BOROUGH OF MANHATTAN COMMUNITY COLLEGE City University of New York Department of Science Microbiology Bio420

Class Hours total 6

Lecture hours: 3

Laboratory Hours: 3

Semester: Spring 2014

Credits: 4

Instructor Information (Office number; Phone; Email; Office hours) Course Description BIO420 is an introductory microbiology class that covers the essentials of microbiology, interactions between microbes and the human host, and microbes and important human diseases. The course surveys microorganisms pathogenic to humans: their characteristics, pathogenicity and modes of transmission are studied. Laboratory instruction includes a study of the sterile technique and the culturing, staining and handling of bacteria. Prerequisites/Co-requisites BIO 426 and CHE 118 or CHE 121, or departmental approval Course student learning outcomes Students will be able to describe the characteristics of bacteria, viruses, prions and fungi. Students will be able to describe: DNA replication; protein synthesis; gene expression and mutation; genetic mechanisms of drug resistance. Students will be able to describe the mechanisms of host resistance and immunity.

Students will be able to identify the major bacterial, viral and fungal pathogens and understand the interaction between host & pathogen.

Students will be able to culture, stain, and handle bacteria with emphasis on safety and sterile technique.

Measurement Quizzes and examinations. Students will be assessed on their ability to differentiate between these organisms both structurally and functionally. Quizzes and examinations. In particular, students’ understanding of different types of mutations and the ways in which drug resistance arises will be assessed. Quizzes and examinations. Questions on the exam will assess students’ understanding of the interactions between different aspects of innate and adaptive immunity. Quizzes and examinations. Questions on the exam will include case-study approaches to assess students’ understanding of how important diseases are transmitted, the pathology of the diseases, and treatment of the diseases. Written laboratory reports and assignments. Improvements in clarity and organization of reports will be monitored. Students will also be given a practical exam in which they will be asked to (i) identify different staining techniques, (ii) describe techniques used for obtaining a pure cultures and (iii) understand the Kirby-Bauer method used for determining antibiotic sensitivity.

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Below are the college’s general education learning outcomes, the outcomes that are checked in the left-hand column indicate goals that will be covered and assessed in this course. (Check at least one.) General Education Learning Outcomes

Measurements (means of assessment for general education goals listed in first column)

Scientific Reasoning - students will be able to apply the concepts and methods of the natural sciences.

Students will assimilate class and laboratory information in order to answer questions related to biology orally and in written form.

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Required Lecture Text: Required Lecture Text:

Title: Authors: Publisher: Edition: ISBN #:

Microbiology: A Human Perspective Nester, Anderson and Roberts McGraw-Hill Publishing 7e, 2012 9781259116452

Required Laboratory Text: Title: Author: Publisher: Edition: ISBN #:

Microbiology, Laboratory Manual Penner McGraw-Hill Publishing 2e, 2008 Packaged with text

Other Resources 1. McGraw-Hill’s Connect Plus: To register, go to the web address provided by your instructor

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Click the “Register Now” button Enter your e-­­mail address Enter the Connect Plus Access Code found bound into your textbook

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Complete the Registration Form and click “Submit” For technical issues with registration, call 800-­­331-­­5094 or go to www.mhhe.com/support

2. Center for Disease Control website—Morbidity & Mortality Weekly Report (case studies) www.cdc.gov Evaluation & Requirements of Students Lecture Examinations Laboratory Examinations Other Assignments Final Examination

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LECTURE (NESTER TEXT) WEEK

TOPIC

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Humans and the microbial world Prokaryote cell structure Macromolecules

PAGES IN TEXT BOOK Required reading Chapter 1: 1-15 (introduction)* Chapter 3: 39 – 50*** (microscopy techniques) Chapter 3: 50 – 68 (prokaryote cell structure) Chapter 2: 25 – 29 (proteins) Chapter 2: 32 – 33 (nucleic acids) Chapter 2: 33 – 37 (lipids) * Your instructor may require supplementary reading from chapters 10 and 11 *** Optional pages – these may be covered in lab session by your instructor

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Chapter 2: 29 – 32 (carbohydrates) Chapter 4: 82 – 97 (microbial growth) Chapter 4: 104*** (methods to detect growth) Chapter 6: 126 – 148 (carbohydrate metabolism)

