AMITECH, Inc.

From

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NATICIDE

A NEW FRAGRANCE FOR NATURAL PRESERVATION

A fragrance that preserves cosmetic formulations? This is the novel property of NATICIDE, a new vegetable based ingredient created by SINERGA research. NATICIDE has a mild fragrance, with a light and pleasant note of almond and vanilla that also acts as a fragrance enhancer. Its cosmetic function is to inhibit bacterial growth in cosmetic formulations. Market trends, in fact, have been turning to preservative-free formulations that still possess good stability, good organoleptic characteristics and, over all, good resistance to microbial contamination. Until now, these properties have always been an extremely important issue for formulators because cosmetic formulations must be protected against microbial contamination yet still be esthetically acceptable. NATICIDE is a new concept of multifunctional ingredient, allowing the formulation of safe, mild and preservative free cosmetics! CHARACTERISTICS NATICIDE is a blend of natural extracts formulated according to IFRA recommendations. It is a fragrance of vegetable origin, partly water soluble, very easy to handle. Its physical form is a clear liquid, colorless to yellow, with a pleasant, light sweet odor with vanilla and almond notes TECHNICAL DESCRIPTION Trade name: INCI Name (UE): INCI Name(USA): Appearance: Color: Odor: pH ( Hydroalcoholic sol. 1%): Solubility: Refractive Index:

NATICIDE® Parfum Fragrance Clear liquid Colorless to yellow Slight, characteristic of vanilla and almond 4.0 – 7.0 Up to 0.6% in water; total in glycol and alcohol 1.491 – 1.506

AMITECH, Inc. 11 Foundry Street Oxford, New Jersey 07863 Tele: (908) 453- 3743 FAX: (908) 453-3645 Email: [email protected]

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NATICIDE

A NEW FRAGRANCE FOR NATURAL PRESERVATION

The preliminary evaluation on NATICIDE has been done with the MIC Test (Minimum Inhibitory Concentration). The MIC determination is carried on by setting up a series of vials containing the product to test at different concentrations, and inoculating with a known quantity of a test microrganism. After the incubation period, the turbidity (resulting from microrganism growth) is evaluated. The lowest concentration of product that is able to inhibit bacterial growth is defined as the MIC, the Minimum Inhibitory Concentration. This test is extremely important in order to define the efficacy of the product and the sensitivity of different microrganisms to a defined product. . MICRORGANISM MIC Pseudomonas aeruginosae 5000 ppm Proteus vulgaris Escherichia coli Pseudomonas putida Serratia marcescens Enterobacter aerogenes Staphylococcus aureus

5000 ppm 5000 ppm 2500-5000 ppm 5000 ppm 5000 ppm 5000 ppm

MICRORGANISM Staphylococcus epidermidis Candida albicans Saccharomices cerevisiae Aspergillus niger

MIC 5000 ppm

Aspergillus flavus Penucillum notatum

2500 ppm 2500 ppm

2500 ppm 2500 ppm 2500 ppm

This preliminary antimicrobial activity test demonstrates interesting activity of NATICIDE in inhibiting microbial growth at very low concentrations. ANTIMICROBIAL ACTIVITY The antimicrobial activity of NATICIDE has been tested in detail by a simulated microbial attack, better known as CHALLENGE TEST. The method used for the Viable Count is the one according to Farmacopea Ufficiale Italiana IX ed., and the Direttiva CEE 76/768/CEE dated 27.07.1976. The method used for the challenge test is the one according to the European Pharmacopoeia III ed. modified. 2

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NATICIDE

A NEW FRAGRANCE FOR NATURAL PRESERVATION

The challenge test allows the evaluation of the real capacity of not only preventing microbial contamination, but reducing it to where it becomes safe for human use once contamination has occurred. The following is the technical information regarding the challenge tests performed on NATICIDE.

ATCC (American Type Culture Collection)

BACTERIA GRAM + Staphylococcus aureus Staphylococcus epidermidis

6538 12228

BACTERIA GRAM – Pseudomonas aeruginosa Pseudomonas putida Escerichia coli Enterobacter aerogenes Proteus vulgaris Serratia marcescens

9027 49128 8739 13048 13315 8100

YEAST Saccharomices cerevisiae Candida albicans

2366 10231

MOULD Aspergillus niger Aspergillus flavus Penicilium notatum

16404 9643 9178

INOCULUM inoculum quantity 1% v/v (1ml/100ml) 108 -109 u.f.c./ml

u.f.c./m

107 -108 u.f.c./ml

106 -107 u.f.c./ml

The total Viable Count after 7, 14, & 28 days of incubation to determine the microbial survival LEGEND Microbial Microbial Microbial Microbial

survival survival survival survival

< 0.2%