Cell & Gene Therapy. Posters

228th OMICS Group Conference 3rd International Conference and Exhibition on Cell & Gene Therapy October 27-29, 2014 Embassy Suites Las Vegas, USA ...
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228th OMICS Group Conference

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Posters

Cell Therapy-2014

Page 147

Salib A A et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

New strategies to activate pancreatic cell therapy, therapeutic potentials of α-lipoic acid, vanadyl acetylacetonate and Nigella sativa in diabetic rats Salib A A1, Helmy Salem A H1, Zohdy M2 and Elmosalamy S H2 University of Cairo, Egypt 2 University of Oakland, USA 1

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earching for insulin replacements or insulin-mimetic upon oral administration as diabetic patients, still develop complications even with intensive insulin treatment. Rats were rendered diabetic by injection of streptozotocin (60 mg/ kg body weight). After confirmation of diabetes, rats randomly allocated into 5 groups (15 rats/group): Control (normal), diabetic, the treated groups daily received oral doses of 100 mg/kg of Alpha lipoic acid, vanadyl acetylacetonate group were received 6 mg/kg of vanadyl acetylacetonate and Nigella sativa group were received oral doses of 500 mg/kg of Nigella sativa via stomach tube for 6 weeks. After 2, 4 and 6 weeks from onset of diabetes, serum and fluoride blood samples were obtained for evaluation of fasting blood glucose, lipid metabolism evaluation (total cholesterol, triglyceride, HDL and LDL), liver functions (ALT, AST, ALP activities and total bilirubin) and kidney functions ( urea, uric acid, total protein and albumin). After 6 weeks, histopathological samples were taken from the liver, kidney and pancreas. Administration of alpha lipoic Acid, Nigella sativa and vanadyl acetylacetonate to diabetic rats resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL , ALT, AST, ALP, total bilirubin, urea, uric acid while HDL, total protein and albumin level were markedly increased compared to untreated diabetic rats. Also histopathological findings in liver, kidney of treated group showed improvement when compared with those of diabetic group while histopathological findings of pancreas in all treated group revealed regeneration in the islands of langerhans cells while those of diabetic group showed atrophy in islands of langerhans cells, degeneration in the acini and hyalinization in the wall of congested blood vessels. The results of this study indicate that the alpha lipoic Acid, Nigella sativa and vanadyl acetylacetonate possess hypoglycemic, hypolipidemic, improvement effects on kidney and liver functions and protective effects on beta cells of pancreasin STZ-induced diabetic rats. The improvement action of alpha lipoic may attributed to reducing the oxidative stress in diabetes and its hypoglycemic effect through decreasing hepatic glucose output and increase glucose uptake through increasing glucose transporter (GLUT) 1 and 4 translocation, moreover it has insulin mimetic actions. The effect of vanadyl may be due to its improvement action on hepatic glucose metabolism as its insulin mimic action while the action of Nigella attributed to its antioxidant properties which decrease oxidative stress in diabetes and improves its complications through hypoglycemic action and hepatic insulin sensitivity and decrease gluconeogenesis. Also Nigella has insulin mimetic properties. The most improvement in diabetic state was seen in alpha lipoic group then vanadyl acetylacetonate group however Nigella sativa group significally improve kidney functions than vanadyl acetylacetonate. Biography Salib A A has completed his DVM and PhD at age of 28 from Faculty of Veterinary Medicine, Department of Physiology he promoted to full Professor at age of 39 at University of Cairo Egypt. He serves as the Head of the Department of Physiology for 6 years, Senior Research Director, University of Alberta Canada. He works as Senior Research Fellow, Department of Medicine, Haematology Division, School of Medicine, Ann Arber, MI. and Research Fellow, Department of Pathology, University of Maryland, School of Medicine, Baltimore, MD. He has published more than 70 papers in reputed national, international and is serving as an Editorial Board Member of reputed journals and also has awards from NIH, USA and From QAAP, Egypt. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 148

Daniel Diaz et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Synergetic effect of PI3K inhibition and Bcl2/STAT5 knockdown in breast cancer cell lines Daniel Diaz, Alžběta Filipová, Hana Hrebíková, Rishikaysh Pisal, Kapil Chauhan, Wasay Mohiuddin, Jaroslav Mokry and Stanislav Filip Charles University, Czech Republic

