Bioanalytical & Bioprocess

Bioprocess & HCP ELISA Biosimilar Conformational ELISA Protein Refolding & Aggregation PEGylated & PEG Immunogenicity ELISA Interfering Protein Removal Kits Hybridoma Screening Kit Cell Cytotoxicity Assay Endotoxin Recovery, Detection & Removal Agarose Beads & Empty Columns Lipid Removal & Clarification

®

Optimize & Monitor Biologics Integrity

ProteoStat® Protein Aggregation Assay The ProteoStat® Protein Aggregation Assay is a simple, sensitive, homogeneous fluorescent microplate assay. The novel molecular rotor dye used detects a broad range of different protein aggregates from visible to subvisible particles. Ÿ Delivers superior results when compared to conventional protein detection dyes (Sypro Orange, Thioflavin T,

ANS, Congo Red) Ÿ High throughput method for monitoring protein stability when used with flow cytometry Ÿ Convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques Ÿ Useful assay to troubleshoot protein damage, from protein development through manufacturing stage

PRODUCT NAME

CAT. NO.

ProteoStat® Protein Aggregation Assay

ENZ-51023

ProteoStat® Protein Aggregation Standards

ENZ-51039

ProteoStat® Thermal Shift Stability Assay The ProteoStat® Thermal Shift Stability Assay is a rapid, simple screening method based on a novel molecular rotor dye which detects protein aggregation. Ÿ Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration Ÿ Screen for ligand binding to proteins of unknown function Ÿ Screen protein variants to determine their relative stabilities or excipients to determine optimal protein

storage conditions Ÿ Identify protein-protein interaction inhibitors

PRODUCT NAME

CAT. NO.

ProteoStat® Thermal Shift Stability Assay

ENZ-51027

ProteoStat® Protein Refolding & Aggregation Sensing Kit

ENZ-51040

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

Contamination Monitoring

®

Bioprocess & Host Cell Protein ELISA Kits

PRODUCT NAME

CAT. NO. ADI-900-057

Protein A ELISA Kit His-Tag ELISA Kit

AKR-130

The Host Cell Protein ELISA kits and reagents are ideal for impurity analysis, Host Cell Protein quantitation and Western blots.

GFP ELISA Kit

AKR-121

E. coli Host Cell Protein ELISA Kit*

ENZ-KIT127

Ÿ Quantitatively measures host cell protein

CHO Host Cell Protein ELISA Kit*

ENZ-KIT128

contamination in E.Coli and CHO derived biologics Ÿ High throughput format with results in 2.5 hours

Protein A ELISA Kit

Most Sensitive 9.01 pg/ml

The Protein A ELISA kit is a sensitive and reproducible sandwich assay to quantify Protein A residuals in monoclonal antibody preparations. This extensively validated ELISA kit enables efficient detection of natural and recombinant Protein A constructs with up to 100% recovery.

Bovine Albumin ELISA Kit

E11-113

Bovine IgA ELISA Kit

E11-121

Bovine IgG ELISA Kit

E11-118

Bovine IgM ELISA Kit

E11-101

Bovine Transferrin ELISA Kit

E10-122

Bovine Lactoferin ELISA Kit

E11-122

Human Albumin ELISA Kit

E88-129

Human IgA ELISA Kit

E88-102

Human IgG ELISA Kit

E88-104

Human IgM ELISA Kit

E88-100

Human Transferrin ELISA Kit

E88-128

Ÿ Detects 1ppm of Protein A residuals in human IgG Ÿ Recognizes 4 different Protein A variants Ÿ Useful for contamination analysis and

measurement of Protein A variants in monoclonal antibody preparations Ÿ Produces results in less than 3 hours with low cost per test

*Manufactured under cGMP

Antibodies & Protein Standards to detect HCPs using Western blot analysis PRODUCT NAME

Protein A (Sa)

Recombinant Protein A (Ec)

Millipore

Natural

Repligen

Recombinant Cys-Protein A (Ec) GE

Recombinant alkaline-resistant Protein A (Ec)

HCF1 (hamster), pAb

ENZ-ABS260-0100

HCF1 (hamster), pAb (biotin conjugate)

ENZ-ABS261-0100

HCF1 (E. coli), pAb

ENZ-ABS262-0100

HCF1 (E. coli), pAb (biotin conjugate)

ENZ-ABS263-0100

IgG (human), pAb

ENZ-ABS264-1000

IgG (human), pAb (biotin conjugate)

ENZ-ABS265-1000

E. coli Host Cell Protein ELISA Standards

ENZ-PRT121-0050

CHO Host Cell Protein ELISA Standards

ENZ-PRT122-0050

E.coli HCP Chemiluminescent WB kit

KCAJ07

GE MabSelet SuRe

Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery calculated by dividing observed concentration by expected concentration. A: Natural Protein A from S. aureus (Millipore), n=9; B: Recombinant Protein A from E. coli (Repligen), n=9; C: Recombinant CysProtein A from E. coli (GE), n=12; and D: Recombinant alkaline-resistant Protein A variant from E. coli (MabSelet SuRe from GE), n=12; graphical data represents statistical mean +/- 1 standard deviation. Ref.: Enzo Life Sciences

Phone : +91-11-4875-4875

CAT. NO.

Email : [email protected]

Fax : +91-11-2561-2008

Improved Screening for Biotherapeutics

®

Reduce Hybridoma Screening & Cloning Time by 75%

Magnetic Bead Separation Hybridomas produce secreted antibody AND surface-bound antibody

TM

The DiSH Kit is a unique technology for the rapid production of hybridomas. Using a genetically-modified myeloma partner, the kit creates hybridomas capable of both secreting and surface-expressing antigen-specific antibodies, enabling screening and selection of positive clones by FACS. The components of the kit are available as standalone products as well.

Enrich cells with Antigen-coupled Magnetic beads

OR

Label cells with Fluorescent Antigen

PRODUCT NAME

specific, stable hybridomas Ÿ Minimizes manipulation of cloned cell lines

relative to approaches requiring Limiting Dilution Subcloning (LDS) Ÿ Decreases time and labor costs

TM

ENZ-71001

AbeoCloneTM (Base Semi-solid Medium)

ENZ-70009

AbeoCloneTM (HAT Semi-solid Medium)

ENZ-70004

AbeoCloneTM (CHO TCSC Semi-solid Medium)

ENZ-70010

The BioSimsTM Conformational ELISA kits allow for 3-D conformational comparison, higher-order structure (HOS) characterization, between biosimilars and marketed mAb. This new technology provides a sensitive, systematic, and robust measurement of biosimilar conformation comparability at the molecular level. Ÿ Unique measurement of biosimilar mAb HOS Comparability Ÿ Detects small amounts of conformational impurity, ≥ 0.1% Ÿ Correlates well with bioassays and glycosylation analysis Steptavidin

NH

Horse Radish Peroxidase

Oxidized TMB

Biotinylated

Anti-human IgG

NH2

H 2N

Antibody

TMB

Therapeutic

Protein

Anti-peptide Antibody

PRODUCT NAME

PEGylated Protein ELISA Kit The sensitive PEGylated Protein ELISA kit is ideal for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and inprocess QC studies. Ÿ Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins Ÿ Sensitive assay measures < 1ng/ml of PEGylated molecules Ÿ Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue Sensitive detection of linear and branched PEG molecules

CAT. NO.

