A LTM story in nanotechnology

D. Peyrade CNRS Research Director Team leader Micronanotechnology for Health Co-founder of Smart Force Technologies © LTM-CNRS c/o CEA-LETI, Grenoble David PEYRADE | [email protected]

J. Cordeiro Président, co-founder Smart Force Technologies © SAS www.smartforcetechnologies.com ARC6 1 april 2016 1

Outline • LTM : The beginning of the story…. • Smart Force Technologies ©

• The future for LTM & SFT

David PEYRADE | [email protected]

ARC6 1 april 2016 2

The boom of colloidal matter •

Mastering chemical synthesis - Complexity of materials (low cost)

•

Physics properties are specifics - S/V – confinement elect/photon

•

How to integrate this material?

Miroelectronic technologie not suitables Litho, etch, deposition… Bawendi

-

Broad range of application domains Particles brownian motion

Strategies to exploit particles physical properties Interfaces  Assembly

Outside forces Manipulate 3 FGRAD  V opt/ m Kopt / CM E 2 2 Particle /Milieu polarisable Champ électrique alternatif / Electromag. Attraction/Repulsion

t=10s T=25°C

Attraction

eau

Particule PS 500 nm Particules 1 nm -10 µm Force [10 nN …pN]

Hydrodynamic, Capillary, gravitationnal David PEYRADE | [email protected]

Electrocinetic

Optic ARC6 1 april 2016 3

Thermodynamic assembly : From physics to technology Innovations

Thermodynamic

Illustrations Goutte Goutte

Q ~100ml/min 10 µm 10 µm

Substrat Substrat

-Exploit hydrodynamic force  Aspiration flux -Maximise capillary forces  surface functionnalisation -Mastering the front displacement  fluidic cell

2 Patents : Principle/cell

Préconcentration

Assemblage 5 µm

1µm Polystyrène

3 PhDs, 3 ANR, 11 RICL

Filling factor>99%

>1 Million /cm2

1 European demonstration (2006) GenericTechnology (material/substrate) to integrate collectivly colloidal particles Thermodynamic assembly: An elementary technology inserted in micro-nanotechnologies (litho, etch, NIL…) David PEYRADE | [email protected]

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Application domains Particles size: 5 nm à 10 µm

TICs

Plasmonic

Photonic

Magnétism

1 µm

200 nm

Nanoantenna (dimers/chains)

Guiding/confinement focalisation sub-l

Plasmons 3D

Coll. CEMES ITODYS, ESPCI …

Biology

5nm domains

White light emitters

2 ANR 11 RICL (APL, JVSTB, J. Phys Chem C…) 2 Patents 2 thèses

Molecular scale (nm)

Cellular scale(µm) S. epidermidis

15 µm

100 nm

Coll. LMGP

DNA combing

5 µm Coll. Clinatec

Exosomes (illness sentinel)

3 µm Coll. LETI

Bacteria 1 ANR 1 Carnot, 3RICL, 1 thèse

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Industrial transfert • SMART FORCE : Integration of colloidal particles on-chip From fundamental research to a commercial solution Fundamental research

Concept proof /Prototype

TRL 1

Operationnal system

Commercialization (LTM)

TRL 7

TRL 9

TRL 3

Unit

X3

X2

2011

ISA

Consommables

Microfluidic chamber

2006

1st sell

Functionnalised substrates

3 PhDs, 2 ANRs,11 RICL,2 Patents,3 prototypes

Stand conference

2015

• The « First step" team 2012-2015

D. Peyrade CNRS, LTM

M. Zelsmann CNRS,LTM

E. Picard CEA

K. Berton, CNRS,LTM

K. Berton, doct.,LTM

Human adventure (facts, skills…) Entrepreneurial model : organic growth David PEYRADE | [email protected]

ARC6 1 april 2016 6

SMART FORCE: THE SOLUTION

David PEYRADE | [email protected]

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SMART FORCE SOLUTION Research activity Successfull companies experience : attocube Imina … Prototype  commercial product Subcontractors : Atelier chartreuse Supernova… Intelligent microfluidic head

Marketing: product brochure ..booth Key opinion leader : conferences…

SMART FORCE

David PEYRADE | [email protected]

ARC6 1 april 2016 8

PROMOTING SMART FORCE SOLUTION

Salon Nanotech, Japon 2014

Lyon, March 2015 MNE 2014, Lausanne

Meet new ecosystem : academic collaborators, distributors, start-up, industrial First sell  Ready for the creation of Smart Force Technologies David PEYRADE | [email protected]