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I3K signaling cascade is often hyperactivated in human cancer and is involved in both vertical and horizontal resistance to different targeted therapies. Inhibition of PI3K often show limited efficacy due to the reactivation of PI3K/AKT signaling. Kinases of the Janus family (JAKs) and their effector molecules (STATs) also play an important role in cancer since they are capable of stimulating cell proliferation, differentiation, migration, and survival. This pathway is activated upon binding of hormones and cytokines which are secreted by cancer cells and by cells from the tumor microenvironment. JAK2 is a known activator of PI3K and it is possible that JAK2 activation induced by PI3K inhibition contributes to AKT reactivation. Analyses of cytokine profiles after PI3K inhibition resulted in expression changes of several cytokines, IL-8 upregulation. Secreted IL-8 activates JAK2/STAT5 in breast cancer cells with a higher expression of CXCR receptors. It has been previously reported that activation of BIM and downregulation MCL1 are required for induction of cell death. The PI3K and JAK2 pathways activate the pro-survival protein MCL1 and suppress the pro-apoptotic protein BIM. Combined inhibition of PI3K/mTOR and JAK2 activates BIM and concomitantly downregulates MCL1, causing an increase in apoptosis, reduced tumor seeding and metastasis. Bcl-2 may serve as a target in the treatment of breast cancer as it is a known anti-apoptotic protein. Previous reports suggest that NF-kB and PI3K may contribute to Bcl-2 up-regulation in resistant cancers. Thus, IKK and PI3K inhibitors may potentially be used therapeutically in resistant breast cancers that have increased expression of Bcl-2. We propose that the knockdown of resistance molecules STAT5 and Bcl2 along with the chemical inhibition of PI3K will have a synergetic effect promoting an increased apoptotic rate of the treated breast cancer cells than by inhibiting only PI3K and JAK2. Biography Daniel Díaz obtained his Biology BSc at the Universidad Juárez del Estado de Durango (México), he then obtained his PhD diploma at the Universidad Autónoma de Nuevo León where he was awarded with an Academic Excellence fellowship from the National Board for Science and Technology (CONACyT) and a fellowship granted by the Science and Technology Research Support Program (PAICyT). He had the opportunity of undergoing extensive training in the Medical Center (Houston, TX) under the tutelage of several researchers in the University of Texas and M.D. Anderson Cancer Center and in the Institute of Biotechnology in Madrid, Spain. Currently, he is a Post-Doctoral Fellow in the Faculty of Medicine of the Charles University at Prague, Czech Republic.

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 149

Taek Hwan Lee et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Inhibitory effects of resveratrol-enriched rice on ultraviolet B-induced pigmentation in guinea pig skin Taek Hwan Lee1, So-Hyeon Baek2, Jae Ok Seo3 ,HussainMustatab Wahedi3, Sun Yeou Kim3 1 Yonsei University, Republic of Korea 2 National Institute of Crop Science, Korea 3 Gachon University, Republic of Korea

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esveratrol is a polyphenolic compound found in several fruits and vegetables. Resveratrol has been shown to possess various health benefits such as anti-cancer, anti-hyperlipidemia, and anti-aging properties. Moreover, we previously reported skin whitening effect of resveratrol. Recently, many groups had attempted to create transgenic plants that accumulate resveratrol. Transgenic resveratrol-enriched rice was first developed by the Rural Development Administration of Korea, and we studied its inhibitory effects on ultraviolet B-induced skin hyperpigmentation. Our study shows that resveratrol-enriched rice significantly suppresses expression of tyrosinase, the predominant enzyme in melanogenesis, in mouse melanocytes (Melan-a cells). In addition, we studied whether topical application of resveratrol-enriched rice extract to the dorsal skin of brownish guinea pigs in vivo prevents ultraviolet B-induced hyperpigmentation. Our results show that resveratrol-enriched rice significantly suppresses expression of melanogenic proteins such as tyrosinase, TRP-1 and MITF in guinea pig skin. Histological study suggests that melanin production also decreased in the epidermis. Although rice is known to possess skin whitening effect, resveratrol-enriched rice was even more effective than itself. These results may be due to synergic effect between resveratrol and ingredients of rice. In further studies, we will investigate correlation of resveratrol and ingredients of rice. Biography Taek Hwan Lee finished his Masters in East-West Medical Sciences at the age of 27 from Kyung Hee University, Korea. He has been involved in pharmacognosy and neurosciences research. Currently he is doing Doctorate from Yonsei University, Korea. He has published 3 papers in international journals. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 150