Adalimumab Conformational ELISA

ENZ-KIT122

Alemtuzumab Conformational ELISA

ENZ-KIT119

Bevacizumab Conformational ELISA

ENZ-KIT118

Cetuximab Conformational ELISA

ENZ-KIT120

Conserved mAb Sequence Conformational ELISA

ENZ-KIT123

Infliximab Conformational ELISA

ENZ-KIT124

Palivizumab Conformational ELISA

ENZ-KIT126

Rituximab Conformational ELISA

ENZ-KIT125

Trastuzumab Conformational ELISA

ENZ-KIT121

Phone : +91-11-4875-4875

CAT. NO.

DiSHTM Kit

Conformational ELISA Kits

HN

FACS: Fluorescence-Activated Cell Sorting Ag

Ÿ Enables faster, improved selection of

BioSims

Ag

PRODUCT NAME PEGylated Protein ELISA Kit

Email : [email protected]

CAT. NO. ADI-900-213

Fax : +91-11-2561-2008

® Improved Screening for Biotherapeutics Improved Screening for Biotherapeutics

NIDS® PEG Immunogenicity ELISA Recognizes PEG polymers of various sizes

The NIDS® PEG Immunogenicity ELISA provides a complete set of reagents to detect PEG-specific antibodies in human serum and other matrices.

Depletion with 20 kDa PEG 1 0.9

0.8

•

H-40 kDa PEG

0.5 0.4

0.2 0.1 0 0.01

0.1

1

10

100

1000

10000

Molar Ratio PEG: Ab

Depletion with 30 kDa PEG 0.9 0.8 0.7

0.6 0.5 0.4

0.3 0.2 0.1

40 kDa PEG

•

0.6

0.3

A 450/630

Y

• •

Double Antigen Bridging Assay in 96-well format Extensively tested with human serum samples 2.5 hour turnaround time Detects Abs to PEGs of various lengths and with different linking groups PEG Precoated plates Anti-H-HRP conjugate Colorimetric, HRP HRP based H ANPEG-1 Limit of Detection: 125 ng/mL Ab No matrix effects observed

A 450/630

0.7

• • • •

0

0.1

1

10

100

1000

Molar Ratio PEG: Ab

Depletion with 40 kDa PEG 0.9 0.8

PRODUCT NAME

CAT. NO.

0.7

NIDS® Polyethylene Glycol (PEG) Immunogenicity ELISA Kit

11-1035-1KT

NIDS® DIY Immunogenicity ELISA Kit (10 Plates)

90-1030-10EA

A 450/630

0.6 0.5 0.4

0.3

NIDS® PEG Immunogenicity Rapid Assay Kit (100 Tests)

1L-026-1KT

0.2 0.1 0

0.10

1.00

10.00

100.00

1000.00

10000.00

Molar Ratio PEG: Ab

NIDS® Streptavidin and Neutravidin Coated Plates The Streptavidin or Neutravidin-coated ELISA plates are often used when coated proteins are in limited quantities or tend to be denatured during a direct coating process. NIDS® HyperBind High Sensitivity and High Binding streptavidin- and neutravidin-coated ELISA plates are available in clear, black, and white formats for colorimetric, fluorescence and chemiluminescence-based assays • • • • • •

High Efficiency Coating Ready to use plates, pre-blocked with BSA Specific: High signal to noise ratios with low background Sensitive: Detection of < 1 ng/mL of protein is easily attainable Long shelf life: > 18 months at room temperature Low cost: Reduce reagent use by as much as 90%

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

Improved Screening for Biotherapeutics

®

Most sensitive streptavidin or neutravidin-coated ELISA plates for Serological Assays* Comparison of TSH Assays on HyperBind and Commercial Streptavidin Plates

ANP

Pierce HBC

Pierce

Nunc

R&D systems

Sandwich Assay Performance at Optimal Coating Concentrations

Binding Efficiency: HyperBind Streptavidin (SA) clear plates vs. commercial plates

Vendor 1

Vendor 2

HyperBind

Vendor 3

HyperBind NA plate,0.5μg/mL capture Ab

Vendor 1 NA plate, 5.0μg/mL capture Ab

Sandwich ELISA for mouse IgG using NIDS® HyperBind streptavidin (SA) and neutravidin (NA) plates and the commercially available Pierce SA and NA plates. NIDS® HyperBind plates were coated at 0.5 μg/mL capture Ab and the Pierce plates at 5.0 μg/mL. NIDS® HyperBind plates generate greater signal and sensitivity while using only 10% of the antibody required by the commercial plates. *(Reference: Vallejo YR, Li J, Yin R. Enhancing assay performance using nanoscale detection. IVD Technology, Volume 16, May/June 2010)

PRODUCT NAME

CAT. NO.

High Sensitivity Plates NIDS® HyperBind High Sensitivity Streptavidin Coated Clear Solid Plates (96 Well) (5 Plates)

11-1001-5PK

NIDS® HyperBind High Sensitivity Neutravidin Coated Clear Solid Plates (96 Well) (5 Plates)

11-1007-5PK

NIDS® HyperBind High Sensitivity Streptavidin Coated Black Solid Plates (96 Well) (5 Plates)

12-1001-5PK

NIDS® HyperBind High Sensitivity Streptavidin Coated White Solid Plates (96 Well) (5 Plates)

13-1001-5PK

NIDS® HyperBind High Sensitivity Streptavidin Coated Clear Stripwell Plates (96 Well) (300 plates)

21-1001-5PK

NIDS® HyperBind High Sensitivity Neutravidin Coated Clear Stripwell Plates (96 Well) (300 plates)

21-1007-5PK

NIDS® HyperBind High Sensitivity Streptavidin Coated Black Stripwell Plates (96 Well) (300 plates)

22-1001-5PK

NIDS® HyperBind High Sensitivity Neutravidin Coated Black Stripwell Plates (96 Well) (300 plates)

22-1007-5PK

NIDS® HyperBind High Sensitivity Streptavidin Coated White Stripwell Plates (96 Well) (300 plates)

23-1001-5PK

NIDS® HyperBind High Sensitivity Neutravidin Coated White Stripwell Plates (96 Well) (300 plates)

23-1007-5PK

High Binding Plates NIDS® HyperBind High Binding Streptavidin Coated Clear Solid Plates (96 Well) (5 Plates)

11-3001-5PK

NIDS® HyperBind High Binding Neutravidin Coated Clear Solid Plates (96 Well) (5 Plates)

11-3007-5PK

NIDS® HyperBind High-Binding Neutravidin Coated Black Solid Plate (96-Well) (5 Plates)

12-3007-5PK

NIDS® HyperBind High Binding Streptavidin Coated Clear Stripwell Plates (96 Well) (300 plates)

21-3001-5PK

NIDS® HyperBind High Binding Neutravidin Coated Clear Stripwell Plates (96 Well) (300 plates)

21-3007-5PK

NIDS® HyperBind High Binding Streptavidin Coated Black Stripwell Plates (96 Well) (300 plates)

22-3001-5PK

NIDS® HyperBind High-Binding Neutravidin Coated Black Stripwell Plate 96-Well (300 plates)

22-3007-5PK

NIDS® HyperBind High Binding Streptavidin Coated White Stripwell Plates (96 Well) (300 plates)

23-3001-5PK

NIDS® HyperBind High-Binding Neutravidin Coated White Stripwell Plate (96-Well) (300 plates)

23-3007-5PK

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

Improved Screening for Biotherapeutics

®

NIDS® DIY Biotinylation and Digoxigenin Labeling Kits NIDS® DIY Biotinylation and Digoxigenin labeling Kits provide an easy way to label peptides and proteins rapidly and efficiently. • • • •

Successfully used in PK and Immunogenicity Assays Short and long linkers are available Rapid QC test strip confirms successful labeling. Compared to other methods, which often require at least 100 µg of the conjugate, our QC procedure consumes only low nanograms of the labeled reagent.

