APPENDIX 12 ELECTROPHORESIS WASTE DISPOSAL GUIDANCE

APPENDIX 12 ELECTROPHORESIS WASTE DISPOSAL GUIDANCE (For stock solutions, gels, non-sharp waste, and buffer solutions containing fluorescent dyes of ...
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APPENDIX 12

ELECTROPHORESIS WASTE DISPOSAL GUIDANCE (For stock solutions, gels, non-sharp waste, and buffer solutions containing fluorescent dyes of Ethidium Bromide, Propidium Iodide, Acridine Orange, and SYBR Green)

Chemical Hygiene Guide October 2009 Written February 23, 2009

Appendix 12 - 1 -

Chemical Hygiene Guide October 2009 Written February 23, 2009

Appendix 12 - 2 -

ELECTROPHORESIS WASTE DISPOSAL GUIDANCE (For stock solutions, gels, non-sharp waste, and buffer solutions containing fluorescent dyes of Ethidium Bromide, Propidium Iodide, Acridine Orange, and SYBR Green) This guidance has been developed to ensure proper disposal of ethidium bromide and other common fluorescent dyes (Gels and Solutions), which are commonly used in electrophoresis for the identification of DNA. Ethidium bromide is the most commonly used dye for this type of DNA work and the most toxic. Other common fluorescent dyes used in electrophoresis are; propidium iodide, acridine orange, SYBR Green, etc. When these materials become waste, they are managed by the same guidelines as ethidium bromide wastes. Note: Electrophoresis wastes containing acrylamide or polyacrylamide must be managed as hazardous waste, as per the Laboratory Hazardous Waste Disposal Policy and Procedures. The following three types of wastes are commonly generated from electrophoresis methods at the university: (which contain ethidium bromide and/or other common fluorescent dyes i.e. propidium iodide, acridine orange, SYBR green, etc.) • • •

Stock Solutions Gels and Contaminated Non-Sharp Debris, (i.e. gloves, tips, paper towels, etc.) Buffer Solutions (including filtration of these Solutions)

Stock Solutions (>10ug/ml concentration) 1.

2.

3.

Collect all electrophoresis stock solutions in an appropriately sized container, (REHS can provide 5-gallon carboy waste containers). The container cap should be closed securely when the container is not in use. Label all electrophoresis stock solutions with an Electrophoresis Waste Label, which is available from REHS. Indicate on the label that it is a liquid waste, and check off the appropriate constituent box on the label. Dispose of all electrophoresis stock solutions through REHS by utilizing the online request form for hazardous waste disposal at http://rehs.rutgers.edu. (If you are using the 5-gallon carboy provided by REHS, we will provide a replacement at the time of the waste pickup.)

Stock Solutions (10 ug/ml must be collected for disposal through REHS (follow the directions above for disposal of stock solutions), or



Buffer solutions containing >10 ug/ml can be filtered through a filtration system and the effluent can be drain disposed (see details below). If a spent buffer contains any constituents, other than the organic fluorescence dyes, which are not acceptable for drain disposal, then do not treat the waste. Manage the used buffer solution as a hazardous waste in accordance with the University’s Chemical Waste Disposal Procedures. Note: This does not allow for the drain disposal of filtered solutions at any locations that utilize a septic system such as research farms or marine research stations. All used buffer solutions from electrophoresis generated at these locations must be collected and disposed of by REHS.

Buffer Solutions ( 10 ug/ml Conc.

Collect for Disposal Through REHS

Collect for Disposal Through REHS

Filter to < 10 ug/ml

If on Septic System (typically farms & Stations)

If discharge to City Sanitary System

Collect for Disposal Through REHS

Drain Dispose