Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle

Media Peternakan, December 2015, 38(3):145-149 ISSN 0126-0472 EISSN 2087-4634 DOI: 10.5398/medpet.2015.38.3.145 Available online at http://medpet.jo...
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Media Peternakan, December 2015, 38(3):145-149

ISSN 0126-0472 EISSN 2087-4634

DOI: 10.5398/medpet.2015.38.3.145 Available online at http://medpet.journal.ipb.ac.id/

Accredited by DGHE No: 66b/DIKTI/Kep/2011

Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle R. Putri , R. Priyanto, A. Gunawan, & Jakaria*

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University Jalan Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia (Received 20-04-2015; Reviewed 04-06-2015; Accepted 08-10-2015)

ABSTRACT Calpastatin (CAST) gene is well known as an inhibitor of muscle protein degradation and relates to muscle growth and meat tenderness. The objective of this study was to determine the association of CAST gene with growth traits and carcass characteristics in Bali cattle. A number of data from 35 Bali bulls were collected from BPTU Bali Cattle to obtain growth traits, carcass characteristics, and blood samples. Polymorphism of CAST gene in Bali bulls was analyzed by using PCR-RFLP and DNA sequencing. The association of CAST gene with growth traits and carcass characteristics were analyzed by using General Linear Model (GLM). The result showed that there were two genotypes (GG and AG) of CAST gene with allele frequencies of 0.857 and 0.143, respectively, for G and A. Notably, mutation A to G occurred in 253 bp CAST fragment gene in Bali Cattle. Genotypes GG and AG of CAST gene significantly affected (P

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