Rapid QC Test Strips provided with the kit for confirmation of successful labeling of proteins

Sandwich Pharmacokinetics Assay

PRODUCT NAME

Sandwich Immunogenicity Assay

ANTIBODY/PROTEIN AMOUNT

CAT. NO.

NIDS® DIY Biotin-1500-NHS Antibody Labeling Kit

Up to 2 mg

90-1018-1KT

NIDS® DIY Biotin-1500-NHS Antibody Labeling Kit

Up to 20 mg

90-1018-10KT

NIDS® DIY Biotin-1500-NHS Protein Labeling Kit

Up to 2 mg

90-1017-1KT

NIDS® DIY Biotin-1500-NHS Protein Labeling Kit

Up to 20 mg

90-1017-10KT

NIDS® DIY Digoxigenin-NHS Antibody Labeling Kit

Up to 2 mg

90-1024-1KT

NIDS® DIY Digoxigenin-NHS Antibody Labeling Kit

Up to 20 mg

90-1024-10KT

NIDS® DIY Digoxigenin-NHS Protein Labeling Kit

Up to 2 mg

90-1023-1KT

NIDS® DIY Digoxigenin-NHS Protein Labeling Kit

Up to 20 mg

90-1023-10KT

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Improved Screening for Biotherapeutics

NIDS® NPX4000 Interfering Protein Removal Kits A simple and easy-to-use kit capable of irreversible removal of interfering endogenous proteins such as drug target compounds, which can cause erroneous results in immunogenicity and PK assays • Irreversibly removes >99% of endogenous interfering protein or drug target in patient samples • Highly efficient & does not affect original ADA levels • Simple extraction procedure easily adaptable to high throughput testing • Significantly greater capacity than microparticles • Capable of removing up to 400 ng/mL of interfering proteins • Uses up to 100-fold less capture reagent than microparticles • Suitable for all bioanalytical methods including PK and IM assays Use this kit to attach the specific blocking antibody or receptors to activated nanoparticles and pretreating the sample, followed by a centrifugation step, enables irreversible removal of interference without affecting the dection of ADAs

NPX4000 nanoparticles with scavenger/capture reagent Interfering protein

Y

Y

YY Y

YY YY Before Extraction

Centrifugation

Serum Sample

NPX4000 Particles

Y

After Extraction

Signal

Effect of Removal of Target Interference on an Immunogenicity Assay For a Biotherapeutic Monoclonal Antibody Using NPX4000 Extraction Particles

0

1

10

100

0

1

10

100

[Interfering Protein] ng/mL

PRODUCT NAME NIDS® NPX4000 Interfering Protein Extraction Kit (100 samples) (Generic DIY Kit)

Phone : +91-11-4875-4875

Email : [email protected]

CAT. NO. 90-1026

Fax : +91-11-2561-2008

Improved Screening for Biotherapeutics

®

List of 140 target proteins which can be extracted from test samples using specific antibodies. We can also prepare the specific extraction nanoparticle kit for your unique requirements. NIDS® NPX4000 INTERFERING PROTEIN REMOVAL KITS PRODUCT NAME

CAT. NO.

PRODUCT NAME

CAT. NO.

PRODUCT NAME

CAT. NO.

PRODUCT NAME

CAT. NO.

Acrp30

90-1095

FGF-4

90-1115

IL-12 p70

90-1147

MIP-3B

90-1179

AgRP

90-1096

FGF-6

90-1116

IL-13

90-1148

MSP-A

90-1180

Amphiregulin

90-1097

FGF-7

90-1117

IL-15

90-1149

NAP-2

90-1181

ANG

90-1098

FGF-9

90-1118

IL-16

90-1150

NT-3

90-1182

Angiopoietin-1

90-1099

Flt-3 ligand

90-1119

IL-17

90-1151

NT-4

90-1183

AXL

90-1100

Fractalkine

90-1120

IL-1A

90-1152

OPG

90-1184

BDNF

90-1101

GCP-2

90-1121

IL-1B

90-1153

OSM

90-1185

bFGF

90-1102

GCSF

90-1122

IL-1RA

90-1154

PARC

90-1186

BLC

90-1103

GDNF

90-1123

IL-2

90-1155

PCSK9

90-1187

BMP-4

90-1104

GITR

90-1124

IL-3

90-1156

PDGF-BB

90-1188

BMP-6

90-1105

GITR-ligand

90-1125

IL-4

90-1157

PIGF

90-1189

b-NGF

90-1106

GM-CSF

90-1126

IL-5

90-1158

RANTES

90-1190

BTC

90-1107

GRO-A

90-1127

IL-6

90-1159

RF

90-1191

CCL28

90-1108

HCC-4

90-1128

IL-6R

90-1160

SCF

90-1192

CD28

90-1109

HER2neu receptor

90-1129

IL-7

90-1161

SDF-1

90-1193

CD3 epsilon

90-1110

HGF

90-1130

IL-8

90-1162

sgp130

90-1194

CD39/ENTPD1

90-1111

hGH

90-1131

I-TAC

90-1163

TARC

90-1195

CD4

90-1112

I-309

90-1132

Leptin

90-1164

TECK

90-1196

CD5

90-1113

ICAM-1

90-1133

LIGHT

90-1165

TGF-beta 1

90-1197

Ck beta 8-1

90-1114

ICAM-3

90-1134

Lymphotactin

90-1166

TIMP-1

90-1198

CNTF

90-1083

IFN-gamma

90-1135

MCP-1

90-1167

TIMP-2

90-1199

CTACK

90-1084

IFN ß-1a

90-1136

MCP-2

90-1168

TNF RI

90-1200

Dtk

90-1085

IGF-1

90-1137

MCP-3

90-1169

TNF RII

90-1201

EGF

90-1086

IGF-1 SR

90-1138

MCP-4

90-1170

TNF-a

90-1202

EGF-R

90-1087

IGFBP-1

90-1139

M-CSF

90-1171

TNF-b

90-1203

ENA-78

90-1088

IGFBP-2

90-1140

MDC

90-1172

TPO

90-1204

Eotaxin

90-1089

IGFBP-3

90-1141

MIF

90-1173

TRAIL R3

90-1205

Eotaxin-2

90-1090

IGFBP-4

90-1142

MIG

90-1174

TRAIL R4

90-1206

Eotaxin-3

90-1091

IGFBP-6

90-1143

MIP-1A

90-1175

TrkA

90-1207

EPO

90-1092

IL-10

90-1144

MIP-1B

90-1176

uPAR

90-1208

ErbB2

90-1093

IL-11

90-1145

MIP-1D

90-1177

VEGF

90-1209

Fas

90-1094

IL-12 p40

90-1146

MIP-3A

90-1178

VEGF-D

90-1210

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Improved Screening for Biotherapeutics

aCellaTM-TOX - Bioluminescence non-radioactive cytotoxicity Assay A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells. aCellaTM-TOX use the same general ADCC and CDC processes where target cell apoptosis is induced. This lysis leads to the release of endogenous GAPDH, an enzyme in glycolysis and gluconeogenesis pathways, from the cytoplasm. Through the addition of coupling enzymes, adenosine triphosphate (ATP) is generated, and when coupled to a luciferase/luciferin reaction, produces a measureable bioluminescent signal. This signal is proportional to the amount of GAPDH release and target cell lysis. aCellaTM-TOX assay principle

Specifications & Advantages Versatile - Useful for measuring activity of T Cells, Primary Cells, NK, complement and other lytic agents. Assay can be run in serum supplemented media. FAST - Results in 3-5 minutes. Chromium 51 or europium release for measurement are time consuming. The inherent sensitivity of luciferase detection is enhanced by the amplification effect of enzyme turnover, which produces thousands, millions or billions of high - energy molecules for each molecule of the enzyme. Highly Sensitive - Can detect fewer than 500 cells/well in the presence of serum or as few as 10 cells/well in serumfree or heat killed media. GAPDH- The fact that GAPDH is a natural component of cells, and does not need to be introduced into the cells in any manner, distinguishes this assay from all methods which require prelabelling of cells, transfection, transformation, or other methods of introducing proteins or other molecules into the target cells in order to generate a signal in a later step. Advantages for measurement of cell mediated or complement mediated cytolysis - It is usually desirable to use smaller numbers of TCells than are needed for the 51Cr – release assay, since excessive numbers of effector cells can increase the background signal. This is now possible due to the high sensitivity of aCellaTM-TOX.