Jun Cai, J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Discovery of structure-based small molecular inhibitor of αB-crystallin against basal-like/triple negative breast cancer development in vitro and in vivo Jun Cai Cardiff University School of Medicine, UK

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B-crystallin (CRYAB) is present at a high frequency in poor prognosis basal-like breast tumours, which are largely absent of oestrogen, progesterone receptors and HER2 known as triple-negative breast cancer (TNBC). CRYAB functions as a molecular chaperone to bind to and correct intracellular misfolded/unfolded proteins such as vascular endothelial growth factor (VEGF), preventing nonspecific protein aggregations under the influence of the tumour microenvironment stress and/or anticancer treatments including bevacizumab therapy. Directly targeting CRYAB can sensitize tumour cells to chemotherapeutic agents and decrease tumour aggressiveness. However, growing evidence shows that CRYAB is a critical adaptive response element after ischemic heart disease and stroke, implying that directly targeting CRYAB might cause serious unwanted sideeffects. Here, we used structure-based molecular docking of CRYAB and identified a potent small molecular inhibitor, NCI41356, which can strongly block the interaction between CRYAB and VEGF165. The disruption of the interaction between CRYAB and VEGF165 elicits in vitro anti-tumour cell proliferation and invasive effects through the down-regulation of VEGF signalling in the breast cancer cells. The observed in vitro anti-tumour angiogenesis of endothelial cells might be attributed to the down-regulation of paracrine VEGF signalling in the breast cancer cells after treatment with NCI-41356. One hundred micromolar intraperitoneal injection of NCI-41356 greatly inhibits the tumour growth and vasculature development in in vivo human breast cancer xenograft models. Our findings provide “proof-of-concept” for the development of highly specific structure-based alternative targeted therapy for the prevention and/or treatment of TNBC. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 151

May-Jywan Tsai et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Nigral mesenchymal stem cell graft and/or GDNF gene transfer promotes functional recovery in hemiparkinsonian rats May-Jywan Tsai1, Kang-Du Liu1, Su-Fen Fan1, Dann-Ying Liou1, Ching-Feng Weng2, Shih-Chieh Hung3 and Henrich Cheng1,3 1 Taipei Veterans General Hospital, Taiwan 2 National Dong-Hwa University, Taiwan 3 National Yang-Ming University, Taiwan

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arkinson’s disease (PD) is a neurological disorder characterized by the progressive loss of midbrain dopamine (DA) neurons. GDNF is known to exert neuroprotective and trophic effects on DA neurons. Bone marrow mesenchymal stem cells (MSC) represent potential cell sources for treating CNS injury. The present study aimed to evaluate the efficacy of nigral graft of human MSC and/or adenoviral (Ad) GDNF gene transfer in 6-OHDA-lesioned hemiparkinsonian rats. We used the immortalized human MSCs that retain their potential for neuronal differentiation. Pre-induced hMSC and/or Ad-GDNF were infused to the rat substantia nigra at 2 weeks after middle forebrain bundle infusion of 6-OHDA. Hemiparkinsonian rats receiving grafts of pre-induced hMSC or Ad-GDNF showed significant recovery in apomorphine- induced rotational behavior as well as numbers of nigral DA neurons. The result suggests that hMSC and GDNF have great potential in clinical cell therapy in Parkinsonian patients. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 152

Sarah Y Broeckx et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Skin-derived epithelial stem cells for wound regeneration in horses Sarah Y Broeckx, Hubert Berghs and Jan H Spaas Global Stem Cell Technology, Belgium