ARC6 1 april 2016 9

Operationnal team SATT Grenoble

Julien CORDEIRO, PhD 2010-2013: Thèse LTM CFA, Optique, Prototypage 2012: Challenge First Step CEA 2014-2015: Projet Smart Force

Incubation: oct 2015

Olivier LECARME, PhD 2008-2011: Thèse LTM CFA, Optique, Informatique 2012-2014: Post-doc Sapporo Optique, Cellule solaires 2014-2015: Projet Smart Force

Smart Force Technologies is a young company born from French laboratories LTM-CNRS & CEA Smart Force Technologies © SAS www.smartforcetechnologies.com

Scientific advisor D. Peyrade LTM-CNRS E. Picard CEA

Julien CORDEIRO | [email protected] David PEYRADE | [email protected] www.smartforcetechnologies.com

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Natural evaporation

Controlled evaporation

1 µm

-

1 µm

Predetermined and reproductible deposition

Individual caracterisation of structural and physico-chemical properties of nanoparticles

Random deposition  agregates = “Finding a needle in a haystack” Julien CORDEIRO | [email protected] David PEYRADE | [email protected] www.smartforcetechnologies.com

Heterogen material integration

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Smart Force Unit

Smart Force Kit

Microfluidic cells

Patent n° 14 54166 Product Key Features

Software

nm scale resolution NanoMicrometric Assembly Dynamic visualisation 1 cm2 chip surface 2D and 3D assembly Portable system

Assembly system

Fisrt customer delivery Julien CORDEIRO | [email protected] David PEYRADE | [email protected] www.smartforcetechnologies.com

KTH Royal Institut of Technology Stockholm, Sweden ARC6 1 april 2016 12

Products Roadmap 2016

2017

2018

OFFER SMART FORCE

OFFER SMART PRINT

OFFER SMART FORCE PROD.

Equipment: 40k€ Consummable: 1k€ Prestation: 2k€

Equipment: 15k€ Consumable: 1k€

RESEARCH LAB EDUCATION 10 000 worldwide

Equipment: 80k€

INDUSTRIALS 14 000 affiliés nano  Telecommunication  Energy  Health

Julien CORDEIRO | [email protected] David PEYRADE | [email protected] www.smartforcetechnologies.com

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INNOVATIVE MICROBEAD BASED TECHNOLOGY FOR ANTIBODIES IGE DETECTION Microbead based IgE antibody detection by magnetic separation

A. Leonard (PhD)

Microbead PS 10μm Primary Antibody Magnetic microbead 4,5μmSecondary antibody

PS-IgE

Mag-Anti-IgE PS-COOH

Microbead complex

PS-IgE PS-COOH

No filtering 50/50

National research program

LTM Grenoble) Immunolab (Lyon) Smart Force Technologies ©

Magnetic fishing 90/10

AAP 2015 Coord. LTM

Innovative microbead technology for allergy diagnosis

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ASSEMBLY FOR MOLECULAR DIAGNOSIS Microbeads technology

Magnetic fishing

Microbilles

Polystyrene beads (10µm) IgE

Biochip

50 µm 1 mm

Magnetic bead Anti-IgE

Sensitivity , integration, cost

Thermodynamic assembly

Reactive beads collection IgE

Lens-free imaging analysis

FOV 20 mm X 15 mm / Resolution 5 µm

Assemblage (billes + IgE)

Allergens tests – Patient serum ISAC Chip

Food Insects Drugs

Ara h2 Arachid validation

Pollen & … plants Coll. Pharmaciens

Partners LTM –coord (Grenoble) Immunolab hospital (Lyon) AOMC-Pharmacology (Toulouse) CEA INAC (Grenoble) Smart Froce Technologies © (Grenoble)

Test on patient serum (3000 human allergens / cross-allergy)

Synergy of technologies Microbeads solutions for molecular diagnosis  Health applications: allergy, oncology…

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Conclusion • A LTM sucessfull story in nanotechnology • Human adventure • Explore an entrepeunarial model (organic – lab sell ) - The spin-off company Smart force technology © is launched • Institutional support (CEA CNRS LTM INAC SATT) • Ride an academic-industrial partnership Transfert new technologies LTM/MNH

Health Applications Synergy

Company students Assembly

2015

David PEYRADE | [email protected]

Lithography

2016

Imaging

Allergy diagnosis

2018 ARC6 1 april 2016 16

David PEYRADE | [email protected]

ARC6 1 april 2016 17

From prototype to final product Le prototype

Le produit final

Design du prototype Sous-traitance : Atelier de Chartreuse

+

Cellules microfluidiques Fabrication en interne du prototype David PEYRADE | [email protected]

Brevet FR14 54166 CEA/CNRS/UJF ARC6 1 april 2016 18