PRODUCT NAME

TEST

CAT. NO.

Non Radioactive Cytotoxicity Assay - aCellaTM-TOX

500

CLATOX100-3

Non Radioactive Cytotoxicity Assay - aCella -TOX (5 Lumi Plates)

500

CLATOX100-3L

Non Radioactive Cytotoxicity Assay - aCellaTM-TOX (5 Lumi Plates + 5 Tissue Culture Plates)

500

CLATOX100-3P

Non Radioactive Cytotoxicity Assay - aCellaTM-TOX

1000

CLATOX100-4

TM

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Solutions for Bioprocess Optimization

OXIDATIVE STRESS

CELL DEATH

Simplify your cell death analysis Cyto-ID® Autophagy Detection Kit (ENZ-51031)

GFP-CertifiedTM Apoptosis/Necrosis Detection Kit (ENZ-51002)

• No transfection required • Protocol validated with known inhibitors and activators • Rapidly quantifies autophagy in native heterogeneous cell populations • Negligible staining of lysosomes reducing background

• True multiplexing capabilities with GFP and other green fluorescent probes • Readily distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells • Optimized for both fluorescence microscopy and flow cytometry

Sensitive assays for oxidative stress Cyto-ID® Hypoxia/Oxidative Stress Detection Kit (ENZ-51042)

Mito-ID® Membrane Potential Cytotoxicity Assay Kit (ENZ-51019)

• Sensitive hypoxia dye fluoresces red when converted by nitroreductates • Total ROS dye enables simultaneous detection of hypoxia (red) and ROS (green) • Non-toxic, cell permeable dye optimized to avoid artifactual fluorescence • Suitable for microscopic or flow cytometry analysis of adherent or suspension cells

• 10x more sensitive than JC-1 with superior aqueous solubility • Photostable dual-emission dye • Separate Mito-ID® Red/Green assays available for detection of mitochondrial mass • Suitable for high-throughput applications

High-throughput analysis of mitotoxicity

SMALL MOLECULES

Bioactives for Drug Development Ultra-high Purity | Cost effective | Extensively Validated PRODUCT NAME

CAT. NO.

Compound Libraries

PRODUCT NAME

CAT. NO.

Autophagy Modulators 3-Methyladenine

BML-AP502

LY 294002

BML-ST420

Rapamycin

BML-A275

Wortmannin

BML-ST415

Osmotic Stress Inhibitors

BML-2837

JX-401

BML-EI366

BML-2850

PD 169,316

ALX-270-258

Hepatotoxicity Library

BML-2851

SB202190

BML-EI294

Hematopoietic Toxicity Library

BML-2852

SB220025

ALX-270-325

Autophagy Library Cardiotoxicity Library

ICCB Known Bioactives Library

BML-2840

FDA Approved Drug Library V2

BML-2843

Bioactive Lipid Library

BML-2800

Protease Inhibitor Library

BML-2833

Nephrotoxicity Library

ENZ-LIB100

Phone : +91-11-4875-4875

Protease Inhibitors Protease Inhibitor Cocktail

BML-KI103

3,4-Dichloroisocoumarin

BML-PI110

Actinonin

ALX-260-128

Chymostatin

ALX-260-005

Cystatin C (human)

ALX-850-292

Email : [email protected]

Fax : +91-11-2561-2008

Quality & Purity of Biotherapeutics

®

Cleanascite™ - Lipid adsorption and clarification reagent Cleanascite™ selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from Cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification. The reagent is a solid-phase, non-ionic adsorbent supplied as a suspension in saline, ready for use. Simply add, centrifuge and/or filter. The clarified supernatant is ready for subsequent downstream processing or analysis. Ÿ

A high binding capacity for lipids with minimal cross-reactivity with proteins

Ÿ

Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon) and it is environmentally friendly.

Ÿ

Very low protein binding

Ÿ

Does not bind to DNA, RNA, enzymes and proteins

Ÿ

Extends the life of membrane and chromatographic columns.

Ideal reagent for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.

TM

Treatement With Cleanascite

Clarifies:5

• Cohn Paste

• Ascites

3

• Transgenic Milk

• Cell Lysates

2

6

• Serum/Plasma

• Tissue Culture

• Organ Homogenates

• Egg Yolk

• Saliva/Sputum

4

Egg Yolk After (Left) and Before (Right) Insert: PAGE showing Left: Markers Right: IgY and other major protein fraction recovered

7

• Bile

PRODUCT NAME

SIZE

TOTAL SAMPLE VOLUME THAT CAN BE PROCESSED*

CAT. NO.

Cleanascite™

100 ml

400 ml

X2555 -100

Cleanascite™

1 Liter

4 Liters

X2555- 1000

Cleanascite™

4 Liters

16 Liters

X2555- 4000

Cleanascite™

16 Liters

64 Liters

X2555- 16000

*Based on Cleanascite™ to Sample typical volume ratio. Volume ratio may be adjusted according to lipid levels.

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

Agarose Beads for purification of Proteins We offers a wide range of activated agarose beads for affinity chromatography and enzyme and antibody immobilization processes, as well as a variety of non-activated agarose beads for size exclusion chromatography.

Compatible with TM AKTA & FPLCTM

AFFINITY CHROMATOGRAPHY PURIFICATION PRODUCT NAME

CAT. NO.

PACK SIZE

HIS-TAG PURIFICATION LOW PRESSURE: CHELATING AGAROSE BEADS: BULK RESINS

HIGH Density METAL FREE

HIGH Density NICKEL

HIGH Density ZINC

HIGH Density COBALT

LOW Density METAL FREE

LOW Density NICKEL

LOW Density ZINC

LOW Density COPPER

HIGH DENSITY CHELATE KIT + 40 empty mini columns

6BCL-KHSP-2 6BCL-KL-2

LOW DENSITY CHELATE KIT

6BCL-QH-100

100 ml

6BCL-QH-500

500 ml

LOW DENSITY CHELATE KIT + 40 empty mini columns

6BCL-QHNi-25

25 ml

6BCL-QHNi-100

100 ml

6BCL-QHNi-500

500 ml

6BCL-QHZn-25

25 ml

6BCL-QHZn-100

100 ml

6BCL-QHZn-500

500 ml

6BCL-QHCo-25

25 ml

6BCL-QHCo-100

100 ml

6BCL-QHCo-500

PACK SIZE

CAT. NO.

