th
8 Workshop on Innovative Mouse Models
June 11-12, 2015 Leiden University Medical Center Albinusdreef 2, 2333 ZA Leiden
Organization Jos Jonkers, The Netherlands Cancer Institute, Amsterdam Paul Krimpenfort, The Netherlands Cancer Institute, Amsterdam Werner Müller, University of Manchester, Manchester, UK Els Robanus Maandag, LUMC, Leiden Hein te Riele, The Netherlands Cancer Institute, Amsterdam Marian van Roon, InnoSer Nederland B.V., Lelystad Sjef Verbeek, LUMC, Leiden
Cover art: Drs. Bernd Dworniczak and Petra Pennekamp, Institute for Human Genetics, Münster, Germany. Abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and cited as such only with consent of the author(s).
Topics CRISPR/Cas9-assisted gene modification, recombineering, novel imaging methods, organoids, HIS mouse, mouse reproductive technology
Keynote speakers Shengdar Tsai Joung lab, Massachusetts General Hospital, Charlestown, USA Defining and improving the specificity of the CRISPR/Cas9 nuclease platform Kosuke Yusa The Wellcome Trust Sanger Institute, Hinxton, UK Genome-wide recessive genetic screening with a lentiviral CRISPR-guide RNA library Peter Friedl University of Texas, Houston, USA Multi-scale intravital microscopy of the tumor-stroma interface and progression Jacco van Rheenen Hubrecht Laboratory, Utrecht, the Netherlands Intravital imaging of cancer plasticity at subcellular resolution Julien Villaudy Spits lab, AIMM Therapeutics, Amsterdam, the Netherlands Humanized immune system (HIS) mice as powerful preclinical in vivo models Matthias Heidenreich Zhang lab, Broad Institute of MIT and Harvard, Cambridge, USA RNA-guided genome engineering: new expansion of Cas9 toolbox and in vivo applications Francis Stewart Technical University Dresden, Germany Combining CRISPR/Cas9 with recombineering Marc van de Wetering Clevers lab, Hubrecht Laboratory, Utrecht, the Netherlands Lgr5 stem cells and organoids Toru Takeo Kumamoto University, Kumamoto, Japan Improving the efficiency of mouse reproductive technology for connecting the world Ivo Huijbers Berns lab, Netherlands Cancer Institute, Amsterdam, The Netherlands Implementation of improved stem cell culture and new genome engineering technologies in a transgenesis core facility Niels Geijsen Hubrecht Laboratory, Utrecht, the Netherlands Efficient intracellular delivery of native proteins
Meeting Venue Leiden University Medical Center Albinusdreef 2, Leiden - Building 1, Lecture Hall 5
How to get there by car: Coming from Rotterdam (A13, A4): - Take the A13 and then the A4 towards Amsterdam; exit at junction 6a (ZoeterwoudeRijndijk, Leiden-Oost) and turn left. At the end of the road, turn right and follow the signposting ("HZiekenhuizen"). Coming from Utrecht (A12, N11): - Take the A12 and at Bodegraven take the exit for Leiden (N11). At the end of the N11, turn right and follow the signposting ("HZiekenhuizen"). Coming from The Hague (N44, A44): - From The Hague, take the N44 (which turns into the A44) and exit it at junction 8 (Leiden, Noordwijk, Katwijk); turn right and follow the signposting ("H-Ziekenhuizen"). Coming from Amsterdam (A4, A44): - Take the A4 and then the A44 in the direction of Leiden-West. Exit the A44 at junction 8 (Leiden, Noordwijk, Katwijk, N206), turn left and go under the viaduct. Follow the signposting ("H-ziekenhuizen”) Parking The LUMC has a multi-storey car park. Parking rates and more information are published at http://www.lumc.nl Public transport The LUMC is located behind Leiden’s Central Station and is within walking distance. All regional buses to Leiden stop at the Central Station in Leiden. Some buses stop in front of the LUMC, at Bargelaan (behind the station). All buses stop at the bus station in front of the station. The Directorate for Education and Study Programmes and the Continuing Education Office (Boerhaave Committee) are located in Building 3 of the LUMC.