Microbial growth Microbial metabolism

*** Optional pages – these may be covered in lab session by your instructor 3

Chapter 7: 161 – 182 (transcription, translation, prokaryote gene control) Chapter 9: 215 – 235*** (biotechnology)

The blueprint of life: DNA to protein

*** Optional pages – your instructor may or may not cover this section 4

Bacterial genetics

Chapter 8: 188 – 212 (mutation, horizontal gene transfer)

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Viruses and prions

Chapter 13: 304 – 326 (viruses) Chapter 13: 328 – 330 (prions) Chapter 13: 313 – 314*** (filamentous phages) *** Optional pages – your instructor may or may not cover this section

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Innate immune response

Chapter 14: 334 – 351 (innate immunity)

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Adaptive immune response

Chapter 15: 354 – 375 (adaptive immunity) Chapter 15: 375 – 377*** (generation of diversity) *** Optional pages – your instructor may or may not cover this section 4

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Microbial mechanisms of pathogenicity (host-microbe interactions) Epidemiology

Chapter 16: 380 – 396 (host-microbe interactions) Chapter 19: 437 – 453 (epidemiology)

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Immunologic disorders Applications of immune responses

Chapter 17: 401 – 412 (immunologic disorders) Chapter 17: 412 – 416 (autoimmune disease and immune deficiencies) Chapter 19: 419 – 425 (immunization) Chapter 19: 426 – 434*** (immunologic testing) *** Your instructor will indicate what he/she plans to cover in these pages

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Chapter 5: 107 – 122 (control of growth) Chapter 20: 457 – 479** (antimicrobial drugs)

Control of microbial growth Antimicrobial medications

** Your instructor will indicate the level of detail to be covered 11

Chapter 21: 483 – 519 Chapter 22: 521 – 547

Respiratory infections Skin infections

* Your instructor will indicate which diseases are to be covered 12

Chapter 23: 548 – 569 Chapter 24: 571 – 609

Wound infections Digestive system infections

* Your instructor will indicate which diseases are to be covered 13

Chapter 25: 611 – 639 Chapter 26: 641 – 668

Genitourinary infections Nervous system infections

* Your instructor will indicate which diseases are to be covered 14

Blood and lymphatic infections HIV and complications of immunodeficiency

Chapter 27: 670 – 692 Chapter 28: 694 – 717 * Your instructor will indicate which diseases are to be covered

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FINAL EXAMS

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LABORATORY (ALL CLASSES) WEEK 1 2 3 4 5 6 7 8 9 10 11 12 13 14

TOPIC Light microscope Simple stain Gram stain/ Exposure plates Exposure plates/ Spore stain Acid fast stain/ Separation of a mixture Direct microscopic count Turbidity assay DNA electrophoresis Antibiotics/ disinfectants Water testing Culture of fungi Fungal morphology Pathogenic protozoa Lab exam

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College Attendance Policy At BMCC, the maximum number of absences is limited to one more hour than the number of hours a class meets in one week. For example, you may be enrolled in a three-hour class. In that class, you would be allowed 4 hours of absence (not 4 days). In the case of excessive absences, the instructor has the option to lower the grade or assign an F or WU grade. Academic Adjustments for Students with Disabilities Students with disabilities who require reasonable accommodations or academic adjustments for this course must contact the Office of Services for Students with Disabilities. BMCC is committed to providing equal access to all programs and curricula to all students. BMCC Policy on Plagiarism and Academic Integrity Statement Plagiarism is the presentation of someone else’s ideas, words or artistic, scientific, or technical work as one’s own creation. Using the idea or work of another is permissible only when the original author is identified. Paraphrasing and summarizing, as well as direct quotations, require citations to the original source. Plagiarism may be intentional or unintentional. Lack of dishonest intent does not necessarily absolve a student of responsibility for plagiarism. Students who are unsure how and when to provide documentation are advised to consult with their instructors. The library has guides designed to help students to appropriately identify a cited work. The full policy can be found on BMCC’s web side, www.bmcc.cuny.edu. For further information on integrity and behavior, please consult the college bulletin (also available online).

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