LOW PRESSURE: GLUTATHIONE AGAROSE BEADS: BULK RESINS GLUTATHIONE Agarose Beads

6BCL-KLSP-2

6BCL-NTANi-25

PACK SIZE

GST PURIFICATION

LOW PRESSURE: NTA AGAROSE BEADS: BULK RESINS

NICKEL NTA Agarose Resin

PRODUCT NAME

25 ml

6BCL-NTANi-100 100 ml

4B-GLU-10

10 ml

4B-GLU-100

100 ml

ANTIBODY PURIFICATION LOW PRESSURE: PROTEIN A AGAROSE BEADS: BULK RESINS

6BCL-NTANi-500 500 ml PROTEIN A Agarose Resin HIGH PRESSURE: CHELATING RAPID RUN™ BEADS: BULK RESINS

PA09-R5

5 ml

PA09-R25

25 ml

PA09-R500

500 ml

6NiRR-5

5 ml

6NiRR-25

25 ml

LOW PRESSURE: PROTEIN A AGAROSE BEADS: PRE-PACKED COLUMN

6NiRR-100

100 ml

PROTEIN A Agarose Column

PA09-C1-1

1 x 1 ml

500 ml

6NiRR-500

500 ml

PROTEIN A Agarose XL Column

PA09-C5-1

1 x 5 ml

6BCL-QL-25

25 ml

6CoRR-5

5 ml

PROTEIN A Test Kit

PA09-K-01

1 X 100 µl

6BCL-QL-100

100 ml

6CoRR-25

25 ml

6BCL-QL-500

500 ml

6CoRR-100

100 ml

6BCL-QLNi-25

25 ml

6CoRR-500

500 ml

6BCL-QLNi-100

100 ml

6BCL-QLNi-500

500 ml

His-COLUMN HIGH Density NICKEL

6BCL-QHNi-C8

8 x 1 ml

6BCL-QLZn-25

25 ml

HisXL-COLUMN HIGH Density NICKEL

6BCL-QHNi-C5

5 x 5 ml

6BCL-QLZn-100

100 ml

His-COLUMN HIGH Density ZINC

6BCL-QHZn-C8

8 x 1 ml

6BCL-QLZn-500

500 ml

HisXL-COLUMN HIGH Density ZINC

6BCL-QHZn-C5

5 x 5 ml

6BCL-QLCu-25

25 ml

6BCL-QLCu-100

100 ml

HisXL-COLUMN HIGH Density COBALT

6BCL-QHCo-C5

5 x 5 ml

6BCL-QLCu-500

500 ml

His-COLUMN LOW Density NICKEL

6BCL-QLNi-C8

8 x 1 ml

MABRRF-R10

10 ml

HisXL-COLUMN LOW Density NICKEL

6BCL-QLNi-C5

5 x 5 ml

MABRRF-R25

25 ml

His-COLUMN LOW Density ZINC

6BCL-QLZn-C8

8 x 1 ml

MABRRF-R100

100 ml

HisXL-COLUMN LOW Density ZINC

6BCL-QLZn-C5

5 x 5 ml

MABRRF-R200

200 ml

His-COLUMN LOW Density COPPER

6BCL-QLCu-C8

8 x 1 ml

MABRRF-K-01

1 X 100 µl

HisXL-COLUMN LOW Density COPPER

6BCL-QLCu-C5

5 x 5 ml

NICKEL CHELATE KIT + 20 empty mini columns

6BCL-KNiSP-2 6BCL-KNiCo-2

NICKEL & COBALT CHELATE KIT + 30 empty mini columns

6BCL-KNiCoSP-2

ZINC CHELATE KIT

6BCL-KZn-2

ZINC CHELATE KIT + 20 empty mini columns

6BCL-KZnSP-2

ZINC & COPPER CHELATE KIT + 30 empty mini columns

6BCL-KH-2

25 ml

6BCL-KNi-2

ZINC & COPPER CHELATE KIT

CAT. N O.

HIGH DENSITY CHELATE KIT

6BCL-QH-25

NICKEL CHELATE KIT

NICKEL & COBALT CHELATE KIT

PRODUCT NAME

NICKEL RAPID RUN™

COBALT RAPID RUN™

Phone : +91-11-4875-4875

PROTEIN L Agarose Resin

HIGH PRESSURE: RAPID RUN™ AGAROSE BEADS: PRE-PACKED COLUMNS

His-COLUMN HIGH Density COBALT

6BCL-QHCo-C8

PROTEIN L Test Kit

PROTEIN A Rapid RunTM Cartridges

8 x 1 ml

NICKEL RAPID RUN™ Cartridges 1ml

NICKEL Affinity Cartridges 5ml

6RRFNi-Ctg1-2

2 x 1ml

6RRFNi-Ctg1-5

5 x 1ml

AF6Ni-Ctg5-1

1 x 5ml

AF6Ni-Ctg5-5

5 x 5ml

Email : [email protected]

4BCLPL-R2

2 ml

4BCLPL-R5

5 ml

4BCLPL-R10

10 ml

4BCLPL-K-01

1 X 100 µl

HIGH PRESSURE: PROTEIN A RAPID RUN BEADS: PRE-PACKED CARTRIDGE

6RRFPA-Ctg1-2

2 x 1 ml

6RRFPA-Ctg1-5

5 x 1 ml

TM

HIGH PRESSURE: AFFI-MAB BEADS: BULK RESINS

AFFI-MABTM Fine Agarose Resin

AFFI-MABTM Fine Test Kit

HIGH PRESSURE: PROTEIN G RAPID RUNTM BEADS: BULK RESINS

HIGH PRESSURE: CHELATING RAPID RUN™ BEADS: PRE-PACKED CARTRIDGE

6BCL-KZnCu-2 6BCL-KZnCuSP-2

LOW PRESSURE: PROTEIN L AGAROSE BEADS: BULK RESINS

PROTEIN G Agarose Resin 4 RAPID RUN™ PROTEIN G Test Kit

4RRPG-1

1 ml

4RRPG-5

5 ml

4RRPG-25

25 ml

4RRPG-K-01

1 X 100 µl

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

SIZE EXCLUSION CHROMATOGRAPHY PRODUCT NAME

CAT. NO.

PACK SIZE

PRODUCT NAME

CAT. NO.

LOW PRESSURE: PLAIN AGAROSE BEADS

2% B Agarose Bead STANDARD (50-150µm)

4% B Agarose Bead STANDARD (50-150µm)

6% B Agarose Bead STANDARD (50-150µm)

8% B Agarose Bead STANDARD (50-150µm)

10% B Agarose Bead STANDARD (50-150µm)

4% B Agarose Bead MACRO (150-350µm)

(1)

6% B Agarose Bead MACRO (150-350µm)

(1)

A-1020S-500

500 ml

A-1020S-1000

1l

A-1020S-10000

10 l

A-1040S-500

500 ml

A-1040S-1000

1l

A-1040S-10000

10 l

A-1060S-500

500 ml

A-1060S-1000

1l

A-1060S-10000

10 l

A-1080S-500

500 ml

A-1080S-1000

1l

A-1080S-10000

10 l

A-1100S-500

500 ml

A-1100S-1000

1l

A-1100S-10000

10 l

A-1040M-500

500 ml

A-1040M-1000

1l

A-1060M-500

500 ml

A-1060M-1000

10% B Agarose Bead MACRO(1) (150-350µm)

A-1100M-500 A-1100M-1000 A-1040F-250

4% B Agarose Bead FINE (20-50µm)

A-1040F-500

6% B Agarose Bead FINE (20-50µm)