Functional locations
Restaurant Luxor, (Stationsweg 19; 2312 AS Leiden; tel. +31 (0)71 - 514 9491)
Golden Tulip hotel, (Schipholweg 3; 2316 XB Leiden; tel. +31 (0)71 - 408 3500)
Ibis hotel, (Stationsplein 240; 2312 AR Leiden; tel. +31 (0)71 - 516000)
Groundplan LUMC Research Building Research Building
Research Building
Lecture Hall 5 (First floor)
Lunch/ Exhibition
Galeria Staircase
Parking
Main Entrance
Railway Station
Building 1, Lecture Hall 5 (Albinusdreef 2 2333 ZA Leiden, tel. +31 (0)71 – 526 9111)
Program IMM 2015 LUMC Leiden - Lecture hall 5
Thursday June 11, 2015 09.00 Registration & welcome with coffee 09.55-10.00 Opening
Sjef Verbeek
5’
10.00-12.15 Session 1: CRISPR/Cas9 genome engineering I
Chair: Sjef Verbeek
10.00
10.45 11.30 11.45 12.00
Shengdar Tsai (Joung lab, Mass Gen Hospital, Charlestown, USA)
45’
Kosuke Yusa (Wellcome Trust Sanger Institute, Hinxton, UK)
45’
R Kühn (Max Delbrueck Center, Berlin, Germany)
15’
M Pendas (Centro de Investigacion del Cancer, Salamanca, Esp)
15’
BJ Davies (University of Oxford, Oxford, United Kingdom)
15’
Defining and improving the specificity of the CRISPR/Cas9 nuclease platform Genome-wide recessive genetic screening with a lentiviral CRISPR-guide RNA library
Using traffic light-reporter cells and mice to analyse CRISPR/Cas9 -induced double-strand break-repair by HDR or NHEJ Functional analysis of cohesin STAG2 by in vivo electroporation of mouse testis with CRISPR/CAS9 system Efficient CRISPR/Cas9 genome engineering using zygotes and embryos derived from Cas9 overexpressing transgenic mice
12.15-14.00
Lunch + exhibition
14.00-15.30 Session 2: Imaging
Chair: Jos Jonkers
14.00
Peter Friedl (University of Texas, Houston, USA)
45’
14.45
Jacco van Rheenen (Hubrecht Institute, Utrecht, NL)
45’
15.30-16.00
Multi-scale intravital microscopy of the tumor-stroma interface and progression Intravital imaging of cancer plasticity at subcellular resolution Coffee/tea Break + exhibition
16.00-16.45 Session 3: Imaging continued
Chair: Paul Krimpenfort
16.00
S Warming (Genentech, Inc., South San Francisco, USA)
15’
16.15
NK Kruse (Helmholtz Centre Infection Res, Braunschweig, Ger)
15’
D Stevenson (Beatson Institute for Cancer Res, Glasgow, UK)
15’
16.30
Kaleida: a new and versatile Cre-inducible multi-color lineage tracing allele
Epigenetic roadblocks towards an tetracycline inducible mouse model
A transgenic mouse model expressing an inducible E-CadherinEGFP fusion protein from the wt locus
16.45-17.30 Session 4: Humanized models
Chair: Paul Krimpenfort
16.45
Julien Villaudy (Spits lab, AIMM Therapeutics, Amsterdam, NL)
17.30
Drinks and snacks Dinner at Restaurant Luxor in Leiden (Stationsweg 19; tel. +31 (0)71 - 514 9491)
Humanized immune system (HIS) mice as powerful preclinical in vivo models
45’
Friday June 12, 2015 09.00-10.30 Session 5: CRISPR/Cas9 genome engineering II
Chair: Els Robanus Maandag
09.00
09.45
Matthias Heidenreich (Zhang lab, Broad Institute, Cambridge, 45’ USA)
RNA-guided genome engineering: new expansion of the Cas9 toolbox and in vivo applications Francis Stewart (Technical University, Dresden, Germany)
Combining CRISPR/Cas9 with recombineering
10.30-11.00
45’
Coffee Break + exhibition
11.00-12.15 Session 6: CRISPR/Cas9 applications
Chair: Hein te Riele
11.00 11.45 12.00
Marc van de Wetering (Clevers lab, Hubrecht Laboratory, Utrecht, NL)
45’
D Seruggia (CNB-CSIC, Madrid, Spain)
15’
SJ Erkeland (Erasmus MC, Rotterdam, NL)
15’
Lgr5 stem cells and organoids
Interrogating the non-coding mouse genome using CRISPR-Cas9 Interstrand cross-link induced miR139-3p and miR199a-3p have opposite roles in hematopoietic cell-expansion and leukemic transformation
12.15-13.15
Lunch + exhibition
13.15-14.45 Session 7: Transgenic animal facilities
Chair: Marian van Roon
13.15
Toru Takeo (Kumamoto University, Kumamoto, Japan)
45’
14.00
Ivo Huijbers (Netherlands Cancer Inst., Amsterdam, NL)
45’
Improving the efficiency of mouse reproductive technology for connecting the world Implementation of improved stem cell culture and new genome engineering technologies in a trangenesis core facility
14.45-15.15 15.15-16.15
Coffee/tea Break + exhibition Session 8: Applications/models
Chair: Werner Müller
15.15
Niels Geijsen (Hubrecht Institute, Utrecht, The Netherlands)
30’
15.45
LT Teboul (MRC Harwell, Didcot, United Kingdom)
15’
SA Annunziato (NKI-AVL, Amsterdam, Netherlands)
15’
16.00
Efficient intracellular delivery of native proteins
Engineering the mouse genome using CRISPR/Cas9 Rapid testing of candidate recessive factors in ILC by CRISPRmediated in vivo gene editing
16.15-16.45 Forum discussion: the impact of CRISPR/Cas9 technology 30’
Moderators: Hein te Riele and Werner Müller
16.45
Closing remarks
Sjef Verbeek
5’
