. Curti (1987) Kinetic studies on glutamate synthase from Azospirillum brasilense. In: Flavins and Flavoproteins 1987 (D.E. Edmondson, D.B. McCormick eds.) De Gruyter, Berlin, 385-389. M. A. Vanoni, K. K. Wong and J. S. Blanchard (1987) Kinetic isotope effect studies on yeast, spinach and human erythrocyte glutathione reductases. In: Flavins and Flavoproteins 1987 (D.E. Edmondson, D.B. McCormick eds.) De Gruyter, Berlin, 55-58. M. P. Simonetta, P. Casalin, L. Pollegioni, M. A. Vanoni and B. Curti (1987) D-amino acid oxidase from the yeast Rhodotorula gracilis. In: Flavins and Flavoproteins 1987 (D. E. Edmondson, D. B. McCormick Eds.) De Gruyter, Berlin, 205-208. M. A. Vanoni, D. E. Edmondson, G. Zanetti, B. Curti (1991) Glutamate synthase from Azospirillum brasilense: role of flavins and iron sulfur centers. In: Flavins and Flavoproteins 1990 (Curti, B., Ronchi, S., and Zanetti, G., eds.) De Gruyter, Berlin, pp. 749 - 753. G. Torri Tarelli, M. A. Vanoni, A. Negri, B. Curti, (1991) Limited proteolysis studies on the apo-, holo-, holoenzyme-benzoate forms of pig kidney D-amino acid oxidase. In: Flavins and Flavoproteins 1990

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(Curti, B., Ronchi, S., and Zanetti, G., eds.) De Gruyter, Berlin, pp. 155- 158. 10. M. A. Vanoni, L. Nuzzi, M. Rescigno, M. Visentin, P. Accornero, R. Pelanda, M. P. Simonetta, G. Zanetti, B. Curti (1991) Kinetic and structural studies on glutamate synthase from Azospirillum brasilense. In: Flavins and Flavoproteins 1990 (Curti, B., Ronchi, S., and Zanetti, G., eds.) De Gruyter, Berlin, pp. 755-759. 11. M. A. Vanoni, S. Lee, H. G. Floss, R. G. Matthews (1991) The stereochemistry of reduction of methylenetetrahydrofolate to methyltetrahydrofolate catalyzed by pig liver methylenetetrahydrofolate reductase. In: Flavins and Flavoproteins 1990 (Curti, B., Ronchi, S., and Zanetti, G., eds.) De Gruyter, Berlin, pp. 815 - 818. 12. M. A. Vanoni, B. Curti, and G. Zanetti (1992) Glutamate synthase. In " Chemistry and Biochemistry of Flavoenzymes" (Muller, F., Ed.) Vol. 3, Chapter 9, pp. 309-317, CRC Press, Boca Raton. 13. M. A. Vanoni (1994) Glutamate synthase from Azospirillum brasilense : structural and mechanistic studies. In "Flavins and Flavoproteins 1993" (Yagi, K. , Ed.) de Gruyter, Berlin, 667-674. 14. R. Pelanda, L. Piubelli, P. Fumagalli, A. Mazzoni, E. Verzotti, M. A. Vanoni, G. Zanetti, and B. Curti (1994) Cloning and expression of Azospirillum brasilense glutamate synthase. In "Flavins and Flavoproteins 1993" (Yagi, K. , Ed.) de Gruyter, Berlin, 675-683. 15. B. Curti, M.A. Vanoni, E. Verzotti, G. Zanetti (1996) Glutamate synthase: a complex iron-sulfur flavoprotein. Biochemical Society Transactions, 24 , 95-99. 16. F. Todone, A. Mattevi, M.A. Vanoni, B. Curti (1997) Crystal structure of D-amino acid oxidase. In “Flavins and Flavoproteins 1996” ( Stevenson, K., Massey, V., and Williams, C. H., Eds), pp. 177-185 Calgary University Press, Calgary. 17. M.A. Vanoni, E. Verzotti, F. Fischer, M. Coppola, S. Ferretti, G. Zanetti, B. Curti (1997) Glutamate synthase of Azospirillum brasilense. In “Flavins and Flavoproteins 1996” (Stevenson, K., Massey, V., and Williams, C. H., Eds), pp. 879-888. 18. Aliverti, B. Curti, M.A. Vanoni (1999) Identifying and quantitating FAD and FMN in simple and ironsulfur-containing flaviproteins. In: Methods in Molecular Biology, Vol. 131, Flavoprotein Protocols (Chapman, S. K. and Reid, G. A., Eds.), pp. 9-23, Humana Press Inc., Totowa, NJ, USA. 19. Aliverti, A., Fischer, F., Pasquini, S., Vanoni, M. A., Curti, B., Zanetti, G., Cantoni, R., Branzoni, M., Riccardi, G. (1999) Characterization of an adrenodoxin reductase-like protein of Mycobacterium tuberculosis. In: Flavins and Flavoproteins 1999, (Ghisla, S., Kroneck, P., Macheroux, P. and Sund, H., Eds.) pp. 891-894, Agency for Scientific Publications, Berlin. 20. Vanoni, M.A., Ravasio, S., Stabile, H., Bossi, R., Zanetti, G., Curti, B. (1999) Azospirillum brasilense glutamate synthase overproduction in E. coli and characterisation of the recombinant enzyme. In: Flavins and Flavoproteins 1999, (Ghisla, S., Kroneck, P., Macheroux, P. and Sund, H., Eds.) pp. 799-802, Agency for Scientific Publications, Berlin. 21. Morandi, P., Valzasina, B., Colombo, C., Vanoni, M.A., Curti, B. (1999) Azospirillum brasilense glutamate synthase: identification of substrates and cofactors binding sites by site-directed mutagenesis. In: Flavins and Flavoproteins 1999, (Ghisla, S., Kroneck, P., Macheroux, P. and Sund, H., Eds.) pp. 787790, Agency for Scientific Publications, Berlin. 22. Niviere, V., Vanoni, M.A., Fieschi, F., Fontecave, M. (1999) Flavin reductase of Escherichia coli: Structure-activity studies. In: Flavins and Flavoproteins 1999, (Ghisla, S., Kroneck, P., Macheroux, P. and Sund, H., Eds.) pp. 289-292, Agency for Scientific Publications, Berlin. 23. P. Morandi, S. Ravasio, H. Stabile, B. Valzasina, F. Fischer, M.A. Vanoni (2000) Structure-function relationships of glutamate synthase. In "Nitrogen in a sustainable ecosystem. From the cell to the plant" (Martins-Loucao, M.A. & Lips, S.H., Eds) pp. 75-81. Backhuys Publishers, Leiden. 24. M.A. Vanoni, S. Ravasio, P. Morandi, L. Dossena, B. Curti (2002) Structure-function studies of glutamate synthases. In "Flavins and Flavoproteins 2002" (S. Chapman, R. Perham, N. Scrutton, Eds.) pp. 91-100, Weber Agency for Scientific Publications, Berlin . 25. R.H.H. van den Heuvel, R.T. Bossi, A. Mattevi, M.A. Vanoni (2002) Crystallographic Studies on the Inactive Conformation of Ferredoxin-dependent Glutamate Synthase. In "Flavins and Flavoproteins 2002" (S. Chapman, R. Perham, N. Scrutton, Eds.) pp. 211-216, Weber Agency for Scientific Publications, Berlin 26. B. Curti, L. Dossena, P. Agnelli, D. Svergun, V. Coiro, M.A. Vanoni (2005) Insights on glutamate synthase structure, conformational flexibility and control of catalysis from small angle X-ray scattering,