4% BCL Agarose Bead STANDARD (50-150µm)

6% BCL Agarose Bead STANDARD (50-150µm)

8% BCL Agarose Bead STANDARD (50-150µm)

500 ml

250 ml

1l

A-1060F-250

250 ml

A-1060F-500

500 ml

A-1060F-1000

1l

A-1021S-500

500 ml

A-1021S-1000

1l

A-1021S-10000

10 l

A-1041S-500

500 ml

A-1041S-1000

1l

A-1041S-10000

10 l

A-1061S-500

500 ml

500 ml

25 ml

1l

VERY LOW Density AMINOETHYL 4 RAPID RUN™

4RR-AVL4-25

A-1061F-1000

4RR-AVL4-100

100 ml

HIGH Density AMINOETHYL 4 RAPID RUN™

4RR-AH1-25

25 ml

4RR-AH1-100

100 ml

LOW Density AMINOETHYL 6 RAPID RUN™

6RR-AL0-25

25 ml

6RR-AL0-100

100 ml

HIGH Density AMINOETHYL 6 RAPID RUN™

6RR-AM3-25

25 ml

6RR-AM3-100

100 ml

VERY LOW Density AMINOETHYL 6 RAPID RUN™ FINE

6RRF-AVL4-25

25 ml

6RRF-AVL4-100

100 ml

A-1270-100

100 g

A-1270-500

500 g

A-1280-100

100 g

A-1280-500

500 g

A-1290-100

100 g

A-1290-500

500 g

A-1300-25

25 g

A-1300-100

100 g

4RRS-500

500 ml

4RRS-1000

1l

4RRS-10000

10 l

6RRS-500

500 ml

6RRS-1000

1l

6RRS-10000

10 l

AGAROSE POWDER

LOW PRESSURE: GLYOXAL AGAROSE BEADS: BULK RESINS

LOW Density GLYOXAL 6BCL

HIGH Density GLYOXAL 6BCL

VERY HIGH Density GLYOXAL 6BCL GLYOXAL KIT

4BCL-GL0-25

25 ml

4BCL-GL0-100

100 ml

4BCL-GH1-25

25 ml

4BCL-GH1-100

100 ml

6BCL-GL0-25

25 ml

6BCL-GL0-100

100 ml

6BCL-GM3-25

25 ml

6BCL-GM3-100

100 ml

6BCL-GH1-25

25 ml

6BCL-GH1-100

100 ml

AGAROSES ABT Agarose LE

ABT Agarose HR

ABT Agarose GA

ABT Agarose LM

ACCESSORIES

GLYOXK-2

HIGH PRESSURE: GLYOXAL RAPID RUN™ BEADS: BULK RESINS LOW Density GLYOXAL 4 RAPID RUN™

4RR-GL0-25

25 ml

4RR-GL0-100

100 ml

HIGH Density GLYOXAL 4 RAPID RUN™

4RR-GH1-25

25 ml

4RR-GH1-100

100 ml

LOW Density GLYOXAL 4 RAPID RUN™ FINE

4RRF-GL0-25

25 ml

4RRF-GL0-100

100 ml

LOW Density GLYOXAL 6 RAPID RUN™

4RR-GL0-25

25 ml

4RR-GL0-100

100 ml

HIGH Density GLYOXAL 6 RAPID RUN™

4RR-GM3-25

25 ml

4RR-GM3-100

100 ml

LOW Density GLYOXAL 6 RAPID RUN™ FINE

4RRF-GL0-25

25 ml

4RRF-GL0-100

100 ml

EMPTY COLUMNS & EMPTY CARTRIDGES EMPTY MINI COLUMNS PLASTIC MINI COLUMNS

MC-100

100

SP-25

25

EMPTY SPIN COLUMNS PLASTIC SPIN COLUMNS EMPTY COLUMNS PLASTIC SMALL COLUMNS

CS-20

PLASTIC COLUMNS

C-50

50

PLASTIC XL COLUMNS

CXL-50

50

EB-Ctg1-5

5

20

A-1061S-1000

1l

A-1061S-10000

10 l

A-1081S-500

500 ml

A-1081S-1000

1l

A-1081S-10000

10 l

STOP PLUG

SEB-10

10

A-1101S-500

500 ml

SYRINGE CONNECTOR

SCEB-1

1

EMPTY FPLC 8 ml Columns

FPLC8-3

3

EMPTY FPLC 30 ml Columns

FPLC30-2

2

EMPTY FPLC 45 ml Columns

FPLC45-2

2

EMPTY FPLC 80 ml Columns

FPLC80-1

1

1l

4% BCL Agarose Bead MACRO (150-350µm)

A-1041M-1000

1l

6% BCL Agarose Bead MACRO(1) (150-350µm)

A-1061M-500

500 ml

A-1061M-1000

500 ml

A-1101M-1000

1l 250 ml

A-1041F-500

500 ml

A-1041F-1000

1l

Phone : +91-11-4875-4875

LOW PRESSURE: AMINOETHYL AGAROSE BEADS : BULK RESINS

EMPTY CARTRIDGES EMPTY CARTRIDGES (1ml) CARTRIDGES ACCESSORIES

EMPTY FPLC COLUMNS

4BCL-AVL4-25

25 ml

4BCL-AVL4-100

100 ml

4BCL-AH1-25

25 ml

4BCL-AH1-100

100 ml

LOW Density AMINOETHYL 6BCL

6BCL-AL0-25

25 ml

6BCL-AL0-100

100 ml

EMPTY FPLC COLUMNS SUPPLIES

HIGH Density AMINOETHYL 6BCL

6BCL-AM3-25

25 ml

FPLC Luer lock-male

FPLCLM-1

1

6BCL-AM3-100

100 ml

FPLC Luer lock-female

FPLCLF-1

1

AMINOETHYL LOW KIT

AMINOL-2

AMINOETHYL COMPLETE KIT

AMINOC-2

VERY LOW Density AMINOETHYL 4BCL HIGH Density AMINOETHYL 4BCL

1l

A-1101M-500

A-1041F-250

ACIDIC GROUPS

10 l 500 ml

4% BCL Agarose Bead FINE (20-50µm)

250 ml

AMINO GROUPS

HIGH Density GLYOXAL 4BCL

PACK SIZE

A-1061F-500

ELECTROPHORESIS

LOW Density GLYOXAL 4BCL

CAT. NO.

A-1061F-250

AFFINITY COUPLING

500 ml

A-1041M-500

10% BCL Agarose Bead MACRO(1) (150-350µm)

6% RAPID RUN™ Agarose Bead STANDARD (50-150µm)

1l

A-1040F-1000

10% BCL Agarose Bead STANDARD A-1101S-1000 (50-150µm) A-1101S-10000 (1)

4% RAPID RUN™ Agarose Bead STANDARD (50-150µm)

PRODUCT NAME

HIGH PRESSURE: AMINOETHYL RAPID RUN™ BEADS: BULK RESINS

HIGH PRESSURE: RAPID RUN™ AGAROSE BEADS

1l

LOW PRESSURE: CROSSLINKED AGAROSE BEADS 2% BCL Agarose Bead STANDARD (50-150µm)

6% BCL Agarose Bead FINE (20-50µm)

PACK SIZE

Email : [email protected]

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

Ready to use HPLC lectin Columns AffiSep® HPLC lectin columns are designed for the analysis, separation and purification of glycoproteins and other glycoconjugates containing lectin-specific carbohydrate residues. The columns are ready-to-use for operation with common HPLC or FPLC equipments and facilitate lectin affinity seperations of glycoproteins in a simple way for fast and reproducible results.