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esides the presence of somatic stem cells in hair follicles and dermis, the epidermis also contains a subpopulation of stem cells, reflecting its high regenerative capacity. However, only limited information concerning epidermis-derived epitheliallike stem/progenitor cells (EpSCs) is available to date. After harvesting from equine skin, the purified cells were characterized as EpSCs by means of positive expression for CD29, CD44, CD49f, CD90, Casein Kinase 2b, p63, and Ki67, low expression for cytokeratin (CK)14 and negative expression for CD105, CK18, Wide CK, and Pan CK. Furthermore, their self-renewal capacity was assessed in adhesion as well as in suspension. Moreover, the isolated cells were differentiated towards keratinocytes and adipocytes. To assess the regenerative capacities of EpSCs, full-thickness skin wounds were made in the horse as experimental animal model. The dermis of EpSC-treated wounds was significantly thinner and exhibited more restricted granulation tissue than did the control wounds. In addition, the EpSC-treated wounds also exhibited increases in EpSCs, vascularization, elastin content, and follicle-like structures. Biography Sarah Y Broeckx, Veterinarian, graduated from the Faculty of Veterinary Medicine, Ghent University (Belgium) in 2010. Then, she worked at the Department of Obstetrics, Reproduction and Heard Health of the Faculty of Veterinary Medicine, Ghent University. In 2012, she became the quality manager and regulatory consultant of Global Stem Cell Technology, an organization that is specialized in regenerative therapies for horses. Thanks to her contribution to equine stem cell research, she is an author of several scientific publications. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 153

M Monfrini et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Valuation of human mesenchymal stem cells (hMSC) effects on pancreatic islets M Monfrini1, E Donzelli1, V Rodriguez-Menendez1, B Bonandrini2, M Figliuzzi2, A Remuzzi3, G Tredici1 and A Scuteri1 1 Milano-Bicocca University, Italy 2 IRCCS- Istituto di Ricerche Farmacologiche Mario Negri, Italy 3 University of Bergamo, Italy

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he cell-based therapy is a promising approach to treat many degenerative diseases such as type 1 Diabetes Mellitus (T1DM). Besides the exclusive pharmacological treatment for T1DM a new approach has been recently proposed for restoring of Beta cell mass by islet transplantation. One of the principal problems of this approach is the numerical and functional loss of transplanted islets. For these reasons new strategies are studied in order to increase islets survival. In our laboratories we have already demonstrated that rat Mesenchymal Stem Cells (rMSC) are able to promote islets survival in vitro and that rMSC, if co-cultured with pancreatic islets, are able to express Pdx1, a gene involved in beta cell insulin secretion. The aim of this study is to verify the effect of human Mesenchymal Stem Cells (hMSC) on the survival and function of pancreatic islets. In order to clarify which mechanism could be involved in the putative positive effect we set up different culture conditions: Direct co-culture, in which hMSC were in direct contact with islets; indirect co-culture in which hMSC and islets shared the medium; mix cocultured in which islets were both in direct contact and shared the medium with hMSC. Preliminary results demonstrate a positive effect of hMSC on islets survival. Now we are focusing on the effect on insulin secretion regulated by hMSC in the different co-culture conditions. Biography M Monfrini is attending Neuroscience PhD program at Department of Surgery and Translational Medicine, Milano-Bicocca University. At present she is studying the protective effect of hMSC on different models of degenerative pathologies such us T1DM and peripheral nervous system degenerative diseases. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 154

Jyoti Bhojwani, J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Comparative expression profiling reveals critical temporal requirement of CKI and hDlg in developing colorectal carcinomas Jyoti Bhojwani Indore University, India

