Applied Subject
BIOTECHNOLOGY S C H O O L O F S C I E N C E & T E C H N O L O G Y 2 4 A U G 2 0 1 2
Introduc)on What is Biotechnology? = Biology + technology
Introduc)on Biotechnology ¡ a multi-‐disciplinary science that engages knowledge in biology and technological applications to improve human lives and the environment Provides the foundation for further studies in
biotechnology and related :ields
Why study Biotechnology?
Interes4ng Meaningful Rewarding
Interes)ng…
Meaningful…
There is no cure for mul4ple myeloma (cancer of the plasma cell) – stem cell therapy works & saved David Ong’s life.
Rewarding…
Probiotics – use of microbes to break down waste (instead of chemicals that pollute). As a result prawns grow quicker & bigger. No takers as prawn buyers were skeptical. Decided to set up own prawn farm. Probiotics worked very well. Last year, sales hit $1 million. This year pro:it will increase several-‐fold.
The Industry in Singapore • Biomedical sciences – The fourth pillar of Singapore’s economy
• Singapore’s Science & Technology Plan (STP2010) – S$13.5 billion fund
• A life sciences hub with world-‐class capabilities
Aims of Biotechnology – AS subject Obtain knowledge in biotechnology and understand
its applications
Aims of Biotechnology – AS subject Obtain knowledge in biotechnology and understand
its applications
Conduct hands-‐on experiments, projects and
research
Aims of Biotechnology – AS subject Obtain knowledge in biotechnology and understand
its applications
Conduct hands-‐on experiments, projects and
research
Understand recent advances in biotechnology and its
ethical and social implications
What topics will you learn? Sec3: Microbiology: study of microorganisms such as bacteria
Sec3: Gene Technology : cloning
What topics will you learn? Sec3: Animal cell culture: grow cells in the lab
Sec4: Plant tissue culture: grow genetically identical plants
What topics will you learn? 4
Sec : Applications of biotechnological in the areas of: ¡ ¡ ¡ ¡
forensic science medicine / healthcare environment and their ethical and social implications
Biofuel
GCE ‘O’ Level Exam Requirements The Biotechnology Applied Subject will be taught over 40 weeks spread over two years as follows: Sec 3: 30 weeks (90 hours) Sec 4: 10 weeks (30 hours) Total Lesson / Curriculum time: 120 hours There will be one lesson each week comprising a lecture or tutorial (1 hour) and laboratory (2 hours) sessions.
Teaching & Learning What to expect during lessons? ¡ small group lectures with interactive activities ¡ experiential & active learning ¡ hands-‐on laboratory sessions ¡ learner-‐centred learning projects ¡ :ield trips
GCE ‘O’ Level Exam Requirements Paper 1 -‐ multiple choice paper (45 min) Paper 2 -‐ written exam (1h 45 min) Paper 3 – structured project (15 hours over 5 weeks) ¡ students will work individually to design and conduct experiments, record observations, analyze data and write a report
Ques4ons?
Thank you!
Syllabus Outline (Sec 3) Unit 1. Introduction to Biotechnology • The Cell Unit 2. Gene Technology • DNA to RNA, RNA to Proteins • Recombinant DNA Technology • Genomics and the Human Genome Project Unit 3. Microbial Biotechnology • Bacteria Unit 4. Animal Cell Culture • Types of Animal Cell Cultures • Cell Culture Medium • Growth and Maintenance of Cells in Culture
Syllabus Outline (Sec 4) Unit 5. Plant Biotechnology • Plant Tissue Culture • Genetically Engineered Plants Unit 6. Biotech Applications Unit 6A: Forensic Science Unit 6C: Medical Biotechnology • DNA pro:iling • Biologics • Other applications Unit 6D: Environmental Unit 6B: Transgenic Animals • Gene transfer methods in animals Biotechnology • Bioremediation • Transgenic animals and their • Fermentation and biofuels applications
Biotechnology v.s. Biology
GCE ‘O’ Level Exam Requirements Theory Papers (Papers 1 and 2)
A Knowledge with Understanding, approximately 45% of the marks.
B Handling Informa)on and Solving Problems, approximately 55% of the marks. Project (Paper 3)
C Experimental Skills and Inves)ga)ons, 100% of the marks.
GCE ‘O’ Level Exam Requirements
Paper
1 2 3
Type of Paper
Duration
Marks
Multiple Choice 45 min 30 Structured questions 1 hr 45 min 80 Project 15 hours (100) Planning 15 Experimental Skills 45 Project Report 40
Weighting
20% 50% 30%
School-‐based Assessment Each semester: Continuous assessment ¡ Assignments and Laboratory experiments (50%)
Exams ¡ MCQ (20%) Structured questions (30%)
Preliminary Exam: ¡ MCQ (20%) Structured questions (30%)
Class size & Venue Small class size ¡ 20-‐24 students
Venue ¡ SST Biotechnology Laboratory
Instructors’ Profile Ngee Ann Polytechnic staff ¡ School of Life Sciences & Chemical Technology
Dr New Jen Yan Dr Susan Lee Dr Hedy Goh Specialists in the areas of microbiology, molecular
biology, cell culture and plant biotechnology
Skills you will obtain SKILLS ¡ Handle information, including search, select, interpret,
critique and evaluate data available to solve problems ¡ Plan and design experiments to solve scienti:ic problems
¡ Make and record observations, organize and present data
in a report
¡ Demonstrate hands-‐on skills and practical knowledge of
various biotechniques
¡ Communicate effectively through presentations
Learning Outcomes ATTRIBUTES ¡ Be aware of ethical and social issues related to
biotechnological applications ¡ Exercise responsibility, integrity and objectivity in scienti:ic
inquiry ¡ Develop an awareness of the role and impact of
biotechnology in everyday life
Learning Outcomes KNOWLEDGE
SKILLS
Understand the fundamentals of Effectively handle information, including gene technology and its search, select, interpret, critique, analyse and applications in genomics evaluate data available to solve problems De:ine and discuss microbial Plan and design experiments for projects or biotechnology and its solve scienti:ic problems applications Follow a sequence of instructions or Understand and apply the concepts of cell biology in animal experimental procedures to perform an experiment cell culture technology Understand and apply the Make and record observations, organize and concepts of plant tissue culture in present data in a report plant genetic modi:ications Demonstrate hands-‐on skills and practical Understand and appreciate the knowledge of biotechniques, aseptic cultures varied biotechnological and recombinant DNA technology applications in the areas of: forensic science Suggest possible improvements to existing medicine / healthcare methods or protocols environment and its ethical and social Communicate effectively through implications presentations
ATTRIBUTES
Be aware of ethical and social issues related to biotechnological applications Exercise responsibility, integrity and objectivity in scienti:ic inquiry Develop an awareness of the role and impact of biotechnology in everyday life
Teaching & Learning Out-‐of-‐class learning e-‐learning activities that promote self-‐discovery, self-‐learning and self-‐assessment