AffiSep® HPLC lectin columns specifications • Mean diameter particles: 75 µm

• Pore diameter: 1000 Å

COVALENTLY IMMOBILIZED LECTIN (mg/ml ADSORBENT)

AMOUNT OF BOUND GLYCOPROTEIN (ug/0.6 ml ADSORBENT)

Triticum vulgaris

~6

≥ 100

AffiSep® ConA column

Canavalia ensiformis

~15

≥ 150

AffiSep® LCH column

Lens culinaris

~3

≥ 135

AffiSep® PNA column

Arachis hypogaea

~3

≥ 125

AffiSep® AIL column

Artocarpus integrifolia

~3

≥ 215

AffiSep® VVL column

Hairy vetch

~3

-

011091 011092 011093

AffiSep® AAL column

Aleuria aurantia

~3

-

0.8 mL 1.6 mL 2.5 mL

011121 011122 011123 011124 011131 011132 011133 011134

AffiSep® SNA column

Sambucus nigra

~3

≥ 50

AffiSep® MAL column

Maackia amurensis

~3

≥ 125

0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL

CAT. NO.

PRODUCT DESCRIPTION

011031 011032 011033 011034 011041 011042 011043 011044 011051 011052 011053 011054 011061 011062 011063 011064 011071 011072 011073 011074 011082

AffiSep® WGA column

Phone : +91-11-4875-4875

LECTIN

Email : [email protected]

SIZE 0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL 1.6 mL

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

COVALENTLY IMMOBILIZED LECTIN (mg/ml ADSORBENT)

AMOUNT OF BOUND GLYCOPROTEIN (ug/0.6 ml ADSORBENT)

Ulex europaeus

~3

-

AffiSep® GNA column

Galanthus nivalis

~3

≥ 200

AffiSep® ECL column

Erythrina cristagalli

~5

≥ 100

CAT. NO.

PRODUCT DESCRIPTION

LECTIN

011141 011142 011143 011144 011151 011152 011153 011154 011161 011162 011163 011164

AffiSep® UEA column

SIZE 0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL 0.8 mL 1.6 mL 2.5 mL 4.0 mL

Storage: • Lectin columns should be stored equilibrated in adsorption buffer containing sodium azide at 4 °C. Handling of buffers and samples: • All buffer solutions should be filtered through 0,2 µm. • All samples should be solved in adsorption buffer and filtered through 0,2 µm. Protocol for column handling: • Equilibrate column for 30 min with adsorption buffer (flow: 0,8 mL/min) • Inject prepared sample solution and wash the column with adsorption buffer • Elute the sample for at least 4 min with the recommended lectin-specific elution buffer at 0,8 mL/min. • Equilibrate with adsorption buffer for 60 minutes for regeneration. Operational conditions for Lectin columns: • Max. column backpressure: 100 PSI (7 bar) • Recommended flow rate: 0,6 - 0,8 mL/min • Max. flow rate: 1,2 mL/min • Detection wavelength: 280 nm for glycoproteins, 300 nm for p-Nitrophenyl-carbohydrates • Range of pH: AffiSep®HPLC lectin columns can be operated between pH 4.0 and pH 8.0

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

Endotoxin Recovery Kits (Surfactant) Endo-RS® (Demasking of Endotoxin) Endo-RS® Endotoxin Recovery Kit is a novel sample preparation kit enabling complete endotoxin recovery in biopharmaceutical drug formulations containing non-ionic surfactants such as Polysorbate 20, Polysorbate 80 or Octylphenol etho xylates. Non-ionic surfactants are well known to cause Low Endotoxin Recovery (LER). Intensive studies of the phenomenon have shown that the physicochemical proper ties of surfactants render endotoxin molecules inaccessible (masked) for detection in such samples. The Endo-RS® Endotoxin Recovery Kit now provides the essential reagents and a detailed guideline for evaluating and establishing an optimized endo-toxin demasking protocol for each individual formulation containing surfactants. Applying the Endo-RS® Endotoxin Recovery Kit prior to endotoxin detection with the EndoLISA® Endotoxin Detection Assay will ensure FDA compliant and accurate results in spike validation studies and routine quality control testing.

Specifications & Advantages Ÿ Full quantitative endotoxin recovery in

biopharmaceutical samples containing surfactants Ÿ Demasking independent of masking time and

endotoxin concentration Ÿ All needed reagents for developing a formulationToolbox for demasking endotoxin in biopharmaceutical formulations containing surface active agents

specific protocol included in the kit Ÿ Detailed technical guidelines provided

Endo-RS® Protocol: 1. Screening:- Selection of the agents suitable for the specific formulation 2. Optimization:- Adjustment of protocol 3. Detection:- Quantification of demasked (accessible) endotoxin with the EndoLISA® assay PRODUCT NAME

CAT. NO.

Endo-RS® Endotoxin Recovery Kit

609065

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapentics

Endotoxin Detection Assays EndoZyme® (Enhanced Recombinant Factor C) EndoZyme® is a homogeneous fluorescence end-point assay for the quantitative determination of endotoxins (lipopolysaccharides). The assay is based on an improved recombinant Factor C (rFC), the endotoxin-specific principal receptor in the LAL enzyme cascade. With a dynamic range of 0-50 EU/ml and a sensitivity of 0.005 EU/ml, EndoZyme® offers state-of-the-art test performance. EndoZyme® specifically detects endotoxin thereby overcoming the problem with false-positive results due to βGlucans. EndoZyme® is particularly suited as endotoxin test for biopharmaceuticals, medical devices, water and environmental swabs. Better Alternative to LAL Test

Specifications & Advantages Ÿ Highly sensitive endotoxin detection assay Ÿ Broad measurement range 0.005-50 EU/ml Ÿ LPS-specific recombinant technology Ÿ No false-positive ß-Glucan reaction Ÿ Excellent reliability and lot-to-lot consistency Ÿ All needed reagents included in the kit Ÿ No animal material, therefore saving the

rFC Recommended as LAL alternative in EU & US Pharmacopoeia (EP,5.1.10) & (USP33,NF28,6.30)

EndoZyme® correlation with LAL-Assay

Correlation plot of EndoZyme® versus LAL assay where multiple LPS preparations and dilutions from different bacteria such as E. coli, Salmonella and Pseudomonas, including LPS of mutant strains, were compared. The endotoxin was determined quantitatively according to the manufacturer’s instructions and the results (EU/ml) were plotted against each other. This comparison shows that EndoZyme® correlates well with the commercially available LAL test, with a correlation coefficient (R2) of 0.9476.