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27. 28. 29. 30. 31.

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33.

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molecular dynamics and site-directed mutagenesis studies. In “Flavins and Flavoproteins 2005” (Nishino, T., Miura, R., Tanokura, M. , Fukui, K. Eds.), 479-484 M.A. Vanoni, G. Tabacchi, F. Lederer, A. Gamba, E. Fois, (2005) Ab inizio molecular dynamics simulations of the reductive half reaction of L-lactate dehydrogenase (flavocytochrome b2). . In “Flavins and Flavoproteins 2005” (Nishino, T. , Miura, R., Tanokura, M. , Fukui, K. Eds.), 281-286 A. Aliverti, A. Pennati, V. Pandini, M.A. Vanoni, G. Zanetti, (2005) The role of the active site residues His57 and Glu214 of FprA, a Mycobacterium tuberculosis homolog of adrenodoxin reductase. . In “Flavins and Flavoproteins 2005 (Nishino, T. , Miura, R., Tanokura, M. , Fukui, K. Eds.), 491-495 E. Battaglioli, F. Forneris, C. Binda, A. dall’ Aglio, M.A. Vanoni, A. Mattevi (2005) A new flavoprotein involved in transcriptional regulation . In “Flavins and Flavoproteins 2005” (Nishino, T. , Miura, R., Tanokura, M. , Fukui, K. Eds.) pp. 751-.759 Vanoni, M.A. and Curti, B. (2007) D-amino acid oxidase activity assays. In: “D-amino acids: A new frontier in amino acid and protein research” (Konno, R., Brueckner, H., Fisher, G., Fujii, N., Homma, H., Eds) , Ch. 6.6, pp. 467476, Nova Biomedical Books, New York Vanoni M.A., Curti B., Dossena L., Caprini G., Paravisi S., Wohlever, M.L., Cottevieille M., Larquet E., Jonic S., Boisset N., Petoukhov M.V., Svergun D.I. “Glutamate synthase: self-regulation and oligomeric structure”. In Flavins and Flavoproteins 2008 (Frago, S., Gomez-Moreno, C., Medina, M., Eds), pp. 387-400, Prensas Universitarias de Zaragoza. Zucchini, D.; Madaschi, I.; Galbiati, R.; Maggi, R.; Aliverti, A.; Tedeschi, G.; Caruso, D.; Vanoni, M.A. 24-dehydrocholesterol reductase, the affected enzyme in desmosterolosis, a severe inherited disorder of cholesterol metabolism In Flavins and Flavoproteins 2011; Miller, S.; Palfey, B. A.; Hille, R., Eds.; Lulu Publisher, pp. 607-612 (2013) Zucchini, D.; Caprini, G.; Vitali, T.; Pasterkamp, R.J.; Tedeschi, G.; Vanoni, M.A. Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1. In Flavins and Flavoproteins 2011; Miller, S.; Palfey, B. A.; Hille, R., Eds.; Lulu Publisher: 2012; pp. 321-326 (2013). Vanoni, M.A. Glutamate synthase. In Flavoproteins; Hille, R.; Miller, S.; Palfey, B. A., Eds.; Walter De Gruyter: Berlin, 2012; Vol. 1, pp. 271-295 (2013).

Poster presentations: over 100 presented posters at various conferences