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patio-temporal cues defined for certain critical components in a particular developmental pathway provide a firm basis for detecting the order, hierarchy and “switching-off or on” of genes that regulate it. The various time-points at which genes are switched “on/off ” determines the fate of how a cell behaves in terms of being functional or non-functional, due to disruption of that specific pathway. This piece of work reports a strong evidence, toward identifying such components (associated with Wntsignaling) which indeed determine the transformation of a “blank-slate” (“cells of origin” and/or putative “cancer stem cells”) or “primitive-state” epithelial cells to an intermediate adenoma/polyp (dyspastic), and later to a proliferative (hyperplastic) or cancerous (neoplastic) state/s. The report discusses a critical temporal requirement of Caesin-Kinase I (CKI) and Human-Discslarge (hDlg), which have been identified as “early” and “late” acting molecules respectively, in a very crucial developmental event, that basically transforms “polyps” to full-fledged “carcinomas” (epithelial cancers) in Colorectal(CRC) tumors. A concomitant loss of CKI protein, in cells mutant for APC, a tumor suppressor gene, basically initiates the process of early-late polyposis, which furthers the progression of tumorigenesis, to finally give rise to confluent malignant cancers. The disturbance of architectural properties during metastasis, owing to the loss of apico-basal polarity of cells, consequently perturbs the hDlg protein expression, since it is basically a membrane associated protein, belonging to the MAGUK (Membrane-AssociatedGuanylate-Kinase) family. The loss of a critical cytoplasmic-component of APC protein, which most prominently disappears in cells mutant for APC, actually governs the early loss of expression of CKI during polyposis and consequently hDlg in later stages of colorectal carcinoma development. The detection of this vital parameter, served as a focal-point and the most striking diagnostic feature, for detection of effects, i.e., gain/ loss of other downstream components of Wnt-pathway, involved during the progression of CRC disease. The results presented here, prove that apart from being associated with each other as bindingpartners during Wnt-signaling, the loss of CKI and APC, together, is indeed responsible for triggering the normal epithelial cells of intestinal lining, to polyps followed by an awry late/confluent cancerous state, where hDlg expression also gets aberrant, due to subsequent loss of architectural properties in these abnormal cancer cells. Coincidentally, the chromosomes on which these genes reside have been found to be dense and rich in SNPs (hot-spots), the details of which have been recently discussed in a separate report. Biography Jyoti Bhojwani is presently a Faculty of Genetics/Bioinformatics/ Principal Investigator of the MTech Research Programs (Bio-Informatics) at University of Indore, India. She obtained her BSc (Bachelors degree) in Biological Sciences/Chemistry/Physics, MSc (Master’s degree) in Life-Sciences, and Doctoral degree (PhD) at School of Life-Sciences, University of Indore. She pursued her Post-doctoral ventures at Max-Planck Institute for Biophysical Chemistry (FRG), University of California-Irvine and University of Pittsburgh (USA). Currently, her projects mainly focus on translational-research and extrapolation of basic developmental mechanisms from model-systems like fruitfly (Drosophila) to human. She is keen on studying in details the genetic factors, which presumably aid in understanding of mechanism by which “cancer stem cells” function in transforming a tissue from normal to cancerous states. Her research has a motive to further facilitate the perception of stem cell potential/mechanistic in areas of regenerative medicine, translational research and anti-cancer therapy. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 155

Samaneh Khodadadi et al., J Stem Cell Res Ther 2014, 4:9 http://dx.doi.org/10.4172/2157-7633.S1.008

3rd International Conference and Exhibition on

Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Effect of silver nanoparticles and hydro alcoholic extract of Cucurbita pepo on burn injury Samaneh Khodadadi and Nooshin Naghsh Islamic Azad University, Iran

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ilver nanoparticles cause stress oxidative by free radicals production. Antioxidant agents could result in harmful of free radicals. The use of nanoparticles and natural compounds as a biologic cure is effective and increase healing. This study was performed to investigation of the silver nanoparticles effects on skin burn repair in male mice. We used different concentrations of silver nanoparticles and ethanol extracts of Cucurbita pepo in the burned area into nine groups (8 males/group) of control, positive control and other treatment groups. Wound healing was measured during treatment (28 day) and data analyzed by ANOVA test. At the end of treatment, samples with hematoxylin-eosin were stained. The group that received the combination of silver nanoparticle and pumpkin extract on the seventh day showed a significant increase in wound healing, but the pumpkin extract 50% in the entire course of treatment showed significantly less improvement but indicated the best quality of skin tissue. But in 2 groups of different dose of silver nanoparticles we observe epidermal cysts. These results proved that, the amount of antioxidants found in different doses of a plant are not the same so we suggest a new combination of silver nanoparticles and Cucurbita pepo as a new drug. This project is not over yet and our research is continuing in a large format. Biography Samaneh Khodadadi is an physiologist that has completed her Msc at the age of 25 years from Falavarjan University. She was chosen as the best presenter of the National Congress of Biological Sciences, Damghan, Semnan, Iran and received Young Researcher Award in Third National Congress of Trace Elements, Kashan Medical University, Isfahan, Iran. She has published three papers in scientific journals and has eleven oral and poster presentation in international and national congress. [email protected]

J Stem Cell Res Ther 2014 ISSN: 2157-7633, JSCRT an open access journal

Cell Therapy-2014

Volume 4, Issue 9

October 27-29, 2014

Page 156