Phone : +91-11-4875-4875

diminishing horseshoe crab population

EndoZyme® endotoxin detection standard curve

Standard curve of the EndoZyme® Assay; concent- rations of the LPS standard are plotted against the corresponding relative fluorescence signal. The log net fluorescence is proportional to the log endotoxin concentration and is linear in the 0.005-50 EU/ml range

PRODUCT NAME EndoZyme®- 192 tests

Email : [email protected]

CAT. NO. 609050

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

EndoLISA® (ELISA Based) EndoLISA® is a new method for determination of endotoxin (LPS) levels where high-affinity binding of endotoxin by LPS-specific phage protein is followed by endpoint fluorescence measurement. The microtiter plate -based assay format is one of the major advantage of EndoLISA®, as a result of which potentially interfering substances such as organic solvents, chaotropic substances and detergents are removed by a washing step. This enables the EndoLISA® user to precisely determine the endotoxin level without matrix effects, without the need for dilution and with a very broad measurement range. Better Alternative to LAL Test

Specifications & Advantages Ÿ Overcomes limitations of existing methods, such as the

need for extension dilution Ÿ Reduced matrix effects due to integrated washing step Ÿ Robust assay with excellent reproducibility Ÿ Broad measurement range (0.05-500 EU/ml) Ÿ Broad pH range (pH4 - pH9) Ÿ Less interference at high salt conditions e.g. NaCl, GdnHCl rFC Recommended as LAL alternative in EU & US Pharmacopoeia (EP,5.1.10) & (USP33,NF28,6.30)

Ÿ All reagents necessary to run the assay included in the kit Ÿ Saves the diminishing horseshoe crab population

EndoLISA® correlation with LAL-Assay EndoLISA® endotoxin detection standard curve

Correlation plot of EndoLISA® versus LAL assay where multiple LPS preparations and dilutions from different bacteria such as E. coli, Salmonella and Pseudomonas, including LPS of mutant strains, were compared. The endotoxin was determined quantitatively according to the manufacturer’s instructions and the results (EU/ml) were plotted against each other. This comparison shows that EndoLISA® correlates well with the commercially available LAL -assay, with a correlation coefficient of 0.9078.

Phone : +91-11-4875-4875

Standard curve of the EndoLISA® test; concentrations of the LPS standard are plotted against the relative fluorescence signal. EU, endotoxin unit.

PRODUCT NAME EndoLISA®- 192 tests

Email : [email protected]

CAT. NO. 609033

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

Endotoxin Removal Kits EndoTrap® (Affinity Chromotography Resins) EndoTrap® affinity chromatography for highly specific & efficient endotoxin (LPS) removal. The EndoTrap® ligand is a highly LPS-specific, completely non-toxic protein derived from bacteriophages. EndoTrap® removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rates. EndoTrap® can be used in column (gravity flow) or batch mode and is suitable for up-scaling on fully automated liquid-chromatographic systems.

Specifications & Advantages

Removal Rate >99.99%

Ÿ Best-in-class endotoxin binding capacity of 5,000,000 EU/ml Ÿ Removes more than 99.99%* of LPS Ÿ Excellent sample recovery rate > 95%* Ÿ Non-toxic phage-derived ligand, no cytotoxic effects Ÿ Re-use more than 10 times Ÿ Available in large quantities for up-scaling in

biopharmaceutical processes

EndoTrap ® red

EndoTrap ® blue

EndoTrap ® HD

LPS Binding capacity:

2,000,000 EU/ml resin

2,000,000 EU/ml resin

> 5,000,000 EU/ml resin

Reusability:

3 times

3 times

More than 10 times

PH sample buffer:

6-9

4-9

4-10

Suitable Buffers:

Calcium chelators

Suitable for high salt conditions (> 150 mM NaCl)

Suitable for high salt conditions (> 150 mM NaCl)

Sample Applications

Proteins, Peptides, Antibodies

Proteins, Peptides, Antibodies, DNA/RNA, Antigens,

Proteins, Peptides, Antibodies, DNA/RNA, Antigens, Plant extracts, Automated systems, Biomanufacturing processes

Plant extracts

*Measured values for BSA, MAK 33 (monoclonal antibody) and POD (peroxidase) with EndoTrap HD ®

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Quality & Purity of Biotherapeutics

PRODUCT NAME

DESCRIPTION

EndoTrap® red (1/1, 5/1, 10, 50, Bulk)*

CAT. NO.

For use with e.g. PBS buffered samples

321053, 321063 321064, 321075 321066

For use with e.g. HEPES, TRIS MOPS, MES, PIPES buffered samples

311053, 311063 311064, 311075 311066

For biotech/pharma evaluation and production processes

800053, 800063 800034, 800035 800036, 800037

12x8 well strips, 1 vial POD-Antibody 1 vial Standard 20 ml ABTS Substrate

800033

Available as pre-filled columns and settles resin.

EndoTrap® blue (1/1, 5/1, 10, 50, Bulk)* Available as pre-filled columns and settles resin.

EndoTrap® HD (1/1, 5/1, 10, 50, 250, Bulk)* Available as pre-filled columns and settles resin.

EndoTrap® Leakage ELISA (for EndoTrap® HD & EndoTrap® blue) *(1/1 = 1 x 1 ml column, 5/1 = 5 x 1 ml column, 10 = 10 ml resin etc.)

Endotoxin-free Reagents and Accessories EndoGrade® Microbiological contamination is a well recognized problem when research reagents are used in certain applications. In particular increased endotoxin levels often lead to artefacts and misinterpretations in cell culture or animal model experiments. With our EndoGrade® product range, we meet our customers demand for endotoxin-free reagents and accessories.

PRODUCT NAME

Phone : +91-11-4875-4875

PRODUCT

EndoGrade Glass Test Tubes 112 units per pack

800050

EndoGrade Water 30 ml 20 bottles per pack

607030

EndoGrade Water 100 ml 16 bottles per pack

607100

EndoGrade Water 500 ml 6 bottles per pack

607500

EndoGrade Ovalbumin 10 mg

321000

EndoGrade Ovalbumin 100 mg

321001

EndoGrade Ovalbumin 1000 mg

321002

Email : [email protected]

Fax : +91-11-2561-2008

®

Automation- Lab Essentials

Trinity V3- Industrial Microbiology System Automate and standardize zone reading and calculations for antibiotic potency

Trinity V3utilizes digital imaging to automate zone reading and calculations for antibiotic potency assays. An optional colony counting module is available. This complete industrial microbiology system is often used in pharmaceutical QA/QC microbiology laboratories.

Applications Antibiotic Potency Zone Reading • Follows USP, EP, BP and JP agar diffusion assay methods • 21 CFR Part 11 compliant

Colony Counting 3

Trinity V

Ÿ Instant digital reading & calculations

• Automatically counts bacterial, yeast colonies and virus plaque assays on agar plates

Ÿ IQ/OQ/PQ validation documents

• Saves 99% of reading time

Ÿ 21 inch touch screen

Ozone Sterilizer- unique PORTABLE laboratory sterilization system Ÿ Sterilizes surfaces & air spaces in multiple

areas & locations Ÿ Reduction or elimination of Bacteria,

Fungus, Viruses, Protozoa, Yeast & Algae Ÿ No harmful liquids, chemicals, UV rays, or

heat.

TM

Ozilla

Ÿ Keeps equipment, tools, and work areas

clean without chemical residue or damage Ÿ Auto De-ozonation at 240 cfm at the end of

a sterilization cycle

ENCLOSURE TYPE Incubator

Ozone Sterlizer

MIN. STERILIZATION TIME 2 hours

Tissue Culture Hood (3 feet wide)

4.5 hours

Tissue Culture Hood (4 feet wide)

6 hours

PRODUCT NAME

CAT. NO.

Tissue Culture Hood (5-6 feet wide)

8 hours

Ozilla™ Ozone Sterilizer, 220V

E400220

Room

12 hours

*The Ozilla sterilizer is designed for use in sealed cabinets or rooms, and is not to be used in open spaces where people and animals are present

Phone : +91-11-4875-4875

Email : [email protected]

Fax : +91-11-2561-2008

®

Automation- Liquid Handling

HTS DISPENSING

HTS WASHING

HTS COATING & WASHING

300 DMC Microplate Dispensing System

300-96WW Dual Microplate Washing System

TriFLEX Microplate Coating & Washing System

• 8 independent channels • Expandable to >1,000 plates/hour • 350 plates/hour@100uL dispense with